pLVX-EF1α-IRES-ZsGreen1

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-EF1α-IRES-ZsGreen1, pLVX-EF1a-IRES-ZsGreen1, pLVX-EF1alpha-IRES-ZsGreen1
质粒类型: 哺乳动物细胞慢病毒表达载体
高拷贝/低拷贝: 高拷贝
启动子: EF1α/ EF1a
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8889 bp
5' 测序引物及序列: EF1a Forward: TCAAGCCTCAGACAGTGGTTC
3' 测序引物及序列: IRES-R: CCTCACATTGCCAAAAGACG
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: ZsGreen1
备注: 克隆宿主菌株 Stbl3
产品目录号: 631982
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-EF1α-IRES-ZsGreen1载体图谱



pLVX-EF1α-IRES-ZsGreen1多克隆位点

pLVX-EF1α-IRES-ZsGreen1载体特征

载体简介

载体序列

LOCUS       pLVX-EF1a-IRES-ZsGreen1   8889 bp    DNA   circular            11-FEB-2010
COMMENT     Created by Clontech Laboratories Inc.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     Cat. No. 631982
FEATURES             Location/Qualifiers
     CDS             4149..4844
                     /label=ZsGreen1
     misc_feature    3574..4148
                     /label=IRES
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
     misc_feature    4858..5449
                     /label=WPRE
     rep_origin      complement(6757..7430)
                     /label=pUC\origin
     CDS             complement(7575..8571)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             5652..6288
                     /label=3'\LTR
     misc_feature    3535..3572
                     /label=MCS
     promoter        2186..3519
                     /label=EF1a\Promoter
BASE COUNT     2190 a      2213 c      2366 g      2120 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 
     3541 ctcgagacta gttctagagc ggccgcggat cccgcccctc tccctccccc ccccctaacg 
     3601 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca 
     3661 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga 
     3721 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga 
     3781 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca 
     3841 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag 
     3901 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa 
     3961 gagtcaaatg gctcacctca agcgtattca acaaggggct gaaggatgcc cagaaggtac 
     4021 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga 
     4081 ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg 
     4141 atgataatat ggcccagtcc aagcacggcc tgaccaagga gatgaccatg aagtaccgca 
     4201 tggagggctg cgtggacggc cacaagttcg tgatcaccgg cgagggcatc ggctacccct 
     4261 tcaagggcaa gcaggccatc aacctgtgcg tggtggaggg cggccccttg cccttcgccg 
     4321 aggacatctt gtccgccgcc ttcatgtacg gcaaccgcgt gttcaccgag tacccccagg 
     4381 acatcgtcga ctacttcaag aactcctgcc ccgccggcta cacctgggac cgctccttcc 
     4441 tgttcgagga cggcgccgtg tgcatctgca acgccgacat caccgtgagc gtggaggaga 
     4501 actgcatgta ccacgagtcc aagttctacg gcgtgaactt ccccgccgac ggccccgtga 
     4561 tgaagaagat gaccgacaac tgggagccct cctgcgagaa gatcatcccc gtgcccaagc 
     4621 agggcatctt gaagggcgac gtgagcatgt acctgctgct gaaggacggt ggccgcttgc 
     4681 gctgccagtt cgacaccgtg tacaaggcca agtccgtgcc ccgcaagatg cccgactggc 
     4741 acttcatcca gcacaagctg acccgcgagg accgcagcga cgccaagaac cagaagtggc 
     4801 acctgaccga gcacgccatc gcctccggct ccgccttgcc ctgaacgcgt ctggaacaat 
     4861 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 
     4921 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 
     4981 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 
     5041 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 
     5101 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 
     5161 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 
     5221 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 
     5281 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 
     5341 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 
     5401 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct 
     5461 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg 
     5521 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc 
     5581 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 
     5641 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt 
     5701 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg 
     5761 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg 
     5821 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga 
     5881 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc 
     5941 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca 
     6001 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc 
     6061 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta 
     6121 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 
     6181 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 
     6241 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 
     6301 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct 
     6361 tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag 
     6421 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 
     6481 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 
     6541 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 
     6601 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 
     6661 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
     6721 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
     6781 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
     6841 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
     6901 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
     6961 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 
     7021 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
     7081 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
     7141 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
     7201 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 
     7261 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
     7321 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
     7381 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
     7441 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
     7501 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
     7561 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
     7621 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
     7681 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
     7741 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
     7801 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
     7861 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 
     7921 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
     7981 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
     8041 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
     8101 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
     8161 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 
     8221 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
     8281 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
     8341 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
     8401 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
     8461 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
     8521 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg 
     8581 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     8641 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     8701 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact 
     8761 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg 
     8821 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa 
     8881 cttagtagt 
//

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