pC-HYG-YR

LOCUS       pC-HYG-YR               10738 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Yeast recombinational cloning compatible Agrobacterium tumefaciens 
            ternary vector containing hygromycin selectable marker on transfer 
            DNA (TDNA)..
ACCESSION   .
VERSION     .
KEYWORDS    pC-HYG-YR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10738)
  AUTHORS   Sidhu YS, Cairns TC, Chaudhari YK, Usher J, Talbot NJ, Studholme DJ,
            Csukai M, Haynes K
  TITLE     Exploitation of sulfonylurea resistance marker and non-homologous 
            end joining mutants for functional analysis in Zymoseptoria tritici.
  JOURNAL   Fungal Genet Biol. 2015 Jun;79:102-9. doi: 
            10.1016/j.fgb.2015.04.015.
  PUBMED    26092796
REFERENCE   2  (bases 1 to 10738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10738
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1280..1909
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2343..3410
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3476..3670
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3906..3928)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4014..4154
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4069..4088
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4169..4186)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4340..4928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4420..4439)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5015..5809)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             complement(5996..6799)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6800..7015)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     misc_feature    8727..8751
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9234..10259
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             10368..10385
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      10420..10672
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10694..10718
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttagaataac ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
       61 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc
      121 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac
      181 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
      241 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
      301 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
      361 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
      421 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
      481 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
      541 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
      601 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
      661 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc
      721 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
      781 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
      841 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
      901 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
      961 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
     1021 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
     1081 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct
     1141 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
     1201 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
     1261 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
     1321 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
     1381 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
     1441 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
     1501 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
     1561 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
     1621 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
     1681 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
     1741 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
     1801 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
     1861 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
     1921 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
     1981 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
     2041 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
     2101 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
     2161 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
     2221 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
     2281 ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
     2341 acggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
     2401 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
     2461 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
     2521 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
     2581 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
     2641 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
     2701 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
     2761 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
     2821 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
     2881 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
     2941 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
     3001 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
     3061 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
     3121 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
     3181 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
     3241 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
     3301 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
     3361 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
     3421 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
     3481 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
     3541 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
     3601 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
     3661 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
     3721 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
     3781 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
     3841 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
     3901 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     3961 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     4021 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     4081 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     4141 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4201 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4261 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4321 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4381 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4441 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4501 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4561 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4621 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4681 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4741 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4801 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4861 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4921 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4981 cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat
     5041 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
     5101 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
     5161 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
     5221 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
     5281 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
     5341 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
     5401 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
     5461 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
     5521 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
     5581 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
     5641 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
     5701 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
     5761 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
     5821 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
     5881 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
     5941 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggttagt
     6001 tttgctggcc gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct
     6061 ctaccttagc atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc
     6121 ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat
     6181 ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt
     6241 ctctttgagc aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac
     6301 ccttagtata ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa
     6361 ggcctctagg ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg
     6421 ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg
     6481 cagagtactg caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta
     6541 aaaaattgta cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat
     6601 cagtcaagat atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta
     6661 actccagtaa ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt
     6721 cgtgcatgat attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct
     6781 tatatgtagc tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt
     6841 gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca
     6901 atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa
     6961 aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaaagc
     7021 tagcttatcg atgataagct gtcaaacatg agaattaatt ccacggacta tagactatac
     7081 tatatactcc gtctactgta cgatacactt ccgctcaggt ccttgtcctt taacgaggcc
     7141 ttaccactct tttgttactc tattgatcca gctcagcaaa ggcagtgtga tctaagattc
     7201 tattcgcgat gtagtaaaac tagctagacc gagaaagaga ctagaaatgc aaaaggcact
     7261 tctacaatgg ctgccatcat tattatccga tgtgacgctg cagcttctca atgatattcg
     7321 aatacgcttt gaggagatac agcctaatat ccgacaaact gttttacaga tttacgatcg
     7381 tacttgttac ccatcattga attttgaaca tccgaacctg ggagttttcc ctgaaacaga
     7441 tagtatattt gaacctgtat aataatatat agtctagcgc tttacggaag acaatgtatg
     7501 tatttcggtt cctggagaaa ctattgcatc tattgcatag gtaatcttgc acgtcgcatc
     7561 cccggttcat tttctgcgtt tccatcttgc acttcaatag catatctttg ttaacgaagc
     7621 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     7681 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg
     7741 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgacgagagc gctaattttt
     7801 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     7861 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     7921 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     7981 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     8041 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     8101 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     8161 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     8221 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     8281 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     8341 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     8401 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     8461 ttgagcaatg tttgtggaag cgccgcggtg atcacaggca gcaacgctct gtcatcgtta
     8521 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
     8581 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
     8641 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
     8701 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
     8761 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta
     8821 attcctaggc caccatgttg ggcccggcgc gccgaattcg agctcggtac ccaactgata
     8881 ttgaaggagc attttttggg cttggctgga gctagtggag gtcaacaatg aatgcctatt
     8941 ttggtttagt cgtccaggcg gtgagcacaa aatttgtgtc gtttgacaag atggttcatt
     9001 taggcaactg gtcagatcag ccccacttgt agcagtagcg gcggcgctcg aagtgtgact
     9061 cttattagca gacaggaacg aggacattat tatcatctgc tgcttggtgc acgataactt
     9121 ggtgcgtttg tcaagcaagg taagtggacg acccggtcat accttcttaa gttcgccctt
     9181 cctcccttta tttcagattc aatctgactt acctattcta cccaagcatc caaatgaaaa
     9241 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     9301 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     9361 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     9421 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt
     9481 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     9541 tgcctgaaac cgaactgccc gctgttctcc agccggtcgc ggaggccatg gatgcgatcg
     9601 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     9661 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     9721 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     9781 tttgggccga ggactgcccc gaagtccggc acctcgtgca tgcggatttc ggctccaaca
     9841 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     9901 gggattccca atacgaggtc gccaacatcc tcttctggag gccgtggttg gcttgtatgg
     9961 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcgcctcc
    10021 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
    10081 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
    10141 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
    10201 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga
    10261 gtagatgccg accgggaacc agttggggat cctctagagt cgacctgcag gcatgcaagc
    10321 tttaagagga gtccaccatg gtagatctga ctagtgttaa cgctagccac caccaccacc
    10381 accacgtgtg aattacaggt gaccagctcg aatttccccg atcgttcaaa catttggcaa
    10441 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
    10501 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
    10561 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
    10621 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta
    10681 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgttta
//