pMSCV-FLIP-puro-dsRed-GFP-miRNA

价格:1350元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品人: Addgene
载体名称: pMSCV-FLIP-puro-dsRed-GFP-miRNA
质粒类型: 逆病毒载体;RNAi载体;Cre/Lox重组系统
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 8376 bp
5' 测序引物及序列: pLXSN 5' primer 5' CCCTTGAACCTCCTCGTTCGACC3'
3' 测序引物及序列: --
载体标签:
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: DH5α、Stbl3等
宿主细胞(系): 常规哺乳动物细胞系
备注:

推荐使用逆病毒包装载体:pUMVCpCMV-VSV-G
 
产品目录号: #32704
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pMSCV-FLIP-puro-dsRed-GFP-miRNA载体图谱



载体简介

载体序列

LOCUS       pMSCV-FLIP-puro-dsRed-GFP-miRNA	8376 bp 	DNA	SYN
DEFINITION  pMSCV-FLIP-puro-dsRed-GFP-miRNA
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="pMSCV-FLIP-puro-dsRed-GFP-miRNA"
                     /mol_type="other DNA"
     misc_feature    655..1404
                     /label="psi_plus_pack"
     misc_feature    991..1398
                     /label="gag"
     misc_feature    1334..1356
                     /label="pLXSN_5_primer"
     misc_feature    1334..1356
                     /label="MSCV_primer"
     misc_feature    1372..1388
                     /label="pBABE_5_primer"
     misc_feature    complement(1417..1450)
                     /label="loxP"
     misc_feature    1417..1450
                     /label="loxP"
     CDS             1467..2852
                     /label="ORF frame 3"
                     /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL
                     LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     gene            1479..2054
                     /label="puro (variant)"
                     /gene="puro (variant)"
                     /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL
                     LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     misc_feature    complement(2355..2375)
                     /label="dsRed1_N_primer"
                     /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL
                     LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     misc_feature    2767..2790
                     /label="dsRed1_C_primer"
                     /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL
                     LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     CDS             complement(3152..3871)
                     /label="ORF frame 3"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     gene            complement(3155..3868)
                     /label="EGFP (variant)"
                     /gene="EGFP (variant)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(3197..3218)
                     /label="EGFP_C_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    3805..3826
                     /label="EGFP_N_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    3885..3918
                     /label="loxP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(3965..3998)
                     /label="loxP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    3965..3998
                     /label="loxP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     CDS             3978..4655
                     /label="ORF frame 3"
                     /translation="MCTIRSYPGLNDLQPSLSIIPDNQPLDYKICERLTGILNYVAPF
                     TLCGYAALMPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPT
                     GWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPW
                     LLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASP
                     HRELSIK*"
     misc_feature    4034..4638
                     /label="WPRE"
                     /translation="MCTIRSYPGLNDLQPSLSIIPDNQPLDYKICERLTGILNYVAPF
                     TLCGYAALMPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPT
                     GWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPW
                     LLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASP
                     HRELSIK*"
     CDS             complement(4131..4634)
                     /label="ORF frame 2"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     promoter        complement(5369..5387)
                     /label="M13_reverse_primer"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    complement(5386..5408)
                     /label="M13_pUC_rev_primer"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     promoter        complement(5422..5451)
                     /label="lac_promoter"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     gene            complement(6534..7394)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     CDS             complement(6534..7394)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(7436..7464)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(7623..7645)
                     /label="pGEX_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(7844..7975)
                     /label="lacZ_a"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    7958..7980
                     /label="M13_pUC_fwd_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    8156..8175
                     /label="pBRrevBam_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT
   61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA
  121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA
  181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT
  241 CCAGGGTGCC CCAAGGACCT GAAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT
  301 CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC
  361 CCTCACTCGG CGCGCCAGTC CTCCGATAGA CTGCGTCGCC CGGGTACCCG TATTCCCAAT
  421 AAAGCCTCTT GCTGTTTGCA TCCGAATCGT GGACTCGCTG ATCCTTGGGA GGGTCTCCTC
  481 AGATTGATTG ACTGCCCACC TCGGGGGTCT TTCATTTGGA GGTTCCACCG AGATTTGGAG
  541 ACCCCTGCCT AGGGACCACC GACCCCCCCG CCGGGAGGTA AGCTGGCCAG CGGTCGTTTC
  601 GTGTCTGTCT CTGTCTTTGT GCGTGTTTGT GCCGGCATCT AATGTTTGCG CCTGCGTCTG
  661 TACTAGTTAG CTAACTAGCT CTGTATCTGG CGGACCCGTG GTGGAACTGA CGAGTTCTGA
  721 ACACCCGGCC GCAACCCTGG GAGACGTCCC AGGGACTTTG GGGGCCGTTT TTGTGGCCCG
  781 ACCTGAGGAA GGGAGTCGAT GTGGAATCCG ACCCCGTCAG GATATGTGGT TCTGGTAGGA
  841 GACGAGAACC TAAAACAGTT CCCGCCTCCG TCTGAATTTT TGCTTTCGGT TTGGAACCGA
  901 AGCCGCGCGT CTTGTCTGCT GCAGCGCTGC AGCATCGTTC TGTGTTGTCT CTGTCTGACT
  961 GTGTTTCTGT ATTTGTCTGA AAATTAGGGC CAGACTGTTA CCACTCCCTT AAGTTTGACC
 1021 TTAGGTCACT GGAAAGATGT CGAGCGGATC GCTCACAACC AGTCGGTAGA TGTCAAGAAG
 1081 AGACGTTGGG TTACCTTCTG CTCTGCAGAA TGGCCAACCT TTAACGTCGG ATGGCCGCGA
 1141 GACGGCACCT TTAACCGAGA CCTCATCACC CAGGTTAAGA TCAAGGTCTT TTCACCTGGC
 1201 CCGCATGGAC ACCCAGACCA GGTCCCCTAC ATCGTGACCT GGGAAGCCTT GGCTTTTGAC
 1261 CCCCCTCCCT GGGTCAAGCC CTTTGTACAC CCTAAGCCTC CGCCTCCTCT TCCTCCATCC
 1321 GCCCCGTCTC TCCCCCTTGA ACCTCCTCGT TCGACCCCGC CTCGATCCTC CCTTTATCCA
 1381 GCCCTCACTC CTTCTCTAGG CGCCGGAATT AGAtccataa cttcgtatag gataccttat
 1441 acgaagttat ctcaggtacc GCCACCATGG TGGAGTACAA GCCCACGGTG CGCCTCGCCA
 1501 CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG
 1561 CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC
 1621 TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG
 1681 CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG
 1741 GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC
 1801 TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC
 1861 CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG
 1921 AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG
 1981 AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT
 2041 GCATGACCCG CAAGCCCGGT GCCCTGTACA AGAAACAGAA AATTGTGGCA CCAGTGAAAC
 2101 AGACTTTGAA TTTTGACCTT CTCAAGTTGG CGGGAGACGT CGAGTCCAAC CCTGGGCCCG
 2161 GCCCGGTCGC CACCATGGAT AGCACTGAGA ACGTCATCAA GCCCTTCATG CGCTTCAAGG
 2221 TGCACATGGA GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCA
 2281 AGCCCTACGA GGGCACCCAG ACCGCCAAGC TGCAGGTGAC CAAGGGCGGC CCCCTGCCCT
 2341 TCGCCTGGGA CATCCTGTCC CCCCAGTTCC AGTACGGCTC CAAGGTGTAC GTGAAGCACC
 2401 CCGCCGACAT CCCCGACTAC AAGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG
 2461 TGATGAACTT CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG
 2521 GCACCTTCAT CTACCACGTG AAGTTCATCG GCGTGAACTT CCCCTCCGAC GGCCCCGTAA
 2581 TGCAGAAGAA GACTCTGGGC TGGGAGCCCT CCACCGAGCG CCTGTACCCC CGCGACGGCG
 2641 TGCTGAAGGG CGAGATCCAC AAGGCGCTGA AGCTGAAGGG CGGCGGCCAC TACCTGGTGG
 2701 AGTTCAAGTC AATCTACATG GCCAAGAAGC CCGTGAAGCT GCCCGGCTAC TACTACGTGG
 2761 ACTCCAAGCT GGACATCACC TCCCACAACG AGGACTACAC CGTGGTGGAG CAGTACGAGC
 2821 GCGCCGAGGC CCGCCACCAC CTGTTCCAGT AGtctagaag ccataacttc gtatagtaca
 2881 cattatacga aGTTATGCAT ACCTGGAGGA AAAAAAAAAG GGGAGAGTCA GGGGTTTAAA
 2941 CTCGAGTGCG GCCAGATCTG GGCCATTTGT TCCATGTGAG TGCTAGTAAC AGGCCTTGTG
 3001 TCCTGACAAC AGGCAGCATA ATGAGTCAGT CAGTGGCCAA AACTCATTAT GGACTGCCTG
 3061 TTGTCAGCAT ACAGCCTTCA GCAAGCCTCC AGGATCCACT GGTCGACTCA CTACCTCCCT
 3121 TTAAACGATC GACGGCCACG AAGTGCTTAG CTTACTTGTA CAGCTCGTCC ATGCCGAGAG
 3181 TGATCCCGGC GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT
 3241 CTTTGCTCAG GGCGGACTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT
 3301 CGCCGATGGG GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT
 3361 TGTGGCGGGT CTTGAAGTTC ACCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA
 3421 CCTTGTGGCT GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA
 3481 AGTCGATGCC CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG
 3541 CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT
 3601 CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC
 3661 ACTGCACGCC GTAGGTGAAG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG
 3721 TGGTGCAGAT GAACTTCAGG GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG
 3781 ACACGCTGAA CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC
 3841 CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGGTGGCGac cggtataact tcgtataagg
 3901 tatcctatac gaagttatcc attcaggctg tgctagcatc aatggcatgg cacaaagctt
 3961 agccataact tcgtataatg tgtactatac gaagttatcc cgggttAAAC GACCTGCAGC
 4021 CAAGCTTATC GATaattccc gataatcaac ctctggatta caaaatttgt gaaagattga
 4081 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
 4141 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
 4201 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
 4261 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
 4321 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
 4381 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
 4441 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
 4501 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
 4561 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
 4621 ccgcctcccc gcatcgggaa ttATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA
 4681 AGGGGGGAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
 4741 GCAAGGCATG GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA
 4801 GAGACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
 4861 GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT AGAGAACCAT
 4921 CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAAATGACCC TGTGCCTTAT TTGAACTAAC
 4981 CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG
 5041 CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT
 5101 GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG TCTCGCTGTT CCTTGGGAGG
 5161 GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT TCATGGGTAA CAGTTTCTTG
 5221 AAGTTGGAGA ACAACATTCT GAGGGTAGGA GTCGAATATT AAGTAATCCT GACTCAATTA
 5281 GCCACTGTTT TGAATCCACA TACTCCAATA CTCCTGAAAT AGTTCATTAT GGACAGCGCA
 5341 GAAGAGCTGG GGAGAATTAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT
 5401 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT
 5461 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG
 5521 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG
 5581 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
 5641 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
 5701 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
 5761 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
 5821 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
 5881 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
 5941 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG
 6001 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
 6061 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
 6121 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
 6181 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG
 6241 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC
 6301 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
 6361 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
 6421 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
 6481 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
 6541 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
 6601 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
 6661 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
 6721 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
 6781 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
 6841 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
 6901 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
 6961 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
 7021 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
 7081 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
 7141 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
 7201 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
 7261 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
 7321 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
 7381 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
 7441 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
 7501 ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC
 7561 TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA
 7621 AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG
 7681 AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC
 7741 TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC
 7801 AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT GCGCAACTGT
 7861 TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT
 7921 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
 7981 ACGGCGCAAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA
 8041 ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC
 8101 CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA
 8161 TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC
 8221 GTCCGGCGTA GAGGCGATTA GTCCAATTTG TTAAAGACAG GATATCAGTG GTCCAGGCTC
 8281 TAGTTTTGAC TCAACAATAT CACCAGCTGA AGCCTATAGA GTACGAGCCA TAGATAAAAT
 8341 AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAA
//

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