pCFB

价格:3000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Agilent
载体名称: pCFB
质粒类型: 逆病毒载体
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 5292 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记:
克隆菌株: DH5α 等通用菌株
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pCFB载体图谱



pCFB 多克隆位点

pCFB 载体特征

载体简介

pCFB载体限制性酶切位点

pCFB, 5292 bp			               version 013001


Enzymes with 1-10 cleavage sites:
           
              #sites   --  Bp position of recognition site --

  AatII          6      328,    381,    464,    650,   1153
                       5218
  Acc65I         3      822,   2021,   2818
  AccI           3     2142,   2232,   3178
  AflII          3      202,   1440,   2373
  AflIII         2      164,   3407
  AgeI           1     2015
  AhdI           6      862,    908,   1449,   2858,   2904
                       4295
  Alw44I         4     1229,   3223,   3721,   4967
  AlwNI          3     2494,   2569,   3818
  ApaI           1     1413
  ApoI           3       87,   1303,   2238
  AvaI           8      785,    818,   1416,   2193,   2249
                       2748,   2781,   2814
  BamHI          1     2244
  BanII          5      939,   1413,   2751,   2764,   2935
  BbeI           3      790,   1831,   2786
  BbsI           1     5285
  BbvCI          1     2624
  BciVI          5      831,   2161,   2827,   3616,   5143
  BglI           4      291,    413,    484,   4414
  BglII          1     1853
  BpmI           3     1974,   2321,   4385
  Bpu10I         4     1723,   2508,   2583,   2624
  BsaAI          4      545,   1991,   2175,   3159
  BsaHI         10      328,    381,    464,    650,    790
                       1153,   1831,   2786,   4836,   5218
  BsaI           9      869,    890,    957,   1589,   1977
                       2865,   2886,   2953,   4367
  BsaWI          5      138,   2015,   3613,   3760,   4591
  BseRI          5      893,   1243,   1736,   1775,   2889
  BsiEI          8     1136,   1209,   2013,   2255,   3320
                       3744,   4667,   4816
  BsiHKAI        6     1229,   2751,   3223,   3721,   4882
                       4967
  BsmBI          6     1151,   1268,   1512,   1571,   1757
                       3056
  Bsp120I        1     1413
  BspHI          3     4127,   5135,   5240
  BspLU11I       2      164,   3407
  BsrBI          5     1474,   2252,   2277,   3338,   5139
  BsrDI          2     4354,   4536
  BsrGI          1     1716
  BssHII         1     2734
  BssSI          3     3580,   4964,   5271
  Bst1107I       1     3178
  BstAPI         3     2493,   2568,   3225
  BstEII         1     1521
  BstF5I        10      777,    857,   1561,   1748,   2206
                       2853,   3082,   4280,   4461,   4748
  Bsu36I         1     1451
  Cfr10I         2     2015,   4380
  DraI           3     4164,   4183,   4875
  DraIII         1     2048
  DrdI           2     3096,   3509
  DsaI           3      151,    567,   1106
  EaeI           7     1002,   1136,   1543,   1564,   1843
                       2255,   4688
  EarI           4     1509,   1739,   3285,   5089
  Ecl136II       1     2751
  Eco52I         2     1136,   2255
  Eco57I         2     3934,   4982
  Eco72I         1     2175
  EcoNI          1     1822
  EcoO109I       4     1652,   2099,   2670,   5275
  EcoRI          1     2238
  EcoRV          2     2479,   2554
  EheI           3      790,   1831,   2786
  FokI          10      777,    857,   1561,   1748,   2206
                       2853,   3082,   4280,   4461,   4748
  FspI           1     4520
  HaeII          5      790,   1831,   2786,   3281,   3651
  HgaI           7      724,   1064,   1341,   3113,   3509
                       4087,   4837
  HincII         2     2232,   4839
  KasI           3      790,   1831,   2786
  KpnI           3      822,   2021,   2818
  MboII         10     1509,   1740,   2271,   3286,   4057
                       4148,   4903,   4981,   5090,   5286
  MscI           3     1002,   1543,   1843
  MslI           5      159,    568,   4548,   4707,   5066
  MspA1I         9      151,    918,   2457,   2532,   2914
                       3117,   3747,   3992,   4933
  MunI           2       11,     21
  NarI           3      790,   1831,   2786
  NcoI           1      567
  NdeI           4      440,   1839,   1847,   3229
  NheI           5        6,     16,     26,    197,   2368
  NotI           1     2254
  NsiI           1      774
  NspI           4      164,    244,   3042,   3407
  PleI          10      617,    812,   1195,   1220,   2038
                       2283,   2808,   3307,   3778,   4295
  Ppu10I         1      774
  PpuMI          3     1652,   2099,   2670
  PshAI          1     1190
  Psp1406I       2     4525,   4898
  PstI           3     1353,   1535,   4541
  PvuI           2     1209,   4667
  PvuII          2     2457,   2532
  SacI           1     2751
  SacII          1      151
  SalI           1     2232
  SapI           1     3284
  ScaI           1     4778
  SchI          10      617,    812,   1195,   1220,   2038
                       2283,   2808,   3307,   3778,   4295
  SexAI          1     1649
  SfcI           7      182,   1353,   1535,   2353,   3672
                       3863,   4541
  SmaI           4      818,   1416,   2193,   2814
  SmlI           8      202,   1440,   2249,   2373,   3513
                       3775,   4052,   4920
  SnaBI          1      545
  SpeI           1     1072
  SphI           1      244
  SrfI           1     1415
  SspI           1     5102
  StyI           5      567,    881,   1679,   2667,   2877
  TaqI           9     1472,   1783,   1794,   2225,   2233
                       2250,   2294,   3507,   4951
  TatI           7      424,    504,    537,    588,   1716
                       3213,   4778
  TfiI           1     3382
  Tsp45I         6     1457,   1666,   3062,   3157,   4557
                       4768
  TspRI         10     1459,   2050,   3303,   3809,   3822
                       4093,   4242,   4347,   4694,   4721
  Tth111I        4      808,   1648,   2804,   3151
  VspI           1     4471
  XbaI           2     2211,   2635
  XhoI           1     2249
  XhoII          8     1853,   2244,   4048,   4059,   4145
                       4157,   4925,   4942
  XmaI           4      818,   1416,   2193,   2814
  XmnI           1     4895





Enzymes that do NOT cut molecule:

AccIII      AscI        BclI        BlnI        
Bpu1102I    BsaBI       BsgI        BsiWI       
BsmI        BspMI       BstBI       BstXI       
ClaI        Eco47III    FseI        HindIII     
HpaI        MluI        NaeI        NgoMIV      
NruI        PacI        PmeI        RsrII       
SanDI       SbfI        SfiI        SgfI        
SgrAI       StuI        SwaI        Van91I      
XcmI        

载体序列

pCFB, 5292 bp                                 version 013001

NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been 
obtained from existing data. 

   1  GAATTGCTAG CAATTGCTAG CAATTGCTAG CAATTCATAC CAGATCACCG  
  51  AAAACTGTCC TCCAAATGTG TCCCCCTCAC ACTCCCAAAT TCGCGGGCTT  
 101  CTGCCTCTTA GACCACTCTA CCCTATTCCC CACACTCACC GGAGCCAAAG  
 151  CCGCGGGACA TATACATGTG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA  
 201  GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACGCATGCC  
 251  CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG  
 301  CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC  
 351  CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT  
 401  TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG  
 451  TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG  
 501  CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA  
 551  TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG  
 601  GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG  
 651  ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA  
 701  TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG  
 751  GTGGGAGGTC TATATAAGCA GAGATGCATC CTCACTCGGG GCGCCAGTCC  
 801  TCCGATTGAC TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG  
 851  CAGTTGCATC CGACTTGTGG TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG  
 901  AGTGATTGAC TACCCGTCAG CGGGGGTCTT TCATTTGGGG GCTCGTCCGG  
 951  GATCGGGAGA CCCCTGCCCA GGGACCACCG ACCCACCACC GGGAGGTAAG  
1001  CTGGCCAGCA ACTTATCTGT GTCTGTCCGA TTGTCTAGTG TCTATGACTG  
1051  ATTTTATGCG CCTGCGTCGG TACTAGTTAG CTAACTAGCT CTGTATCTGG  
1101  CGGACCCGTG GTGGAACTGA CGAGTTCGGA ACACCCGGCC GCAACCCTGG  
1151  GAGACGTCCC AGGGACTTCG GGGGCCGTTT TTGTGGCCCG ACCTGAGTCC  
1201  AAAAATCCCG ATCGTTTTGG ACTCTTTGGT GCACCCCCCT TAGAGGAGGG  
1251  ATATGTGGTT CTGGTAGGAG ACGAGAACCT AAAACAGTTC CCGCCTCCGT  
1301  CTGAATTTTT GCTTTCGGTT TGGGACCGAA GCCGCGCCGC GCGTCTTGTC  
1351  TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT CTGACTGTGT TTCTGTATTT  
1401  GTCTGAAAAT ATGGGCCCGG GCCAGACTGT TACCACTCCC TTAAGTTTGA  
1451  CCTTAGGTCA CTGGAAAGAT GTCGAGCGGA TCGCTCACAA CCAGTCGGTA  
1501  GATGTCAAGA AGAGACGTTG GGTTACCTTC TGCTCTGCAG AATGGCCAAC  
1551  CTTTAACGTC GGATGGCCGC GAGACGGCAC CTTTAACCGA GACCTCATCA  
1601  CCCAGGTTAA GATCAAGGTC TTTTCACCTG GCCCGCATGG ACACCCAGAC  
1651  CAGGTCCCCT ACATCGTGAC CTGGGAAGCC TTGGCTTTTG ACCCCCCTCC  
1701  CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT CTTCCTCCAT  
1751  CCGCCCCGTC TCTCCCCCTT GAACCTCCTC GTTCGACCCC GCCTCGATCC  
1801  TCCCTTTATC CAGCCCTCAC TCCTTCTCTA GGCGCCCCCA TATGGCCATA  
1851  TGAGATCTTA TATGGGGCAC CCCCGCCCCT TGTAAACTTC CCTGACCCTG  
1901  ACATGACAAG AGTTACTAAC AGCCCCTCTC TCCAAGCTCA CTTACAGGCT  
1951  CTCTACTTAG TCCAGCACGA AGTCTGGAGA CCTCTGGCGG CACGTACCAA  
2001  GAACAACTGG ACCGACCGGT GGTACCTCAC CCTTACCGAG TCGGCGACAC  
2051  AGTGTGGGTC CGCCGACACC AGACTAAGAA CCTAGAACCT CGCTGGAAAG  
2101  GACCTTACAC AGTCCTGCTG ACCACCCCCA CCGCCCTCAA AGTAGACGGC  
2151  ATCGCAGCTT GGATACACGC CGCCCACGTG AAGGCTGCCG ACCCCGGGGG  
2201  TGGACCATCC TCTAGACTGC CGGATCGAAT TGTCGACGAA TTCGGATCCT  
2251  CGAGCGGCCG CGATCCGAGT TCTTCTGAGC GGGACTCTGG GGTTCGATAA  
2301  AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT GAAAGACCCC  
2351  ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG  
2401  GAAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TCAGGAACAG  
2451  ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC  
2501  TGCCCCGGCT CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA  
2551  CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG  
2601  ATGGTCCCCA GATGCGGTCC AGCCCTCAGC AGTTTCTAGA GAACCATCAG  
2651  ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG CCTTATTTGA  
2701  ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC  
2751  GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGGGCGC CAGTCCTCCG  
2801  ATTGACTGAG TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT  
2851  TGCATCCGAC TTGTGGTCTC GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG  
2901  ATTGACTACC CGTCAGCGGG GGTCTTTCAT TTGGGGGCTC GTCCGGGATC  
2951  GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA GGTAAGCTGG  
3001  CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC  
3051  TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA  
3101  GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT  
3151  GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC  
3201  ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC  
3251  ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG  
3301  CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC  
3351  TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG  
3401  GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG  
3451  GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA  
3501  CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA  
3551  GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG  
3601  ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT  
3651  GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG  
3701  TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC  
3751  TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA  
3801  CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT  
3851  ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC  
3901  ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT  
3951  CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA  
4001  GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA  
4051  TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA  
4101  CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT  
4151  TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT  
4201  ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC  
4251  ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC  
4301  CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT  
4351  GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC  
4401  AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT  
4451  TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT  
4501  AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGGCAT  
4551  CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC  
4601  AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT  
4651  AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT  
4701  ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT  
4751  CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA  
4801  GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAACACGGGA  
4851  TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC  
4901  GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT  
4951  TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT  
5001  CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA  
5051  AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT  
5101  CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT  
5151  ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC  
5201  CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA  
5251  ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AA

载体质粒图谱pdf版和相关资料下载

载体应用举例

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