| 质粒名称: | pISH-Six1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 74358 |
| 存储实验室: | Peter Mombaerts |
|
载体骨架基本信息 |
|
| 载体名称: | pGEM-T easy |
| 载体出品公司: | Promega |
| 空载体大小: | 3015 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | sine oculis-related homeobox 1,Six1 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 850 bp |
| 基因突变: | -- |
| GenBank编号 | NM_009189.3 |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | T7 |
| 3’测序引物 | SP6 |
|
文献引用方法 |
|
| 材料和方法部分: | pISH-Six1 was a gift from Peter Mombaerts (Addgene plasmid # 74358 ; http://n2t.net/addgene:74358 ; RRID:Addgene_74358) |
| 参考文献部分: | Expression of homeobox genes in the mouse olfactory epithelium. Parrilla M, Chang I, Degl'Innocenti A, Omura M. J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. Epub 2016 Jun 19. 10.1002/cne.24051 PubMed 27243442 |
买家导航
pISHSix1载体基本信息
pISHSix1载体图谱质粒图谱和多克隆位点信息
pISHSix1载体简介
pISHSix1载体序列
LOCUS pISH-Six1 3868 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Use to synthesize riboprobes for in situ hybridization (ISH) probe
for Six1. Not for gene expression..
ACCESSION .
VERSION .
KEYWORDS pISH-Six1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3868)
AUTHORS Parrilla M, Chang I, Degl'Innocenti A, Omura M
TITLE Expression of homeobox genes in the mouse olfactory epithelium.
JOURNAL J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051.
Epub 2016 Jun 19.
PUBMED 27243442
REFERENCE 2 (bases 1 to 3868)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3868
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 33..42
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 37..46
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
promoter complement(993..1011)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(994..1011)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(1029..1045)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1029..1045)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1042..1064)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1053..1069
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1077..1107)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1122..1143
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1260..1277)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1431..2019)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1511..1530)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2190..3050)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2813..2832
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3051..3155)
/gene="bla"
/label=AmpR promoter
rep_origin complement(3233..3688)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3370..3391)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3582..3601
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3814..3836
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 3828..3845
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 3829..3845
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3852..3
/label=T7
/note="T7 promoter, forward primer"
promoter 3852..2
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
61 tctcggtcct tctgctccag agcaatatgg gccacgccag gagctcaaac tattctcttc
121 caggcctcac agcctcccag cccagccacg gtctgcaagc ccatcagcac cagctccagg
181 actctctgct gggcccactc acctccagtt tggtggactt gggttcctaa gtggggagat
241 attggggcct tgaagggatg gccggagcag caaccaaccg cagcaatcta ggggacactt
301 gtacatagaa gccagggaca attctgcagc ttgtttctgg ggttctttgc gcagaaagga
361 accacaggct ttcacaattg tctttttaaa aattacaacc aacagcggtc tcagttgagc
421 atcctctccc tcctctccaa ctcttaatac ccttgcattt cccctcccac taaaaggtca
481 ggaaataaca aaaacagcca ggcttggttc tgggcaaccc aggcatcctt tacagtagtc
541 ccttattcca cctcttcttc ctcctcctcc tcgtcttctt ttaatgaaca gaaattacaa
601 atcagctgga ttgtaattat ttttttaatt tatttatgga gaaacggttt agggaagagg
661 aaaaggaaat gaagagagat caaaataatg aaaacaagaa accttcagtg tgaggggaac
721 tggctgcctt cttaagacaa ataggaaaac tataatcaca gctgtttttt gatgggaaga
781 ttaagtttac atttttcata tttagtgtta aataatttta tgtagattaa aataaaagag
841 cagtattagc aagggaaaca agtgcctaaa tgtcttaatg ctctctatgg gaggcggtta
901 gggtagcagc gaaatcacta gtgaattcgc ggccgcctgc aggtcgacca tatgggagag
961 ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa tagcttggcg
1021 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
1081 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
1141 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
1201 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
1261 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
1321 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
1381 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
1441 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
1501 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
1561 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
1621 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
1681 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
1741 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
1801 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
1861 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
1921 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
1981 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
2041 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
2101 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
2161 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
2221 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
2281 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
2341 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
2401 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
2461 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
2521 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
2581 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
2641 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
2701 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
2761 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
2821 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
2881 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
2941 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
3001 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
3061 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
3121 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
3181 gatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
3241 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
3301 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
3361 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
3421 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
3481 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
3541 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
3601 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
3661 gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg
3721 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
3781 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
3841 ccagtgaatt gtaatacgac tcactata
//
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