| 质粒名称: | pAAVLINK-EF1a-5'-IGF1R |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001644 |
| 产品用途: | 5' AAVLINK plasmid for IGF1R expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | IGF1R |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-5'-IGF1R
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-5'-IGF1R载体质粒基本信息
pAAVLINK-EF1a-5'-IGF1R质粒图谱载体图谱和pAAVLINK-EF1a-5'-IGF1R载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-IGF1R质粒载体简介
pAAVLINK-EF1a-5'-IGF1R质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-IGF1R 5661 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5661)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5661
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 713..2801
/codon_start=1
/label=IGF1R
/translation="MKSGSGGGSPTSLWGLLFLSAALSLWPTSGEICGPGIDIRNDYQQ
LKRLENCTVIEGYLHILLISKAEDYRSYRFPKLTVITEYLLLFRVAGLESLGDLFPNLT
VIRGWKLFYNYALVIFEMTNLKDIGLYNLRNITRGAIRIEKNADLCYLSTVDWSLILDA
VSNNYIVGNKPPKECGDLCPGTMEEKPMCEKTTINNEYNYRCWTTNRCQKMCPSTCGKR
ACTENNECCHPECLGSCSAPDNDTACVACRHYYYAGVCVPACPPNTYRFEGWRCVDRDF
CANILSAESSDSEGFVIHDGECMQECPSGFIRNGSQSMYCIPCEGPCPKVCEEEKKTKT
IDSVTSAQMLQGCTIFKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSL
SFLKNLRLILGEEQLEGNYSFYVLDNQNLQQLWDWDHRNLTIKAGKMYFAFNPKLCVSE
IYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLHFTSTTTSKNRIIITWHRYRPPDY
RDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKDVEPGILLHGLKPWTQ
YAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPS
LPNGNLSYYIVRWQRQPQDGYLYRHNYCSKDKIPIRKYADGTIDIEEVTENPKTEVCGG
EK"
misc_feature 730..735
/label=SDS
misc_feature 2169..2174
/label=SDS
misc_feature 2754..2759
/label=SDS
intron 2802..2842
/label=Splicing Donor
misc_feature 2846..2879
/label=lox2272-JT1571
repeat_region 2924..3064
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2924..3053
/label=AAV2 ITR
rep_origin 3139..3594
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3876..3980
/gene="bla"
/label=AmpR promoter
CDS 3981..4841
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5012..5600
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgaagtc
721 tggctccgga ggagggtccc cgacctcgct gtgggggctc ctgtttctct ccgccgcgct
781 ctcgctctgg ccgacgagtg gagaaatctg cgggccaggc atcgacatcc gcaacgacta
841 tcagcagctg aagcgcctgg agaactgcac ggtgatcgag ggctacctcc acatcctgct
901 catctccaag gccgaggact accgcagcta ccgcttcccc aagctcacgg tcattaccga
961 gtacttgctg ctgttccgag tggctggcct cgagagcctc ggagacctct tccccaacct
1021 cacggtcatc cgcggctgga aactcttcta caactacgcc ctggtcatct tcgagatgac
1081 caatctcaag gatattgggc tttacaacct gaggaacatt actcgggggg ccatcaggat
1141 tgagaaaaat gctgacctct gttacctctc cactgtggac tggtccctga tcctggatgc
1201 ggtgtccaat aactacattg tggggaataa gcccccaaag gaatgtgggg acctgtgtcc
1261 agggaccatg gaggagaagc cgatgtgtga gaagaccacc atcaacaatg agtacaacta
1321 ccgctgctgg accacaaacc gctgccagaa aatgtgccca agcacgtgtg ggaagcgggc
1381 gtgcaccgag aacaatgagt gctgccaccc cgagtgcctg ggcagctgca gcgcgcctga
1441 caacgacacg gcctgtgtag cttgccgcca ctactactat gccggtgtct gtgtgcctgc
1501 ctgcccgccc aacacctaca ggtttgaggg ctggcgctgt gtggaccgtg acttctgcgc
1561 caacatcctc agcgccgaga gcagcgactc cgaggggttt gtgatccacg acggcgagtg
1621 catgcaggag tgcccctcgg gcttcatccg caacggcagc cagagcatgt actgcatccc
1681 ttgtgaaggt ccttgcccga aggtctgtga ggaagaaaag aaaacaaaga ccattgattc
1741 tgttacttct gctcagatgc tccaaggatg caccatcttc aagggcaatt tgctcattaa
1801 catccgacgg gggaataaca ttgcttcaga gctggagaac ttcatggggc tcatcgaggt
1861 ggtgacgggc tacgtgaaga tccgccattc tcatgccttg gtctccttgt ccttcctaaa
1921 aaaccttcgc ctcatcctag gagaggagca gctagaaggg aattactcct tctacgtcct
1981 cgacaaccag aacttgcagc aactgtggga ctgggaccac cgcaacctga ccatcaaagc
2041 agggaaaatg tactttgctt tcaatcccaa attatgtgtt tccgaaattt accgcatgga
2101 ggaagtgacg gggactaaag ggcgccaaag caaaggggac ataaacacca ggaacaacgg
2161 ggagagagcc tcctgtgaaa gtgacgtcct gcatttcacc tccaccacca cgtcgaagaa
2221 tcgcatcatc ataacctggc accggtaccg gccccctgac tacagggatc tcatcagctt
2281 caccgtttac tacaaggaag caccctttaa gaatgtcaca gagtatgatg ggcaggatgc
2341 ctgcggctcc aacagctgga acatggtgga cgtggacctc ccgcccaaca aggacgtgga
2401 gcccggcatc ttactacatg ggctgaagcc ctggactcag tacgccgttt acgtcaaggc
2461 tgtgaccctc accatggtgg agaacgacca tatccgtggg gccaagagtg agatcttgta
2521 cattcgcacc aatgcttcag ttccttccat tcccttggac gttctttcag catcgaactc
2581 ctcttctcag ttaatcgtga agtggaaccc tccctctctg cccaacggca acctgagtta
2641 ctacattgtg cgctggcagc ggcagcctca ggacggctac ctttaccggc acaattactg
2701 ctccaaagac aaaatcccca tcaggaagta tgccgacggc accatcgaca ttgaggaggt
2761 cacagagaac cccaagactg aggtgtgtgg tggggagaaa ggtaagtatc aaggttacaa
2821 gacaggttta aggagaccaa taagctatta ttcgtatagg atactttata cgaagttatt
2881 aagttatgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg
2941 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
3001 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
3061 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
3121 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
3181 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
3241 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
3301 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
3361 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
3421 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
3481 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
3541 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
3601 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
3661 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
3721 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
3781 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
3841 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
3901 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
3961 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
4021 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
4081 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
4141 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
4201 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
4261 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
4321 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
4381 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
4441 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
4501 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
4561 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
4621 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
4681 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
4741 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
4801 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
4861 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
4921 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
4981 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
5041 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
5101 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
5161 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
5221 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
5281 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
5341 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
5401 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
5461 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
5521 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
5581 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
5641 gctggccttt tgctcacatg t
//
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