FUW-tetO-hSOX2

价格:900元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: FUW-tetO-hSOX2
质粒类型: 慢病毒载体;四环素调控载体;Tet-On系统载体
高拷贝/低拷贝: 高拷贝
克隆方法: EcoRI
启动子: TRE/Minimal CMV
载体大小: 9348 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: bleomycin, phleomycin, and Zeocin?
克隆菌株: Stbl3
宿主细胞(系): 大部分哺乳动物细胞
备注: FUW-tetO-hSOX2 vector expressing the reverse tetracycline transactivator
产品目录号: #20724
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

FUW-tetO-hSOX2载体图谱



载体简介

载体序列

LOCUS       FUW-tetO-hSOX2	9348 bp 	DNA	SYN
DEFINITION  FUW-tetO-hSOX2
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="FUW-tetO-hSOX2"
                     /mol_type="other DNA"
     promoter        8..584
                     /label="CMV_immearly_promoter"
     misc_feature    87..374
                     /label="CAG_enhancer"
     misc_feature    541..561
                     /label="CMV_fwd_primer"
     misc_feature    604..784
                     /label="HIV-1_5_LTR"
     misc_feature    604..784
                     /label="truncHIV-1_3_LTR"
     misc_feature    895..939
                     /label="HIV-1_psi_pack"
     CDS             1327..2214
                     /label="ORF frame 1"
                     
     misc_feature    1449..1682
                     /label="RRE"
                    
     misc_feature    2213..2228
                     /label="cPPT"
                    
     misc_feature    2371..2688
                     /label="TRE"
                     
     promoter        2689..2808
                     /label="CMV2_promoter"
                    
     misc_feature    2720..2739
                     /label="pCEP_fwd_primer"
                    
     misc_feature    2722..2746
                     /label="LNCX_primer"
                    
     CDS             2831..3784
                     /label="ORF frame 2"
                    
     misc_feature    3811..4398
                     /label="WPRE"
                   
     CDS             3899..4948
                     /label="ORF frame 2"
                     
     CDS             3912..4424
                     /label="ORF frame 3"
                    
     misc_feature    complement(4401..4417)
                     /label="pBluescriptKS_primer"
                    
     misc_feature    4585..4606
                     /label="U3PPT"
                    
     misc_feature    4585..4600
                     /label="cPPT"
                     
     misc_feature    4922..5102
                     /label="truncHIV-1_3_LTR"
                    
     misc_feature    4922..5102
                     /label="HIV-1_5_LTR"
                    
     misc_feature    complement(5128..5145)
                     /label="BGH_rev_primer"
                    
     terminator      5131..5358
                     /label="bGH_PA_terminator"
                     
     rep_origin      5421..5727
                     /label="f1_origin"
                    
     misc_feature    complement(5841..5861)
                     /label="pBABE_3_primer"
                    
     misc_feature    complement(5847..6062)
                     /label="SV40_enhancer"
                  
     promoter        5859..6127
                     /label="SV40_promoter"
                   
     rep_origin      6026..6103
                     /label="SV40_origin"
                    
     misc_feature    6088..6107
                     /label="SV40pro_F_primer"
                     
     promoter        6221..6288
                     /label="EM7_promoter"
     
     gene            6289..6660
                     /label="bleo"
                     /gene="bleo"
    
     misc_feature    6289..6663
                     /label="sh_ble"
          
     terminator      6796..6915
                     /label="SV40_PA_terminator"
         
     misc_feature    6884..6903
                     /label="EBV_rev_primer"

     promoter        complement(6959..6977)
                     /label="M13_reverse_primer"

     misc_feature    complement(6976..6998)
                     /label="M13_pUC_rev_primer"

     promoter        complement(7012..7041)
                     /label="lac_promoter"

     gene            complement(8124..8984)
                     /label="Ampicillin"
                     /gene="Ampicillin"

     CDS             complement(8124..8984)
                     /label="ORF frame 2"

     promoter        complement(9026..9054)
                     /label="AmpR_promoter"

ORIGIN
    1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
   61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
  121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
  181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
  241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
  301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
  361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
  421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
  481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
  541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
  601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
  661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
  721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
  781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
  841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
  901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
  961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
 1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
 1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
 1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
 1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
 1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
 1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
 1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
 1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
 1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
 1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
 1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
 1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
 1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
 1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
 1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
 1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
 1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
 2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
 2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
 2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
 2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
 2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
 2341 ggacagcaga gatccagttt ggttaatgat ctcgagttta ccactcccta tcagtgatag
 2401 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
 2461 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
 2521 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
 2581 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
 2641 tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgagta ggcgtgtacg
 2701 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
 2761 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga
 2821 attcgccacc atgtacaaca tgatggagac ggagctgaag ccgccgggcc cgcagcaaac
 2881 ttcggggggc ggcggcggca actccaccgc ggcggcggcc ggcggcaacc agaaaaacag
 2941 cccggaccgc gtcaagcggc ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg
 3001 caagatggcc caggagaacc ccaagatgca caactcggag atcagcaagc gcctgggcgc
 3061 cgagtggaaa cttttgtcgg agacggagaa gcggccgttc atcgacgagg ctaagcggct
 3121 gcgagcgctg cacatgaagg agcacccgga ttataaatac cggccccggc ggaaaaccaa
 3181 gacgctcatg aagaaggata agtacacgct gcccggcggg ctgctggccc ccggcggcaa
 3241 tagcatggcg agcggggtcg gggtgggcgc cggcctgggc gcgggcgtga accagcgcat
 3301 ggacagttac gcgcacatga acggctggag caacggcagc tacagcatga tgcaggacca
 3361 gctgggctac ccgcagcacc cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat
 3421 gcaccgctac gacgtgagcg ccctgcagta caactccatg accagctcgc agacctacat
 3481 gaacggctcg cccacctaca gcatgtccta ctcgcagcag ggcacccctg gcatggctct
 3541 tggctccatg ggttcggtgg tcaagtccga ggccagctcc agcccccctg tggttacctc
 3601 ttcctcccac tccagggcgc cctgccaggc cggggacctc cgggacatga tcagcatgta
 3661 tctccccggc gccgaggtgc cggaacccgc cgcccccagc agacttcaca tgtcccagca
 3721 ctaccagagc ggcccggtgc ccggcacggc cattaacggc acactgcccc tctcacacat
 3781 gtgagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
 3841 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
 3901 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
 3961 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
 4021 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
 4081 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
 4141 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
 4201 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
 4261 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
 4321 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
 4381 ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat
 4441 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
 4501 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
 4561 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
 4621 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
 4681 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
 4741 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
 4801 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
 4861 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
 4921 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
 4981 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
 5041 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
 5101 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
 5161 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
 5221 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
 5281 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
 5341 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
 5401 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
 5461 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
 5521 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
 5581 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
 5641 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
 5701 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
 5761 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
 5821 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
 5881 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
 5941 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
 6001 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
 6061 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
 6121 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
 6181 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
 6241 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
 6301 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
 6361 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
 6421 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
 6481 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
 6541 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
 6601 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
 6661 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
 6721 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
 6781 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
 6841 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
 6901 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
 6961 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
 7021 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
 7081 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
 7141 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
 7201 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
 7261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
 7321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
 7381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
 7441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
 7501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
 7561 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
 7621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
 7681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
 7741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
 7801 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
 7861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
 7921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
 7981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
 8041 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
 8101 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
 8161 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
 8221 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
 8281 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
 8341 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
 8401 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
 8461 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
 8521 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
 8581 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
 8641 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
 8701 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
 8761 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
 8821 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
 8881 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
 8941 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
 9001 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
 9061 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
 9121 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
 9181 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
 9241 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
 9301 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatata
//

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