| 质粒名称: | pHR_pGK_LaG17_synNotch_Gal4VP64 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 79127 |
| 存储实验室: | Wendell Lim |
|
载体骨架基本信息 |
|
| 载体名称: | pHR PGK |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LaG17_synNotch_Gal4DBDVP64 |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR_pGK_LaG17_synNotch_Gal4VP64 was a gift from Wendell Lim (Addgene plasmid # 79127 ; http://n2t.net/addgene:79127 ; RRID:Addgene_79127) |
| 参考文献部分: | Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim WA. Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. Epub 2016 Jan 28. 10.1016/j.cell.2016.01.012 PubMed 26830878 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR_pGK_LaG17_synNotch_Gal4VP64 11116 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral vector for constitutive expression of the LaG17 GFP
nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter).
ACCESSION .
VERSION .
KEYWORDS pHR_pGK_LaG17_synNotch_Gal4VP64
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11116)
AUTHORS Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim
WA
TITLE Engineering Customized Cell Sensing and Response Behaviors Using
Synthetic Notch Receptors.
JOURNAL Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012.
Epub 2016 Jan 28.
PUBMED 26830878
REFERENCE 2 (bases 1 to 11116)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11116
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2050..2167
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2227..2726
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(2262..2285)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 2659..2678
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
regulatory 2774..2783
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2843..2872
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 4712..4861
/codon_start=1
/product="tetrameric repeat of the minimal activation
domain of herpes simplex virus VP16 (Beerli et al., 1998)"
/label=VP64
/translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
DLDML"
misc_feature 4980..5568
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5033..5053)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5451..5462)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5571..5587)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5572..5588)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5678..5911
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(5936..5954)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(5937..5954)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(6244..6263)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(6477..6496)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6596..6618
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6656..6674)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6742..6846
/gene="bla"
/label=AmpR promoter
CDS 6847..7707
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7065..7084)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7878..8466
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8367..8386
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8620..8637
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(8707..8727)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8712..9041
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8892..9027
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8954..8973
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 10175..10240
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 10370..10390
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 10815..10949
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10852..10871)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10906..10925
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
2221 cgaattgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
2281 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
2341 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
2401 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
2461 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
2521 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
2581 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
2641 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
2701 cctcttcctc atctccgggc ctttcgaatt ctcacgcgtc aagtggagca aggcaggtgg
2761 acagtggatc gccgccacca tggcattgcc cgtgaccgcc ctgctgctgc cactggcctt
2821 gttgctccac gccgcgcggc cagaacagaa gctgatcagc gaggaggatc tgatggctga
2881 tgtgcaactc gtagagtccg gtggcggact cgtccaggcg ggaggctccc tgcgcctgag
2941 ttgcgctgcg tccggaagaa ctattagcat ggccgccatg agttggttca gacaggcgcc
3001 tggaaaggag agggagttcg tggcaggcat aagtcgctcc gcagggtcag ccgtccatgc
3061 cgactccgta aagggacgct ttaccatctc cagagataac acaaaaaaca cgctctatct
3121 ccaaatgaat tctttgaagg ccgaggacac agccgtgtac tactgcgccg ttaggaccag
3181 cggatttttt ggaagcattc ctaggacagg gacagccttt gattactggg gccagggaac
3241 gcaggttacc gtttccatcc tggactacag cttcacaggt ggcgctgggc gcgacattcc
3301 cccaccgcag attgaggagg cctgtgagct gcctgagtgc caggtggatg caggcaataa
3361 ggtctgcaac ctgcagtgta ataatcacgc atgtggctgg gatggtggcg actgctccct
3421 caacttcaat gacccctgga agaactgcac gcagtctcta cagtgctgga agtattttag
3481 cgacggccac tgtgacagcc agtgcaactc ggccggctgc ctctttgatg gcttcgactg
3541 ccagctcacc gagggacagt gcaaccccct gtatgaccag tactgcaagg accacttcag
3601 tgatggccac tgcgaccagg gctgtaacag tgccgaatgt gagtgggatg gcctagactg
3661 tgctgagcat gtacccgagc ggctggcagc cggcaccctg gtgctggtgg tgctgcttcc
3721 acccgaccag ctacggaaca actccttcca ctttctgcgg gagctcagcc acgtgctgca
3781 caccaacgtg gtcttcaagc gtgatgcgca aggccagcag atgatcttcc cgtactatgg
3841 ccacgaggaa gagctgcgca agcacccaat caagcgctct acagtgggtt gggccacctc
3901 ttcactgctt cctggtacca gtggtgggcg ccagcgcagg gagctggacc ccatggacat
3961 ccgtggctcc attgtctacc tggagatcga caaccggcaa tgtgtgcagt catcctcgca
4021 gtgcttccag agtgccaccg atgtggctgc cttcctaggt gctcttgcgt cacttggcag
4081 cctcaatatt ccttacaaga ttgaggccgt gaagagtgag ccggtggagc ctccgctgcc
4141 ctcgcagctg cacctcatgt acgtggcagc ggccgccttc gtgctcctgt tctttgtggg
4201 ctgtggggtg ctgctgtccc gcaagcgccg gcggatgaag ctgctgagca gcatcgagca
4261 ggcctgtgac atctgccggc tgaagaaact gaagtgcagc aaagaaaagc ccaagtgcgc
4321 caagtgcctg aagaacaact gggagtgccg gtacagcccc aagaccaaga gaagccccct
4381 gaccagagcc cacctgaccg aggtggaaag ccggctggaa agactggaac agctgtttct
4441 gctgatcttc ccacgcgagg acctggacat gatcctgaag atggacagcc tgcaggacat
4501 caaggccctg ctgaccggcc tgttcgtgca ggacaacgtg aacaaggacg ccgtgaccga
4561 cagactggcc agcgtggaaa ccgacatgcc cctgaccctg cggcagcaca gaatcagcgc
4621 caccagcagc agcgaggaaa gcagcaacaa gggccagcgg cagctgacag tgtctgctgc
4681 tgcaggcgga agcggaggct ctggcggatc tgatgccctg gacgacttcg acctggatat
4741 gctgggcagc gacgccctgg atgattttga tctggacatg ctgggatctg acgctctgga
4801 cgatttcgat ctcgacatgt tgggatcaga tgcactggat gactttgacc tggacatgct
4861 cggatcatga gatccttgac ttgcggccgc aactcccacc tgcaacatgc gtgactgact
4921 gaggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata
4981 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
5041 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
5101 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
5161 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
5221 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
5281 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
5341 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
5401 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
5461 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
5521 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
5581 gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta caaggcagct
5641 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
5701 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
5761 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
5821 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
5881 cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat
5941 agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt
6001 ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat
6061 catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg
6121 gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc
6181 agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
6241 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
6301 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
6361 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
6421 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca
6481 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
6541 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
6601 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
6661 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
6721 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
6781 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
6841 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
6901 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
6961 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
7021 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
7081 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
7141 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
7201 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
7261 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
7321 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
7381 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
7441 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
7501 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
7561 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
7621 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
7681 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
7741 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
7801 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
7861 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
7921 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
7981 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
8041 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
8101 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
8161 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
8221 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
8281 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
8341 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
8401 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
8461 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
8521 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
8581 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
8641 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
8701 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
8761 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
8821 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
8881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
8941 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
9001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag
9061 gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
9121 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
9181 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
9241 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
9301 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
9361 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
9421 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
9481 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
9541 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
9601 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
9661 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
9721 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
9781 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
9841 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
9901 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
9961 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
10021 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
10081 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
10141 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
10201 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
10261 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
10321 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
10381 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
10441 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
10501 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
10561 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
10621 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
10681 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
10741 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
10801 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
10861 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
10921 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
10981 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
11041 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
11101 ctacaaactt agtagt
//
