pYES3/CT

价格:1000元

联系方式:I47-825O-882O

买家导航

pYES3-CT载体基本信息

出品公司: Invitrogen
载体名称: pYES3-CT, pYES3/CT
质粒类型: 酿酒酵母蛋白表达载体
表达水平: 高拷贝
诱导方法: 半乳糖
启动子: GAL1
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5870 bp
5' 测序引物及序列: GAL1-F: AATATACCTCTATACTTTAACGTC
3' 测序引物及序列: CYC1-R: GCGTGAATGTAAGCGTGAC
载体标签: C-His, C-V5
载体抗性: 氨苄
筛选标记: TRP1
备注: 利用半乳糖诱导蛋白在酿酒酵母中表达。
产品目录号: V8253-20
稳定性: 稳定 Stable
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

pYES3-CT载体质粒图谱和多克隆位点信息

pYES3/CT载体图谱

pYES3/CT多克隆位点

pYES3-CT载体简介

pYES3-CT载体序列

LOCUS       pYES3/CT	5870 bp 	DNA     circular	SYN
DEFINITION  pYES3/CT
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="pYES3/CT"
                     /mol_type="other DNA"
     promoter        1..451
                     /label="GAL1_promoter"
     misc_feature    414..437
                     /label="GAL1_primer"
     promoter        475..493
                     /label="T7_promoter"
     terminator      716..961
                     /label="CYC1_terminator"
     misc_feature    complement(725..743)
                     /label="CYC1_primer"
     misc_feature    complement(725..743)
                     /label="pYESTrp_rev_primer"
     gene            complement(1963..2823)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(1963..2823)
                     /label="ORF frame 3"
     promoter        complement(2865..2893)
                     /label="AmpR_promoter"
     gene            3133..3807
                     /label="TRP1"
                     /gene="TRP1"
     CDS             3133..3807
                     /label="ORF frame 1"
     rep_origin      3859..5330
                     /label="2micron_origin"
ORIGIN
    1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
   61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
  121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
  181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
  241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
  301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
  361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
  421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
  481 GACTCACTAT AGGGAATATT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT AACGGCCGCC
  541 AGTGTGCTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC
  601 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
  661 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCCTAGA GGGCCGCATC ATGTAATTAG
  721 TTATGTCACG CTTACATTCA CGCCCTCCCC CCACATCCGC TCTAACCGAA AAGGAAGGAG
  781 TTAGACAACC TGAAGTCTAG GTCCCTATTT ATTTTTTTAT AGTTATGTTA GTATTAAGAA
  841 CGTTATTTAT ATTTCAAATT TTTCTTTTTT TTCTGTACAG ACGCGTGTAC GCATGTAACA
  901 TTATACTGAA AACCTTGCTT GAGAAGGTTT TGGGACGCTC GAAGGCTTTA ATTTGCAAGC
  961 TGCGGCCCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
 1021 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
 1081 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
 1141 GAACATGTGA GCAAAAGGCC AGCAAAAGCC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
 1201 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
 1261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
 1321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
 1381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
 1441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
 1501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
 1561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
 1621 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
 1681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
 1741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
 1801 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
 1861 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
 1921 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
 1981 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
 2041 CGTGTAGATA ACTACGATAC GGGAGCGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
 2101 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
 2161 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
 2221 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC
 2281 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
 2341 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
 2401 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
 2461 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
 2521 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAC
 2581 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
 2641 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
 2701 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
 2761 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
 2821 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG
 2881 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG
 2941 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG
 3001 GCGTATCACG AGGCCCTTTC GTCTTCAAGA AATTCGGTCG AAAAAAGAAA AGGAGAGGGC
 3061 CAAGAGGGAG GGCATTGGTG ACTATTGAGC ACGTGAGTAT ACGTGATTAA GCACACAAAG
 3121 GCAGCTTGGA GTATGTCTGT TATTAATTTC ACAGGTAGTT CTGGTCCATT GGTGAAAGTT
 3181 TGCGGCTTGC AGAGCACAGA GGCCGCAGAA TGTGCTCTAG ATTCCGATGC TGACTTGCTG
 3241 GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC CGGTTATTGC AAGGAAAATT
 3301 TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC CGAAATACTT GGTTGGCGTG
 3361 TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA ATGATTACGG CATTGATATC
 3421 GTCCAACTGC ACGGAGATGA GTCGTGGCAA GAATACCAAG AGTTCCTCGG TTTGCCAGTT
 3481 ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC TCAGTGCAGC TTCACAGAAA
 3541 CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG GGACAGGTGA ACTTTTGGAT
 3601 TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC CCGAGAGCTT ACATTTTATG
 3661 TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC TTAGATTAAA TGGCGTTATT
 3721 GGTGTTGATG TAAGCGGAGG TGTGGAGACA AATGGTGTAA AAGACTCTAA CAAAATAGCA
 3781 AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT AGTATTTATT TAAGTATTGT
 3841 TTGTGCACTT GCCCTAGCTT ATCGATGATA AGCTGTCAAA GATGAGAATT AATTCCACGG
 3901 ACTATAGACT ATACTAGATA CTCCGTCTAC TGTACGATAC ACTTCCGCTC AGGTCCTTGT
 3961 CCTTTAACGA GGCCTTACCA CTCTTTTGTT ACTCTATTGA TCCAGCTCAG CAAAGGCAGT
 4021 GTGATCTAAG ATTCTATCTT CGCGATGTAG TAAAACTAGC TAGACCGAGA AAGAGACTAG
 4081 AAATGCAAAA GGCACTTCTA CAATGGCTGC CATCATTATT ATCCGATGTG ACGCTGCAGC
 4141 TTCTCAATGA TATTCGAATA CGCTTTGAGG AGATACAGCC TAATATCCGA CAAACTGTTT
 4201 TACAGATTTA CGATCGTACT TGTTACCCAT CATTGAATTT TGAACATCCG AACCTGGGAG
 4261 TTTTCCCTGA AACAGATAGT ATATTTGAAC CTGTATAATA ATATATAGTC TAGCGCTTTA
 4321 CGGAAGACAA TGTATGTATT TCGGTTCCTG GAGAAACTAT TGCATCTATT GCATAGGTAA
 4381 TCTTGCACGT CGCATCCCCG GTTCATTTTC TGCGTTTCCA TCTTGCACTT CAATAGCATA
 4441 TCTTTGTTAA CGAAGCATCT GTGCTTCATT TTGTAGAACA AAAATGCAAC GCGAGAGCGC
 4501 TAATTTTTCA AACAAAGAAT CTGAGCTGCA TTTTTACAGA ACAGAAATGC AACGCGAAAG
 4561 CGCTATTTTA CCAACGAAGA ATCTGTGCTT CATTTTTGTA AAACAAAAAT GCAACGCGAC
 4621 GAGAGCGCTA ATTTTTCAAA CAAAGAATCT GAGCTGCATT TTTACAGAAC AGAAATGCAA
 4681 CGCGAGAGCG CTATTTTACC AACAAAGAAT CTATACTTCT TTTTTGTTCT ACAAAAATGC
 4741 ATCCCGAGAG CGCTATTTTT CTAACAAAGC ATCTTAGATT ACTTTTTTTC TCCTTTGTGC
 4801 GCTCTATAAT GCAGTCTCTT GATAACTTTT TGCACTGTAG GTCCGTTAAG GTTAGAAGAA
 4861 GGCTACTTTG GTGTCTATTT TCTCTTCCAT AAAAAAAGCC TGACTCCACT TCCCGCGTTT
 4921 ACTGATTACT AGCGAAGCTG CGGGTGCATT TTTTCAAGAT AAAGGCATCC CCGATTATAT
 4981 TCTATACCGA TGTGGATTGC GCATACTTTG TGAACAGAAA GTGATAGCGT TGATGATTCT
 5041 TCATTGGTCA GAAAATTATG AACGGTTTCT TCTATTTTGT CTCTATATAC TACGTATAGG
 5101 AAATGTTTAC ATTTTCGTAT TGTTTTCGAT TCACTCTATG AATAGTTCTT ACTACAATTT
 5161 TTTTGTCTAA AGAGTAATAC TAGAGATAAA CATAAAAAAT GTAGAGGTCG AGTTTAGATG
 5221 CAAGTTCAAG GAGCGAAAGG TGGATGGGTA GGTTATATAG GGATATAGCA CAGAGATATA
 5281 TAGCAAAGAG ATACTTTTGA GCAATGTTTG TGGAAGCGGT ATTCGCAATG GGAAGCTCCA
 5341 CCCCGGTTGA TAATCAGAAA AGCCCCAAAA ACAGGAAGAT TGTATAAGCA AATATTTAAA
 5401 TTGTAAACGT TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT
 5461 TTAACGAATA GCCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG
 5521 GGTTGAGTGT TGTTCCAGTT TCCAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG
 5581 TCAAAGGGCG AAAAAGGGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT
 5641 CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CAGTAAATCG GAAGGGTAAA CGGATGCCCC
 5701 CATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA
 5761 AAGGAGCGGG GGCTAGGGCG GTGGGAAGTG TAGGGGTCAC GCTGGGCGTA ACCACCACAC
 5821 CCGCCGCGCT TAATGGGGCG CTACAGGGCG CGTGGGGATG ATCCACTAGT
//

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pYES3-CT载体应用举例

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