pcDNA3.1+N-6HIS

LOCUS       pcDNA3.1+N-6His        5455 bp    DNA     circular SYN 27-AUG-2021
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14017131|
COMMENT     VNTDBDATE|-14017131|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pcDNA3.1+N-6His|
COMMENT     VNTAUTHORNAME|Biofeng.com|
COMMENT     VNTAUTHOREML|order@biofeng.com|
COMMENT     VNTOAUTHORNAME|Invitrogen|
COMMENT     VNTOAUTHORTEL|(800)-357-3114|
COMMENT     VNTOAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTOAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     source          1..5455
                     /invitrogen="124"
                     /vntifkey="98"
     CDS             complement(4459..5319)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     polyA_site      1055..1279
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     primer          complement(1049..1066)
                     /invitrogen="1910000"
                     /vntifkey="27"
                     /label=BGH\reverse\primer
     promoter        complement(5320..5418)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     primer          769..789
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     promoter        232..819
                     /invitrogen="2180000"
                     /vntifkey="29"
                     /label=CMV\promoter
     rep_origin      1325..1753
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     misc_feature    944..1037
                     /invitrogen="1630000"
                     /vntifkey="21"
                     /label=MCS
     CDS             2163..2957
                     /invitrogen="1260000"
                     /vntifkey="4"
                     /label=Neo(R)
     rep_origin      complement(3644..4314)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        1758..2127
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    3131..3261
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     primer          863..882
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     promoter        863..879
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    917..922
                     /vntifkey="21"
                     /label=Kozak\sequence
     misc_feature    926..943
                     /vntifkey="21"
                     /label=His\Tag
BASE COUNT     1259 a      1423 c      1388 g      1385 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttgcca ccatgcatca tcaccatcac catggtaccg agctcggatc 
      961 cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct 
     1021 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 
     1081 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 
     1141 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 
     1201 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 
     1261 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat 
     1321 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 
     1381 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 
     1441 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 
     1501 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 
     1561 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 
     1621 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 
     1681 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 
     1741 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 
     1801 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 
     1861 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 
     1921 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 
     1981 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct 
     2041 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 
     2101 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc 
     2161 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 
     2221 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 
     2281 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 
     2341 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 
     2401 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 
     2461 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 
     2521 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 
     2581 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 
     2641 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg 
     2701 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 
     2761 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 
     2821 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 
     2881 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 
     2941 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa 
     3001 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 
     3061 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 
     3121 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
     3181 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 
     3241 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg 
     3301 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 
     3361 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 
     3421 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 
     3481 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
     3541 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
     3601 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
     3661 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
     3721 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
     3781 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
     3841 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
     3901 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
     3961 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
     4021 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
     4081 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
     4141 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
     4201 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca 
     4261 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 
     4321 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 
     4381 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 
     4441 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 
     4501 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 
     4561 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 
     4621 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 
     4681 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 
     4741 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 
     4801 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 
     4861 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 
     4921 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 
     4981 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 
     5041 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 
     5101 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 
     5161 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 
     5221 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 
     5281 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 
     5341 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 
     5401 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc 
//