pSL890

LOCUS      pSL890             7163 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..191
                     /label=182-WKG-596-F
     misc_feature    232
                     /label=CMV
     promoter        245..441
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     regulatory      467..476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    473..535
                     /label=Igk secretion signal
     CDS             542..1129
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     misc_feature    1136..1879
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     misc_feature    1409..1411
                     /label=F93A
                     /note="F93A"
     misc_feature    1863..1879
                     /label=EpoR-D1_D2_TM
     misc_feature    1889..2717
                     /label=IL6ST
     misc_feature    1916..1942
                     /label=box1
     misc_feature    2240..2242
                     /label=Y759A
     polyA_signal    2760..2984
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3030..3458
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3044..3068)
                     /label=184-WKG-599-R
     primer_bind     3329..3348
                     /label=189-WKG-612-F
     promoter        3472..3801
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3868..4662
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3912..3939)
                     /label=188-WKG-610-R
     polyA_signal    4836..4957
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5002..5022)
                     /label=M13-rev
     primer_bind     complement(5006..5022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(5028..5050)
                     /label=LacO
     protein_bind    5030..5046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5054..5084)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(5055..5084)
                     /label=lac
     protein_bind    5099..5120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5226..6166
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(6167..7027)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7028..7132)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgatctgc
      241 gatctgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      301 ctaactccgc ccagttccgc ccattctccg ccccatcgct gactaatttt ttttatttat
      361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      421 ggaggcctag gcttttgcaa aaagctacga atcgcgctag tggccggcca ccatggagac
      481 cgatacctta ttactgtggg tgcttcttct gtgggttccc ggtagcaccg gagacggatc
      541 caagaagaag ggcagcgtgg tgatcgtggg cagaatcaac ctgagcggcg acaccgccta
      601 cagccagcag accagaggcc tggagggctg ccaggagacc agccagaccg gcagagacaa
      661 gaaccaggtg gagggcgagg tgcaggtggt gagcaccgcc acccagagct tcctggccac
      721 cagcatcaac ggcgtgctgt ggaccgtgta ccacggcgcc ggcaccagaa ccatcgccag
      781 ccccaagggc cccgtgaccc agatgtacac gaacgtggac aaggacttag tgggctggca
      841 ggccccccaa ggcagcagaa gcctgacccc ctgcacctgc ggcagcagcg acctgtacct
      901 ggtgaccaga cacgccgacg tgatccccgt gagaagaaga ggcgacagca gaggcagcct
      961 gctgagcccc agacccatca gctatctgaa aggctcaagc ggcggccccc tgttgtgtcc
     1021 cgccggccat gccgtgggca tcttcagagc cgccgtgagc accagaggcg tggccaaggc
     1081 cgtggacttc atccccgtgg agagcctgga gaccaccatg agaagccccg aattcgcacc
     1141 ttcacccagc ctcccggacc ccaagtttga gagcaaagcg gccctgctgg catcccgggg
     1201 ctccgaagaa cttctgtgct tcacccaacg cttggaagac ttggtgtgtt tctgggagga
     1261 agcggcgagc tccgggatgg acttcaacta cagcttctca taccagctcg agggtgagtc
     1321 acgaaagtca tgtagcctgc accaggctcc caccgtccgc ggctccgtgc gtttctggtg
     1381 ttcactgcca acagcggaca catcgagtgc tgtgccgctg gagctgcagg tgacggaggc
     1441 gtccggttct cctcgctatc accgcatcat ccatatcaat gaagtagtgc tcctggacgc
     1501 ccccgcgggg ctgctggcgc gccgggcaga agagggcagc cacgtggtgc tgcgctggct
     1561 gccacctcct ggagcaccta tgaccaccca catccgatat gaagtggacg tgtcggcagg
     1621 caaccgggca ggagggacac aaagggtgga ggtcctggaa ggccgcactg agtgtgttct
     1681 gagcaacctg cggggcggga cgcgctacac cttcgctgtt cgagcgcgca tggccgagcc
     1741 gagcttcagc ggattctgga gtgcctggtc tgagcccgcg tcactactga ccgctagcga
     1801 cctggaccct ctcatcttga cgctgtctct cattctggtc ctcatctcgt tgttgctgac
     1861 ggttctggcc ctgctgtccg cagcagcaaa taagcgagac ctaattaaaa aacacatctg
     1921 gcctaatgtt ccagatcctt caaagagtca tattgcccag tggtcacctc acactcctcc
     1981 aaggcacaat tttaattcaa aagatcaaat gtattcagat ggcaatttca ctgatgtaag
     2041 tgttgtggaa atagaagcaa atgacaaaaa gccttttcca gaagatctga aatcattgga
     2101 cctgttcaaa aaggaaaaaa ttaatactga aggacacagc agtggtattg gggggtcttc
     2161 atgcatgtca tcttctaggc caagcatttc tagcagtgat gaaaatgaat cttcacaaaa
     2221 cacttcgagc actgtccagg cttctaccgt ggtacacagt ggctacagac accaagttcc
     2281 gtcagtccaa gtcttctcaa gatccgagtc tacccagccc ttgttagatt cagaggagcg
     2341 gccagaagat ctacaattag tagatcatgt agatggcggt gatggtattt ttcccaggca
     2401 acagtacttc aaacagaact gcagtcagca tgaatccagt ccagatattt cacattttga
     2461 aaggtcaaag caagtttcat cagtcaatga ggaagatttt gttagactta aacagcagat
     2521 ttcagatcat atttcacaat cctgtggatc tgggcaaatg aaaatgtttc aggaagtttc
     2581 tgcagcagat gcttttggtc caggtactga gggacaagta gaaagatttg aaacagttgg
     2641 catggaggct gcgactgatg aaggcatgcc taaaagttac ttaccacaga ctgtacggca
     2701 aggcggctac atgcctcagt aatctagagg gcccgtttaa acccgctgat cagcctcgac
     2761 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     2821 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     2881 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     2941 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     3001 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     3061 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3121 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3181 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3241 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3301 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3361 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     3421 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     3481 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3541 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3601 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3661 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3721 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     3781 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     3841 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     3901 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     3961 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     4021 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     4081 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     4141 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     4201 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     4261 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     4321 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     4381 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     4441 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     4501 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     4561 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     4621 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     4681 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     4741 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     4801 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     4861 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4921 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     4981 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5041 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5101 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5161 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5221 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5281 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5821 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     5881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6061 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7141 cccgaaaagt gccacctgac gtc
//