| 质粒名称: | pDESTsplice |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 32484 |
| 存储实验室: | Stefan Stamm |
|
载体骨架基本信息 |
|
| 载体名称: | pET01 |
| 载体出品公司: | Mo Bi Tec |
| 空载体大小: | 5730bp |
| 完整质粒大小: | 5730bp |
| 载体抗性: | Chloramphenicol and Ampicillin, 25 & 100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | ccdB Survival |
| 拷贝数: | 高拷贝 |
|
文献引用方法 |
|
| 材料和方法部分: | pDESTsplice was a gift from Stefan Stamm (Addgene plasmid # 32484 ; http://n2t.net/addgene:32484 ; RRID:Addgene_32484) |
| 参考文献部分: | Rapid generation of splicing reporters with pSpliceExpress. Kishore S, Khanna A, Stamm S. Gene. 2008 Dec 31;427(1-2):104-10. Epub 2008 Oct 1. 10.1016/j.gene.2008.09.021 PubMed 18930792 |
买家导航
pDESTsplice载体质粒基本信息
pDESTsplice质粒图谱载体图谱和pDESTsplice载体序列质粒序列多克隆位点信息
pDESTsplice质粒载体简介
pDESTsplice质粒序列载体序列
LOCUS pDESTsplice 5730 bp DNA circular SYN 15-DEC-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5730)
AUTHORS Kishore S, Khanna A, Stamm S
TITLE Rapid generation of splicing reporters with pSpliceExpress.
JOURNAL Gene. 2008 Dec 31;427(1-2):104-10. Epub 2008 Oct 1.
PUBMED 18930792
REFERENCE 2 (bases 1 to 5730)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene.";
date: "2008-12-31"; volume: "427(1-2)"; pages: "104-10. Epub 2008
Oct 1"
COMMENT Sequence Label: pDESTsplice
FEATURES Location/Qualifiers
source 1..5730
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 1049..1065
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 1050..1066
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1093..1217
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter join(1242..1247,1248..1265,1266..1272)
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 1326..2006
/codon_start=1
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
DPAY"
primer_bind complement(1392..1411)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 2326..2631
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 2510..2529
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(2672..2796)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(2806..2822)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2806..2822)
/label=pBluescriptSK
/note="For pBluescript vector"
primer_bind complement(3450..3468)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 3536..3640
/gene="bla"
/label=AmpR promoter
CDS join(3641..3709,3710..4501)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3859..3878)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
primer_bind 5051..5070
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tcaggatata gtagtttcgc ttttgcatag ggagggggaa atgtagtctt atgcaatact
61 cttgtagtct tgcaacatgc ttatgtaacg atgagttagc aacatgcctt ataaggaaag
121 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt atgatcgtgg tatgatcgtg
181 gtatgatcgt gccttattag gaaggcaaca gacgggtcta acacggattg gacgaaccac
241 tgaattccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgccat
301 ttgaccattc accacattgg tgtgcacctg ggttgatggc cggaccgttg attccctgac
361 gactacgagc acatgcatga agcagaaggc ttcatttggt gacaagcttc tgcatgctgc
421 tgctgctgct gctgctgggc ctgaggctac agctctccct gggcatcatc ccagttgagg
481 aggagaaccc ggacttctgg aaccgcgagg cagccgaggc cctgggtgcc gccaagaagc
541 tgcagcctgc acagacagcc gccaagaacc tcatcatctt cctgggcgat gggatggggg
601 tgtctacggt gacagctgcc aggatcgatc cgcttcctgc ccctgctggc cctgctcatc
661 ctctgggagc cccgccctgc ccaggctttt gtcaaacagc acctttgtgg ttctcacttg
721 gtggaagctc tctacctggt gtgtggggag cgtggattct tctacacacc catgtcccgc
781 cgcgaagtgg aggacccaca aggtaagctc tgctcctgaa ttaattccta tcccaagtgc
841 taactaccct gtttgtcttt cacccttgag accttgtaaa ttgtgcccta ggtgtggagg
901 gtctcaggct aaccagtggg gggcacattt ctgtgggcag ctagacatat gtaaacatgg
961 tagctgccaa gaaggagtga gaatccttcc ttaagtctcc taggtggtga cgggtggcta
1021 ggccccagga taggtaccgg gccccccctc gaggtcgacg gtatcgataa gctaattcct
1081 gcagcccatc aaacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat
1141 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
1201 catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg
1261 gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag
1321 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
1381 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
1441 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
1501 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
1561 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
1621 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
1681 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
1741 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
1801 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
1861 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
1921 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg caggcggggc
1981 gtaatctaga ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
2041 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat
2101 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
2161 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
2221 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
2281 ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt
2341 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
2401 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
2461 gtcccccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
2521 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
2581 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
2641 tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca
2701 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
2761 ttacgtttct cgttcagctt tcttgtacaa agtggttcga tgggggatcc actagttcta
2821 gagcggccgc caccgcggtg gagctcggta cccatttggg gaccccatag agcaccgcac
2881 cgaccgaggg atggtaacag gatgtgtagg ttttggaggc ccatatgtcc attcatgacc
2941 agtgacttgt ctcacagcca tgcaaccctt gcctcctgtg ctgacttagc aggggataaa
3001 gtgagagaaa gcctgggcta atcggggggt cgctcggctc ctcctaactg gattgtccta
3061 tgtgtctttg cttctgtgct gctgatgctc tgccctgtgc tgacatgacc tccctggcag
3121 tggcacaact ggagctgggt ggaggcccgt gaccttcaga ccttggcact ggaggtggcc
3181 cggcagaagc gcggcatcgt ggatcagtgc tgcaccagca tctgctctct ctaccaactg
3241 gagaactact gcaactaggc ccaccactac cctgtccacc cctctgcaat gaataaaacc
3301 tttgaaagag cactacaagt tgtgtgtaca tgcgtgcgtg tgcatatgtg gtgcgggggg
3361 aacatgagtg gggtcggctg gagtggtcgc ggcttaatct atctggcgat gataagctgt
3421 caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
3481 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
3541 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
3601 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
3661 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
3721 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
3781 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
3841 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
3901 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
3961 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
4021 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
4081 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
4141 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
4201 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
4261 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
4321 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
4381 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
4441 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
4501 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
4561 taaaaggatc taggtgaaga tccgtaatct gctgcttgca aacaaaaaaa ccaccgctac
4621 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
4681 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
4741 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
4801 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
4861 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
4921 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
4981 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
5041 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
5101 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
5161 acgtcgggat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc
5221 aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag
5281 tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca
5341 tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc
5401 caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt
5461 gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc
5521 atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag
5581 aagaatcata atggggaagg ccatccagcc tcgcgtcgag ctttttgcaa aagcctaggc
5641 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
5701 ctgcataaat aaaaaaaatt agtcagccat
//
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