pLZ286

LOCUS       pLZ286             7098 bp DNA     circular SYN 13-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    191..198
                     /label=CRE (RIP)
     misc_feature    206..213
                     /label=CRE (RIP)
     misc_feature    221..228
                     /label=CRE (RIP)
     misc_feature    236..243
                     /label=CRE (RIP)
     promoter        274..305
                     /label=P_min
     regulatory      340..349
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    346..408
                     /label=Igk secretion signal
     CDS             415..1002
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     misc_feature    1009..1752
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     misc_feature    1282..1284
                     /label=F93A
                     /note="F93A"
     misc_feature    1736..1752
                     /label=EpoR-D1_D2_TM
     CDS             1762..2590
                     /codon_start=1
                     /label=IL6ST
                     /translation="NKRDLIKKHIWPNVPDPSKSHIAQWSPHTPPRHNFNSKDQMYSDG
                     NFTDVSVVEIEANDKKPFPEDLKSLDLFKKEKINTEGHSSGIGGSSCMSSSRPSISSSD
                     ENESSQNTSSTVQASTVVHSGYRHQVPSVQVFSRSESTQPLLDSEERPEDLQLVDHVDG
                     GDGIFPRQQYFKQNCSQHESSPDISHFERSKQVSSVNEEDFVRLKQQISDHISQSCGSG
                     QMKMFQEVSAADAFGPGTEGQVERFETVGMEAATDEGMPKSYLPQTVRQGGYMP"
     misc_feature    1789..1815
                     /label=box1
     misc_feature    2113..2115
                     /label=Y759A
     polyA_signal    2695..2919
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2965..3393
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2979..3003)
                     /label=184-WKG-599-R
     promoter        3407..3736
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3803..4597
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3847..3874)
                     /label=188-WKG-610-R
     polyA_signal    4771..4892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4937..4957)
                     /label=M13-rev
     primer_bind     complement(4941..4957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(4963..4985)
                     /label=LacO
     protein_bind    4965..4981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4989..5019)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(4990..5019)
                     /label=lac
     protein_bind    5034..5055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5161..6101
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(6102..6962)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6963..7067)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattggagc tcttacgcgt
      181 gctagcagcc tgacgtccga gagcctgacg tccgagagcc tgacgtccga gagcctgacg
      241 tccgagatct ctcgaggtcg acagcggaga ctctagaggg tatataatgg aagctcgact
      301 tccagcttgg caatccggta ctgttggtaa agaaagcttg ccaccatgga gaccgatacc
      361 ttattactgt gggtgcttct tctgtgggtt cccggtagca ccggagacgg atccaagaag
      421 aagggcagcg tggtgatcgt gggcagaatc aacctgagcg gcgacaccgc ctacagccag
      481 cagaccagag gcctggaggg ctgccaggag accagccaga ccggcagaga caagaaccag
      541 gtggagggcg aggtgcaggt ggtgagcacc gccacccaga gcttcctggc caccagcatc
      601 aacggcgtgc tgtggaccgt gtaccacggc gccggcacca gaaccatcgc cagccccaag
      661 ggccccgtga cccagatgta cacgaacgtg gacaaggact tagtgggctg gcaggccccc
      721 caaggcagca gaagcctgac cccctgcacc tgcggcagca gcgacctgta cctggtgacc
      781 agacacgccg acgtgatccc cgtgagaaga agaggcgaca gcagaggcag cctgctgagc
      841 cccagaccca tcagctatct gaaaggctca agcggcggcc ccctgttgtg tcccgccggc
      901 catgccgtgg gcatcttcag agccgccgtg agcaccagag gcgtggccaa ggccgtggac
      961 ttcatccccg tggagagcct ggagaccacc atgagaagcc ccgaattcgc accttcaccc
     1021 agcctcccgg accccaagtt tgagagcaaa gcggccctgc tggcatcccg gggctccgaa
     1081 gaacttctgt gcttcaccca acgcttggaa gacttggtgt gtttctggga ggaagcggcg
     1141 agctccggga tggacttcaa ctacagcttc tcataccagc tcgagggtga gtcacgaaag
     1201 tcatgtagcc tgcaccaggc tcccaccgtc cgcggctccg tgcgtttctg gtgttcactg
     1261 ccaacagcgg acacatcgag tgctgtgccg ctggagctgc aggtgacgga ggcgtccggt
     1321 tctcctcgct atcaccgcat catccatatc aatgaagtag tgctcctgga cgcccccgcg
     1381 gggctgctgg cgcgccgggc agaagagggc agccacgtgg tgctgcgctg gctgccacct
     1441 cctggagcac ctatgaccac ccacatccga tatgaagtgg acgtgtcggc aggcaaccgg
     1501 gcaggaggga cacaaagggt ggaggtcctg gaaggccgca ctgagtgtgt tctgagcaac
     1561 ctgcggggcg ggacgcgcta caccttcgct gttcgagcgc gcatggccga gccgagcttc
     1621 agcggattct ggagtgcctg gtctgagccc gcgtcactac tgaccgctag cgacctggac
     1681 cctctcatct tgacgctgtc tctcattctg gtcctcatct cgttgttgct gacggttctg
     1741 gccctgctgt ccgcagcagc aaataagcga gacctaatta aaaaacacat ctggcctaat
     1801 gttccagatc cttcaaagag tcatattgcc cagtggtcac ctcacactcc tccaaggcac
     1861 aattttaatt caaaagatca aatgtattca gatggcaatt tcactgatgt aagtgttgtg
     1921 gaaatagaag caaatgacaa aaagcctttt ccagaagatc tgaaatcatt ggacctgttc
     1981 aaaaaggaaa aaattaatac tgaaggacac agcagtggta ttggggggtc ttcatgcatg
     2041 tcatcttcta ggccaagcat ttctagcagt gatgaaaatg aatcttcaca aaacacttcg
     2101 agcactgtcc aggcttctac cgtggtacac agtggctaca gacaccaagt tccgtcagtc
     2161 caagtcttct caagatccga gtctacccag cccttgttag attcagagga gcggccagaa
     2221 gatctacaat tagtagatca tgtagatggc ggtgatggta tttttcccag gcaacagtac
     2281 ttcaaacaga actgcagtca gcatgaatcc agtccagata tttcacattt tgaaaggtca
     2341 aagcaagttt catcagtcaa tgaggaagat tttgttagac ttaaacagca gatttcagat
     2401 catatttcac aatcctgtgg atctgggcaa atgaaaatgt ttcaggaagt ttctgcagca
     2461 gatgcttttg gtccaggtac tgagggacaa gtagaaagat ttgaaacagt tggcatggag
     2521 gctgcgactg atgaaggcat gcctaaaagt tacttaccac agactgtacg gcaaggcggc
     2581 tacatgcctc agtaaggatc cactagtcca gtgtggtgga attctgcaga tatccagcac
     2641 agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     2701 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     2761 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     2821 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     2881 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     2941 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3001 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3061 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3121 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3181 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3241 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3301 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3361 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     3421 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3481 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3541 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3601 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3661 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3721 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     3781 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     3841 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     3901 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     3961 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     4021 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     4081 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     4141 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     4201 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     4261 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4321 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     4381 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     4441 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     4501 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     4561 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     4621 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     4681 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     4741 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     4801 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4861 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     4921 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     4981 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5041 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5101 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5161 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5221 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5281 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5341 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5401 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5461 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5521 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5581 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5641 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5701 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5761 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     5821 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5881 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5941 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6001 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6061 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6121 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6181 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6241 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6301 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6361 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6421 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6481 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6541 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6601 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6661 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6721 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6781 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6841 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6901 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6961 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7021 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7081 aaagtgccac ctgacgtc
//