pBABE-Puro

价格:1200元

联系方式:I47-825O-882O

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pBABEpuro载体基本信息

出品公司: Addgene
载体名称: pBABE-puro
质粒类型: 逆病毒载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: MSCV
载体大小: 5177 bp
5' 测序引物及序列: pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'
3' 测序引物及序列: pBABE 3' primer 5'ACCCTAACTGACACACATTCC3'
载体标签:
载体抗性: 氨苄青霉素
筛选标记: Puromycin
克隆菌株: DH5α、HB101等
宿主细胞(系): 常规哺乳动物细胞系
备注:
推荐使用逆病毒包装载体:pUMVCpCMV-VSV-G
 
产品目录号: #1764
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

pBABEpuro载体质粒图谱和多克隆位点信息

pBABE-puro载体图谱



pBABE-puro多克隆位点

pBABEpuro载体简介

pBABEpuro载体序列

LOCUS       pBABE-puro	5169 bp 	DNA     circular	SYN
DEFINITION  pBABE-puro
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="pBABE-puro"
                     /mol_type="other DNA"
     misc_feature    12..479
                     /label="5_LTR2"
     misc_feature    18..479
                     /label="3MoMuLV_LTR"
     misc_feature    549..1350
                     /label="psi_plus_pack"
     misc_feature    1318..1334
                     /label="pBABE_5_primer"
     misc_feature    complement(1408..1428)
                     /label="pBABE_3_primer"
     misc_feature    complement(1414..1629)
                     /label="SV40_enhancer"
     promoter        1426..1694
                     /label="SV40_promoter"
     rep_origin      1593..1670
                     /label="SV40_origin"
     misc_feature    1655..1674
                     /label="SV40pro_F_primer"
     CDS             complement(1728..2402)
                     /label="ORF frame 2"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGRAVLRALDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLFAKA*"
     gene            1752..2351
                     /label="puro (variant)"
                     /gene="puro (variant)"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGRAVLRALDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLFAKA*"
     CDS             1752..2351
                     /label="ORF frame 3"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
     misc_feature    2450..3043
                     /label="3MoMuLV_LTR"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
     misc_feature    2492..3043
                     /label="5_LTR2"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
     gene            complement(4181..5041)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"
     CDS             complement(4181..5041)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5083..5111)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
   61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
  121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
  181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
  241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
  301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
  361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
  421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
  481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
  541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
  601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
  661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
  721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
  781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
  841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
  901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
  961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
 1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
 1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
 1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
 1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
 1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
 1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga
 1381 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc
 1441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
 1501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
 1561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
 1621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
 1681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
 1741 aaaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
 1801 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
 1861 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
 1921 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
 1981 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
 2041 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
 2101 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
 2161 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
 2221 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
 2281 ccgtcaccgc cgacgtcgag tgcccgaagg accgcgcgac ctggtgcatg acccgcaagc
 2341 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
 2401 atgcatcgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
 2461 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
 2521 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
 2581 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
 2641 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
 2701 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
 2761 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
 2821 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
 2881 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
 2941 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
 3001 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttccgact tgtggtctcg
 3061 ctgccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt cttcacatgc
 3121 agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt
 3181 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc gctcttccgc
 3241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
 3301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
 3361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
 3421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
 3481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
 3541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
 3601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
 3661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
 3721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
 3781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
 3841 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
 3901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
 3961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
 4021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
 4081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt tgcggccgca
 4141 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
 4201 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
 4261 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
 4321 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
 4381 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
 4441 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
 4501 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
 4561 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
 4621 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
 4681 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
 4741 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
 4801 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
 4861 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
 4921 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
 4981 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
 5041 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
 5101 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
 5161 aagtgccac
//

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