pSL894

LOCUS       pSL894                8036 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..191
                     /label=182-WKG-596-F
     misc_feature    232
                     /label=CMV
     promoter        245..441
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     regulatory      467..476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    473..535
                     /label=Igk secretion signal
     CDS             542..1129
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     misc_feature    1136..1879
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     primer_bind     complement(1203..1225)
                     /label=186-WKG-603-R
     misc_feature    1409..1411
                     /label=F93A
                     /note="F93A"
     misc_feature    1857..1879
                     /label=EpoR-D1_D2_TM
                     /note="EpoR-D1_D2_TM"
     misc_feature    1857..1879
                     /label=transmembrane
                     /note="transmembrane"
     misc_feature    1880..3592
                     /label=VEGFR
                     /note="VEGFR"
     polyA_signal    3633..3857
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3903..4331
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4202..4221
                     /label=189-WKG-612-F
     promoter        4345..4674
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4741..5535
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4785..4812)
                     /label=188-WKG-610-R
     polyA_signal    5709..5830
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5875..5895)
                     /label=M13-rev
     primer_bind     complement(5879..5895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(5901..5923)
                     /label=LacO
     protein_bind    5903..5919
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5927..5957)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(5928..5957)
                     /label=lac
     protein_bind    5972..5993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6099..7039
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(7040..7900)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7901..8005)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgatctgc
      241 gatctgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      301 ctaactccgc ccagttccgc ccattctccg ccccatcgct gactaatttt ttttatttat
      361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      421 ggaggcctag gcttttgcaa aaagctacga atcgcgctag tggccggcca ccatggagac
      481 cgatacctta ttactgtggg tgcttcttct gtgggttccc ggtagcaccg gagacggatc
      541 caagaagaag ggcagcgtgg tgatcgtggg cagaatcaac ctgagcggcg acaccgccta
      601 cagccagcag accagaggcc tggagggctg ccaggagacc agccagaccg gcagagacaa
      661 gaaccaggtg gagggcgagg tgcaggtggt gagcaccgcc acccagagct tcctggccac
      721 cagcatcaac ggcgtgctgt ggaccgtgta ccacggcgcc ggcaccagaa ccatcgccag
      781 ccccaagggc cccgtgaccc agatgtacac gaacgtggac aaggacttag tgggctggca
      841 ggccccccaa ggcagcagaa gcctgacccc ctgcacctgc ggcagcagcg acctgtacct
      901 ggtgaccaga cacgccgacg tgatccccgt gagaagaaga ggcgacagca gaggcagcct
      961 gctgagcccc agacccatca gctatctgaa aggctcaagc ggcggccccc tgttgtgtcc
     1021 cgccggccat gccgtgggca tcttcagagc cgccgtgagc accagaggcg tggccaaggc
     1081 cgtggacttc atccccgtgg agagcctgga gaccaccatg agaagccccg aattcgcacc
     1141 ttcacccagc ctcccggacc ccaagtttga gagcaaagcg gccctgctgg catcccgggg
     1201 ctccgaagaa cttctgtgct tcacccaacg cttggaagac ttggtgtgtt tctgggagga
     1261 agcggcgagc tccgggatgg acttcaacta cagcttctca taccagctcg agggtgagtc
     1321 acgaaagtca tgtagcctgc accaggctcc caccgtccgc ggctccgtgc gtttctggtg
     1381 ttcactgcca acagcggaca catcgagtgc tgtgccgctg gagctgcagg tgacggaggc
     1441 gtccggttct cctcgctatc accgcatcat ccatatcaat gaagtagtgc tcctggacgc
     1501 ccccgcgggg ctgctggcgc gccgggcaga agagggcagc cacgtggtgc tgcgctggct
     1561 gccacctcct ggagcaccta tgaccaccca catccgatat gaagtggacg tgtcggcagg
     1621 caaccgggca ggagggacac aaagggtgga ggtcctggaa ggccgcactg agtgtgttct
     1681 gagcaacctg cggggcggga cgcgctacac cttcgctgtt cgagcgcgca tggccgagcc
     1741 gagcttcagc ggattctgga gtgcctggtc tgagcccgcg tcactactga ccgctagcga
     1801 cctggaccct ctcatcttga cgctgtctct cattctggtc ctcatctcgt tgttgctgac
     1861 ggttctggcc ctgctgtccc tacggaccgt taagcgggcc aatggagggg aactgaagac
     1921 aggctacttg tccatcgtca tggacccaga tgaactccca ttggatgaac attgtgaacg
     1981 actgccttat gatgccagca aatgggagtt ccccagagac cggctgaagc taggtaagcc
     2041 tcttggccgt ggtgcctttg gccaagtgat tgaagcagat gcctttggaa ttgacaagac
     2101 agcaacttgc aggacagtag cagtcaaaat gttgaaagaa ggagcaacac acagtgagca
     2161 tcgagctctc atgtctgaac tcaagatcct cattcatatt ggtcaccatc tcaatgtggt
     2221 caaccttcta ggtgcctgta ccaagccagg agggccactc atggtgattg tggagttctg
     2281 caaatttgga aacctgtcca cttacctgag gagcaagaga aatgaatttg tcccctacaa
     2341 gaccaaaggg gcacgattcc gtcaagggaa agactacgtt ggagcaatcc ctgtggatct
     2401 gaaacggcgc ttggacagca tcaccagtag ccagagctca gccagctctg gatttgtgga
     2461 ggagaagtcc ctcagtgatg tagaagaaga ggaagctcct gaagatctgt ataaggactt
     2521 cctgaccttg gagcatctca tctgttacag cttccaagtg gctaagggca tggagttctt
     2581 ggcatcgcga aagtgtatcc acagggacct ggcggcacga aatatcctct tatcggagaa
     2641 gaacgtggtt aaaatctgtg actttggctt ggcccgggat atttataaag atccagatta
     2701 tgtcagaaaa ggagatgctc gcctcccttt gaaatggatg gccccagaaa caatttttga
     2761 cagagtgtac acaatccaga gtgacgtctg gtcttttggt gttttgctgt gggaaatatt
     2821 ttccttaggt gcttctccat atcctggggt aaagattgat gaagaatttt gtaggcgatt
     2881 gaaagaagga actagaatga gggcccctga ttatactaca ccagaaatgt accagaccat
     2941 gctggactgc tggcacgggg agcccagtca gagacccacg ttttcagagt tggtggaaca
     3001 tttgggaaat ctcttgcaag ctaatgctca gcaggatggc aaagactaca ttgttcttcc
     3061 gatatcagag actttgagca tggaagagga ttctggactc tctctgccta cctcacctgt
     3121 ttcctgtatg gaggaggagg aagtatgtga ccccaaattc cattatgaca acacagcagg
     3181 aatcagtcag tatctgcaga acagtaagcg aaagagccgg cctgtgagtg taaaaacatt
     3241 tgaagatatc ccgttagaag aaccagaagt aaaagtaatc ccagatgaca accagacgga
     3301 cagtggtatg gttcttgcct cagaagagct gaaaactttg gaagacagaa ccaaattatc
     3361 tccatctttt ggtggaatgg tgcccagcaa aagcagggag tctgtggcat ctgaaggctc
     3421 aaaccagaca agcggctacc agtccggata tcactccgat gacacagaca ccaccgtgta
     3481 ctccagtgag gaagcagaac ttttaaagct gatagagatt ggagtgcaaa ccggtagcac
     3541 agcccagatt ctccagcctg actcggggac cacactgagc tctcctcctg tttaatctag
     3601 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     3661 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3721 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3781 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     3841 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     3901 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3961 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4021 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4081 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4141 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4201 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4261 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4321 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4381 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4441 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4501 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4561 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     4621 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     4681 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     4741 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     4801 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     4861 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     4921 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     4981 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     5041 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     5101 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     5161 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     5221 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     5281 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5341 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5401 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5461 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5521 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     5581 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5641 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     5701 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5761 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5821 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     5881 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5941 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6001 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6061 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7321 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7621 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7681 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7741 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7801 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7861 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7921 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7981 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//