pPRIME-TET-GFP-FF3

价格:1000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: pPRIME-TET-GFP-FF3
质粒类型: 慢病毒载体;RNAi载体;四环素诱导载体
高拷贝/低拷贝: 低拷贝
启动子: TET
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8226bp
5' 测序引物及序列: EGFP-C Forward: CATGGTCCTGCTGGAGTTCGTG
3' 测序引物及序列: --
载体标签: GFP
载体抗性: 氨苄青霉素
筛选标记: 氯霉素
备注: 克隆宿主菌为Stbl3,在30度条件下进行质粒扩增。
产品目录号: 11662
稳定性: 稳表达
组成型: 诱导型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pPRIME-TET-GFP-FF3载体图谱

载体简介

载体序列

LOCUS       pPRIME-TET-GFP-FF3	8226 bp 	DNA	SYN
DEFINITION  pPRIME-TET-GFP-FF3
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="pPRIME-TET-GFP-FF3"
                     /mol_type="other DNA"
     promoter        239..803
                     /label="CMV_immearly_promoter"
     misc_feature    318..605
                     /label="CAG_enhancer"
     misc_feature    772..792
                     /label="CMV_fwd_primer"
     misc_feature    820..976
                     /label="HIV-1_5_LTR"
     misc_feature    820..976
                     /label="truncHIV-1_3_LTR"
     misc_feature    1087..1131
                     /label="HIV-1_psi_pack"
     CDS             1523..2410
                     /label="ORF frame 2"

     misc_feature    1645..1878
                     /label="RRE"

     misc_feature    2409..2424
                     /label="cPPT"

     CDS             2663..3700
                     /label="ORF frame 2"

     misc_feature    2882..2901
                     /label="pCEP_fwd_primer"

     misc_feature    2884..2908
                     /label="LNCX_primer"

     CDS             2928..3647
                     /label="ORF frame 3"

     gene            2931..3644
                     /label="EGFP"
                     /gene="EGFP"

     misc_feature    complement(2973..2994)
                     /label="EGFP_N_primer"

     misc_feature    3111..3140
                     /label="Y66 (EGFP)"

     misc_feature    3581..3602
                     /label="EGFP_C_primer"

     gene            4172..4831
                     /label="CAT/CamR"
                     /gene="CAT/CamR"

     CDS             4172..4831
                     /label="ORF frame 2"

     misc_feature    5117..5704
                     /label="WPRE"

     CDS             5205..5759
                     /label="ORF frame 3"

     CDS             5218..6258
                     /label="ORF frame 1"

     misc_feature    complement(5707..5723)
                     /label="pBluescriptKS_primer"

     misc_feature    5895..5910
                     /label="cPPT"

     misc_feature    5895..5916
                     /label="U3PPT"

     misc_feature    6232..6412
                     /label="HIV-1_5_LTR"

     misc_feature    6232..6412
                     /label="truncHIV-1_3_LTR"

     gene            complement(7233..8093)
                     /label="Ampicillin"
                     /gene="Ampicillin"

     CDS             complement(7233..8093)
                     /label="ORF frame 2"

     promoter        complement(8135..8163)
                     /label="AmpR_promoter"

ORIGIN
    1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
   61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
  121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
  181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
  241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
  301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
  361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
  421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
  481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
  541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
  601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
  661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
  721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
  781 gcggtaggcg tgtacggtgg gaggtctata gaccagatct gagcctggga gctctctggc
  841 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
  901 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
  961 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
 1021 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
 1081 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
 1141 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
 1201 cagggggaaa gaaaaaatat aaataaacat atagtatggg caagcaggga gctagaacga
 1261 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
 1321 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
 1381 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
 1441 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat
 1501 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
 1561 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
 1621 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
 1681 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
 1741 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
 1801 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
 1861 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
 1921 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
 1981 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
 2041 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
 2101 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
 2161 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
 2221 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
 2281 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
 2341 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
 2401 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
 2461 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
 2521 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga
 2581 cctttcgtct tcattcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
 2641 tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat
 2701 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
 2761 tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc
 2821 cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag
 2881 cagagctcgt ttagtgaacc gtcagatcgc ctggaccggt cgccaccatg gtgagcaagg
 2941 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
 3001 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
 3061 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
 3121 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
 3181 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
 3241 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
 3301 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
 3361 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
 3421 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
 3481 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
 3541 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
 3601 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtagcgg ccgcaagcct
 3661 tgttaagtgc tcgcttcggc agcacatata ctatgtttga atgaggcttc agtactttac
 3721 agaatcgttg cctgcacatc ttggaaacac ttgctgggat tacttcttca ggttaaccca
 3781 acagaaggct cgagcaacca gaattcaagg ggctacttta ggagcaatta tcttgtttac
 3841 taaaactgaa taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta
 3901 taaattaaat cacttttttc aattggaaga ctaatgcgtt taaacacgcg gcgacgcgtt
 3961 cgaccgaata aaacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc
 4021 tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt
 4081 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
 4141 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
 4201 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
 4261 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
 4321 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
 4381 tccggaatta cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
 4441 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
 4501 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
 4561 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
 4621 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
 4681 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
 4741 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
 4801 gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta
 4861 aacgcctggt tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgga
 4921 tctcgaccgc gtttgggcgg tggctccctg ccacgcggct ccgaacagaa gctgatctcc
 4981 gaagaggatc tgacatgtgt ttaaacctcg acttaattaa gtcgagggtc gacggtatcg
 5041 ataagctcgc ttcacgagat catgtttaag ggttccggtt ccactaggta caattcgata
 5101 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
 5161 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
 5221 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
 5281 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
 5341 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
 5401 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
 5461 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
 5521 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
 5581 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
 5641 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
 5701 cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc
 5761 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
 5821 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
 5881 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
 5941 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
 6001 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
 6061 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
 6121 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
 6181 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
 6241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
 6301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
 6361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatgtga
 6421 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
 6481 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
 6541 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
 6601 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
 6661 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
 6721 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
 6781 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
 6841 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
 6901 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
 6961 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
 7021 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
 7081 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
 7141 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
 7201 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
 7261 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
 7321 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
 7381 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
 7441 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
 7501 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
 7561 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
 7621 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
 7681 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
 7741 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
 7801 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
 7861 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
 7921 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
 7981 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
 8041 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
 8101 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
 8161 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
 8221 cctgac
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng