pLVX-TRE3G-Luc Control

价格:3800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-TRE3G-Luc Control , pLVX TRE3G Luc Contrtol
质粒类型: 哺乳动物细胞慢病毒表达载体, 阳性对照质粒
高拷贝/低拷贝: 高拷贝
启动子: TRE3GV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 9452 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄
筛选标记: 嘌呤霉素
备注: 包含有Tet-On 3G反式激活蛋白,多西环素(doxycycline)诱导基因表达的慢病毒载体,是慢病毒表达的阳性对照质粒。
产品目录号: 631187
稳定性: /
组成型: 诱导型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-TRE3G-Luc Control载体图谱




pLVX-TRE3G-Luc Control载体特征

载体简介

载体序列

LOCUS       	pLVX-TRE3G-Luc Control   9452 bp    DNA   circular            31-MAY-2011
COMMENT     Created by Clontech Laboratories Inc.
COMMENT     631191,631193,631187, 631189, 631191, 631193, 631360, 631361,631362,631187,631189,631349,631350,631351,631352,631353,631354,631355,631356,631357, 635087
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     misc_feature    1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    1303..1536
                     /label=RRE
     misc_feature    2028..2151
                     /label=cPPT
     misc_feature    4233..4736
                     /label=pPGK
     misc_feature    4757..5356
                     /label=Puro(R)
     misc_feature    5374..5965
                     /label=WPRE
     3'clip          6169..6805
                     /label=3'\LTR
     misc_feature    complement(7275..7945)
                     /label=pUC\ori
     misc_feature    2566..4217
                     /label=Luciferase
     misc_feature    2210..2555
                     /label=pTRE3G
     misc_feature    complement(8090..8950)
                     /label=Amp(R)
BASE COUNT     2452 a      2295 c      2424 g      2281 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat 
     2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac 
     2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga 
     2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat 
     2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag 
     2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc 
     2521 ctggagcaat tccacaacac ttttgtctta tacttggatc caccatggaa gacgccaaaa 
     2581 acataaagaa aggcccggcg ccattctatc cgctagagga tggaaccgct ggagagcaac 
     2641 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac 
     2701 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag 
     2761 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc 
     2821 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga 
     2881 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag 
     2941 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa 
     3001 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca 
     3061 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct 
     3121 ttgatcgtga caaaacaatt gcactgataa tgaactcctc tggatctact gggttaccta 
     3181 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta 
     3241 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg 
     3301 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt 
     3361 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt 
     3421 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt 
     3481 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag 
     3541 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta 
     3601 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg 
     3661 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc 
     3721 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg 
     3781 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact 
     3841 gggacgaaga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag 
     3901 gataccaggt ggcccccgct gaattggagt cgatattgtt acaacacccc aacatcttcg 
     3961 acgcgggcgt ggcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg 
     4021 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag 
     4081 taacaaccgc caaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc 
     4141 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg 
     4201 gaaagtccaa attgtaaaat acgcgtgaat tctaccgggt aggggaggcg cttttcccaa 
     4261 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc 
     4321 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg 
     4381 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc 
     4441 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg 
     4501 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt 
     4561 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg 
     4621 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc 
     4681 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct 
     4741 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 
     4801 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 
     4861 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 
     4921 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 
     4981 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 
     5041 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 
     5101 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 
     5161 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 
     5221 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 
     5281 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 
     5341 caagcccggt gcctgacggg gcgcgtctgg aacaatcaac ctctggatta caaaatttgt 
     5401 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 
     5461 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 
     5521 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 
     5581 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 
     5641 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 
     5701 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 
     5761 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 
     5821 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 
     5881 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 
     5941 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg 
     6001 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac 
     6061 aagaggagga ggaggtgggt ttttccagtc acacctcagg tacctttaag accaatgact 
     6121 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta 
     6181 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac 
     6241 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga 
     6301 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag 
     6361 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg 
     6421 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg 
     6481 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt 
     6541 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa 
     6601 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct 
     6661 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 
     6721 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 
     6781 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 
     6841 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt 
     6901 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 
     6961 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 
     7021 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 
     7081 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 
     7141 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 
     7201 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 
     7261 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 
     7321 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
     7381 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 
     7441 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
     7501 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 
     7561 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 
     7621 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
     7681 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 
     7741 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 
     7801 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 
     7861 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 
     7921 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 
     7981 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 
     8041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 
     8101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 
     8161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 
     8221 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 
     8281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 
     8341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 
     8401 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 
     8461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 
     8521 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 
     8581 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 
     8641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 
     8701 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 
     8761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 
     8821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 
     8881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 
     8941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 
     9001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 
     9061 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct 
     9121 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 
     9181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac 
     9241 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact 
     9301 gccaattacc tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt 
     9361 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt 
     9421 atttgttaaa tatgtactac aaacttagta gt 
//

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