pSL889

LOCUS       pSL889              7274 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232
                     /label=CMV
     promoter        245..441
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     regulatory      467..476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    473..535
                     /label=Igk secretion signal
     CDS             541
                     /codon_start=1
                     /label=linker
                     /translation=""
     CDS             542..1240
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     misc_feature    1247..1990
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     misc_feature    1520..1522
                     /label=F93A
                     /note="F93A"
     misc_feature    1974..1990
                     /label=EpoR-D1_D2_TM
     CDS             2000..2828
                     /codon_start=1
                     /label=IL6ST
                     /translation="NKRDLIKKHIWPNVPDPSKSHIAQWSPHTPPRHNFNSKDQMYSDG
                     NFTDVSVVEIEANDKKPFPEDLKSLDLFKKEKINTEGHSSGIGGSSCMSSSRPSISSSD
                     ENESSQNTSSTVQASTVVHSGYRHQVPSVQVFSRSESTQPLLDSEERPEDLQLVDHVDG
                     GDGIFPRQQYFKQNCSQHESSPDISHFERSKQVSSVNEEDFVRLKQQISDHISQSCGSG
                     QMKMFQEVSAADAFGPGTEGQVERFETVGMEAATDEGMPKSYLPQTVRQGGYMP"
     misc_feature    2027..2053
                     /label=box1
     misc_feature    2351..2353
                     /label=Y759A
     polyA_signal    2871..3095
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3141..3569
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3155..3179)
                     /label=184-WKG-599-R
     primer_bind     3440..3459
                     /label=189-WKG-612-F
     promoter        3583..3912
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3979..4773
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4023..4050)
                     /label=188-WKG-610-R
     polyA_signal    4947..5068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5113..5133)
                     /label=M13-rev
     primer_bind     complement(5117..5133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(5139..5161)
                     /label=LacO
     protein_bind    5141..5157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5165..5195)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(5166..5195)
                     /label=lac
     protein_bind    5210..5231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5337..6277
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(6278..7138)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7139..7243)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgatctgc
      241 gatctgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      301 ctaactccgc ccagttccgc ccattctccg ccccatcgct gactaatttt ttttatttat
      361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      421 ggaggcctag gcttttgcaa aaagctacga atcgcgctag tggccggcca ccatggagac
      481 cgatacctta ttactgtggg tgcttcttct gtgggttccc ggtagcaccg gagacggatc
      541 cgacatcgag aagctgtgca agaaggcgga agaggaggcc aaggaggccc aggagaaggc
      601 cgacgagctg agacaacgac accccgattc gcaggcggcg gaagacgcgg aggatctcgc
      661 aaatctggct gtcgccgccg tgctgaccgc ttgtctgctg gcccaggagc atcccaacgc
      721 cgatatcgct aagctgtgca tcaaggccgc gagcgaagct gcggaggccg cgtcaaaagc
      781 cgccgagctg gcccaacggc acccagacag tcaagcagcc agagatgcta tcaaactcgc
      841 ctctcaagcc gcccgcgccg tgatcctggc catcatgctc gccgcagaga atcccaacgc
      901 tgatattgca aagctatgta ttaaggcggc cagcgaagcc gcagaggctg cctcgaaagc
      961 tgcggaactt gcacagcggc accccgactc ccaggccgcc cgagatgcaa ttaaactggc
     1021 cagtcaggcc gctgaggcgg tggagagagc catctggctg gccgccgaga accccaatgc
     1081 cgatatcgcc aaaaaatgca tcaaagcggc ctccgaagca gccgaggagg cctctaaagc
     1141 tgccgaagag gctcaaagac acccagacag ccagaaggca cgcgacgaga tcaaggaggc
     1201 ctctcagaag gccgaggagg tgaaggagag atgcaagagc gaattcgcac cttcacccag
     1261 cctcccggac cccaagtttg agagcaaagc ggccctgctg gcatcccggg gctccgaaga
     1321 acttctgtgc ttcacccaac gcttggaaga cttggtgtgt ttctgggagg aagcggcgag
     1381 ctccgggatg gacttcaact acagcttctc ataccagctc gagggtgagt cacgaaagtc
     1441 atgtagcctg caccaggctc ccaccgtccg cggctccgtg cgtttctggt gttcactgcc
     1501 aacagcggac acatcgagtg ctgtgccgct ggagctgcag gtgacggagg cgtccggttc
     1561 tcctcgctat caccgcatca tccatatcaa tgaagtagtg ctcctggacg cccccgcggg
     1621 gctgctggcg cgccgggcag aagagggcag ccacgtggtg ctgcgctggc tgccacctcc
     1681 tggagcacct atgaccaccc acatccgata tgaagtggac gtgtcggcag gcaaccgggc
     1741 aggagggaca caaagggtgg aggtcctgga aggccgcact gagtgtgttc tgagcaacct
     1801 gcggggcggg acgcgctaca ccttcgctgt tcgagcgcgc atggccgagc cgagcttcag
     1861 cggattctgg agtgcctggt ctgagcccgc gtcactactg accgctagcg acctggaccc
     1921 tctcatcttg acgctgtctc tcattctggt cctcatctcg ttgttgctga cggttctggc
     1981 cctgctgtcc gcagcagcaa ataagcgaga cctaattaaa aaacacatct ggcctaatgt
     2041 tccagatcct tcaaagagtc atattgccca gtggtcacct cacactcctc caaggcacaa
     2101 ttttaattca aaagatcaaa tgtattcaga tggcaatttc actgatgtaa gtgttgtgga
     2161 aatagaagca aatgacaaaa agccttttcc agaagatctg aaatcattgg acctgttcaa
     2221 aaaggaaaaa attaatactg aaggacacag cagtggtatt ggggggtctt catgcatgtc
     2281 atcttctagg ccaagcattt ctagcagtga tgaaaatgaa tcttcacaaa acacttcgag
     2341 cactgtccag gcttctaccg tggtacacag tggctacaga caccaagttc cgtcagtcca
     2401 agtcttctca agatccgagt ctacccagcc cttgttagat tcagaggagc ggccagaaga
     2461 tctacaatta gtagatcatg tagatggcgg tgatggtatt tttcccaggc aacagtactt
     2521 caaacagaac tgcagtcagc atgaatccag tccagatatt tcacattttg aaaggtcaaa
     2581 gcaagtttca tcagtcaatg aggaagattt tgttagactt aaacagcaga tttcagatca
     2641 tatttcacaa tcctgtggat ctgggcaaat gaaaatgttt caggaagttt ctgcagcaga
     2701 tgcttttggt ccaggtactg agggacaagt agaaagattt gaaacagttg gcatggaggc
     2761 tgcgactgat gaaggcatgc ctaaaagtta cttaccacag actgtacggc aaggcggcta
     2821 catgcctcag taatctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     2881 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     2941 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     3001 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     3061 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     3121 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3181 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3241 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3301 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3361 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3421 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3481 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3541 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     3601 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3661 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3721 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3781 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3841 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3901 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     3961 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     4021 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4081 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4141 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     4201 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     4261 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4321 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     4381 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     4441 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     4501 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     4561 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     4621 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     4681 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     4741 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     4801 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     4861 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     4921 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     4981 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5041 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     5101 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5161 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     5221 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5281 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5341 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5401 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5461 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5521 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5581 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5641 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5701 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5761 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5821 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5881 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5941 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6001 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6181 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6241 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6301 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6361 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6421 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6481 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6541 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6601 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6661 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6721 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6781 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6841 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6901 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6961 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7021 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7081 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7141 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7201 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7261 tgccacctga cgtc
//