| 质粒名称: | pSLCAR-CD19-CD3z |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 135993 |
| 存储实验室: | Scott McComb |
|
载体骨架基本信息 |
|
| 载体名称: | pSL |
| 载体出品公司: | Heavily modified from LentiCRISPR v1 backbone |
| 载体抗性: | 氨苄青霉素 |
| 载体修饰: | 使用BpiI单锅限制性连接将CAR的抗原结合域从CD19交换到所需的特异性 |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | FMC63-3z-P2A-EGFP,CD19(FMC63)-CAR, no costim., CD3z stim domains, P2A-GFP marker,Contains: FMC63-scFV, Hinge domain, CD28-transmembrane domain, CD3z-ITD |
| 物种来源: | H. sapiens (human) |
| 启动子 | EF1a-short |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BpiI（在克隆过程中被破坏） |
| 3' 克隆位点: | BpiI（在克隆过程中被破坏） |
| 5' 测序引物 | CGGGTTTGCCGCCA |
| 3' 测序引物 | caggagccTGCTCCTCTTACTcc |
|
文献引用方法 |
|
| 材料和方法部分: | pSLCAR-CD19-CD3z was a gift from Scott McComb (Addgene plasmid # 135993 ; http://n2t.net/addgene:135993 ; RRID:Addgene_135993) |
| 参考文献部分: | A High-Throughput Method for Characterizing Novel Chimeric Antigen Receptors in Jurkat Cells. Bloemberg D, Nguyen T, MacLean S, Zafer A, Gadoury C, Gurnani K, Chattopadhyay A, Ash J, Lippens J, Harcus D, Page M, Fortin A, Pon RA, Gilbert R, Marcil A, Weeratna RD, McComb S. Mol Ther Methods Clin Dev. 2020 Jan 31;16:238-254. doi: 10.1016/j.omtm.2020.01.012. eCollection 2020 Mar 13. 10.1016/j.omtm.2020.01.012 PubMed 32083149 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSLCAR-CD19-CD3z 8746 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Modular CD3z alone CAR backbone, For Transient Expression or
Lentiviral Production.
ACCESSION .
VERSION .
KEYWORDS pSLCAR-CD19-CD3z
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8746)
AUTHORS Bloemberg D, Nguyen T, MacLean S, Zafer A, Gadoury C, Gurnani K,
Chattopadhyay A, Ash J, Lippens J, Harcus D, Page M, Fortin A, Pon
RA, Gilbert R, Marcil A, Weeratna RD, McComb S
TITLE A High-Throughput Method for Characterizing Novel Chimeric Antigen
Receptors in Jurkat Cells.
JOURNAL Mol Ther Methods Clin Dev. 2020 Jan 31;16:238-254. doi:
10.1016/j.omtm.2020.01.012. eCollection 2020 Mar 13.
PUBMED 32083149
REFERENCE 2 (bases 1 to 8746)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8746
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 382..580
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 598..778
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 825..950
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1443..1676
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1861..1905
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2128..2245
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2271..2482
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 2571..2636
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 3981..4037
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 4038..4754
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
CDS 4038..4751
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(4080..4101)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4341..4360)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4688..4709
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 4769..5357
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4822..4842)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5240..5251)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5429..5662
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5740..5861
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5777..5796)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5831..5850
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5901..6036
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5963..5982
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6056..6075)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6057..6075)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6085..6102)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6085..6101)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6094..6116)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6243..6698
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6330..6349)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6540..6561
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6724..6828
/gene="bla"
/label=AmpR promoter
CDS 6829..7689
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7047..7066)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7860..8448
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8349..8368
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(8556..8575)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
2041 ttatcgtttc agacccacct cccaaccccg aggggacccc gggtttatta cagggacagc
2101 agagatccac tttggcgccg gctcgagttt taaaagaaaa ggggggattg gggggtacag
2161 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
2221 acaaattaca aaaattcaaa atttttcgag tggctccggt gcccgtcagt gggcagagcg
2281 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
2341 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
2401 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
2461 cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc cgccaccatg ctcaggctgc
2521 tcttggctct caacttattc ccttcaattc aagtaacagg agggtcttcg gactacaagg
2581 atcatgacgg agactataag gatcacgata ttgattacaa agatgacgac gacaaagaca
2641 tccagatgac acagactaca tcctccctgt ctgcctctct gggagacaga gtcaccatca
2701 gttgcagggc aagtcaggac atctctaagt atttgaattg gtatcagcag aaaccagatg
2761 gaactgttaa actcctgatc taccatacat caagattaca ctcaggagtc ccatcaaggt
2821 tcagtggcag tgggtctgga acagattatt ctctcaccat tagcaacctg gagcaagaag
2881 atattgccac ttacttttgc caacagggta atacgcttcc gtacacgttc ggagggggga
2941 ctaagttgga aataacaggc tccacctctg gatccggcaa gcccggatct ggcgagggat
3001 ccaccaaggg cgaggtgaaa ctgcaggagt caggacctgg cctggtggcg ccctcacaga
3061 gcctgtccgt cacatgcact gtctcagggg tctcattacc cgactatggt gtaagctgga
3121 ttcgccagcc tccacgaaag ggtctggagt ggctgggagt aatatggggt agtgaaacca
3181 catactataa ttcagctctc aaatccagac tgaccatcat caaggacaac tccaagagcc
3241 aagttttctt aaaaatgaac agtctgcaaa ctgatgacac agccatttac tactgtgcca
3301 aacattatta ctacggtggt agctatgcta tggactactg gggtcaagga acctcagtca
3361 ccgtctcctc agcggccgca ggtaccacca caacgcccgc tcctcggcca ccgacgccag
3421 cgccaactat tgcgagtcag cctctcagtc tgcgacctga ggcttgtcga ccagcagccg
3481 gaggcgcagt gcacacgagg gggctggact tcgcctgtga tagaagacct ccttctaagc
3541 ccttttgggt gctggtggtg gttggtggag tcctggcttg ctatagcttg ctagtaacag
3601 tggcctttat tattttctgg gtgagggcta gcctgagagt gaagttcagc aggagcgcag
3661 acgcccccgc gtaccagcag ggccagaacc agctctataa cgagctcaat ctaggacgaa
3721 gagaggagta cgatgttttg gacaagagac gtggccggga ccctgagatg gggggaaagc
3781 cgcagagaag gaagaaccct caggaaggcc tgtacaatga actgcagaaa gataagatgg
3841 cggaggccta cagtgagatt gggatgaaag gcgagcgccg gaggggcaag gggcacgatg
3901 gcctttacca gggtctcagt acagccacca aggacaccta cgacgccctt cacatgcagg
3961 ccctgccccc tcgcgctagc gccacgaact tctctctgtt aaagcaagca ggcgacgtgg
4021 aagaaaaccc cggtcccgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
4081 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
4141 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
4201 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
4261 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
4321 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
4381 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
4441 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
4501 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
4561 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
4621 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
4681 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
4741 agctgtacaa gtaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
4801 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
4861 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
4921 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
4981 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
5041 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
5101 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
5161 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
5221 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
5281 gccggctctg cggcctcttc cgcgtctttg ccttcgccct cagacgagtc ggatctccct
5341 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
5401 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa
5461 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
5521 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
5581 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
5641 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
5701 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
5761 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
5821 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
5881 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
5941 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
6001 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
6061 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
6121 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
6181 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
6241 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
6301 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
6361 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
6421 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
6481 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
6541 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
6601 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
6661 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
6721 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
6781 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
6841 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
6901 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
6961 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
7021 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
7081 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
7141 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
7201 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
7261 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
7321 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
7381 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
7441 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
7501 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
7561 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
7621 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
7681 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
7741 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
7801 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
7861 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
7921 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
7981 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
8041 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
8101 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
8161 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
8221 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
8281 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
8341 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
8401 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
8461 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtg tcgacggatc
8521 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
8581 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
8641 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
8701 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
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