| 质粒名称: | pDONR223_PER1_WT |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 82152 |
| 存储实验室: | Jesse Boehm, William Hahn, David Root |
|
载体骨架基本信息 |
|
| 载体名称: | pDONR223 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 2790 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | Gateway Entry vector |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PER1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | WT |
| GenBank编号 | NM_002616.2 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13pUC-fwd |
| 3' 测序引物 | M13-Rev |
|
文献引用方法 |
|
| 材料和方法部分: | pDONR223_PER1_WT was a gift from Jesse Boehm & William Hahn & David Root (Addgene plasmid # 82152 ; http://n2t.net/addgene:82152 ; RRID:Addgene_82152) |
| 参考文献部分: | Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC. Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. 10.1158/2159-8290.CD-16-0160 PubMed 27147599 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDONR223_PER1_WT 6666 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Gateway Donor vector containing PER1 , part of the Target
Accelerator Plasmid Collection..
ACCESSION .
VERSION .
KEYWORDS pDONR223_PER1_WT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6666)
AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG,
Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
WC
TITLE Systematic Functional Interrogation of Rare Cancer Variants
Identifies Oncogenic Alleles.
JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi:
10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
PUBMED 27147599
REFERENCE 2 (bases 1 to 6666)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6666
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 43..65
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 57..74
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 58..74
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 90..189
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
protein_bind complement(4066..4165)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(4182..4201)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(4183..4201)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4206..4222)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4206..4222)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 4653..5444
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 5537..6125
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6026..6045
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6279..6296
/label=L4440
/note="L4440 vector, forward primer"
terminator 6455..6482
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 6574..6660
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
181 aagttggcac catgagtggc cccctagaag gggctgatgg gggaggggac cccaggcctg
241 gggaatcatt ttgtcctggg ggcgtcccat cccctgggcc cccacagcac cggccttgcc
301 caggccccag cctggccgat gacaccgatg ccaacagcaa tggttcaagt ggcaatgagt
361 ccaacgggca tgagtctaga ggcgcatctc agcggagctc acacagctcc tcctcaggca
421 acggcaagga ctcagccctg ctggagacca ctgagagcag caagagcaca aactctcaga
481 gcccatcccc acccagcagt tccattgcct acagcctcct gagtgccagc tcagagcagg
541 acaacccgtc caccagtggc tgcagcagtg aacagtcagc ccgggcaagg actcagaagg
601 aactcatgac agcacttcga gagctcaagc ttcgactgcc gccagagcgc cggggcaagg
661 gccgctctgg gaccctggcc acgctgcagt acgcactggc ctgtgtcaag caggtgcagg
721 ccaaccagga atactaccag cagtggagcc tggaggaggg cgagccttgc tccatggaca
781 tgtccaccta taccctggag gagctggagc acatcacgtc tgagtacaca cttcagaacc
841 aggatacctt ctcagtggct gtctccttcc tgacgggccg aatcgtctac atttcggagc
901 aggcagccgt cctgctgcgt tgcaagcggg acgtgttccg gggtacccgc ttctctgagc
961 tcctggctcc ccaggatgtg ggagtcttct atggttccac tgctccatct cgcctgccca
1021 cctggggcac aggggcctca gcaggttcag gcctcaggga ctttacccag gagaagtccg
1081 tcttctgccg tatcagagga ggtcctgacc gggatccagg gcctcggtac cagccattcc
1141 gcctaacccc gtatgtgacc aagatccggg tctcagatgg ggcccctgca cagccgtgct
1201 gcctgctgat tgcagagcgc atccattcgg gttacgaagc tccccggata ccccctgaca
1261 agaggatttt cactacgcgg cacacaccca gctgcctctt ccaggatgtg gatgaaaggg
1321 ctgcccccct gctgggctac ctgccccagg acctcctggg ggccccagtg ctcctgttcc
1381 tgcatcctga ggaccgaccc ctcatgctgg ctatccacaa gaagattctg cagttggcgg
1441 gccagccctt tgaccactcc cctatccgct tctgtgcccg caacggggag tatgtcacca
1501 tggacaccag ctgggctggc tttgtgcacc cctggagccg caaggtagcc ttcgtgttgg
1561 gccgccacaa agtacgcacg gcccccctga atgaggacgt gttcactccc ccggccccca
1621 gcccagctcc ctccctggac actgatatcc aggagctgtc agagcagatc caccggctgc
1681 tgctgcagcc cgtccacagc cccagcccca cgggactctg tggagtcggc gccgtgacat
1741 ccccaggccc tctccacagc cctgggtcct ccagtgatag caacgggggt gatgcagagg
1801 ggcctgggcc tcctgcgcca gtgactttcc agcagatctg taaggatgtg catctggtga
1861 agcaccaggg ccagcagctt tttattgagt ctcgggcccg gcctcagtcc cggccccgcc
1921 tccctgctac aggcacgttc aaggccaagg cccttccctg ccaatcccca gacccagagc
1981 tggaggcggg ttctgctccc gtccaggccc cactagcctt ggtccctgag gaggccgaga
2041 ggaaagaagc ctccagctgc tcctaccagc agatcaactg cctggacagc atcctcaggt
2101 acctggagag ctgcaacctc cccagcacca ctaagcgtaa atgtgcctcc tcctcctcct
2161 ataccacctc ctcagcctct gacgacgaca ggcagaggac aggtccagtc tctgtgggga
2221 ccaagaaaga tccgccgtca gcagcgctgt ctggggaggg ggccacccca cggaaggagc
2281 cagtggtggg aggcaccctg agcccgctcg ccctggccaa taaggcggag agtgtggtgt
2341 ccgtcaccag tcagtgtagc ttcagctcca ccatcgtcca tgtgggagac aagaagcccc
2401 cggagtcgga catcatcatg atggaggacc tgcctggtct agccccaggc ccagccccca
2461 gcccagcccc cagccccaca gtagcccctg acccagcccc agacgcctac cgtccagtgg
2521 ggctgaccaa ggccgtgctg tccctgcaca cgcagaagga agagcaagcc ttcctcagcc
2581 gcttccgaga cctgggcagg ctgcgtggac tcgacagctc ttccacagct ccctcagccc
2641 ttggcgagcg aggctgccac cacggccccg cacccccaag ccgccgacac cactgccgat
2701 ccaaagccaa gcgctcacgc caccaccaga accctcgggc tgaagcgccc tgctatgtct
2761 cacacccctc acccgtgcca ccctccaccc cctggcccac cccaccagcc actaccccct
2821 tcccagcggt tgtccagccc taccctctcc cagtgttctc tcctcgagga ggcccccagc
2881 ctcttccccc tgctcccaca tccgtgcccc cagctgcttt ccccgcccct ttggtgaccc
2941 caatggtggc cttggtgctc cctaactatc tgttcccaac cccatccagc tatccttatg
3001 gggcactcca gacccctgct gaagggcctc ccactcctgc ctcgcactcc ccttctccat
3061 ccttgcccgc cctccccccg agtcctcctc accgcccgga ctctccactg ttcaactcga
3121 gatgcagctc tccactccag ctcaatctgc tgcagctgga ggagctcccc cgtgctgagg
3181 gggctgctgt tgcaggaggc cctgggagca gtgccgggcc cccacctccc agtgcggagg
3241 ctgctgagcc agaggccaga ctggcggagg tcactgagtc ctccaatcag gacgcacttt
3301 ccggctccag tgacctgctc gaacttctgc tgcaagagga ctcgcgctcc ggcacaggct
3361 ccgcagcctc gggctccttg ggctctggct tgggctctgg gtctggttca ggctcccatg
3421 aagggggcag cacctcagcc agcatcactc gcagcagcca gagcagccac acaagcaaat
3481 actttggcag catcgactct tccgaggctg aggctggggc tgctcggggc ggggctgagc
3541 ctggggacca ggtgattaag tacgtgctcc aggatcccat ttggctgctc atggccaatg
3601 ctgaccagcg cgtcatgatg acctaccagg tgccctccag ggacatgacc tctgtgctga
3661 agcaggatcg ggagcggctc cgagccatgc agaagcagca gcctcggttt tctgaggacc
3721 agcggcggga actgggtgct gtgcactcct gggtccggaa gggccaactg cctcgggctc
3781 ttgatgtgat ggcctgtgtg gactgtggga gcagcaccca agatcctggt caccctgatg
3841 acccactctt ctcagagctg gatggactgg ggctggagcc catggaagag ggtggaggcg
3901 agcagggcag cagcggtggc ggcagtggtg agggagaggg ctgcgaggag gcccaaggcg
3961 gggccaaggc ttcaagctct caggacttgg ctatggagga ggaggaagaa ggcaggagct
4021 catccagtcc agccttacct acagcaggaa actgcaccag ctagttgcca actttcttgt
4081 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
4141 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
4201 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
4261 ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct
4321 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
4381 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
4441 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
4501 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
4561 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
4621 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
4681 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
4741 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
4801 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
4861 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
4921 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
4981 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
5041 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
5101 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
5161 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
5221 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
5281 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
5341 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
5401 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
5461 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
5521 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
5581 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
5641 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
5701 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
5761 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
5821 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
5881 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
5941 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
6001 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
6061 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
6121 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
6181 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
6241 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
6301 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
6361 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
6421 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
6481 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
6541 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
6601 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
6661 atgcct
//
