| 载体名称: | pcDNA3.1(+)-puro , pcDNA3.1-puro |
|---|---|
| 载体又名: | pcDNA3.1(+)-puro载体，pcDNA3.1(+)-puro质粒，pcDNA3.1(+)-puro载体质粒 |
| 英文名称: | pcDNA3.1(+)-puro, pcDNA3.1(+)-puro Vector, pcDNA3.1(+)-puro Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 5.2 kb |
| 5’测序引物: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
| 3’测序引物: | BGH-R: TAGAAGGCACAGTCGAGG |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 常规细胞系（293、CHO等） |
| 产品目录号: | 211279 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
pcDNA3.1(+)-puro
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
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pcDNA3.1(+)-puro载体pcDNA3.1-puro质粒基本信息
pcDNA3.1(+)-puro质粒图谱pcDNA3.1-puro载体图谱和pcDNA3.1(+)-puro载体序列pcDNA3.1-puro质粒序列多克隆位点信息
-puro-Map.png)
pcDNA3.1(+)-puro质粒pcDNA3.1-puro载体简介
pcDNA3.1(+)-puro质粒序列pcDNA3.1-puro载体序列
Snapgene载体序列下载: pcDNA3.1-puro序列 .gb格式载体序列下载:pcDNA3.1-puro载体序列
LOCUS pcDNA3.1(+)-puro 5164 bp DNA circular SYN 20-JUN-2026 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5164) AUTHORS Biofeng TITLE Direct Submission JOURNAL Exported Jun 20, 2026 from SnapGene 8.1.0 https://www.biofeng.com COMMENT Sequence Label: pcDNA3.1(+)-puro FEATURES Location/Qualifiers source 1..5164 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" /note="color: #ffffff; direction: RIGHT" polyA_signal 1028..1252 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" /note="color: #a6acb3" rep_origin 1298..1726 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /note="color: #ffff00" promoter 1740..2069 /label=SV40 promoter /note="SV40 enhancer and early promoter" /note="color: #ffffff; direction: RIGHT" rep_origin 1920..2055 /label=SV40 ori /note="SV40 origin of replication" /note="color: #ffff00" CDS 2081..2680 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" polyA_signal 2840..2961 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" primer_bind complement(3010..3026) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" /note="color: #a020f0; direction: LEFT" protein_bind 3034..3050 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /note="color: #31849b" promoter complement(join(3058..3064,3065..3082,3083..3088)) /label=lac promoter /note="promoter for the E. coli lac operon" /note="This reverse directional feature has 3 segments: 1: 3058 .. 3064 / #ffffff / -10 2: 3065 .. 3082 / #ffffff 3: 3083 .. 3088 / #ffffff / -35" protein_bind 3103..3124 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /note="color: #31849b" rep_origin complement(3412..3997) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(join(4168..4959,4960..5028)) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 4168 .. 4959 / #ccffcc 2: 4960 .. 5028 / #ccffcc / signal sequence Cleavage site after base 4959" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5029..5133) /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: LEFT" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 961 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca 1021 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 1081 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 1141 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 1201 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 1261 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 1321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 1381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 1441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 1501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 1561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 1621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 1681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 1741 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 1801 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 1861 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 1921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 1981 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 2041 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg atgaccgagt acaagcccac 2101 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt 2161 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac 2221 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 2281 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 2341 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 2401 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 2461 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 2521 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 2581 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 2641 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ttcgaaatga ccgaccaagc 2701 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg 2761 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 2821 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 2881 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 2941 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 3001 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 3061 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 3121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 3181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 3241 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 3301 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 3361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 3421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 3481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 3541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 3601 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 3661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 3721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 3781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 3841 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 3901 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg 3961 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 4021 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 4081 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 4141 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 4201 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 4261 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 4321 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 4381 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 4441 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 4501 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 4561 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 4621 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 4681 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 4741 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 4801 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 4861 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 4921 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 4981 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 5041 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 5101 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 5161 cgtc //
pcDNA3.1(+)-puro载体图谱pcDNA3.1-puro质粒图谱pdf版和pcDNA3.1(+)-puro载体序列pcDNA3.1-puro质粒序列等相关资料下载
pcDNA3.1(+)-puro质粒pcDNA3.1-puro载体应用举例
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