pCAG-T7-TALEN(Sangamo)-Destination

价格:1200元

联系方式:I47-825O-882O

买家导航

载体基本信息

质粒名称: pCAG-T7-TALEN(Sangamo)-Destination 
出品公司: Addgene
目录编号: 37184
存储实验室: Pawel Pelczar
用途: 采用同源FokI域

载体骨架基本信息
载体名称: pDIRECT
载体出品公司: CLONTECH
载体抗性: 氨苄霉素
空载体大小: 4810 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
启动子 CAG
标签/融合蛋白: FokI nuclease(C端)
克隆方法: 限制性内切酶
5' 克隆位点:  
3' 克隆位点:  
5' 测序引物 TAL_F1 (ttggcgtcggcaaacagtgg)
3' 测序引物 TAL_R2 (ggcgacgaggtggtcgttg

文献引用方法
材料和方法部分: pCAG-T7-TALEN(Sangamo)-Destination was a gift from Pawel Pelczar (Addgene plasmid # 37184)
参考文献部分: Mouse genome engineering using designer nucleases. Hermann M, Cermak T, Voytas DF, Pelczar P. J Vis Exp. 2014 Apr 2;(86). doi: 10.3791/50930. 10.3791/50930 PubMed 24747757

载体质粒图谱和多克隆位点信息

pCAG-T7-TALEN(Sangamo)-Destination载体图谱

载体简介

载体序列

LOCUS       pCAG-T7-TALEN(Sangamo)-Destination	6441 bp 	DNA	SYN
DEFINITION  pCAG-T7-TALEN(Sangamo)-Destination
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="pCAG-T7-TALEN(Sangamo)-Destination"
                     /mol_type="other DNA"
     promoter        86..115
                     /label="lac_promoter"
     misc_feature    129..151
                     /label="M13_pUC_rev_primer"
     promoter        150..168
                     /label="M13_reverse_primer"
     misc_feature    264..551
                     /label="CAG_enhancer"
     CDS             complement(983..1714)
                     /label="ORF frame 3"
     CDS             complement(1024..1527)
                     /label="ORF frame 1"
     promoter        1864..1882
                     /label="T7_promoter"
     CDS             1893..2501
                     /label="ORF frame 3"
     misc_feature    1902..1922
                     /label="SV40 NLS"
     misc_feature    1908..1922
                     /label="NLS"
     misc_feature    complement(2357..2512)
                     /label="lacZ_a"
     misc_feature    2483..2505
                     /label="M13_pUC_fwd_primer"
     promoter        2498..2514
                     /label="M13_forward20_primer"
     promoter        2524..2542
                     /label="T7_promoter"
     promoter        complement(2589..2608)
                     /label="T3_promoter"
     promoter        complement(2625..2643)
                     /label="M13_reverse_primer"
     misc_feature    complement(2642..2664)
                     /label="M13_pUC_rev_primer"
     promoter        complement(2678..2707)
                     /label="lac_promoter"
     CDS             2722..3519
                     /label="ORF frame 1"
     misc_feature    complement(3538..3555)
                     /label="BGH_rev_primer"
     terminator      3541..3768
                     /label="bGH_PA_terminator"
     CDS             3742..4215
                     /label="ORF frame 1"
     promoter        complement(3840..3856)
                     /label="M13_forward20_primer"
     misc_feature    complement(3849..3871)
                     /label="M13_pUC_fwd_primer"
     misc_feature    3849..3997
                     /label="lacZ_a"
     rep_origin      4015..4321
                     /label="f1_origin"
     promoter        4514..4542
                     /label="AmpR_promoter"
     gene            4584..5444
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             4584..5444
                     /label="ORF frame 3"
     rep_origin      5599..6218
                     /label="pBR322_origin"
ORIGIN
    1 GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT
   61 AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG
  121 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAG GCGCGCCGGA
  181 TTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA
  241 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC
  301 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG
  361 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC
  421 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG
  481 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG
  541 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA
  601 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG
  661 CGATGGGGGC GGGGGGGGGG GGGGGGCGCG CGCCAGGCGG GGCGGGGCGG GGCGAGGGGC
  721 GGGGCGGGGC GAGGCGGAGA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT
  781 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG
  841 CGGGAGTCGC TGCGTTGCCT TCGCCCCGTG CCCCGCTCCG CGCCGCCTCG CGCCGCCCGC
  901 CCCGGCTCTG ACTGACCGCG TTACTCCCAC AGGTGAGCGG GCGGGACGGC CCTTCTCCTC
  961 CGGGCTGTAA TTAGCGCTTG GTTTAATGAC GGCTCGTTTC TTTTCTGTGG CTGCGTGAAA
 1021 GCCTTAAAGG GCTCCGGGAG GGCCCTTTGT GCGGGGGGGA GCGGCTCGGG GGGTGCGTGC
 1081 GTGTGTGTGT GCGTGGGGAG CGCCGCGTGC GGCCCGCGCT GCCCGGCGGC TGTGAGCGCT
 1141 GCGGGCGCGG CGCGGGGCTT TGTGCGCTCC GCGTGTGCGC GAGGGGAGCG CGGCCGGGGG
 1201 CGGTGCCCCG CGGTGCGGGG GGGCTGCGAG GGGAACAAAG GCTGCGTGCG GGGTGTGTGC
 1261 GTGGGGGGGT GAGCAGGGGG TGTGGGCGCG GCGGTCGGGC TGTAACCCCC CCCTGCACCC
 1321 CCCTCCCCGA GTTGCTGAGC ACGGCCCGGC TTCGGGTGCG GGGCTCCGTG CGGGGCGTGG
 1381 CGCGGGGCTC GCCGTGCCGG GCGGGGGGTG GCGGCAGGTG GGGGTGCCGG GCGGGGCGGG
 1441 GCCGCCTCGG GCCGGGGAGG GCTCGGGGGA GGGGCGCGGC GGCCCCGGAG CGCCGGCGGC
 1501 TGTCGAGGCG CGGCGAGCCG CAGCCATTGC CTTTTATGGT AATCGTGCGA GAGGGCGCAG
 1561 GGACTTCCTT TGTCCCAAAT CTGGCGGAGC CGAAATCTGG GAGGCGCCGC CGCACCCCCT
 1621 CTAGCGGGCG CGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG GGGAGGGCCT
 1681 TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCATCTCCAG CCTCGGGGCT GCCGCAGGGG
 1741 GACGGCTGCC TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC
 1801 GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GATCCTTAAT
 1861 TAATAATACG ACTCACTATA GGGGCCGCCA CCATGAGATC TCCTAAGAAA AAGAGGAAGG
 1921 TGCAGGTGGA TCTACGCACG CTCGGCTACA GTCAGCAGCA GCAAGAGAAG ATCAAACCGA
 1981 AGGTGCGTTC GACAGTGGCG CAGCACCACG AGGCACTGGT GGGCCATGGG TTTACACACG
 2041 CGCACATCGT TGCGCTCAGC CAACACCCGG CAGCGTTAGG GACCGTCGCT GTCACGTATC
 2101 AGCACATAAT CACGGCGTTG CCAGAGGCGA CACACGAAGA CATCGTTGGC GTCGGCAAAC
 2161 AGTGGTCCGG CGCACGCGCC CTGGAGGCCT TGCTCACGGA TGCGGGGGAG TTGAGAGGTC
 2221 CGCCGTTACA GTTGGACACA GGCCAACTTG TGAAGATTGC AAAACGTGGC GGCGTGACCG
 2281 CAATGGAGGC AGTGCATGCA TCGCGCAATG CACTGACGGG TGCCCCCCTG GAGACGGGCG
 2341 CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG
 2401 GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA
 2461 AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC
 2521 GCGTAATACG ACTCACTATA GGGCGAATTG GGTACCGGGC CCCCCCTCGA GGTCCTCCAG
 2581 CTTTTGTTCC CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGT CATAGCTGTT
 2641 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA
 2701 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT
 2761 GCCCGCTTTC CACCGGTCGT CTCCAACGAC CACCTCGTCG CCTTGGCCTG CCTCGGCGGA
 2821 CGTCCTGCCA TGGATGCAGT GAAAAAGGGA TTGCCGCACG CGCCGGAATT GATCAGAAGA
 2881 GTCAATCGCC GTATTGGCGA ACGCACGTCC CATCGCGTTg cggGATCcca gctagtgaaa
 2941 tctgaattgg aaGAGAAGAA ATCTGAACTT AGACATAAAT TGAAATATGT GCCACATGAA
 3001 TATATTGAAT TGATTGAAAT CGCAAGAAAT TCAACTCAGG ATAGAATCCT TGAAATGAAG
 3061 GTGATGGAGT TCTTTATGAA GGTTTATGGT TATCGTGGTA AACATTTGGG TGGATCAAGG
 3121 AAACCAGACG GAGCAATTTA TACTGTCGGA TCTCCTATTG ATTACGGTGT GATCGTTGAT
 3181 ACTAAGGCAT ATTCAGGAGG TTATAATCTT CCAATTGGTC AAGCAGATGA AATGCAAAGA
 3241 TATGTCGAAG AGAATCAAAC AAGAAACAAG CATATCAACC CTAATGAATG GTGGAAAGTC
 3301 TATCCATCTT CAGTAACAGA ATTTAAGTTC TTGTTTGTGA GTGGTCATTT CAAAGGAAAC
 3361 TACAAAGCTC AGCTTACAAG ATTGAATCAT ATCACTAATT GTAATGGAGC TGTTCTTAGT
 3421 GTAGAAGAGC TTTTGATTGG TGGAGAAATG ATTAAAGCTG GTACATTGAC ACTTGAGGAA
 3481 GTGAGAAGGA AATTTAATAA CGGTGAGATA AACTTTTAAT AGGAGCTCGC TGATCAGCCT
 3541 CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA
 3601 CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT
 3661 GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG
 3721 ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG
 3781 AAAGAACCAG CTGGGGCTCG AGATCCACTA GTTCTAGCCT CGAGGCTAGA GCGGCCGCCA
 3841 CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC
 3901 CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC
 3961 CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGACG CGCCCTGTAG CGGCGCATTA
 4021 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG
 4081 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA
 4141 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC
 4201 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT
 4261 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA
 4321 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC
 4381 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA
 4441 ACGCTTACAA TTTAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT
 4501 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC
 4561 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT
 4621 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG
 4681 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA
 4741 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC
 4801 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA
 4861 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG
 4921 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG
 4981 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA
 5041 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA
 5101 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA
 5161 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA
 5221 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT
 5281 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC
 5341 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA
 5401 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT
 5461 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA
 5521 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG
 5581 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA
 5641 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG
 5701 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG
 5761 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT
 5821 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA
 5881 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG
 5941 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC
 6001 GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA
 6061 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC
 6121 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT
 6181 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
 6241 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC
 6301 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG
 6361 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT
 6421 GGCCGATTCA TTAATGCAGC T
//

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