| 质粒名称: | pLYL67 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000594 |
| 产品用途: | Mammalian cAMP-responsive 4xANR-NSP3 expression vector (PCRE-(ANR)4-NSP3-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | pCRE |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | (ANR)4-NSP3 |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLYL67
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLYL67载体质粒基本信息
pLYL67质粒图谱载体图谱和pLYL67载体序列质粒序列多克隆位点信息
pLYL67质粒载体简介
pLYL67质粒序列载体序列
LOCUS pLYL67 4956 bp DNA circular SYN 13-JAN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4956)
AUTHORS iGEM CPU-CHINA 2020
TITLE Direct Submission
JOURNAL Exported Jul 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4956
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(169..237)
/label=LacZ alpha
primer_bind 281..297
/label=oMH18
misc_feature 298..451
/label=Transcription blocker
/label=Transcription\blocker
misc_feature 485..571
/label=CRE reg_Regulatory_Seq
misc_feature 496..503
/label=CRE
misc_feature 532..539
/label=CRE*
misc_feature 554..561
/label=CRE
promoter 578..724
/label=P_TAL
primer_bind 681..708
/label=180-WKG-592-F
CDS 741
/codon_start=1
/label=5xGS Linker
/translation=""
CDS 742..804
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 805..879
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 880..942
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 943..1017
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1024..1086
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1087..1161
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1162..1224
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1225..1299
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
misc_feature 1306..2247
/label=NSP3
misc_feature 1306..1329
/label=NSP3
primer_bind complement(1317..1336)
/label=179-WKG-592-R
primer_bind complement(2296..2305)
/label=oMH18
terminator 2343..2570
/label=bGH PA
rep_origin complement(2899..3581)
/direction=LEFT
/label=ColE1 origin
misc_feature complement(3679..4536)
/label=AmpR
/label=nonstandard type: resistance
promoter complement(4537..4641)
/label=AmpR promoter
rep_origin join(4668..4956,1..167)
/label=M13 origin
ORIGIN
1 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
61 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
121 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc attcgccatt
181 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc
241 caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat
301 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
361 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
421 agtgcaagtg caggtgccag aacatttctc tatcgatagg taccgagctc ttacgcgtgc
481 tagcgcacca gacagtgacg tcagctgcca gatcccatgg ccgtcatact gtgacgtctt
541 tcagacaccc cattgacgtc aatgggagaa cagatctgcc gccccgactg catctgcgtg
601 ttcgaattcg ccaatgacaa gacgctgggc ggggtttgtg tcatcataga actaaagaca
661 tgcaaatata tttcttccgg ggacaccgcc agcaaacgcg agcaacgggc cacggggatg
721 aagcagaagc ttgccaccat gggggagctc gacgagttgg tttatctctt agacggaccc
781 ggatatgacc ccattcattc agatggctcc ggaacgggca gcgggaccgg aagtggcacc
841 ggaaccactt ccggaaccgg tactggcggg agtaccggcg gtgagctaga cgaactcgtg
901 tacctgttgg atggccccgg ttacgaccca atccacagcg atggaagtgg aactgggtct
961 gggactgggt ccggcaccgg gactacttca ggcaccggca ctgggggctc cactggccat
1021 accggggagc tcgacgagtt ggtttatctc ttagacggac ccggatatga ccccattcat
1081 tcagatggct ccggaacggg cagcgggacc ggaagtggca ccggaaccac ttccggaacc
1141 ggtactggcg ggagtaccgg cggtgagcta gacgaactcg tgtacctgtt ggatggcccc
1201 ggttacgacc caatccacag cgatggaagt ggaactgggt ctgggactgg gtccggcacc
1261 gggactactt caggcaccgg cactgggggc tccactggcc atatgatgct caagatggag
1321 tctacacagc agatggctag ttcgattatt aacacttctt ttgaagccgc agtcgttgct
1381 gccacttcaa ctcttgaact tatgggcatt caatatgatt ataatgaagt ttatactaga
1441 gttaagagca aatttgatta cgtaatggat gattcaggtg ttaaaaacaa tctgttgggt
1501 aaagcggcaa ctattgatca ggcactgaat ggaaaatttg gttcagcagc aagaaataga
1561 aattggatgg ctgatactag aactacagct agactagatg aagatgtgaa caaacttaga
1621 atgatgctat catctaaagg aattgatcaa aaaatgagag tacttaatgc atgtttcaat
1681 gtgaagcgag ttccaggtaa atcatcatca ataattaaat gtactagatt aatgagagat
1741 aagattgaac gtggtgaagt cgaagtggat gattcgtttg ttgaagaaaa aatggaagtt
1801 gacacaattg attggaaatc tagatatgaa caattggaaa aacgatttga atcgctgaaa
1861 caaagggtga atgaaaaata tacaagttgg gtgcaaaaag caaaaaaggt aaatgaaaat
1921 atgtactctc tccaaaacgt tatatcacaa cagcagagcc agattgctga tttacaaaat
1981 tactgtaata aattggaagt tgatctacaa aataaaatta gttcacttgt gtcttcagtc
2041 gaatggtacc tgaaatcaat ggaattgcct gatgaaatta agacagatat tgaacaacaa
2101 ctaaattcca ttgatgtaat caatccaatt aatgctatag atgattttga atcactgatc
2161 agaaatatta tactcgacta tgacagaata tttttaatgt ttaaaggatt aatgaggcaa
2221 tgcaactatg aatatacgta tgaataggat ccactagtcc agtgtggtgg aattctgcag
2281 atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc
2341 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
2401 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
2461 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
2521 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
2581 ggaaagaacc agctggggct ctagggtcga ccgatgccct tgagagcctt caacccagtc
2641 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
2701 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc
2761 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
2821 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
2881 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
2941 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
3001 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
3061 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
3121 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
3181 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
3241 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
3301 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
3361 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
3421 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
3481 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
3541 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
3601 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
3661 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
3721 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
3781 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
3841 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
3901 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
3961 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
4021 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
4081 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
4141 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
4201 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
4261 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
4321 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
4381 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
4441 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
4501 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
4561 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
4621 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag
4681 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
4741 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
4801 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
4861 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
4921 gacggttttt cgccctttga cgttggagtc cacgtt
//
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