| 质粒名称: | pALB-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 55759 |
| 存储实验室: | Snorri Thorgeirsson |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4151 bp |
| 完整质粒大小: | 6550 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | albumin enhancer/promoter |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 2335 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Mouse Albumin Enhancer/Promoter |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SacI (未破坏) |
| 3' 克隆位点: | KpnI (未破坏) |
| 5' 测序引物 | pBR322ori-F |
| 3' 测序引物 | EGFP-N |
|
文献引用方法 |
|
| 材料和方法部分: | pALB-GFP was a gift from Snorri Thorgeirsson (Addgene plasmid # 55759 ; http://n2t.net/addgene:55759 ; RRID:Addgene_55759) |
| 参考文献部分: | Hepatic precursors derived from murine embryonic stem cells contribute to regeneration of injured liver. Heo J, Factor VM, Uren T, Takahama Y, Lee JS, Major M, Feinstone SM, Thorgeirsson SS. Hepatology. 2006 Dec;44(6):1478-86. 10.1002/hep.21441 PubMed 17133486 |
买家导航
pALB-GFP载体基本信息
pALB-GFP载体图谱质粒图谱和多克隆位点信息
pALB-GFP载体简介
pALB-GFP载体序列
LOCUS pALB-GFP 6550 bp ds-DNA circular SYN 02-OCT-2020
DEFINITION Fluorescent marker for hepatocytic lineage.
ACCESSION .
VERSION .
KEYWORDS pALB-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6550)
AUTHORS Heo J, Factor VM, Uren T, Takahama Y, Lee JS, Major M, Feinstone SM,
Thorgeirsson SS
TITLE Hepatic precursors derived from murine embryonic stem cells
contribute to regeneration of injured liver.
JOURNAL Hepatology. 2006 Dec;44(6):1478-86.
PUBMED 17133486
REFERENCE 2 (bases 1 to 6550)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Oct 2, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6550
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(2331..2919)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2411..2430)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
polyA_signal 3248..3295
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
primer_bind 3320..3339
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
CDS complement(3527..4321)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3638..3657)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 4248..4267
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
promoter complement(4356..4713)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4370..4505
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(4424..4443)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 4670..4690
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter complement(4715..4819)
/gene="bla"
/label=AmpR promoter
rep_origin 4846..5301
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4933..4952)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5143..5164
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 5308..5429
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5319..5338)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 5373..5392
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS complement(5552..6271)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(5597..5618)
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 5946..5965
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 6205..6226
/label=EGFP-N
/note="EGFP, reverse primer"
regulatory 6268..6277
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind 6316..6332
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 6317..6333
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 atctggagct gttcccctct aaccccaccc ccacccccat gcacaagact ttgtccattc
61 attaaagtta tgtaaaacag caaattttac ataagagctt aatctctttg tctcccattt
121 gagcatttca gtgtgggcct tggcatggaa gcatgcctgc aggtcgatcc caagctggag
181 aacgagttca agccaagctg caccactgct tttcacacac tcttcactct gcatcagctt
241 agtatttctt aagaaattaa aagatggcaa aacacatcta aactgtatta ataaagtgct
301 tctttcatat ttaatgtttt tccagataaa gaaaactatg atgaatgcct gcatgcttat
361 ctatgtttca tagatcagca agtagaatgt ataaaatgga agtgtcagta attctgctca
421 taattattgc tgcagattga attcacccct aagcaaatat acctctgaac atctgctcac
481 agtctgtatg ttctccagac acaatccaaa agacttatta tctgaaagat taatgtcaca
541 aagccagagc tttataatct cttataaaac atagattgta gccaggcagt ggtggcacat
601 gcttttaatc ctagcacttg caaggcagag gcaagcagat ctctgagttc aagaccaacc
661 tggtctacag agcaaggtcc aggacagcca aagctagaca gaaaaaactg tatctcaaaa
721 gaaaatagac aacaaattac attgttacag ctaaaattat cttatgttga aatttctgta
781 gctcaacttt ggaatatttt cattagaggg taatatttga ttatgatcac ttctaaaact
841 ttagaattta ttgttttata atctcttggt ttcagtactt acctaaaatt ttccaaccag
901 tcacccagct aaaacttaaa atatttaagt cctagaattc cagttagttt tgcaagtaac
961 tataaatggt attacagtga gaaatggagc atctgatgtc tactcacatg taaactttac
1021 acatatcaaa tagatgattg tctatggtct ttcttctttt ttagagtata tagagtatat
1081 agagatagat tcatccataa taagctcaat aaacaaatgt ttaaaaatga ttgttagata
1141 ttattgggta tataagtacc taattattaa aattgacttt tttataacat tgagataaat
1201 taaaattcat ttattaaaat aatatatatg aatttgaagg ggtttttttt gcaaaacaat
1261 ttcagcaagc aataccatga caaaagtgtg tattcaaatg gaatgggaaa cgaatgtcag
1321 taacttatgg tccccgtgta ctcattccca gacatgcctg attggtagct gtgacagctc
1381 cagcgtactt aacaccaaga ctttaaataa gctgccaaaa atgtgtaaga ctgccatttc
1441 attagtttta atttttatat ctataccctt tctacagcca catactaaac gtagacaagt
1501 tggccttttc ctattgcttt aaaggcagag gactgtattg atcagtccaa acttctttct
1561 gcatgtacat ggaaaactgg ccaaggcaaa cacgtccgga atgatggtat ttaagaacaa
1621 acattccctg gtatcagcaa gtacagtgcc ctgctgacag agcaggagac acaaagtacc
1681 atctcgtccc tatgttaagt agtgtcacct catgctcaag ggatactgag tggatgctgt
1741 aacgcaggtt attttctagg ctgtgaggat acaagaaaat gaaagtaatt aaagtagaac
1801 attgctctgt gctatgcttg cagaatgtgt agtgtagtct aggaacagag aggggaaggt
1861 tctaaatcaa aaaaaatcaa gctcatgcct aaggatgtgt gggttgccac ctctttagct
1921 acctatgcga tccaaacaac tataaaactt agaatttatt ttctctggat gaatttgtgc
1981 ttgtggagca atgttggtag ggggcagggt cagctggaaa agtggaatga gcaagcagaa
2041 aactgagaga agcagaagct taggaagatg ggtaatttcc aaaagtttca caaaagatca
2101 aatcaaagaa gtaagctcca ccttagaaaa aagtggaacg tcatgctaag gaagctagag
2161 cggccgccac cgcggtggag ctcgagatct gagtccggta gcgctagtaa taactaatgc
2221 atggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
2281 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
2341 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
2401 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
2461 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
2521 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
2581 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
2641 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
2701 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
2761 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
2821 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
2881 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
2941 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
3001 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
3061 agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt ggctgcgagc
3121 cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat
3181 tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg
3241 cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat tgccgtcata
3301 gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc cctagggtgg
3361 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa
3421 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc
3481 aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt
3541 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
3601 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
3661 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
3721 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
3781 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
3841 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
3901 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
3961 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
4021 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
4081 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
4141 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
4201 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
4261 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
4321 tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag gcctccaaaa
4381 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
4441 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
4501 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
4561 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
4621 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
4681 cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa tattattgaa
4741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
4801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
4861 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
4921 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
4981 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
5041 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
5101 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
5161 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
5221 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
5281 aaatattaac gcttacaatt tacgccttaa gatacattga tgagtttgga caaaccacaa
5341 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
5401 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
5461 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
5521 tggctgatta tgatctagag tcgcggccgc tttacttgta cagctcgtcc atgccgagag
5581 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
5641 ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
5701 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
5761 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
5821 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
5881 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
5941 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
6001 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
6061 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
6121 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
6181 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
6241 cggtgaacag ctcctcgccc ttgctcacca tggtggcgac cggtggatcc cgggcccgcg
6301 gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc
6361 ccgggggatc ccggggttga taggaaaggt gatctgtgtg cagaaagact cgctctaata
6421 tacttcttta accaataact gtagatcatt aaccatactt acctcgcatt tcattggttc
6481 ctaccccatt acaaaatcat accatctttg ccaaaaagtt gtttgactaa atcccttgcg
6541 tatgtttgcc
//
