| 质粒名称: | pIMP1-Padc |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 90486 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pIMP1-Pthl |
| 完整质粒大小: | 4945 bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | None |
|
文献引用方法 |
|
| 材料和方法部分: | pIMP1-Padc was a gift from Sheng Yang (Addgene plasmid # 90486 ; http://n2t.net/addgene:90486 ; RRID:Addgene_90486) |
| 参考文献部分: | I-SceI-mediated scarless gene modification via allelic exchange in Clostridium. Zhang N, Shao L, Jiang Y, Gu Y, Li Q, Liu J, Jiang W, Yang S. J Microbiol Methods. 2015 Jan;108:49-60. doi: 10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15. 10.1016/j.mimet.2014.11.004 PubMed 25451462 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pIMP1-Padc 4945 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Plasmid with adc(CA_P0165) promoter for gene expression in
Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii
NCIMB8052.
ACCESSION .
VERSION .
KEYWORDS pIMP1-Padc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4945)
AUTHORS Zhang N, Shao L, Jiang Y, Gu Y, Li Q, Liu J, Jiang W, Yang S
TITLE I-SceI-mediated scarless gene modification via allelic exchange in
Clostridium.
JOURNAL J Microbiol Methods. 2015 Jan;108:49-60. doi:
10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15.
PUBMED 25451462
REFERENCE 2 (bases 1 to 4945)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4945
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(166..754)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(246..265)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(925..1785)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1548..1567
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1786..1890)
/gene="bla"
/label=AmpR promoter
primer_bind 1958..1976
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2014..2036)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2136..2155
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2349..2371
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2363..2380
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 2364..2380
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4709..4725)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4709..4725)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4722..4744)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4733..4749
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4757..4787)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4802..4823
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4940..12)
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
61 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
121 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
181 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
241 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
301 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
361 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
421 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
481 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
541 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
601 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
661 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
721 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
781 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
841 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
901 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
961 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
1021 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
1081 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
1141 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
1201 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
1261 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
1321 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
1381 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
1441 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
1501 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
1561 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
1621 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
1681 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
1741 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
1801 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
1861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
1921 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
1981 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
2041 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
2101 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
2161 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
2221 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
2281 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
2341 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcggtacccg
2401 gggatcctct agagtcgaca aaagtcacct tcctaaattt aataatgttt agcttttcta
2461 acatacttta tcttcacatt ataaatcgcc tctagtccta tttatttata tttacattat
2521 aacacatatt gtttttttaa gtaaattttt acaccatata ttaatttttt ctactagctt
2581 tcgaaaataa aagtacactt cctaaaaatt ctgcataata gatttatata aacttatgtg
2641 gaggtgtttt tactgcagcc aagcttaatc gcatttcata gattgacctc ccaataacta
2701 cgtggtgtta ttgggaggtc aatctatttc atttgcctct tgctcaaagt tcccaaattc
2761 gagtaagagg tatttttgtt tttggtcgtc gcctctcatt agtagttcag ggtttaacat
2821 taatactcca gtttttcttt ttataatatt tccttcttct aagattttaa gtgttgttat
2881 tactgtttgt agacttgttc ctgtagcttt tgctatttct cttgttgtag ctatcattgt
2941 attgttactt aagtggacat tatctaggat atagttaacg attttaagtt tttttccgcc
3001 aatcatatct aacatactta ttaattgcac tatatatgcc tttacgaagt taccagacgt
3061 ttgtttacgg tataacttgt ctacctctat gacttctcca ctttcttcgt ctatgagcct
3121 ctgagagcct ttatagactg ttccatatct ttctttcatc tttttctcac tccttatttt
3181 aaactattct aactatatca taactgttct aaaaaaaaaa gaacatttgt taaaagaaat
3241 tagaacaaaa tgagtgaaaa attagaacaa acaaattcct tataaacctt atcatctcaa
3301 cctatattaa gattttacct agttgaatct tcttttctat ataaagcgtc ggagcatatc
3361 agggggttat ctaacgtaaa tgctaccctt cggctcgctt tcgctcggca ttgacgtcag
3421 atactgcacc ccctgaaccc ccatgctcca acagcaaaaa ggaaactttt tgctgctttt
3481 ccgacgctta ttcgcttcgc tcatatttat atagaaaaga agtgaatgcg caaaagacat
3541 aatcgattca caaaaaatag gtacacgaaa aacaagttaa gggatgcagt ttatgcatcc
3601 cttaacttac ttattaaata atttatagct attgaaaaga gataagaatt gttcaaagct
3661 aatattgttt aaatcgtcaa ttcctgcatg ttttaaggaa ttgttaaatt gattttttgt
3721 aaatattttc ttgtattctt tgttaaccca tttcataacg aaataattat acttctgttt
3781 atctttgtgt gatattcttg atttttttct atttaatctg ataagtgagc tattcacttt
3841 aggtttagga tgaaaatatt ctcttggaac catacttaat atagaaatat caacttctgc
3901 cattaaaaat aatgccaatg agcgttttgt atttaataat cttttagcaa acccgtattc
3961 cacgattaaa taaatctcat cagctatact atcaaaaaca attttgcgta ttatatccgt
4021 acttatgtta taaggtatat taccaaatat tttataggat tggtttttag gaaatttaaa
4081 ctgcaatata tccttgttta aaacttggaa attatcgtga tcaacaagtt tattttctgt
4141 agttttgcat aatttatggt ctatttcaat ggcagttacg aaattacacc tctgtactaa
4201 ttcaagggta aaatgccctt ttcctgagcc gatttcaaag atattatcat gttcatttaa
4261 tcttatattt gtcattattt tatctatatt atgttttgaa gtaataaagt tttgactgtg
4321 ttttatattt ttctcgttca ttataaccct ctttattttt tcctccttat aaaattagta
4381 taattatagc acgagctctg ataaatatga acatgatgag tgatcgttaa atttatactg
4441 caatctgatg cgattattga ataaaagata tgagagattt atctagtttc tttttttaca
4501 agaaaaaaga aagttcttaa aggttttata cttttggtcg tagagcacac ggtttaacga
4561 cttaattacg aagtaaataa gtctagtgtg ttagacttta atgttttttt aaggcattag
4621 tgcatttaag cgtcagagca tggctttatg ccgagaaaac tattggttgg aatggcgtgt
4681 gtgttagcca aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
4741 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
4801 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
4861 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
4921 ttgggcgctc ttccgcttcc tcgct
//
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