| 质粒名称: | pSL173 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000641 |
| 产品用途: | Mammalian VanR-specific SEAP expression vector (VanO5-PhCMVmin-SEAP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | VanO5-PhCMVmin |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pSL173
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL173载体质粒基本信息
pSL173质粒图谱载体图谱和pSL173载体序列质粒序列多克隆位点信息
pSL173质粒载体简介
pSL173质粒序列载体序列
LOCUS pSL173 6478 bp DNA circular SYN 14-JAN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6478)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6478
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 214..238
/label=olyl219
misc_feature 228
/label=E2F-1
misc_feature 234..245
/label=VanO
/label=nonstandard type: operator
misc_feature 248..259
/label=VanO
/label=nonstandard type: operator
misc_feature 262..273
/label=VanO
/label=nonstandard type: operator
misc_feature 276..287
/label=VanO
/label=nonstandard type: operator
misc_feature 290..301
/label=VanO
/label=nonstandard type: operator
primer_bind 302..323
/label=Oscl23-1
misc_feature 302..306
/label=E2F-1
misc_feature 307..455
/label=hcmvmin
primer_bind complement(440..455)
/label=oYW11
misc_feature 478..502
/label=New Feature
primer_bind complement(479..499)
/label=olyl220
primer_bind 503..529
/label=Oscl13-1
misc_feature 504..2024
/label=SEAP
primer_bind complement(2004..2032)
/label=Oscl23-2
primer_bind complement(2006..2024)
/label=Oscl13-2
polyA_signal 2075..2299
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2345..2773
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2787..3116
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3183..3977
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4151..4272
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4317..4337)
/label=M13-rev
primer_bind complement(4321..4337)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(4343..4365)
/label=LacO
protein_bind 4345..4361
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4369..4399)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(4370..4399)
/label=lac
protein_bind 4414..4435
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 4541..5481
/label=ColE1
/note="ColE1"
CDS complement(5482..6342)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6343..6447)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtattggat
241 ccaatgcatt ggatccaatg gattggatcc aatcgattgg atccaattga ttggatccaa
301 tctagctcga gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag
361 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
421 ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattcgagc tcgcccgggg
481 atcctctaga gtcagcttcc tgcatgctgc tgctgctgct gctgctgggc ctgaggctac
541 agctctccct gggcatcatc ccagttgagg aggagaaccc ggacttctgg aaccgcgagg
601 cagccgaggc cctgggtgcc gccaagaagc tgcagcctgc acagacagcc gccaagaacc
661 tcatcatctt cctgggcgat gggatggggg tgtctacggt gacagctgcc aggatcctaa
721 aagggcagaa gaaggacaaa ctggggcctg agatacccct ggccatggac cgcttcccat
781 atgtggctct gtccaagaca tacaatgtag acaaacatgt gccagacagt ggagccacag
841 ccacggccta cctgtgcggg gtcaagggca acttccagac cattggcttg agtgcagccg
901 cccgctttaa ccagtgcaac acgacacgcg gcaacgaggt catctccgtg atgaatcggg
961 ccaagaaagc agggaagtca gtgggagtgg taaccaccac acgagtgcag cacgcctcgc
1021 cagccggcac ctacgcccac acggtgaacc gcaactggta ctcggacgcc gacgtgcctg
1081 cctcggcccg ccaggagggg tgccaggaca tcgctacgca gctcatctcc aacatggaca
1141 ttgacgtgat cctaggtgga ggccgaaagt acatgtttcg catgggaacc ccagaccctg
1201 agtacccaga tgactacagc caaggtggga ccaggctgga cgggaagaat ctggtgcagg
1261 aatggctggc gaagcgccag ggtgcccggt atgtgtggaa ccgcactgag ctcatgcagg
1321 cttccctgga cccgtctgtg acccatctca tgggtctctt tgagcctgga gacatgaaat
1381 acgagatcca ccgagactcc acactggacc cctccctgat ggagatgaca gaggctgccc
1441 tgcgcctgct gagcaggaac ccccgcggct tcttcctctt cgtggagggt ggtcgcatcg
1501 accatggtca tcatgaaagc agggcttacc gggcactgac tgagacgatc atgttcgacg
1561 acgccattga gagggcgggc cagctcacca gcgaggagga cacgctgagc ctcgtcactg
1621 ccgaccactc ccacgtcttc tccttcggag gctaccccct gcgagggagc tccatcttcg
1681 ggctggcccc tggcaaggcc cgggacagga aggcctacac ggtcctccta tacggaaacg
1741 gtccaggcta tgtgctcaag gacggcgccc ggccggatgt taccgagagc gagagcggga
1801 gccccgagta tcggcagcag tcagcagtgc ccctggacga agagacccac gcaggcgagg
1861 acgtggcggt gttcgcgcgc ggcccgcagg cgcacctggt tcacggcgtg caggagcaga
1921 ccttcatagc gcacgtcatg gccttcgccg cctgcctgga gccctacacc gcctgcgacc
1981 tggcgccccc cgccggcacc accgacgccg cgcacccggg ttaagcggcc gctcgagtct
2041 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
2101 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
2161 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
2221 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
2281 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
2341 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
2401 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
2461 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
2521 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
2581 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
2641 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
2701 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
2761 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
2821 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
2881 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
2941 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
3001 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
3061 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
3121 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
3181 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
3241 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
3301 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
3361 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
3421 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
3481 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
3541 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
3601 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
3661 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
3721 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
3781 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
3841 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
3901 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
3961 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
4021 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
4081 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
4141 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
4201 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
4261 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
4321 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
4381 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
4441 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
4501 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
4561 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
4621 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
4681 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
4741 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
4801 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
4861 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
4921 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
4981 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
5041 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
5101 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
5161 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
5221 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
5281 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
5341 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
5401 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
5461 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
5521 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
5581 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
5641 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
5701 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
5761 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
5821 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
5881 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
5941 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
6001 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
6061 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
6121 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
6181 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
6241 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
6301 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
6361 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
6421 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
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