| 质粒名称: | pSL1032 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000658 |
| 产品用途: | AAV2/8-vector for PhCMV-driven constitutive expression of 3xGNCR1-NSP3 (ITR-PhCMV-(GNCR1)3-NSP3-pA-ITR). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: |
pCMV
|
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | (GNCR1)3-NSP3 |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pSL1032
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1032载体质粒基本信息
pSL1032质粒图谱载体图谱和pSL1032载体序列质粒序列多克隆位点信息
pSL1032质粒载体简介
pSL1032质粒序列载体序列
LOCUS pSL1032 7028 bp DNA circular SYN 08-OCT-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 7028)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Jul 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7028
/mol_type="other DNA"
/organism="recombinant plasmid"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
primer_bind 115..136
/label=oSCL1014
enhancer 154..533
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 534..737
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 688..708
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 748..787
/label=oSCL1944
misc_feature 763..767
/label=NheI
primer_bind 768..787
/label=T7
primer_bind 785..841
/label=oYF0019
primer_bind 805..847
/label=oYF005
CDS 814..1512
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind 814..847
/label=oYF0011
primer_bind 814..838
/label=oLZ146 GNCR1-N F
primer_bind complement(1491..1547)
/label=oYF0020
primer_bind complement(1491..1542)
/label=oYF006
primer_bind complement(1491..1513)
/label=oYF0012
primer_bind complement(1514..1547)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind 1527..1575
/label=oSCL1945
primer_bind 1527..1553
/label=oLZ146 GNCR1-N F
misc_feature 1529..1547
/label=linker
primer_bind 1529..1547
/label=oYF0015
primer_bind 1534..1574
/label=oSCL1947
CDS 1549..2247
/codon_start=1
/label=GNCR
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
CDS 2248..2277
/codon_start=1
/label=linker
/translation="ASGSGGGGDV"
primer_bind complement(2258..2282)
/label=oSCL1946
primer_bind 2263..2309
/label=oSCL1947
misc_feature 2279..2282
/label=linker
CDS 2284..2982
/codon_start=1
/label=GNCR-2
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind complement(2983..3032)
/label=oSCL1948
CDS 2983..3012
/codon_start=1
/label=linker
/translation="ASGSGGGGDV"
primer_bind 3001..3040
/label=oYF0015
primer_bind complement(3004..3032)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind complement(3004..3018)
/label=oYF0020
primer_bind complement(3013..3032)
/label=oSCL1946
misc_feature 3014..3018
/label=linker
primer_bind 3018..3040
/label=oSCL014
misc_feature 3019..3961
/label=NSP3
primer_bind 3466..3489
/label=oLZ072 NSP3(150-313)-F
primer_bind complement(3925..3960)
/label=oLZ049 NSP3-R
primer_bind complement(3929..3981)
/label=oYF0016
primer_bind complement(3929..3971)
/label=oLZ073 NSP3(150-313)-R
primer_bind complement(3931..3961)
/label=oSCL015
primer_bind complement(3941..3980)
/label=oSCL1942
misc_feature 3984..3989
/label=EcoRI
misc_feature 4023..4028
/label=New Feature
misc_feature 4023..4028
/label=New Feature
terminator 4057..4284
/label=bGH PA
primer_bind complement(4266..4306)
/label=oSCL1014
primer_bind complement(4266..4288)
/label=oSCL1018
repeat_region 4291..4431
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 4291..4420
/label=AAV2 ITR
rep_origin 4506..4961
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4593..4612)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4803..4824
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4978..4997)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5097..5119
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(5157..5175)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5243..5347
/gene="bla"
/label=AmpR promoter
CDS 5348..6208
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5566..5585)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6379..6967
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6868..6887
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggcctcta gactcgaggc gttgacattg attattgact agttattaat
181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
721 tctatataag cagagctctc tggctaacta ccggccaagc tggctagtaa tacgactcac
781 tatagggaga cccaagctgg ctaggccacc atggacatcg agaagctgtg caagaaggcg
841 gaagaggagg ccaaggaggc ccaggagaag gccgacgagc tgagacaacg acaccccgat
901 tcgcaggcgg cggaagacgc ggaggatctc gcaaatctgg ctgtcgccgc cgtgctgacc
961 gcttgtctgc tggcccagga gcatcccaac gccgatatcg ctaagctgtg catcaaggcc
1021 gcgagcgaag ctgcggaggc cgcgtcaaaa gccgccgagc tggcccaacg gcacccagac
1081 agtcaagcag ccagagatgc tatcaaactc gcctctcaag ccgcccgcgc cgtgatcctg
1141 gccatcatgc tcgccgcaga gaatcccaac gctgatattg caaagctatg tattaaggcg
1201 gccagcgaag ccgcagaggc tgcctcgaaa gctgcggaac ttgcacagcg gcaccccgac
1261 tcccaggccg cccgagatgc aattaaactg gccagtcagg ccgctgaggc ggtggagaga
1321 gccatctggc tggccgccga gaaccccaat gccgatatcg ccaaaaaatg catcaaagcg
1381 gcctccgaag cagccgagga ggcctctaaa gctgccgaag aggctcaaag acacccagac
1441 agccagaagg cacgcgacga gatcaaggag gcctctcaga aggccgagga ggtgaaggag
1501 agatgcaaga gcgccagcgg aagtggcgga ggaggcgacg tcgctagtga cattgagaag
1561 ctctgtaaga aagccgaaga agaggctaaa gaagcacagg agaaagctga tgagttgcgg
1621 cagcggcacc cggattcaca agcggccgaa gacgcagagg acctggccaa cctggccgta
1681 gccgcggttc tgaccgcatg tctgctcgcc caagagcatc ctaacgcaga tatcgctaag
1741 ctgtgcatca aggcagcgtc cgaggccgcc gaggccgcaa gtaaagccgc tgaattagca
1801 caaaggcatc cagacagtca ggccgcaagg gacgcaatca aactggcatc tcaggctgcc
1861 agagcggtga tccttgcaat tatgctggcc gccgaaaacc caaatgctga tatagcaaaa
1921 ctgtgcatca aagcagccag cgaagcagcg gaagccgctt ccaaggccgc tgagttggcc
1981 caaagacatc cagatagcca agcagcccgt gatgcgatca aactcgctag tcaggctgcc
2041 gaggccgttg aacgtgctat atggctggcg gccgaaaatc caaatgccga catagccaaa
2101 aagtgtatca aggccgcctc agaagctgcc gaagaggcga gcaaggccgc agaagaggcc
2161 cagcgccatc ccgattccca gaaggcccgg gatgagatca aagaagccag ccagaaagct
2221 gaggaggtca aagagcgatg taagagtgcg agcggaagtg gcggtggcgg tgacgtggct
2281 agtgatatcg agaagttgtg taaaaaagct gaggaagagg caaaagaggc ccaggaaaag
2341 gcagacgagc ttcgtcagag acatcctgat tcccaggccg cggaggatgc tgaggacctc
2401 gccaatttgg ccgtcgcagc tgtgctgacg gcctgcctcc ttgcgcaaga acaccctaat
2461 gcggacatag ctaaactctg cattaaggcc gcctcagagg ccgcagaagc cgcttcaaaa
2521 gctgccgaac tagcacagag acatcctgac tctcaggcag ctagagacgc tattaagctt
2581 gcttctcaag ccgcccgagc cgttatcctg gccattatgc tagccgcaga gaatccgaac
2641 gccgacatcg caaaactgtg cataaaggcc gcatctgagg cagcagaggc agccagtaag
2701 gccgctgaac tcgcacagag gcaccctgat tcacaggcag cgcgggatgc tattaagtta
2761 gcttcacagg ctgccgaagc tgtggagaga gccatttggc ttgctgcgga aaatccaaat
2821 gccgacatcg ccaagaaatg catcaaagct gcctccgagg cggccgaaga agccagtaaa
2881 gctgcagagg aagctcagcg ccaccctgac tctcagaaag cgcgggacga gattaaagag
2941 gcaagccaga aagcagaaga ggtcaaagag cgctgtaaga gcgctagtgg ctccggaggc
3001 ggcggcgacg tcgctagcat gctcaagatg gagtctacac agcagatggc tagttcgatt
3061 attaacactt cttttgaagc cgcagtcgtt gctgccactt caactcttga acttatgggc
3121 attcaatatg attataatga agtttatact agagttaaga gcaaatttga ttacgtaatg
3181 gatgattcag gtgttaaaaa caatctgttg ggtaaagcgg caactattga tcaggcactg
3241 aatggaaaat ttggttcagc agcaagaaat agaaattgga tggctgatac tagaactaca
3301 gctagactag atgaagatgt gaacaaactt agaatgatgc tatcatctaa aggaattgat
3361 caaaaaatga gagtacttaa tgcatgtttc aatgtgaagc gagttccagg taaatcatca
3421 tcaataatta aatgtactag attaatgaga gataagattg aacgtggtga agtcgaagtg
3481 gatgattcgt ttgttgaaga aaaaatggaa gttgacacaa ttgattggaa atctagatat
3541 gaacaattgg aaaaacgatt tgaatcgctg aaacaaaggg tgaatgaaaa atatacaagt
3601 tgggtgcaaa aagcaaaaaa ggtaaatgaa aatatgtact ctctccaaaa cgttatatca
3661 caacagcaga gccagattgc tgatttacaa aattactgta ataaattgga agttgatcta
3721 caaaataaaa ttagttcact tgtgtcttca gtcgaatggt acctgaaatc aatggaattg
3781 cctgatgaaa ttaagacaga tattgaacaa caactaaatt ccattgatgt aatcaatcca
3841 attaatgcta tagatgattt tgaatcactg atcagaaata ttatactcga ctatgacaga
3901 atatttttaa tgtttaaagg attaatgagg caatgcaact atgaatatac gtatgaatag
3961 ggatccacta gtccagtgtg gtggaattct gcagatatcc agcacagtgg cggccgctcg
4021 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
4081 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
4141 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
4201 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
4261 ctggggatgc ggtgggctct atggggccgc aggaacccct agtgatggag ttggccactc
4321 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
4381 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
4441 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
4501 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
4561 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
4621 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
4681 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
4741 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
4801 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
4861 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
4921 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
4981 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
5041 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
5101 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
5161 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
5221 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
5281 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
5341 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
5401 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
5461 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
5521 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
5581 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
5641 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
5701 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
5761 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
5821 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
5881 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
5941 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
6001 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
6061 tggaagccgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
6121 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
6181 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
6241 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
6301 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
6361 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
6421 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
6481 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
6541 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
6601 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
6661 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
6721 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
6781 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
6841 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
6901 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
6961 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
7021 tcacatgt
//
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