| 质粒名称: | pAAV-AscI-CAG-H2B-NeIle |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000343 |
| 产品用途: | Nuclear expression of green Nelle Isoleucin sensor in mammalian cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-CAG |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | H2B, Nelle |
| 物种来源: | Human, Synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
NeIle, a Genetically Encoded Indicator for Branched-Chain Amino Acids Based on mNeonGreen Fluorescent Protein and LIVBP Protein
Asanova A.N., Subach O.M., Myachina S.A., Evteeva M.A., Gunitseva N.M., Borisova A.A., Patrushev M.V., Vlaskina A.V., Nikolaeva A.Y., Yang L., Gabdulkhakov A., Dronova E., Samygina V.R., Xiao X., Zhao H., Piatkevich K.D., Subach F.V.
ACS Sensors 9, 10, 5135–5147 (2024)
|
pAAV-AscI-CAG-H2B-NeIle
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-AscI-CAG-H2B-NeIle载体质粒基本信息
pAAV-AscI-CAG-H2B-NeIle质粒图谱载体图谱和pAAV-AscI-CAG-H2B-NeIle载体序列质粒序列多克隆位点信息
pAAV-AscI-CAG-H2B-NeIle质粒载体简介
pAAV-AscI-CAG-H2B-NeIle质粒序列载体序列
LOCUS pAAV-AscI-CAG-H2B-NeIle 6934 bp DNA circular SYN 15-OCT-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAAV-AscI-CAG-H2B-NeIle
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6934)
AUTHORS Vax
TITLE Direct Submission
JOURNAL Exported Jul 7, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6934
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
misc_feature 2661..2691
/label=AscI-OxE insert
/label=AscI-OxE\insert
/note="/vntifkey=21"
misc_feature 2692..2935
/label=CMV enhancer
/label=CMV\enhancer
/note="/vntifkey=21"
misc_feature 2942..3219
/label=chicken b-actin promoter
/label=chicken\b-actin\promoter
/note="/vntifkey=21"
CDS 3646..4023
/codon_start=1
/label=H2B
/translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
GELAKHAVSEGTKAITKYTSAK"
CDS 4030..5817
/codon_start=1
/label=NeIle
/note="/vntifkey=21"
/translation="MEDIKVAVVGAMSAGADWRISKEACPNGKTLISTLKWSYTTGDGK
RYRSTARTTFTFAKPMAANYLKNQPMYVFRRTELKHSKTEMDFKEWQKAFTDVVGMDEL
YKVDGGSGGTGMVSEGEVDNMASLPATHELHIFGSINGVGFDMVGQGSGNPNVGYEELN
LKSTKGELQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTVRFEDGAS
LTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAFHIGPVTQYGDQEFTGAEQAV
ADINARGGIKGNKLQIVKYDDACDPKQAVAVANRVVNDGIKYVIGHLCSSSAQPASDIY
EDAGILMITPAATAPELTARGYQLILRTTGLDSDQGLTAAKYILEKVKPQRIAIVHDKQ
QYGEGLARAVQGGLKKGNANVVFFDGITAGEKDFSTLVARLKKENIDFVYYGGYHPEMG
QILRQARAAGLKTQFMGPEGVANVSLSNIADESAEGLLVTKPKNYDQVPANKHIVDAVK
AKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDTVMGPLTWDEKGDLK
GFEFGVFDWHANGTATDAK"
misc_feature 5836..6424
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal 6467..6588
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature join(6934,1..940)
/label=ColE1
/note="ColE1"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
3241 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
3301 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
3361 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
3421 ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct gccttcgggg
3481 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
3541 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
3601 gctgtctcat cattttggca aagaattgga tccaccggtg acaccatgcc agagccagcg
3661 aagtctgctc ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa
3721 ggcggcaaga agcgcaagcg cagccgcaag gagagctatt ccatctatgt gtacaaggtt
3781 ctgaagcagg tccaccctga caccggcatt tcgtccaagg ccatgggcat catgaattcg
3841 tttgtgaacg acattttcga gcgcatcgca ggtgaggctt cccgcctggc gcattacaac
3901 aagcgctcga ccatcacctc cagggagatc cagacggccg tgcgcctgct gctgcctggg
3961 gagttggcca agcacgccgt gtccgagggt actaaggcca tcaccaagta caccagcgct
4021 aagagatcta tggaagatat taaagtcgcg gtcgtgggcg caatgtccgc tggtgctgac
4081 tggcgtatat ccaaggaggc ttgccccaat ggtaagactc ttatttccac acttaagtgg
4141 tcttatacca ccggcgacgg caaacgctac cggtctactg ctcgcaccac attcacattc
4201 gccaaaccaa tggccgcaaa ttacctcaaa aatcagccga tgtatgtctt ccggagaaca
4261 gagctgaaac attctaagac cgaaatggat tttaaagagt ggcaaaaggc ctttacggac
4321 gtggtgggca tggacgagct gtacaaggtg gatggcggca gcggtggcac cggcatggtc
4381 tcagagggag aggtggacaa catggctagt ctgcctgcta cccacgaact gcatattttt
4441 ggctccataa atggcgtcgg tttcgacatg gtaggccaag gctccggtaa tccaaacgtc
4501 ggttatgagg agctgaacct gaagtccacc aagggcgaac tgcagttcag tccctggatc
4561 ctggtgccgc acattgggta tgggtttcat cagtatcttc cctacccgga cgggatgtcg
4621 ccatttcagg ctgcaatggt cgacgggtcc ggatatcagg tgcaccgcac tgtgcggttt
4681 gaggacggtg cttcactgac cgtgaattac agatacacct acgaaggatc acatatcaaa
4741 ggggaagctc aggtgaaggg cactgggttc ccggcagacg gccctgtgat gacaaattcc
4801 ctgacagcct ttcatattgg tccggtgacc cagtacggtg accaggagtt taccggcgca
4861 gagcaggcgg ttgcggatat caacgctaga ggcggcatta agggcaacaa actgcaaatc
4921 gtaaaatatg acgatgcctg tgacccgaaa caggctgttg cggtggcgaa cagagtcgtt
4981 aacgacggca ttaaatatgt gattggtcac ctctgttctt catcagcgca gcctgcgtct
5041 gacatctacg aagacgcagg cattttaatg atcaccccag cggcaaccgc gccggagctg
5101 accgcccgtg gctatcagct gatcctgcgc accaccggcc tggactccga ccaggggctg
5161 acggcggcga aatatattct tgagaaagtg aaaccgcagc gtattgctat cgttcacgac
5221 aaacagcagt acggcgaagg tctggcgcga gcggtgcagg gtggcctgaa gaaaggcaat
5281 gcaaacgtgg tgttctttga tggcatcacc gccggggaaa aagatttctc aacgctggtg
5341 gcgcgtctga aaaaagagaa tatcgacttc gtttactacg gcggttatca cccggaaatg
5401 gggcaaatcc tgcgtcaggc acgcgcggca gggctgaaaa ctcagtttat ggggccggag
5461 ggtgtggcta acgtttcgct gtctaacatt gcggacgaat cagcggaagg gctgctggtg
5521 accaagccga agaactacga tcaggttccg gcgaacaaac acattgttga cgcagtcaaa
5581 gcgaaaaaac aggacccaag tggcgcattc gtttggacca cctacgccgc gctgcaatct
5641 ttgcaggcgg gcctgaatca gtctgacgat ccggctgaaa tcgccaaata cctgaaagcg
5701 aactccgtgg ataccgtaat gggaccgctg acctgggatg agaaaggcga tctgaaaggc
5761 tttgagttcg gcgtatttga ctggcacgcc aacggcacgg cgaccgatgc gaagtaaact
5821 agtaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
5881 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
5941 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
6001 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
6061 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
6121 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
6181 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
6241 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
6301 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
6361 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
6421 ccgcatcgat accgtcgacc cgggcggccg cttcgagcag acatgataag atacattgat
6481 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
6541 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
6601 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
6661 aacctctaca aatgtggtaa aatcgataag gatcttccta gagcatggct acgtagataa
6721 gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc
6781 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
6841 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcattaatg aatcggccaa
6901 cgcgcgggga gaggcggttt gcgtattggg cgct
//
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