pACYC184

价格:800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: ATCC
载体名称: pACYC184
质粒类型: 原核表达系统载体
高拷贝/低拷贝: 低拷贝
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4245bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Chloramphenicol
筛选标记: Tetracycline
备注: --
产品目录号: 37033
稳定性: --
组成型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pACYC184载体图谱



pACYC184载体图谱2



载体简介

载体序列

LOCUS       pACYC184	4245 bp 	DNA	SYN
DEFINITION  pACYC184
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="pACYC184"
                     /mol_type="other DNA"
     gene            complement(4..219)
                     /label="CAT/CamR (659 - 444)"
                     /gene="CAT/CamR (659 - 444)"
     rep_origin      581..1493
                     /label="p15A_origin"
     gene            1581..2771
                     /label="tet"
                     /gene="tet"
     CDS             1581..2771
                     /label="ORF frame 3"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST*"
     misc_feature    complement(1933..1952)
                     /label="pBRrevBam_primer"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST*"
     CDS             complement(2118..2576)
                     /label="ORF frame 2"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
     gene            complement(3805..219)
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
     CDS             complement(3805..219)
                     /label="ORF frame 1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
ORIGIN
    1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
   61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
  121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
  181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
  241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
  301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
  361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
  421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
  481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
  541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
  601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
  661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
  721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
  781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
  841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
  901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
  961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
 1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
 1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
 1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
 1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
 1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
 1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
 1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
 1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
 1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
 1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
 1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
 1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
 1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
 1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
 1861 ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
 1921 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
 1981 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
 2041 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
 2101 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct
 2161 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
 2221 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg
 2281 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
 2341 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
 2401 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc
 2461 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
 2521 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
 2581 gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga
 2641 tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag
 2701 gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca
 2761 cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg
 2821 agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc
 2881 catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg
 2941 gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg
 3001 ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg
 3061 caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag
 3121 tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc
 3181 aaccggtgat accacgatac tatgactgag agtcaacgcc atgagcggcc tcatttctta
 3241 ttctgagtta caacagtccg caccgctgtc cggtagctcc ttccggtggg cgcggggcat
 3301 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
 3361 ggcagcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
 3421 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
 3481 acctacatct gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa
 3541 atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca
 3601 tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag
 3661 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg
 3721 aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg
 3781 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
 3841 taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat
 3901 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
 3961 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
 4021 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
 4081 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
 4141 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
 4201 aacactatcc catatcacca gctcaccgtc tttcattgcc atacg
//

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