| 质粒名称: | pHAGE2-TetOminiCMV-SV40LT |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 136616 |
| 存储实验室: | Hans Schöler |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE2 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6000 bp |
| 完整质粒大小: | 8857 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SV40 large T antigen,SV40LT,TAg |
| 物种来源: | Simian Vacuolating Virus 40 |
| 插入大小: | 2600 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHAGE2-TetOminiCMV-SV40LT was a gift from Hans Schöler (Addgene plasmid # 136616 ; http://n2t.net/addgene:136616 ; RRID:Addgene_136616) |
| 参考文献部分: | Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR. Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 10.1016/j.stem.2019.10.002. 10.1016/j.stem.2019.10.002 PubMed 31708402 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHAGE2-TetOminiCMV-SV40LT 8857 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Dox-inducible lentiviral vector expressing SV40 large T antigen for
immortalizing cells.
ACCESSION .
VERSION .
KEYWORDS pHAGE2-TetOminiCMV-SV40LT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8857)
AUTHORS Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR
TITLE Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental
Potential of iPSCs.
JOURNAL Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi:
10.1016/j.stem.2019.10.002.
PUBMED 31708402
REFERENCE 2 (bases 1 to 8857)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8857
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2251..2521
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
primer_bind 2589..2613
/label=LNCX
/note="Human CMV promoter, forward primer"
primer_bind 2684..2700
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2684..2700
/label=pBluescriptSK
/note="For pBluescript vector"
CDS join(2778..3023,3370..5250)
/codon_start=1
/product="SV40 (simian virus 40) large T antigen"
/label=large T antigen
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
CFKKPPTPPPEPET"
intron 3024..3369
/label=large T intron
/note="SV40 (simian virus 40) large T antigen intron"
misc_feature 5299..5887
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5352..5372)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5770..5781)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6035..6215
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6262..6280)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6348..6452
/gene="bla"
/label=AmpR promoter
CDS 6453..7313
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6671..6690)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7484..8072
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7973..7992
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8226..8243
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 8335..8354
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 8483..8504
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8519..8549
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8557..8573
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8562..8584
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8581..8597
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8581..8597
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 8647..8666
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc atgtacggtg ggaggcctat
2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgctctagaa ctagtggatc
2701 cgcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
2761 caaaaagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg
2821 accttctagg tcttgaaagg agtgcctggg ggaatattcc tctgatgaga aaggcatatt
2881 taaaaaaatg caaggagttt catcctgata aaggaggaga tgaagaaaaa atgaagaaaa
2941 tgaatactct gtacaagaaa atggaagatg gagtaaaata tgctcatcaa cctgactttg
3001 gaggcttctg ggatgcaact gaggtatttg cttcttcctt aaatcctggt gttgatgcaa
3061 tatactgcaa acaatggcct gagtgtgtaa agaaaatgtc tactaactgc atatgcttgc
3121 tgtgcttact gaggatgaag catgaaaata gaaaattata caggaaagat ccacttgtgt
3181 gggttgattg ttactgcttc gattgcttta gaatgtggtt tggacttgat ctttgtgaag
3241 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
3301 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
3361 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
3421 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
3481 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
3541 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
3601 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
3661 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
3721 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
3781 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
3841 ccattttctg ttattgagga aagtttgcca ggtgggttaa aggagcatga ttttaatcca
3901 gaagaagcag aggaaactaa acaagtgtcc tggaagcttg taacagagta tgcaatggaa
3961 acaaaatgtg atgatgtgtt gttattgctt gggatgtact tggaatttca atacagtttt
4021 gaaatgtgtt taaaatgtat taaaaaagaa cagcccagcc actataagta ccatgaaaag
4081 cattatgcaa atgctgctat atttgctgac agcaaaaacc aaaaaaccat atgccaacag
4141 gctgttgata ctgttttagc taaaaagcgg gttgatagcc tacaactaac tagagaacaa
4201 atgttaacaa acagatttaa tgatcttttg gataggatgg atataatgtt tggttctaca
4261 ggctctgctg acatagaaga atggatggct ggagttgctt ggctacactg tttgttgccc
4321 aaaatggatt cagtggtgta tgacttttta aaatgcatgg tgtacaacat tcctaaaaaa
4381 agatactggc tgtttaaagg accaattgat agtggtaaaa ctacattagc agctgctttg
4441 cttgaattat gtggggggaa agctttaaat gttaatttgc ccttggacag gctgaacttt
4501 gagctaggag tagctattga ccagttttta gtagtttttg aggatgtaaa gggcactgga
4561 ggggagtcca gagatttgcc ttcaggtcag ggaattaata acctggacaa tttaagggat
4621 tatttggatg gcagtgttaa ggtaaactta gaaaagaaac acctaaataa aagaactcaa
4681 atatttcccc ctggaatagt caccatgaat gagtacagtg tgcctaaaac actgcaggcc
4741 agatttgtaa aacaaataga ttttaggccc aaagattatt taaagcattg cctggaacgc
4801 agtgagtttt tgttagaaaa gagaataatt caaagtggca ttgctttgct tcttatgtta
4861 atttggtaca gacctgtggc tgagtttgct caaagtattc agagcagaat tgtggagtgg
4921 aaagagagat tggacaaaga gtttagtttg tcagtgtatc aaaaaatgaa gtttaatgtg
4981 gctatgggaa ttggagtttt agattggcta agaaacagtg atgatgatga tgaagacagc
5041 caggaaaatg ctgataaaaa tgaagatggt ggggagaaga acatggaaga ctcagggcat
5101 gaaacaggca ttgattcaca gtcccaaggc tcatttcagg cccctcagtc ctcacagtct
5161 gttcatgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
5221 cccacacctc cccctgaacc tgaaacataa aggatcctta agctagcgaa ttcgatatca
5281 agcttatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
5341 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
5401 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
5461 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
5521 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
5581 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
5641 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
5701 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
5761 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
5821 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
5881 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
5941 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatcacct
6001 gcaggacagg cgcgccctgc tttttgcttg tactgggtct ctctggttag accagatctg
6061 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
6121 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
6181 cagacccttt tagtcagtgt ggaaaatctc tagcacccgg gcgattaagg aaagggctag
6241 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
6301 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
6361 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
6421 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
6481 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
6541 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
6601 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
6661 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
6721 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
6781 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
6841 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
6901 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
6961 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
7021 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
7081 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
7141 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
7201 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
7261 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
7321 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
7381 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
7441 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
7501 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
7561 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
7621 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
7681 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
7741 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
7801 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
7861 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
7921 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
7981 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
8041 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
8101 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
8161 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
8221 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
8281 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
8341 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
8401 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
8461 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
8521 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
8581 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
8641 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
8701 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
8761 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
8821 attgtatttg ttaaatatgt actacaaact tagtagt
//
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