| 质粒名称: | pCAG-T7-TALEN(Sangamo)-Destination |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 37184 |
| 存储实验室: | Pawel Pelczar |
| 用途: | 采用同源FokI域 |
|
载体骨架基本信息 |
|
| 载体名称: | pDIRECT |
| 载体出品公司: | CLONTECH |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4810 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 启动子 | CAG |
| 标签/融合蛋白: | FokI nuclease（C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | |
| 3' 克隆位点: | |
| 5' 测序引物 | TAL_F1 (ttggcgtcggcaaacagtgg) |
| 3' 测序引物 | TAL_R2 (ggcgacgaggtggtcgttg |
|
文献引用方法 |
|
| 材料和方法部分: | pCAG-T7-TALEN(Sangamo)-Destination was a gift from Pawel Pelczar (Addgene plasmid # 37184) |
| 参考文献部分: | Mouse genome engineering using designer nucleases. Hermann M, Cermak T, Voytas DF, Pelczar P. J Vis Exp. 2014 Apr 2;(86). doi: 10.3791/50930. 10.3791/50930 PubMed 24747757 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
-Destination载体图谱.png)
载体简介
载体序列
LOCUS pCAG-T7-TALEN(Sangamo)-Destination 6441 bp DNA SYN
DEFINITION pCAG-T7-TALEN(Sangamo)-Destination
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6441
/organism="pCAG-T7-TALEN(Sangamo)-Destination"
/mol_type="other DNA"
promoter 86..115
/label="lac_promoter"
misc_feature 129..151
/label="M13_pUC_rev_primer"
promoter 150..168
/label="M13_reverse_primer"
misc_feature 264..551
/label="CAG_enhancer"
CDS complement(983..1714)
/label="ORF frame 3"
CDS complement(1024..1527)
/label="ORF frame 1"
promoter 1864..1882
/label="T7_promoter"
CDS 1893..2501
/label="ORF frame 3"
misc_feature 1902..1922
/label="SV40 NLS"
misc_feature 1908..1922
/label="NLS"
misc_feature complement(2357..2512)
/label="lacZ_a"
misc_feature 2483..2505
/label="M13_pUC_fwd_primer"
promoter 2498..2514
/label="M13_forward20_primer"
promoter 2524..2542
/label="T7_promoter"
promoter complement(2589..2608)
/label="T3_promoter"
promoter complement(2625..2643)
/label="M13_reverse_primer"
misc_feature complement(2642..2664)
/label="M13_pUC_rev_primer"
promoter complement(2678..2707)
/label="lac_promoter"
CDS 2722..3519
/label="ORF frame 1"
misc_feature complement(3538..3555)
/label="BGH_rev_primer"
terminator 3541..3768
/label="bGH_PA_terminator"
CDS 3742..4215
/label="ORF frame 1"
promoter complement(3840..3856)
/label="M13_forward20_primer"
misc_feature complement(3849..3871)
/label="M13_pUC_fwd_primer"
misc_feature 3849..3997
/label="lacZ_a"
rep_origin 4015..4321
/label="f1_origin"
promoter 4514..4542
/label="AmpR_promoter"
gene 4584..5444
/label="Ampicillin"
/gene="Ampicillin"
CDS 4584..5444
/label="ORF frame 3"
rep_origin 5599..6218
/label="pBR322_origin"
ORIGIN
1 GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT
61 AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG
121 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAG GCGCGCCGGA
181 TTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA
241 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC
301 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG
361 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC
421 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG
481 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG
541 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA
601 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG
661 CGATGGGGGC GGGGGGGGGG GGGGGGCGCG CGCCAGGCGG GGCGGGGCGG GGCGAGGGGC
721 GGGGCGGGGC GAGGCGGAGA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT
781 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG
841 CGGGAGTCGC TGCGTTGCCT TCGCCCCGTG CCCCGCTCCG CGCCGCCTCG CGCCGCCCGC
901 CCCGGCTCTG ACTGACCGCG TTACTCCCAC AGGTGAGCGG GCGGGACGGC CCTTCTCCTC
961 CGGGCTGTAA TTAGCGCTTG GTTTAATGAC GGCTCGTTTC TTTTCTGTGG CTGCGTGAAA
1021 GCCTTAAAGG GCTCCGGGAG GGCCCTTTGT GCGGGGGGGA GCGGCTCGGG GGGTGCGTGC
1081 GTGTGTGTGT GCGTGGGGAG CGCCGCGTGC GGCCCGCGCT GCCCGGCGGC TGTGAGCGCT
1141 GCGGGCGCGG CGCGGGGCTT TGTGCGCTCC GCGTGTGCGC GAGGGGAGCG CGGCCGGGGG
1201 CGGTGCCCCG CGGTGCGGGG GGGCTGCGAG GGGAACAAAG GCTGCGTGCG GGGTGTGTGC
1261 GTGGGGGGGT GAGCAGGGGG TGTGGGCGCG GCGGTCGGGC TGTAACCCCC CCCTGCACCC
1321 CCCTCCCCGA GTTGCTGAGC ACGGCCCGGC TTCGGGTGCG GGGCTCCGTG CGGGGCGTGG
1381 CGCGGGGCTC GCCGTGCCGG GCGGGGGGTG GCGGCAGGTG GGGGTGCCGG GCGGGGCGGG
1441 GCCGCCTCGG GCCGGGGAGG GCTCGGGGGA GGGGCGCGGC GGCCCCGGAG CGCCGGCGGC
1501 TGTCGAGGCG CGGCGAGCCG CAGCCATTGC CTTTTATGGT AATCGTGCGA GAGGGCGCAG
1561 GGACTTCCTT TGTCCCAAAT CTGGCGGAGC CGAAATCTGG GAGGCGCCGC CGCACCCCCT
1621 CTAGCGGGCG CGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG GGGAGGGCCT
1681 TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCATCTCCAG CCTCGGGGCT GCCGCAGGGG
1741 GACGGCTGCC TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC
1801 GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GATCCTTAAT
1861 TAATAATACG ACTCACTATA GGGGCCGCCA CCATGAGATC TCCTAAGAAA AAGAGGAAGG
1921 TGCAGGTGGA TCTACGCACG CTCGGCTACA GTCAGCAGCA GCAAGAGAAG ATCAAACCGA
1981 AGGTGCGTTC GACAGTGGCG CAGCACCACG AGGCACTGGT GGGCCATGGG TTTACACACG
2041 CGCACATCGT TGCGCTCAGC CAACACCCGG CAGCGTTAGG GACCGTCGCT GTCACGTATC
2101 AGCACATAAT CACGGCGTTG CCAGAGGCGA CACACGAAGA CATCGTTGGC GTCGGCAAAC
2161 AGTGGTCCGG CGCACGCGCC CTGGAGGCCT TGCTCACGGA TGCGGGGGAG TTGAGAGGTC
2221 CGCCGTTACA GTTGGACACA GGCCAACTTG TGAAGATTGC AAAACGTGGC GGCGTGACCG
2281 CAATGGAGGC AGTGCATGCA TCGCGCAATG CACTGACGGG TGCCCCCCTG GAGACGGGCG
2341 CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG
2401 GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA
2461 AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC
2521 GCGTAATACG ACTCACTATA GGGCGAATTG GGTACCGGGC CCCCCCTCGA GGTCCTCCAG
2581 CTTTTGTTCC CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGT CATAGCTGTT
2641 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA
2701 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT
2761 GCCCGCTTTC CACCGGTCGT CTCCAACGAC CACCTCGTCG CCTTGGCCTG CCTCGGCGGA
2821 CGTCCTGCCA TGGATGCAGT GAAAAAGGGA TTGCCGCACG CGCCGGAATT GATCAGAAGA
2881 GTCAATCGCC GTATTGGCGA ACGCACGTCC CATCGCGTTg cggGATCcca gctagtgaaa
2941 tctgaattgg aaGAGAAGAA ATCTGAACTT AGACATAAAT TGAAATATGT GCCACATGAA
3001 TATATTGAAT TGATTGAAAT CGCAAGAAAT TCAACTCAGG ATAGAATCCT TGAAATGAAG
3061 GTGATGGAGT TCTTTATGAA GGTTTATGGT TATCGTGGTA AACATTTGGG TGGATCAAGG
3121 AAACCAGACG GAGCAATTTA TACTGTCGGA TCTCCTATTG ATTACGGTGT GATCGTTGAT
3181 ACTAAGGCAT ATTCAGGAGG TTATAATCTT CCAATTGGTC AAGCAGATGA AATGCAAAGA
3241 TATGTCGAAG AGAATCAAAC AAGAAACAAG CATATCAACC CTAATGAATG GTGGAAAGTC
3301 TATCCATCTT CAGTAACAGA ATTTAAGTTC TTGTTTGTGA GTGGTCATTT CAAAGGAAAC
3361 TACAAAGCTC AGCTTACAAG ATTGAATCAT ATCACTAATT GTAATGGAGC TGTTCTTAGT
3421 GTAGAAGAGC TTTTGATTGG TGGAGAAATG ATTAAAGCTG GTACATTGAC ACTTGAGGAA
3481 GTGAGAAGGA AATTTAATAA CGGTGAGATA AACTTTTAAT AGGAGCTCGC TGATCAGCCT
3541 CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA
3601 CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT
3661 GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG
3721 ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG
3781 AAAGAACCAG CTGGGGCTCG AGATCCACTA GTTCTAGCCT CGAGGCTAGA GCGGCCGCCA
3841 CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC
3901 CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC
3961 CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGACG CGCCCTGTAG CGGCGCATTA
4021 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG
4081 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA
4141 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC
4201 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT
4261 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA
4321 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC
4381 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA
4441 ACGCTTACAA TTTAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT
4501 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC
4561 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT
4621 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG
4681 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA
4741 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC
4801 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA
4861 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG
4921 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG
4981 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA
5041 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA
5101 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA
5161 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA
5221 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT
5281 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC
5341 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA
5401 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT
5461 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA
5521 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG
5581 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA
5641 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG
5701 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG
5761 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT
5821 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA
5881 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG
5941 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC
6001 GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA
6061 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC
6121 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT
6181 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
6241 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC
6301 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG
6361 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT
6421 GGCCGATTCA TTAATGCAGC T
//
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