pAAVLINK-EF1a-HA-5'-KAT6B

LOCUS       pAAVLINK-EF1a-HA-5'-KAT6B                7359 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..4499
                     /codon_start=1
                     /label=KAT6B
                     /translation="MVKLANPLYTEWILEAIQKIKKQKQRPSEERICHAVSTSHGLDKK
                     TVSEQLELSVQDGSVLKVTNKGLASYKDPDNPGRFSSVKPGTFPKSAKGSRGSCNDLRN
                     VDWNKLLRRAIEGLEEPNGSSLKNIEKYLRSQSDLTSTTNNPAFQQRLRLGAKRAVNNG
                     RLLKDGPQYRVNYGSLDGKGAPQYPSAFPSSLPPVSLLPHEKDQPRADPIPICSFCLGT
                     KESNREKKPEELLSCADCGSSGHPSCLKFCPELTTNVKALRWQCIECKTCSACRVQGRN
                     ADNMLFCDSCDRGFHMECCDPPLSRMPKGMWICQVCRPKKKGRKLLHEKAAQIKRRYAK
                     PIGRPKNKLKQRLLSVTSDEGSMNAFTGRGSPGRGQKTKVCTTPSSGHAASGKDSSSRL
                     AVTDPTRPGATTKITTTSTYISASTLKVNKKTKGLIDGLTKFFTPSPDGRRSRGEIIDF
                     SKHYRPRKKVSQKQSCTSHVLATGTTQKLKPPPSSLPPPTPISGQSPSSQKSSTATSSP
                     SPQSSSSQCSVPSLSSLTTNSQLKALFDGLSHIYTTQGQSRKKGHPSYAPPKRMRRKTE
                     LSSTAKSKAHFFGKRDIRSRFISHSSSSSWGMARGSIFKAIAHFKRTTFLKKHRMLGRL
                     KYKVTPQMGTPSPGKGSLTDGRIKPDQDDDTEIKINIKQESADVNVIGNKDVVTEEDLD
                     VFKQAQELSWEKIECESGVEDCGRYPSVIEFGKYEIQTWYSSPYPQEYARLPKLYLCEF
                     CLKYMKSKNILLRHSKKCGWFHPPANEIYRRKDLSVFEVDGNMSKIYCQNLCLLAKLFL
                     DHKTLYYDVEPFLFYVLTKNDEKGCHLVGYFSKEKLCQQKYNVSCIMIMPQHQRQGFGR
                     FLIDFSYLLSRREGQAGSPEKPLSDLGRLSYLAYWKSVILEYLYHHHERHISIKAISRA
                     TGMCPHDIATTLQHLHMIDKRDGRFVIIRREKLILSHMEKLKTCSRANELDPDSLRWTP
                     ILISNAAVSEEEREAEKEAERLMEQASCWEKEEQEILSTRANSRQSPAKVQSKNKYLHS
                     PESRPVTGERGQLLELSKESSEEEEEEEDEEEEEEEEEEEEDEEEEEEEEEEEEEENIQ
                     SSPPRLTKPQSVAIKRKRPFVLKKKRGRKRRRINSSVTTETISETTEVLNEPFDNSDEE
                     RPMPQLEPTCEIEVEEDGRKPVLRKAFQHQPGKKRQTEEEEGKDNHCFKNADPCRNNMN
                     DDSSNLKEGSKDNPEPLKCKQVWPK"
     misc_feature    954..959
                     /label=SDS
     misc_feature    2550..2555
                     /label=SDS
     misc_feature    2553..2558
                     /label=SDS
     misc_feature    3807..3812
                     /label=SDS
     misc_feature    3966..3971
                     /label=SDS
     misc_feature    3969..3974
                     /label=SDS
     misc_feature    3972..3977
                     /label=SDS
     misc_feature    3981..3986
                     /label=SDS
     misc_feature    3984..3989
                     /label=SDS
     misc_feature    3996..4001
                     /label=SDS
     misc_feature    4023..4028
                     /label=SDS
     misc_feature    4160..4165
                     /label=SDS
     intron          4500..4540
                     /label=Splicing Donor
     misc_feature    4544..4577
                     /label=lox2272-JT1571
     repeat_region   4622..4762
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4622..4751
                     /label=AAV2 ITR
     rep_origin      4837..5292
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5574..5678
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5679..6539
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6710..7298
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat ggtaaaactt gcaaacccac tttatacaga
      781 gtggattctt gaagctatac agaaaataaa aaagcaaaag caaaggccct ctgaagagag
      841 aatctgccat gcggtcagta cttcccatgg gttggataag aagacagtct ctgaacagct
      901 ggaactcagt gttcaggatg gctcagttct caaagtcacc aacaaaggcc ttgcctccta
      961 taaggaccca gacaaccctg ggcgcttttc atcagttaaa ccaggcactt ttcctaagtc
     1021 agccaagggg tctagaggat catgtaatga tctccgcaat gtggattgga ataaactttt
     1081 aaggagagca attgaaggac ttgaggagcc gaatggctcc tccctgaaga acatagagaa
     1141 gtatctcaga agtcaaagtg atctcacaag caccaccaac aacccagcct ttcagcagcg
     1201 gctgcgactg ggggccaaac gcgctgtgaa taatgggagg ttactgaaag acggaccgca
     1261 gtacagggtc aattatggga gcttagatgg caaaggggca cctcagtatc ccagtgcatt
     1321 cccatcctcg ctcccacctg tcagccttct accccatgag aaagaccagc cccgtgctga
     1381 tcccattcca atatgtagct tctgtttggg gactaaagaa tcaaatcgtg aaaagaaacc
     1441 agaagaactc ctctcttgtg cagattgtgg cagtagtgga cacccatcct gtttgaaatt
     1501 ttgtcctgaa ttaacaacaa atgtaaaggc cttaaggtgg cagtgcatcg aatgcaagac
     1561 atgcagtgcc tgtagagtcc aaggcagaaa tgctgataat atgctttttt gtgattcctg
     1621 tgatagagga tttcatatgg aatgctgtga cccaccactt tccagaatgc caaaagggat
     1681 gtggatttgc caagtctgca gaccaaagaa aaagggaaga aaactacttc atgagaaagc
     1741 tgcacaaata aaacgacgat atgcaaaacc cattggacga ccgaaaaata aattaaagca
     1801 acgattgttg tctgtaacca gtgatgaagg atccatgaat gcattcacag gaagggggtc
     1861 acctggtagg ggtcaaaaga ctaaagtctg taccacacct tcatctggtc atgctgcatc
     1921 tgggaaggac tcaagcagca gattggctgt tacagacccc actcggcctg gtgccaccac
     1981 caaaatcacc accacctcca cctacatttc tgcctctaca cttaaagtta acaagaaaac
     2041 caaagggctc attgatggcc ttactaagtt ttttacacca tcacctgatg gtcgcagatc
     2101 acgaggtgaa attatagact tttcaaagca ctatcgtcca aggaaaaagg tctctcagaa
     2161 acagtcatgc acttctcatg tgttggctac aggtaccaca caaaagctaa aacctccacc
     2221 ttcttcactt ccacccccaa cccccatctc cggtcagagc cccagttcac aaaagtccag
     2281 cacggccact tcttctccct ctccccagag ttcttccagc cagtgcagtg tgccctccct
     2341 gagcagcctt accactaaca gccagctgaa ggcactcttt gatgggcttt ctcatatcta
     2401 taccactcag ggacagtctc gcaaaaaggg acacccgagt tatgcaccac ccaaacgtat
     2461 gcgtcgtaaa actgaattat cttccacggc aaaatctaaa gcccacttct ttggcaaaag
     2521 agatattaga agtcggttta tttctcactc ctcctcctct agctggggga tggctagagg
     2581 aagtattttt aaagcaattg ctcacttcaa gcgaacaact ttccttaaaa agcacaggat
     2641 gctaggcaga ttaaaatata aagtgacccc tcagatgggg accccctcac cagggaaggg
     2701 gagcttgaca gacggaagga ttaaacctga tcaggatgat gatactgaaa taaaaataaa
     2761 catcaaacaa gaaagtgcag atgtaaatgt gattggaaac aaggatgtcg ttactgaaga
     2821 ggatttggat gtttttaagc aggcccagga actttcttgg gagaaaatag agtgtgagag
     2881 tggggtggaa gactgtggcc ggtacccttc tgtgattgaa tttggtaaat atgaaatcca
     2941 aacctggtac tcctcgcctt acccacagga atatgcaaga ttaccaaagc tttacctgtg
     3001 tgaattctgt cttaaatata tgaaaagtaa aaatattttg ctaagacact ccaagaagtg
     3061 tggatggttt catcctccag caaatgaaat ttaccgaagg aaagaccttt cagtatttga
     3121 ggttgatggg aatatgagca aaatttattg ccaaaacctt tgcttgttag ccaagctctt
     3181 cctggaccac aaaacgttgt attatgatgt cgagccattc cttttttatg tccttacaaa
     3241 aaatgatgaa aagggctgtc atctggttgg atacttctct aaggaaaagc tttgccagca
     3301 gaagtataat gtctcctgca taatgatcat gccccagcac caaaggcaag gatttggacg
     3361 gtttctcatt gatttcagct atttgctttc tagaagagaa ggccaagcag ggtctcctga
     3421 aaagcctctc tccgatctgg gccgtctctc ctacctggca tattggaaga gcgtcatctt
     3481 ggagtatctc taccaccacc atgagaggca catcagcatc aaggcaatta gcagagcgac
     3541 gggcatgtgc ccacatgaca ttgccaccac tctgcagcac ctccacatga tcgacaagag
     3601 agatggcaga tttgtcatca ttagacggga aaagttgata ttgagccaca tggaaaagct
     3661 gaaaacctgt tccagagcca atgaacttga tccagacagt ctgaggtgga ccccaatttt
     3721 aatttctaat gctgcagtgt ctgaagaaga gcgagaagct gagaaagagg ctgagcggct
     3781 aatggaacaa gctagctgct gggagaagga ggaacaagaa atcctgtcaa ctagagctaa
     3841 cagtaggcaa tcacctgcaa aagtacaatc gaaaaataaa tatttgcatt ccccggagag
     3901 ccggccagtc acaggggagc gagggcagct gctggagctg tctaaagaga gcagtgaaga
     3961 agaagaggag gaggaggacg aggaggagga agaagaggag gaagaagagg aagaggatga
     4021 agaggaggaa gaagaggaag aagaagaaga agaagaagaa aatattcaaa gctctccccc
     4081 aagattgacg aaaccacagt cagttgccat aaagagaaag aggccttttg tactaaagaa
     4141 gaaaaggggt cgtaaacgca ggaggatcaa cagcagtgta acaacagaga ccatttcaga
     4201 gacgacagaa gtactgaatg agccctttga caactcagat gaagagaggc caatgccaca
     4261 gctggagcct acctgtgaga ttgaagtgga ggaagatggc aggaagccag tcctgagaaa
     4321 agcattccag catcagcctg ggaagaaaag acaaacagag gaagaggaag gaaaagacaa
     4381 tcattgcttc aagaatgctg acccttgtag aaacaatatg aatgatgatt caagtaactt
     4441 gaaagaaggc agtaaagaca atcccgaacc tctaaagtgc aaacaagtgt ggccaaaagg
     4501 taagtatcaa ggttacaaga caggtttaag gagaccaata agctattatt cgtataggat
     4561 actttatacg aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg
     4621 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4681 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4741 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4801 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4861 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     4921 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4981 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5041 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5101 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5161 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     5221 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5281 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5341 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5401 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5461 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5521 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5581 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5641 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5701 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5761 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5821 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5881 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5941 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6001 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6061 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6121 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6181 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6241 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6301 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6361 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6421 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6481 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6541 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6601 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6661 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6721 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6781 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6841 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6901 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6961 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7021 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7081 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7141 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7201 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7261 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7321 tacggttcct ggccttttgc tggccttttg ctcacatgt
//