pNTAP-C

价格:1500元

联系方式:I47-825O-882O

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pNTAPC载体基本信息

出品公司: Agilent Technologies
载体名称: pNTAP-C, pNTAPC
质粒类型: 哺乳细胞表达系统载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4532bp
5' 测序引物及序列: CMV-F: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: SV40pA-R: GAAATTTGTGATGCTATTGC
载体标签: streptavidin-binding peptides ;calmodulin-binding peptides 
载体抗性: 卡那霉素
筛选标记: Neomycin_1.html' target='_blank'>新霉素
备注: 能够表达N端含有能结合链霉亲和素的多肽片段,以及N端结合钙调蛋白结合多肽片段的载体.
产品目录号: --
稳定性: 诱导表达
组成型: 非组成型
病毒/非病毒: 非病毒

pNTAPC载体质粒图谱和多克隆位点信息

pNTAP-C载体图谱
pNTAP-C多克隆位点

pNTAP-C载体特征

pNTAPC载体简介

pNTAPC载体序列

LOCUS       pNTAP-C           4532 bp ds-DNA    circular SYN 28-十一月-2013
DEFINITION  Vector for expressing proteins N-terminally tagged with calmodulin- 
            and streptavidin-binding peptides.?For other reading frames, use 
            pNTAP-A or pNTAP-B.
ACCESSION   .
VERSION     .
KEYWORDS    pNTAP-C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        66..370
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        620..638
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             679..681
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             682..759
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /note="CBP"
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             778..891
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /note="SBP"
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     misc_feature    915..988
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    996..1006
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     promoter        complement(1034..1052)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1326..1447
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1454..1909)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1936..2038
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        2042..2399
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2434..3228
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
     misc_feature    3460..3507
                     /note="HSV TK poly(A) signal"
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      3836..4424
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccaccat gaagcgacga tggaaaaaga atttcatagc cgtctcagca
      721 gccaaccgct ttaagaaaat ctcatcctcc ggggcacttg gaagcggtag cggtaccatg
      781 gacgagaaga ccaccggctg gcggggcggc cacgtggtgg agggcctggc cggcgagctg
      841 gagcagctgc gggccaggct ggagcaccac cctcagggcc agcgggagcc ctccggcggc
      901 tgcaagctgg gcttgcccgg gcggatcccc cgggctgcag gaattcgata tcaagcttat
      961 cgataccgtc gacctcgagg gggggcccgg taccttaatt aattaaggta ccaggtaagt
     1021 gtacccaatt cgccctatag tgagtcgtat tacaattcac tcgatcgccc ttcccaacag
     1081 ttgcgcagcc tgaatggcga atggagatcc aatttttaag tgtataatgt gttaaactac
     1141 tgattctaat tgtttgtgta ttttagattc acagtcccaa ggctcatttc aggcccctca
     1201 gtcctcacag tctgttcatg atcataatca gccataccac atttgtagag gttttacttg
     1261 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     1321 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1381 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1441 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     1501 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     1561 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     1621 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     1681 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     1741 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     1801 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     1861 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     1921 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1981 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagaat
     2041 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     2101 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     2161 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2221 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     2281 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     2341 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     2401 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     2461 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     2521 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     2581 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     2641 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     2701 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     2761 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     2821 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     2881 gccggtcttg tcgatcagga tgatctggac gaagaacatc aggggctcgc gccagccgaa
     2941 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     3001 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     3061 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     3121 gaagaacttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     3181 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     3241 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     3301 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     3361 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     3421 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     3481 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     3541 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     3601 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     3661 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     3721 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3781 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3841 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3901 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3961 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     4021 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4081 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4141 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4201 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4261 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4321 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4381 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4441 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4501 cccctgattc tgtggataac cgtattaccg cc
//

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