pCAMBIA1300-nLuc

LOCUS       pCambia1300-nLuc          11752 bp ds-DNA    circular SYN 28-01-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pCambia1300-nLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported from SnapGene 1.1.3
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11752
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        500..845
                     /note="CaMV 35S promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    854..921
                     /note="MCS"
                     /note="color: #ffff99"
     CDS             1035..2198
                     /codon_start=1
                     /note="nLuc"
                     /note="color: #ffff99"                    
     terminator      2205..2846
                     /note="E9 terminator"
                     /note="color: #ffffff"
     misc_feature    3073..3097
                     /note="RB T-DNA repeat"
                     /note="color: #ffe4c4"
     CDS             4397..5026
                     /codon_start=1
                     /note="pVS1 StaA"
                     /note="color: #993366"                     
     CDS             5460..6527
                     /codon_start=1
                     /note="pVS1 RepA"
                     /note="color: #993366"                     
     rep_origin      6593..6787
                     /note="pVS1 oriV"
                     /note="color: #ffff00"
     misc_feature    7131..7271
                     /note="bom"
                     /note="color: #a6acb3"
     rep_origin      complement(7457..8045)
                     /direction=LEFT
                     /note="ori"
                     /note="color: #ffff00"
     CDS             complement(8132..8926)
                     /codon_start=1
                     /note="KanR"
                     /note="color: #ccffcc"                     
     misc_feature    9351..9375
                     /note="LB T-DNA repeat"
                     /note="color: #ffe4c4"
     polyA_signal    9453..9627
                     /note="CaMV poly(A) signal"
                     /note="color: #a6acb3"
     CDS             complement(9667..10692)
                     /codon_start=1
                     /note="HygR"
                     /note="color: #ccffcc"                     
     promoter        complement(10760..11437)
                     /note="CaMV 35S promoter (enhanced)"
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      841 gaacacgggg gacgagctcg gtacccggga tcctctagag ggcccggcgc gccactagtg
      901 tcgaccagat ctcgtacgcg tcccggggcg gtggctcatc tggcggaggt gaagacgcca
      961 aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc
     1021 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
     1081 cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag
     1141 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
     1201 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
     1261 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg
     1321 tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa
     1381 tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
     1441 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt
     1501 ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc
     1561 ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc
     1621 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
     1681 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
     1741 tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg
     1801 cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg
     1861 atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg
     1921 aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga
     1981 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     2041 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     2101 atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc
     2161 cggaagcgac caacgccttg attgacaagg atggatgact gcagagcttt cgttcgtatc
     2221 atcggtttcg acaacgttcg tcaagttcaa tgcatcagtt tcattgcgca cacaccagaa
     2281 tcctactgag ttcgagtatt atggcattgg gaaacatgtt tttcttgtac catttgttgt
     2341 gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa atggaaatgg
     2401 atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta atattatttg
     2461 ttttttctct tatttgttgt gtgttgaatt tgaaaatata agagatatgc aaacattttg
     2521 ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata tgaggagtaa
     2581 aacacttgta gttgtaccat tatgcttatt cactaggcaa caaatatatt ttcagaccta
     2641 gaaaagctgc aaatgttact gaatacaagt atgtcctctt gtgttttaga catttatgaa
     2701 ctttccttta tgtaattttc cagaatcctt gtcagattct aatcattgct ttataattat
     2761 agttatactc atggatttgt agttgagtat gaaaatattt tttaatgcat tttatgactt
     2821 gccaattgat tgacaacatg catcaagctt ggcactggcc gtcgttttac aacgtcgtga
     2881 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     2941 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3001 tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa
     3061 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     3121 gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     3181 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     3241 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
     3301 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     3361 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     3421 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     3481 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     3541 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     3601 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     3661 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     3721 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     3781 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     3841 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     3901 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     3961 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     4021 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     4081 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     4141 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     4201 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     4261 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     4321 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     4381 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     4441 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     4501 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     4561 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     4621 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     4681 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     4741 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     4801 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     4861 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     4921 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     4981 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     5041 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     5101 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     5161 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     5221 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     5281 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     5341 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     5401 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg
     5461 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     5521 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     5581 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     5641 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     5701 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     5761 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     5821 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     5881 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     5941 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     6001 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     6061 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     6121 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     6181 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     6241 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     6301 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     6361 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     6421 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     6481 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     6541 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata
     6601 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     6661 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     6721 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     6781 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     6841 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     6901 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     6961 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     7021 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7081 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     7141 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     7201 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     7261 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7321 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7381 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7441 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7501 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7561 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7621 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7681 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7741 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7801 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7861 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     7921 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7981 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8041 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8101 gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat
     8161 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
     8221 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
     8281 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
     8341 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
     8401 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
     8461 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
     8521 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
     8581 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
     8641 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
     8701 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
     8761 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
     8821 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
     8881 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
     8941 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
     9001 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
     9061 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
     9121 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
     9181 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
     9241 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
     9301 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
     9361 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
     9421 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt
     9481 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
     9541 tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc cttatctggg
     9601 aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat ccggtcggca
     9661 tctactctat ttctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
     9721 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
     9781 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
     9841 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
     9901 cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
     9961 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
    10021 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
    10081 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc
    10141 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
    10201 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
    10261 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
    10321 cgcagcgatc gcatccatag cctccgcgac cggttgtaga acagcgggca gttcggtttc
    10381 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
    10441 gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
    10501 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
    10561 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
    10621 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
    10681 aggctttttc atatctcatt gccccccggg atctgcgaaa gctcgagaga gatagatttg
    10741 tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt ccttatatag
    10801 aggaaggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc
    10861 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
    10921 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
    10981 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
    11041 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
    11101 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
    11161 gagagtgtcg tgctccacca tgttatcaca tcaatccact tgctttgaag acgtggttgg
    11221 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
    11281 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
    11341 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
    11401 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
    11461 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg
    11521 ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
    11581 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    11641 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    11701 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cg
//