pLenti6/TR

价格:2200元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pLenti6/TR
质粒类型: Gateway系统;慢病毒表达载体;四环素调控载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: --
载体大小: 8355 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
筛选标记: Blasticidin
备注: pLenti6/TR是四环素调控系统的调控载体,对应的应答载体为pLenti4/TO/V5-Dest。
产品目录号: V480-20
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLenti6/TR载体图谱



pLenti6/TR载体图谱2



载体简介

载体序列

LOCUS       pLenti6/TR	8354 bp 	DNA	SYN
DEFINITION  pLenti6/TR
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="pLenti6/TR"
                     /mol_type="other DNA"
     misc_feature    230..410
                     /label="HIV-1_5_LTR"
     misc_feature    230..410
                     /label="truncHIV-1_3_LTR"
     misc_feature    521..565
                     /label="HIV-1_psi_pack"
     CDS             953..1801
                     /label="ORF frame 2"

     misc_feature    1075..1308
                     /label="RRE"

     promoter        1813..2317
                     /label="CMV_immearly_promoter"

     misc_feature    1820..2107
                     /label="CAG_enhancer"

     misc_feature    2274..2294
                     /label="CMV_fwd_primer"

     promoter        2275..2344
                     /label="CMV_promoter"

     misc_feature    2318..2337
                     /label="pCEP_fwd_primer"

     misc_feature    2320..2344
                     /label="LNCX_primer"

     misc_feature    2552..3124
                     /label="bGlob_int"

     misc_feature    3132..3151
                     /label="pCAG_F_primer"

     promoter        3179..3197
                     /label="T7_promoter"

     CDS             3208..3855
                     /label="ORF frame 1"

     misc_feature    3211..3822
                     /label="TetR"

     misc_feature    complement(3942..3962)
                     /label="pBABE_3_primer"

     misc_feature    complement(3948..4163)
                     /label="SV40_enhancer"

     promoter        3960..4228
                     /label="SV40_promoter"

     rep_origin      4127..4204
                     /label="SV40_origin"

     misc_feature    4189..4208
                     /label="SV40pro_F_primer"

     promoter        4322..4389
                     /label="EM7_promoter"

     gene            4390..4788
                     /label="blast"
                     /gene="blast"

     misc_feature    4858..4873
                     /label="cPPT"

     misc_feature    4858..4879
                     /label="U3PPT"
    
     misc_feature    4875..4927
                     /label="delta_U3"

     misc_feature    4928..5108
                     /label="truncHIV-1_3_LTR"
                
     misc_feature    4928..5108
                     /label="HIV-1_5_LTR"

     terminator      5183..5302
                     /label="SV40_PA_terminator"

     misc_feature    5271..5290
                     /label="EBV_rev_primer"

     rep_origin      5320..5418
                     /label="SV40_origin"

     misc_feature    5403..5422
                     /label="SV40pro_F_primer"

     promoter        complement(5497..5515)
                     /label="T7_promoter"

     promoter        complement(5525..5541)
                     /label="M13_forward20_primer"

     misc_feature    complement(5534..5556)
                     /label="M13_pUC_fwd_primer"

     misc_feature    5534..5682
                     /label="lacZ_a"

     rep_origin      5700..6006
                     /label="f1_origin"

     promoter        6199..6227
                     /label="AmpR_promoter"

     gene            6269..7129
                     /label="Ampicillin"
                     /gene="Ampicillin"

     CDS             6269..7129
                     /label="ORF frame 2"

     rep_origin      7284..7903
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY

     promoter        8212..8241
                     /label="lac_promoter"

     misc_feature    8255..8277
                     /label="M13_pUC_rev_primer"

     promoter        8276..8294
                     /label="M13_reverse_primer"

     promoter        8311..8330
                     /label="T3_promoter"

ORIGIN
    1 AATGTAGTCT TATGCAATAC TCTTGTAGTC TTGCAACATG GTAACGATGA GTTAGCAACA
   61 TGCCTTACAA GGAGAGAAAA AGCACCGTGC ATGCCGATTG GTGGAAGTAA GGTGGTACGA
  121 TCGTGCCTTA TTAGGAAGGC AACAGACGGG TCTGACATGG ATTGGACGAA CCACTGAATT
  181 GCCGCATTGC AGAGATATTG TATTTAAGTG CCTAGCTCGA TACATAAACG GGTCTCTCTG
  241 GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC
  301 TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG
  361 TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG
  421 AACAGGGACT TGAAAGCGAA AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT
  481 GCTGAAGCGC GCACGGCAAG AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG
  541 ACTAGCGGAG GCTAGAAGGA GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA
  601 ATTAGATCGC GATGGGAAAA AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA
  661 AAACATATAG TATGGGCAAG CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA
  721 GAAACATCAG AAGGCTGTAG ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA
  781 TCAGAAGAAC TTAGATCATT ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG
  841 ATAGAGATAA AAGACACCAA GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT
  901 AAGACCACCG CACAGCAAGC GGCCGCTGAT CTTCAGACCT GGAGGAGGAG ATATGAGGGA
  961 CAATTGGAGA AGTGAATTAT ATAAATATAA AGTAGTAAAA ATTGAACCAT TAGGAGTAGC
 1021 ACCCACCAAG GCAAAGAGAA GAGTGGTGCA GAGAGAAAAA AGAGCAGTGG GAATAGGAGC
 1081 TTTGTTCCTT GGGTTCTTGG GAGCAGCAGG AAGCACTATG GGCGCAGCGT CAATGACGCT
 1141 GACGGTACAG GCCAGACAAT TATTGTCTGG TATAGTGCAG CAGCAGAACA ATTTGCTGAG
 1201 GGCTATTGAG GCGCAACAGC ATCTGTTGCA ACTCACAGTC TGGGGCATCA AGCAGCTCCA
 1261 GGCAAGAATC CTGGCTGTGG AAAGATACCT AAAGGATCAA CAGCTCCTGG GGATTTGGGG
 1321 TTGCTCTGGA AAACTCATTT GCACCACTGC TGTGCCTTGG AATGCTAGTT GGAGTAATAA
 1381 ATCTCTGGAA CAGATTTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA
 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA
 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA
 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT
 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT
 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG
 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGATA
 1801 AGCTTGGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA
 1861 CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC
 1921 TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA
 1981 AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG
 2041 GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT
 2101 AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG
 2161 GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG
 2221 GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT
 2281 GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA
 2341 GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GACTCTAGAG
 2401 GATCCACTAG TCCAGTGTGG TGGAATTCTG CAGATAGCTT GGTACCCGGG GATCCTCTAG
 2461 GGCCTCTGAG CTATTCCAGA AGTAGTGAAG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
 2521 AAAGCTCCGG ATCGATCCTG AGAACTTCAG GGTGAGTTTG GGGACCCTTG ATTGTTCTTT
 2581 CTTTTTCGCT ATTGTAAAAT TCATGTTATA TGGAGGGGGC AAAGTTTTCA GGGTGTTGTT
 2641 TAGAATGGGA AGATGTCCCT TGTATCACCA TGGACCCTCA TGATAATTTT GTTTCTTTCA
 2701 CTTTCTACTC TGTTGACAAC CATTGTCTCC TCTTATTTTC TTTTCATTTT CTGTAACTTT
 2761 TTCGTTAAAC TTTAGCTTGC ATTTGTAACG AATTTTTAAA TTCACTTTTG TTTATTTGTC
 2821 AGATTGTAAG TACTTTCTCT AATCACTTTT TTTTCAAGGC AATCAGGGTA TATTATATTG
 2881 TACTTCAGCA CAGTTTTAGA GAACAATTGT TATAATTAAA TGATAAGGTA GAATATTTCT
 2941 GCATATAAAT TCTGGCTGGC GTGGAAATAT TCTTATTGGT AGAAACAACT ACATCCTGGT
 3001 CATCATCCTG CCTTTCTCTT TATGGTTACA ATGATATACA CTGTTTGAGA TGAGGATAAA
 3061 ATACTCTGAG TCCAAACCGG GCCCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT
 3121 ACAGCTCCTG GGCAACGTGC TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGTA
 3181 ATACGACTCA CTATAGGGCG AATTGATATG TCTAGATTAG ATAAAAGTAA AGTGATTAAC
 3241 AGCGCATTAG AGCTGCTTAA TGAGGTCGGA ATCGAAGGTT TAACAACCCG TAAACTCGCC
 3301 CAGAAGCTAG GTGTAGAGCA GCCTACATTG TATTGGCATG TAAAAAATAA GCGGGCTTTG
 3361 CTCGACGCCT TAGCCATTGA GATGTTAGAT AGGCACCATA CTCACTTTTG CCCTTTAGAA
 3421 GGGGAAAGCT GGCAAGATTT TTTACGTAAT AACGCTAAAA GTTTTAGATG TGCTTTACTA
 3481 AGTCATCGCG ATGGAGCAAA AGTACATTTA GGTACACGGC CTACAGAAAA ACAGTATGAA
 3541 ACTCTCGAAA ATCAATTAGC CTTTTTATGC CAACAAGGTT TTTCACTAGA GAATGCATTA
 3601 TATGCACTCA GCGCTGTGGG GCATTTTACT TTAGGTTGCG TATTGGAAGA TCAAGAGCAT
 3661 CAAGTCGCTA AAGAAGAAAG GGAAACACCT ACTACTGATA GTATGCCGCC ATTATTACGA
 3721 CAAGCTATCG AATTATTTGA TCACCAAGGT GCAGAGCCAG CCTTCTTATT CGGCCTTGAA
 3781 TTGATCATAT GCGGATTAGA AAAACAACTT AAATGTGAAA GTGGGTCCGC GTACAGCGGA
 3841 TCCCGGGAAT TCTAGAGGGC CCGCGGTTCG AACAAAAACT CATCTCAGAA GAGGATCTGA
 3901 ATATGCATAC CGGTTAGTAA TGAGTTTGGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG
 3961 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA
 4021 GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT
 4081 GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC
 4141 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA
 4201 GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG
 4261 CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA
 4321 CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG
 4381 AACTAAACCA TGGCCAAGCC TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG
 4441 GCTACAATCA ACAGCATCCC CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT
 4501 AGCGACGGCC GCATCTTCAC TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA
 4561 GAACTCGTGG TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC
 4621 GCGATCGGAA ATGAGAACAG GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT
 4681 CTCGATCTGC ATCCTGGGAT CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA
 4741 GTTGGGATTC GTGAATTGCT GCCCTCTGGT TATGTGTGGG AGGGCTAAGC ACAATTCGAG
 4801 CTCGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA
 4861 AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATC TGCTTTTTGC
 4921 TTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG
 4981 GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG
 5041 TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT
 5101 CTCTAGCAGT AGTAGTTCAT GTCATCTTAT TATTCAGTAT TTATAACTTG CAAAGAAATG
 5161 AATATCAGAG AGTGAGAGGA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
 5221 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
 5281 CAAACTCATC AATGTATCTT ATCATGTCTG GCTCTAGCTA TCCCGCCCCT AACTCCGCCC
 5341 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT
 5401 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA
 5461 GGCTTTTTTG GAGGCCTAGG GACGTACCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC
 5521 GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA
 5581 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG
 5641 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGCG
 5701 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC
 5761 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC
 5821 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG
 5881 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG
 5941 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG
 6001 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT
 6061 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA
 6121 TATTAACGCT TACAATTTAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG
 6181 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT
 6241 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT
 6301 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT
 6361 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG
 6421 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA
 6481 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG
 6541 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT
 6601 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC
 6661 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA
 6721 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT
 6781 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT
 6841 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC
 6901 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA
 6961 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG
 7021 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG
 7081 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA
 7141 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA
 7201 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA
 7261 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
 7321 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
 7381 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
 7441 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
 7501 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
 7561 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
 7621 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
 7681 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
 7741 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
 7801 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
 7861 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
 7921 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
 7981 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
 8041 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC
 8101 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG
 8161 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT
 8221 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA
 8281 CAGCTATGAC CATGATTACG CCAAGCGCGC AATTAACCCT CACTAAAGGG AACAAAAGCT
 8341 GGAGCTGCAA GCTT
//

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