pLZ269

LOCUS       pLZ269                7585 bp DNA     circular SYN 13-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..191
                     /label=182-WKG-596-F
     misc_feature    232
                     /label=CMV
     promoter        245..441
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     regulatory      467..476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    473..535
                     /label=Igk secretion signal
     regulatory      548..557
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             556
                     /codon_start=1
                     /label=5xGS Linker
                     /translation=""
     CDS             557..619
                     /codon_start=1
                     /label=ANR
                     /translation="GELDELVYLLDGPGYDPIHSD"
     CDS             620..694
                     /codon_start=1
                     /label=5xGS Linker
                     /translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
     CDS             695..757
                     /codon_start=1
                     /label=ANR
                     /translation="GELDELVYLLDGPGYDPIHSD"
     CDS             758..832
                     /codon_start=1
                     /label=5xGS Linker
                     /translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
     CDS             839..901
                     /codon_start=1
                     /label=ANR
                     /translation="GELDELVYLLDGPGYDPIHSD"
     CDS             902..976
                     /codon_start=1
                     /label=5xGS Linker
                     /translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
     CDS             977..1039
                     /codon_start=1
                     /label=ANR
                     /translation="GELDELVYLLDGPGYDPIHSD"
     CDS             1040..1114
                     /codon_start=1
                     /label=5xGS Linker
                     /translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
     misc_feature    1127..1870
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     primer_bind     complement(1194..1216)
                     /label=186-WKG-603-R
     misc_feature    1400..1402
                     /label=F93A
                     /note="F93A"
     misc_feature    1871..3141
                     /label=mFGFR 405-end
                     /note="mFGFR 405-end"
     misc_feature    2705..2707
                     /label=Y677
                     /note="Y677"
     polyA_signal    3182..3406
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3452..3880
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3466..3490)
                     /label=184-WKG-599-R
     promoter        3894..4223
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4290..5084
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5258..5379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5424..5444)
                     /label=M13-rev
     primer_bind     complement(5428..5444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(5450..5472)
                     /label=LacO
     protein_bind    5452..5468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5476..5506)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(5477..5506)
                     /label=lac
     protein_bind    5521..5542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5648..6588
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(6589..7449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7450..7554)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgatctgc
      241 gatctgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      301 ctaactccgc ccagttccgc ccattctccg ccccatcgct gactaatttt ttttatttat
      361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      421 ggaggcctag gcttttgcaa aaagctacga atcgcgctag tggccggcca ccatggagac
      481 cgatacctta ttactgtggg tgcttcttct gtgggttccc ggtagcaccg gagacggatc
      541 caagcttgcc accatggggg agctcgacga gttggtttat ctcttagacg gacccggata
      601 tgaccccatt cattcagatg gctccggaac gggcagcggg accggaagtg gcaccggaac
      661 cacttccgga accggtactg gcgggagtac cggcggtgag ctagacgaac tcgtgtacct
      721 gttggatggc cccggttacg acccaatcca cagcgatgga agtggaactg ggtctgggac
      781 tgggtccggc accgggacta cttcaggcac cggcactggg ggctccactg gccataccgg
      841 ggagctcgac gagttggttt atctcttaga cggacccgga tatgacccca ttcattcaga
      901 tggctccgga acgggcagcg ggaccggaag tggcaccgga accacttccg gaaccggtac
      961 tggcgggagt accggcggtg agctagacga actcgtgtac ctgttggatg gccccggtta
     1021 cgacccaatc cacagcgatg gaagtggaac tgggtctggg actgggtccg gcaccgggac
     1081 tacttcaggc accggcactg ggggctccac tggccatatg gaattcgcac cttcacccag
     1141 cctcccggac cccaagtttg agagcaaagc ggccctgctg gcatcccggg gctccgaaga
     1201 acttctgtgc ttcacccaac gcttggaaga cttggtgtgt ttctgggagg aagcggcgag
     1261 ctccgggatg gacttcaact acagcttctc ataccagctc gagggtgagt cacgaaagtc
     1321 atgtagcctg caccaggctc ccaccgtccg cggctccgtg cgtttctggt gttcactgcc
     1381 aacagcggac acatcgagtg ctgtgccgct ggagctgcag gtgacggagg cgtccggttc
     1441 tcctcgctat caccgcatca tccatatcaa tgaagtagtg ctcctggacg cccccgcggg
     1501 gctgctggcg cgccgggcag aagagggcag ccacgtggtg ctgcgctggc tgccacctcc
     1561 tggagcacct atgaccaccc acatccgata tgaagtggac gtgtcggcag gcaaccgggc
     1621 aggagggaca caaagggtgg aggtcctgga aggccgcact gagtgtgttc tgagcaacct
     1681 gcggggcggg acgcgctaca ccttcgctgt tcgagcgcgc atggccgagc cgagcttcag
     1741 cggattctgg agtgcctggt ctgagcccgc gtcactactg accgctagcg acctggaccc
     1801 tctcatcttg acgctgtctc tcattctggt cctcatctcg ttgttgctga cggttctggc
     1861 cctgctgtcc atgaagagcg gcaccaagaa gagcgacttc catagccaga tggctgtgca
     1921 caagctggcc aagagcatcc ctctgcgcag acaagtaaca gtgtcagctg actccagtgc
     1981 atccatgaac tctggggttc tcctggttcg gccctcacgg ctctcctcca gcgggacccc
     2041 catgctggct ggagtctccg aatatgagct ccctgaggac ccccgctggg agctgccacg
     2101 agacagactg gtcttaggca aaccacttgg cgagggctgc ttcgggcagg tggtgttggc
     2161 tgaggccatc gggctggata aggacaaacc caaccgtgtg accaaagtgg ccgtgaagat
     2221 gttgaagtcc gacgcaacgg agaaggacct gtcggatctg atctcggaga tggagatgat
     2281 gaaaatgatt gggaagcaca agaatatcat caaccttctg ggagcgtgca cacaggatgg
     2341 tcctctttat gtcattgtgg agtacgcctc caaaggcaat ctccgggagt atctacaggc
     2401 ccggaggcct cctgggctgg agtactgcta taaccccagc cacaaccccg aggaacagct
     2461 gtcttccaaa gatctggtat cctgtgccta tcaggtggct cggggcatgg agtatcttgc
     2521 ctctaagaag tgtatacacc gagacctggc tgctaggaac gtcctggtga ccgaggataa
     2581 cgtaatgaag atcgcagact ttggcttagc tcgagacatt catcatatcg actactacaa
     2641 gaaaaccacc aacggccggc tgcctgtgaa gtggatggcc cctgaggcgt tgtttgaccg
     2701 gatctacaca caccagagcg atgtgtggtc ttttggagtg ctcttgtggg agatcttcac
     2761 tctgggtggc tccccatacc ccggtgtgcc tgtggaggaa cttttcaagc tgctgaagga
     2821 gggtcatcga atggacaagc ccagtaactg taccaatgag ctgtacatga tgatgcggga
     2881 ctgctggcat gcagtgccct ctcagagacc tacgttcaag cagttggtgg aagacctgga
     2941 ccgcattgtg gccttgacct ccaaccagga gtatctggac ctgtccatac cgctggacca
     3001 gtactcaccc agctttcccg acacacggag ctccacctgc tcctcagggg aggactctgt
     3061 cttctctcat gagccgttac ctgaggagcc ctgtctgcct cgacacccca cccagcttgc
     3121 caacagtgga ctcaaacgcg ctagtctaga gggcccgttt aaacccgctg atcagcctcg
     3181 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3241 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3301 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3361 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3421 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     3481 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3541 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3601 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3661 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3721 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3781 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     3841 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     3901 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3961 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4021 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4141 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     4201 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     4261 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     4321 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4381 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4441 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     4501 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4561 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4621 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4681 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4741 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4801 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     4861 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4921 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     4981 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5041 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     5101 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     5161 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5221 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     5281 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5341 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     5401 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7561 tccccgaaaa gtgccacctg acgtc
//