pFB

价格:3000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Agilent
载体名称: pFB
质粒类型: 逆病毒载体
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: --
载体大小: 5117 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记:
克隆菌株: DH5α 等通用菌株
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: --
产品目录号: #217563
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pFB载体图谱



pFB 多克隆位点

pFB 载体特征

载体简介

pFB载体描述

The Agilent pFB and pFB-Neo plasmid vectors for retroviral gene delivery and expression are derived from the Moloney murine leukemia virus (MMLV) and can be used to produce high titer viral stocks with MMLVbased packaging cell lines.1,2 Both vectors contain the bacterial origin of replication and ampicillin-resistance gene from pBR322, an extended MMLV packaging signal (ψ+), and a multiple cloning site (MCS) located between the MMLV 5′ and 3′ long terminal repeat sequences (LTRs).
The pFB vector is a minimal MMLV-based vector that can accommodate larger inserts than pFB-Neo. The pFB vector does not contain any exogenous sequence other than the sequence of the MCS.
The pFB-Neo vector differs from the pFB vector only by the presence of a cassette consisting of the internal ribosome entry site (IRES) from the encephalomyocarditis virus (EMCV) and the neomycin-resistance gene (neor). The open reading frame (ORF) for the neor gene is positioned downstream of the MCS and follows the IRES. A dicistronic message encoding the gene of interest and the neor gene is expressed from the viral promoter within the 5′ LTR.



pFB载体限制性酶切位点

pFB, 5117 bp			             version 013001


Enzymes with 1-10 cleavage sites:
           
            #sites   --  Bp position of recognition site --

  AatII          2      978,   5043
  Acc65I         3      647,   1846,   2643
  AccI           3     1967,   2057,   3003
  AflII          3      202,   1265,   2198
  AflIII         2      164,   3232
  AgeI           1     1840
  AhdI           6      687,    733,   1274,   2683,   2729
                       4120
  Alw44I         4     1054,   3048,   3546,   4792
  AlwNI          5      323,    398,   2319,   2394,   3643
  ApaI           1     1238
  ApoI           3       87,   1128,   2063
  AvaI           9      577,    610,    643,   1241,   2018
                       2074,   2573,   2606,   2639
  BamHI          1     2069
  BanII          7      580,    593,    764,   1238,   2576
                       2589,   2760
  BbeI           3      615,   1656,   2611
  BbsI           1     5110
  BbvCI          2      453,   2449
  BciVI          5      656,   1986,   2652,   3441,   4968
  BglI           1     4239
  BglII          1     1678
  BpmI           3     1799,   2146,   4210
  Bpu10I         7      337,    412,    453,   1548,   2333
                       2408,   2449
  BsaAI          3     1816,   2000,   2984
  BsaHI          6      615,    978,   1656,   2611,   4661
                       5043
  BsaI           9      694,    715,    782,   1414,   1802
                       2690,   2711,   2778,   4192
  BsaWI          5      138,   1840,   3438,   3585,   4416
  BseRI          5      718,   1068,   1561,   1600,   2714
  BsiEI          8      961,   1034,   1838,   2080,   3145
                       3569,   4492,   4641
  BsiHKAI        7      580,   1054,   2576,   3048,   3546
                       4707,   4792
  BsmBI          6      976,   1093,   1337,   1396,   1582
                       2881
  Bsp120I        1     1238
  BspHI          3     3952,   4960,   5065
  BspLU11I       2      164,   3232
  BsrBI          5     1299,   2077,   2102,   3163,   4964
  BsrDI          2     4179,   4361
  BsrGI          1     1541
  BssHII         2      563,   2559
  BssSI          3     3405,   4789,   5096
  Bst1107I       1     3003
  BstAPI         5      322,    397,   2318,   2393,   3050
  BstEII         1     1346
  BstF5I         9      682,   1386,   1573,   2031,   2678
                       2907,   4105,   4286,   4573
  Bsu36I         1     1276
  Cfr10I         2     1840,   4205
  Csp6I          8      648,    895,   1542,   1819,   1847
                       2644,   3039,   4604
  DraI           3     3989,   4008,   4700
  DraIII         1     1873
  DrdI           2     2921,   3334
  DsaI           2      151,    931
  EaeI           7      827,    961,   1368,   1389,   1668
                       2080,   4513
  EarI           4     1334,   1564,   3110,   4914
  Ecl136II       2      580,   2576
  Eco52I         2      961,   2080
  Eco57I         2     3759,   4807
  Eco72I         1     2000
  EcoNI          1     1647
  EcoO109I       5      499,   1477,   1924,   2495,   5100
  EcoRI          1     2063
  EcoRV          4      308,    383,   2304,   2379
  EheI           3      615,   1656,   2611
  FokI           9      682,   1386,   1573,   2031,   2678
                       2907,   4105,   4286,   4573
  FspI           1     4345
  HaeII          5      615,   1656,   2611,   3106,   3476
  HgaI           6      889,   1166,   2938,   3334,   3912
                       4662
  HincII         2     2057,   4664
  HinfI         10      637,   1020,   1045,   1863,   2108
                       2633,   3132,   3207,   3603,   4120
  KasI           3      615,   1656,   2611
  KpnI           3      647,   1846,   2643
  MaeII         10      979,   1340,   1381,   1817,   2001
                       2985,   3935,   4351,   4724,   5044
  MboII         10     1334,   1565,   2096,   3111,   3882
                       3973,   4728,   4806,   4915,   5111
  MscI           3      827,   1368,   1668
  MslI           4      159,   4373,   4532,   4891
  MunI           2       11,     21
  NarI           3      615,   1656,   2611
  NdeI           3     1664,   1672,   3054
  NheI           5        6,     16,     26,    197,   2193
  NotI           1     2079
  NspI           3      164,   2867,   3232
  PleI           9      637,   1020,   1045,   1863,   2108
                       2633,   3132,   3603,   4120
  PpuMI          4      499,   1477,   1924,   2495
  PshAI          1     1015
  Psp1406I       2     4350,   4723
  PstI           3     1178,   1360,   4366
  PvuI           2     1034,   4492
  PvuII          4      286,    361,   2282,   2357
  RsaI           8      648,    895,   1542,   1819,   1847
                       2644,   3039,   4604
  SacI           2      580,   2576
  SacII          1      151
  SalI           1     2057
  SapI           1     3109
  ScaI           1     4603
  SchI           9      637,   1020,   1045,   1863,   2108
                       2633,   3132,   3603,   4120
  SexAI          1     1474
  SfcI           7      182,   1178,   1360,   2178,   3497
                       3688,   4366
  SmaI           4      643,   1241,   2018,   2639
  SmlI           8      202,   1265,   2074,   2198,   3338
                       3600,   3877,   4745
  SpeI           1      897
  SrfI           1     1240
  SspI           1     4927
  StyI           5      496,    706,   1504,   2492,   2702
  TaiI          10      979,   1340,   1381,   1817,   2001
                       2985,   3935,   4351,   4724,   5044
  TaqI           9     1297,   1608,   1619,   2050,   2058
                       2075,   2119,   3332,   4776
  TatI           3     1541,   3038,   4603
  TfiI           1     3207
  Tsp45I         6     1282,   1491,   2887,   2982,   4382
                       4593
  TspRI         10     1284,   1875,   3128,   3634,   3647
                       3918,   4067,   4172,   4519,   4546
  Tth111I        4      633,   1473,   2629,   2976
  VspI           1     4296
  XbaI           3      464,   2036,   2460
  XhoI           1     2074
  XhoII          8     1678,   2069,   3873,   3884,   3970
                       3982,   4750,   4767
  XmaI           4      643,   1241,   2018,   2639
  XmnI           1     4720





Enzymes that do NOT cut molecule:

AccIII      AscI        BclI        BlnI        Bpu1102I    
BsaBI       BsgI        BsiWI       BsmI        BspMI       
BstBI       BstXI       ClaI        Eco47III    FseI        
HindIII     HpaI        MluI        NaeI        NcoI        
NgoMIV      NruI        NsiI        PacI        PmeI        
Ppu10I      RsrII       SanDI       SbfI        SfiI        
SgfI        SgrAI       SnaBI       SphI        StuI        
SwaI        Van91I      XcmI        


载体序列

pFB, 5117 bp                                  version 013001

NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been 
obtained from existing data. 

   1  GAATTGCTAG CAATTGCTAG CAATTGCTAG CAATTCATAC CAGATCACCG  
  51  AAAACTGTCC TCCAAATGTG TCCCCCTCAC ACTCCCAAAT TCGCGGGCTT  
 101  CTGCCTCTTA GACCACTCTA CCCTATTCCC CACACTCACC GGAGCCAAAG  
 151  CCGCGGGACA TATACATGTG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA  
 201  GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT  
 251  AGAAAAGTTC AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC  
 301  CAAAGCGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA  
 351  ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG  
 401  TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA  
 451  GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG  
 501  GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC  
 551  TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC  
 601  AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA  
 651  CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG  
 701  CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG  
 751  GTCTTTCATT TGGGGGCTCG TCCGGGATCG GGAGACCCCT GCCCAGGGAC  
 801  CACCGACCCA CCACCGGGAG GTAAGCTGGC CAGCAACTTA TCTGTGTCTG  
 851  TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA  
 901  GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT  
 951  TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTCGGGGGC  
1001  CGTTTTTGTG GCCCGACCTG AGTCCAAAAA TCCCGATCGT TTTGGACTCT  
1051  TTGGTGCACC CCCCTTAGAG GAGGGATATG TGGTTCTGGT AGGAGACGAG  
1101  AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGGA  
1151  CCGAAGCCGC GCCGCGCGTC TTGTCTGCTG CAGCATCGTT CTGTGTTGTC  
1201  TCTGTCTGAC TGTGTTTCTG TATTTGTCTG AAAATATGGG CCCGGGCCAG  
1251  ACTGTTACCA CTCCCTTAAG TTTGACCTTA GGTCACTGGA AAGATGTCGA  
1301  GCGGATCGCT CACAACCAGT CGGTAGATGT CAAGAAGAGA CGTTGGGTTA  
1351  CCTTCTGCTC TGCAGAATGG CCAACCTTTA ACGTCGGATG GCCGCGAGAC  
1401  GGCACCTTTA ACCGAGACCT CATCACCCAG GTTAAGATCA AGGTCTTTTC  
1451  ACCTGGCCCG CATGGACACC CAGACCAGGT CCCCTACATC GTGACCTGGG  
1501  AAGCCTTGGC TTTTGACCCC CCTCCCTGGG TCAAGCCCTT TGTACACCCT  
1551  AAGCCTCCGC CTCCTCTTCC TCCATCCGCC CCGTCTCTCC CCCTTGAACC  
1601  TCCTCGTTCG ACCCCGCCTC GATCCTCCCT TTATCCAGCC CTCACTCCTT  
1651  CTCTAGGCGC CCCCATATGG CCATATGAGA TCTTATATGG GGCACCCCCG  
1701  CCCCTTGTAA ACTTCCCTGA CCCTGACATG ACAAGAGTTA CTAACAGCCC  
1751  CTCTCTCCAA GCTCACTTAC AGGCTCTCTA CTTAGTCCAG CACGAAGTCT  
1801  GGAGACCTCT GGCGGCACGT ACCAAGAACA ACTGGACCGA CCGGTGGTAC  
1851  CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT  
1901  AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC  
1951  CCCCACCGCC CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC  
2001  ACGTGAAGGC TGCCGACCCC GGGGGTGGAC CATCCTCTAG ACTGCCGGAT  
2051  CGAATTGTCG ACGAATTCGG ATCCTCGAGC GGCCGCGATC CGAGTTCTTC  
2101  TGAGCGGGAC TCTGGGGTTC GATAAAATAA AAGATTTTAT TTAGTCTCCA  
2151  GAAAAAGGGG GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT  
2201  AAGTAACGCC ATTTTGCAAG GCATGGAAAA ATACATAACT GAGAATAGAG  
2251  AAGTTCAGAT CAAGGTCAGG AACAGATGGA ACAGCTGAAT ATGGGCCAAA  
2301  CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG  
2351  ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC  
2401  TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC  
2451  TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC  
2501  TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC  
2551  TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC  
2601  CCTCACTCGG GGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG  
2651  TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT  
2701  TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT  
2751  TTCATTTGGG GGCTCGTCCG GGATCGGGAG ACCCCTGCCC AGGGACCACC  
2801  GACCCACCAC CGGGAGGTAA GCTGGCTGCC TCGCGCGTTT CGGTGATGAC  
2851  GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT  
2901  GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG  
2951  TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA  
3001  GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG  
3051  CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG  
3101  CATCAGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG  
3151  TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA  
3201  TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA  
3251  GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA  
3301  GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG  
3351  TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG  
3401  CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT  
3451  CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT  
3501  AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA  
3551  CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC  
3601  TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT  
3651  GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT  
3701  GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT  
3751  GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA  
3801  TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA  
3851  GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT  
3901  CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG  
3951  GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA  
4001  ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA  
4051  GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT  
4101  CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG  
4151  GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC  
4201  GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC  
4251  GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA  
4301  TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA  
4351  ACGTTGTTGC CATTGCTGCA GGCATCGTGG TGTCACGCTC GTCGTTTGGT  
4401  ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC  
4451  CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG  
4501  TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG  
4551  CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG  
4601  TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT  
4651  GCTCTTGCCC GGCGTCAACA CGGGATAATA CCGCGCCACA TAGCAGAACT  
4701  TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG  
4751  GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA  
4801  ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA  
4851  ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG  
4901  TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG  
4951  GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA  
5001  CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA  
5051  AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA  
5101  GGCCCTTTCG TCTTCAA

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:
Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng