| 质粒名称: | pC-HYG-YR |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 61765 |
| 存储实验室: | Ken Haynes |
|
载体骨架基本信息 |
|
| 载体名称: | pCAMBIA-0380 |
| 载体出品公司: | CAMBIA |
| 空载体大小: | 6812 bp |
| 完整质粒大小: | 10738 bp |
| 载体类型: | 农杆菌介导转化的三元载体细胞表达 |
| 筛选标记: | Hygromycin, URA3 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称1: | Hygromycin |
| 物种来源: | Synthetic |
| 插入大小: | 951 bp |
| 启动子: | trpC promoter |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI(未破坏) |
| 3’克隆位点: | HindIII (未破坏) |
| 5’测序引物 | LB-F1 (5'-gtggtgtaaacaaattgacgc-3') |
| 3’测序引物 | RB-F1 (5'-ggataaaccttttcacgccc-3') |
| 插入基因名称2: | micron origin or replication and URA3 gene for S. cerevisiae |
| 物种来源: | S. cerevisiae (budding yeast) |
| 插入大小: | 2487bp |
| 基因银行ID： | KC562906.1 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SacII (not destroyed) |
| 3’克隆位点: | SacII (not destroyed) |
| 5’测序引物 | ccgcggTTAGTTTTGCTGGCCGCATC |
| 3’测序引物 | ccgcggCGCTTCCACAAACATTGCTC |
|
文献引用方法 |
|
| 材料和方法部分: | pC-HYG-YR was a gift from Ken Haynes (Addgene plasmid # 61765 ; http://n2t.net/addgene:61765 ; RRID:Addgene_61765) |
| 参考文献部分: | Exploitation of sulfonylurea resistance marker and non-homologous end joining mutants for functional analysis in Zymoseptoria tritici. Sidhu YS, Cairns TC, Chaudhari YK, Usher J, Talbot NJ, Studholme DJ, Csukai M, Haynes K. Fungal Genet Biol. 2015 Jun;79:102-9. doi: 10.1016/j.fgb.2015.04.015. 10.1016/j.fgb.2015.04.015 PubMed 26092796 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pC-HYG-YR 10738 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Yeast recombinational cloning compatible Agrobacterium tumefaciens
ternary vector containing hygromycin selectable marker on transfer
DNA (TDNA)..
ACCESSION .
VERSION .
KEYWORDS pC-HYG-YR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10738)
AUTHORS Sidhu YS, Cairns TC, Chaudhari YK, Usher J, Talbot NJ, Studholme DJ,
Csukai M, Haynes K
TITLE Exploitation of sulfonylurea resistance marker and non-homologous
end joining mutants for functional analysis in Zymoseptoria tritici.
JOURNAL Fungal Genet Biol. 2015 Jun;79:102-9. doi:
10.1016/j.fgb.2015.04.015.
PUBMED 26092796
REFERENCE 2 (bases 1 to 10738)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10738
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1280..1909
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 2343..3410
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 3476..3670
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(3906..3928)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 4014..4154
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 4069..4088
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(4169..4186)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4340..4928)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4420..4439)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5015..5809)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
CDS complement(5996..6799)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(6800..7015)
/gene="S. cerevisiae URA3"
/label=URA3 promoter
misc_feature 8727..8751
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 9234..10259
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
CDS 10368..10385
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 10420..10672
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 10694..10718
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
ORIGIN
1 ttagaataac ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
61 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc
121 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac
181 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
241 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
301 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
361 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
421 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
481 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
541 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
601 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
661 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc
721 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
781 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
841 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
901 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
961 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
1021 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
1081 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct
1141 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
1201 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
1261 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
1321 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
1381 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
1441 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
1501 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
1561 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
1621 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
1681 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
1741 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
1801 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
1861 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
1921 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
1981 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
2041 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
2101 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
2161 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
2221 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
2281 ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
2341 acggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
2401 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
2461 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
2521 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
2581 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
2641 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
2701 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
2761 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
2821 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
2881 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
2941 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
3001 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
3061 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
3121 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
3181 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
3241 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
3301 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
3361 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
3421 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
3481 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
3541 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
3601 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
3661 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
3721 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
3781 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
3841 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
3901 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
3961 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
4021 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
4081 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
4141 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
4201 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
4261 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
4321 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
4381 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
4441 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
4501 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
4561 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
4621 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
4681 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
4741 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
4801 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
4861 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
4921 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
4981 cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat
5041 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
5101 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
5161 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
5221 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
5281 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
5341 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
5401 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
5461 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
5521 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
5581 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
5641 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
5701 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
5761 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
5821 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
5881 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
5941 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggttagt
6001 tttgctggcc gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct
6061 ctaccttagc atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc
6121 ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat
6181 ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt
6241 ctctttgagc aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac
6301 ccttagtata ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa
6361 ggcctctagg ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg
6421 ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg
6481 cagagtactg caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta
6541 aaaaattgta cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat
6601 cagtcaagat atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta
6661 actccagtaa ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt
6721 cgtgcatgat attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct
6781 tatatgtagc tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt
6841 gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca
6901 atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa
6961 aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaaagc
7021 tagcttatcg atgataagct gtcaaacatg agaattaatt ccacggacta tagactatac
7081 tatatactcc gtctactgta cgatacactt ccgctcaggt ccttgtcctt taacgaggcc
7141 ttaccactct tttgttactc tattgatcca gctcagcaaa ggcagtgtga tctaagattc
7201 tattcgcgat gtagtaaaac tagctagacc gagaaagaga ctagaaatgc aaaaggcact
7261 tctacaatgg ctgccatcat tattatccga tgtgacgctg cagcttctca atgatattcg
7321 aatacgcttt gaggagatac agcctaatat ccgacaaact gttttacaga tttacgatcg
7381 tacttgttac ccatcattga attttgaaca tccgaacctg ggagttttcc ctgaaacaga
7441 tagtatattt gaacctgtat aataatatat agtctagcgc tttacggaag acaatgtatg
7501 tatttcggtt cctggagaaa ctattgcatc tattgcatag gtaatcttgc acgtcgcatc
7561 cccggttcat tttctgcgtt tccatcttgc acttcaatag catatctttg ttaacgaagc
7621 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
7681 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg
7741 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgacgagagc gctaattttt
7801 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
7861 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
7921 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
7981 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
8041 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
8101 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
8161 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
8221 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
8281 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
8341 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
8401 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
8461 ttgagcaatg tttgtggaag cgccgcggtg atcacaggca gcaacgctct gtcatcgtta
8521 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
8581 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
8641 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
8701 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
8761 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta
8821 attcctaggc caccatgttg ggcccggcgc gccgaattcg agctcggtac ccaactgata
8881 ttgaaggagc attttttggg cttggctgga gctagtggag gtcaacaatg aatgcctatt
8941 ttggtttagt cgtccaggcg gtgagcacaa aatttgtgtc gtttgacaag atggttcatt
9001 taggcaactg gtcagatcag ccccacttgt agcagtagcg gcggcgctcg aagtgtgact
9061 cttattagca gacaggaacg aggacattat tatcatctgc tgcttggtgc acgataactt
9121 ggtgcgtttg tcaagcaagg taagtggacg acccggtcat accttcttaa gttcgccctt
9181 cctcccttta tttcagattc aatctgactt acctattcta cccaagcatc caaatgaaaa
9241 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
9301 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
9361 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
9421 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt
9481 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
9541 tgcctgaaac cgaactgccc gctgttctcc agccggtcgc ggaggccatg gatgcgatcg
9601 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
9661 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
9721 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
9781 tttgggccga ggactgcccc gaagtccggc acctcgtgca tgcggatttc ggctccaaca
9841 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
9901 gggattccca atacgaggtc gccaacatcc tcttctggag gccgtggttg gcttgtatgg
9961 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcgcctcc
10021 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
10081 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
10141 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
10201 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga
10261 gtagatgccg accgggaacc agttggggat cctctagagt cgacctgcag gcatgcaagc
10321 tttaagagga gtccaccatg gtagatctga ctagtgttaa cgctagccac caccaccacc
10381 accacgtgtg aattacaggt gaccagctcg aatttccccg atcgttcaaa catttggcaa
10441 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
10501 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
10561 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
10621 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta
10681 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgttta
//
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