| 出品人: | Addgene |
|---|---|
| 载体名称: | pMSCV-FLIP-puro-dsRed-GFP-miRNA |
| 质粒类型: | 逆病毒载体；RNAi载体；Cre/Lox重组系统 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 8376 bp |
| 5' 测序引物及序列: | pLXSN 5' primer 5' CCCTTGAACCTCCTCGTTCGACC3' |
| 3' 测序引物及序列: | -- |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | DH5α、Stbl3等 |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: |
推荐使用逆病毒包装载体：pUMVC、pCMV-VSV-G 。 |
| 产品目录号: | #32704 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMSCV-FLIP-puro-dsRed-GFP-miRNA 8376 bp DNA SYN
DEFINITION pMSCV-FLIP-puro-dsRed-GFP-miRNA
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8376
/organism="pMSCV-FLIP-puro-dsRed-GFP-miRNA"
/mol_type="other DNA"
misc_feature 655..1404
/label="psi_plus_pack"
misc_feature 991..1398
/label="gag"
misc_feature 1334..1356
/label="pLXSN_5_primer"
misc_feature 1334..1356
/label="MSCV_primer"
misc_feature 1372..1388
/label="pBABE_5_primer"
misc_feature complement(1417..1450)
/label="loxP"
misc_feature 1417..1450
/label="loxP"
CDS 1467..2852
/label="ORF frame 3"
/translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL
LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
ERAEARHHLFQ*"
gene 1479..2054
/label="puro (variant)"
/gene="puro (variant)"
/translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL
LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
ERAEARHHLFQ*"
misc_feature complement(2355..2375)
/label="dsRed1_N_primer"
/translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL
LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
ERAEARHHLFQ*"
misc_feature 2767..2790
/label="dsRed1_C_primer"
/translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL
LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
ERAEARHHLFQ*"
CDS complement(3152..3871)
/label="ORF frame 3"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK*"
gene complement(3155..3868)
/label="EGFP (variant)"
/gene="EGFP (variant)"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK*"
misc_feature complement(3197..3218)
/label="EGFP_C_primer"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK*"
misc_feature 3805..3826
/label="EGFP_N_primer"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK*"
misc_feature 3885..3918
/label="loxP"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK*"
misc_feature complement(3965..3998)
/label="loxP"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK*"
misc_feature 3965..3998
/label="loxP"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK*"
CDS 3978..4655
/label="ORF frame 3"
/translation="MCTIRSYPGLNDLQPSLSIIPDNQPLDYKICERLTGILNYVAPF
TLCGYAALMPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPT
GWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPW
LLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASP
HRELSIK*"
misc_feature 4034..4638
/label="WPRE"
/translation="MCTIRSYPGLNDLQPSLSIIPDNQPLDYKICERLTGILNYVAPF
TLCGYAALMPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPT
GWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPW
LLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASP
HRELSIK*"
CDS complement(4131..4634)
/label="ORF frame 2"
/translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
NSMIQRH*"
promoter complement(5369..5387)
/label="M13_reverse_primer"
/translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
NSMIQRH*"
misc_feature complement(5386..5408)
/label="M13_pUC_rev_primer"
/translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
NSMIQRH*"
promoter complement(5422..5451)
/label="lac_promoter"
/translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
NSMIQRH*"
gene complement(6534..7394)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
NSMIQRH*"
CDS complement(6534..7394)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(7436..7464)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(7623..7645)
/label="pGEX_3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(7844..7975)
/label="lacZ_a"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 7958..7980
/label="M13_pUC_fwd_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 8156..8175
/label="pBRrevBam_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT
61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA
121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA
181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT
241 CCAGGGTGCC CCAAGGACCT GAAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT
301 CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC
361 CCTCACTCGG CGCGCCAGTC CTCCGATAGA CTGCGTCGCC CGGGTACCCG TATTCCCAAT
421 AAAGCCTCTT GCTGTTTGCA TCCGAATCGT GGACTCGCTG ATCCTTGGGA GGGTCTCCTC
481 AGATTGATTG ACTGCCCACC TCGGGGGTCT TTCATTTGGA GGTTCCACCG AGATTTGGAG
541 ACCCCTGCCT AGGGACCACC GACCCCCCCG CCGGGAGGTA AGCTGGCCAG CGGTCGTTTC
601 GTGTCTGTCT CTGTCTTTGT GCGTGTTTGT GCCGGCATCT AATGTTTGCG CCTGCGTCTG
661 TACTAGTTAG CTAACTAGCT CTGTATCTGG CGGACCCGTG GTGGAACTGA CGAGTTCTGA
721 ACACCCGGCC GCAACCCTGG GAGACGTCCC AGGGACTTTG GGGGCCGTTT TTGTGGCCCG
781 ACCTGAGGAA GGGAGTCGAT GTGGAATCCG ACCCCGTCAG GATATGTGGT TCTGGTAGGA
841 GACGAGAACC TAAAACAGTT CCCGCCTCCG TCTGAATTTT TGCTTTCGGT TTGGAACCGA
901 AGCCGCGCGT CTTGTCTGCT GCAGCGCTGC AGCATCGTTC TGTGTTGTCT CTGTCTGACT
961 GTGTTTCTGT ATTTGTCTGA AAATTAGGGC CAGACTGTTA CCACTCCCTT AAGTTTGACC
1021 TTAGGTCACT GGAAAGATGT CGAGCGGATC GCTCACAACC AGTCGGTAGA TGTCAAGAAG
1081 AGACGTTGGG TTACCTTCTG CTCTGCAGAA TGGCCAACCT TTAACGTCGG ATGGCCGCGA
1141 GACGGCACCT TTAACCGAGA CCTCATCACC CAGGTTAAGA TCAAGGTCTT TTCACCTGGC
1201 CCGCATGGAC ACCCAGACCA GGTCCCCTAC ATCGTGACCT GGGAAGCCTT GGCTTTTGAC
1261 CCCCCTCCCT GGGTCAAGCC CTTTGTACAC CCTAAGCCTC CGCCTCCTCT TCCTCCATCC
1321 GCCCCGTCTC TCCCCCTTGA ACCTCCTCGT TCGACCCCGC CTCGATCCTC CCTTTATCCA
1381 GCCCTCACTC CTTCTCTAGG CGCCGGAATT AGAtccataa cttcgtatag gataccttat
1441 acgaagttat ctcaggtacc GCCACCATGG TGGAGTACAA GCCCACGGTG CGCCTCGCCA
1501 CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG
1561 CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC
1621 TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG
1681 CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG
1741 GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC
1801 TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC
1861 CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG
1921 AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG
1981 AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT
2041 GCATGACCCG CAAGCCCGGT GCCCTGTACA AGAAACAGAA AATTGTGGCA CCAGTGAAAC
2101 AGACTTTGAA TTTTGACCTT CTCAAGTTGG CGGGAGACGT CGAGTCCAAC CCTGGGCCCG
2161 GCCCGGTCGC CACCATGGAT AGCACTGAGA ACGTCATCAA GCCCTTCATG CGCTTCAAGG
2221 TGCACATGGA GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCA
2281 AGCCCTACGA GGGCACCCAG ACCGCCAAGC TGCAGGTGAC CAAGGGCGGC CCCCTGCCCT
2341 TCGCCTGGGA CATCCTGTCC CCCCAGTTCC AGTACGGCTC CAAGGTGTAC GTGAAGCACC
2401 CCGCCGACAT CCCCGACTAC AAGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG
2461 TGATGAACTT CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG
2521 GCACCTTCAT CTACCACGTG AAGTTCATCG GCGTGAACTT CCCCTCCGAC GGCCCCGTAA
2581 TGCAGAAGAA GACTCTGGGC TGGGAGCCCT CCACCGAGCG CCTGTACCCC CGCGACGGCG
2641 TGCTGAAGGG CGAGATCCAC AAGGCGCTGA AGCTGAAGGG CGGCGGCCAC TACCTGGTGG
2701 AGTTCAAGTC AATCTACATG GCCAAGAAGC CCGTGAAGCT GCCCGGCTAC TACTACGTGG
2761 ACTCCAAGCT GGACATCACC TCCCACAACG AGGACTACAC CGTGGTGGAG CAGTACGAGC
2821 GCGCCGAGGC CCGCCACCAC CTGTTCCAGT AGtctagaag ccataacttc gtatagtaca
2881 cattatacga aGTTATGCAT ACCTGGAGGA AAAAAAAAAG GGGAGAGTCA GGGGTTTAAA
2941 CTCGAGTGCG GCCAGATCTG GGCCATTTGT TCCATGTGAG TGCTAGTAAC AGGCCTTGTG
3001 TCCTGACAAC AGGCAGCATA ATGAGTCAGT CAGTGGCCAA AACTCATTAT GGACTGCCTG
3061 TTGTCAGCAT ACAGCCTTCA GCAAGCCTCC AGGATCCACT GGTCGACTCA CTACCTCCCT
3121 TTAAACGATC GACGGCCACG AAGTGCTTAG CTTACTTGTA CAGCTCGTCC ATGCCGAGAG
3181 TGATCCCGGC GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT
3241 CTTTGCTCAG GGCGGACTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT
3301 CGCCGATGGG GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT
3361 TGTGGCGGGT CTTGAAGTTC ACCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA
3421 CCTTGTGGCT GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA
3481 AGTCGATGCC CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG
3541 CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT
3601 CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC
3661 ACTGCACGCC GTAGGTGAAG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG
3721 TGGTGCAGAT GAACTTCAGG GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG
3781 ACACGCTGAA CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC
3841 CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGGTGGCGac cggtataact tcgtataagg
3901 tatcctatac gaagttatcc attcaggctg tgctagcatc aatggcatgg cacaaagctt
3961 agccataact tcgtataatg tgtactatac gaagttatcc cgggttAAAC GACCTGCAGC
4021 CAAGCTTATC GATaattccc gataatcaac ctctggatta caaaatttgt gaaagattga
4081 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
4141 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
4201 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
4261 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
4321 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
4381 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
4441 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
4501 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
4561 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
4621 ccgcctcccc gcatcgggaa ttATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA
4681 AGGGGGGAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
4741 GCAAGGCATG GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA
4801 GAGACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
4861 GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT AGAGAACCAT
4921 CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAAATGACCC TGTGCCTTAT TTGAACTAAC
4981 CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG
5041 CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT
5101 GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG TCTCGCTGTT CCTTGGGAGG
5161 GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT TCATGGGTAA CAGTTTCTTG
5221 AAGTTGGAGA ACAACATTCT GAGGGTAGGA GTCGAATATT AAGTAATCCT GACTCAATTA
5281 GCCACTGTTT TGAATCCACA TACTCCAATA CTCCTGAAAT AGTTCATTAT GGACAGCGCA
5341 GAAGAGCTGG GGAGAATTAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT
5401 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT
5461 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG
5521 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG
5581 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
5641 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
5701 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
5761 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
5821 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
5881 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
5941 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG
6001 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
6061 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
6121 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
6181 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG
6241 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC
6301 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
6361 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
6421 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
6481 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
6541 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
6601 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
6661 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
6721 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
6781 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
6841 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
6901 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
6961 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
7021 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
7081 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
7141 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
7201 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
7261 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
7321 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
7381 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
7441 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
7501 ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC
7561 TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA
7621 AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG
7681 AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC
7741 TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC
7801 AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT GCGCAACTGT
7861 TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT
7921 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
7981 ACGGCGCAAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA
8041 ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC
8101 CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA
8161 TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC
8221 GTCCGGCGTA GAGGCGATTA GTCCAATTTG TTAAAGACAG GATATCAGTG GTCCAGGCTC
8281 TAGTTTTGAC TCAACAATAT CACCAGCTGA AGCCTATAGA GTACGAGCCA TAGATAAAAT
8341 AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAA
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