| 质粒名称: | pEXPYR |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 70030 |
| 存储实验室: | Rolf Prade |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 |
| 载体出品公司: | New England Bioscienes |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 合成生物学表达 |
| 筛选标记: | 博莱霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | A. niger glucoamylase promoter (glaAp)4 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pEXPYR was a gift from Rolf Prade (Addgene plasmid # 70030 ; http://n2t.net/addgene:70030 ; RRID:Addgene_70030) |
| 参考文献部分: | High-yield secretion of multiple client proteins in Aspergillus. Segato F, Damasio AR, Goncalves TA, de Lucas RC, Squina FM, Decker SR, Prade RA. Enzyme Microb Technol. 2012 Jul 15;51(2):100-6. doi: 10.1016/j.enzmictec.2012.04.008. Epub 2012 May 2. 10.1016/j.enzmictec.2012.04.008 PubMed 22664194 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEXPYR 6705 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION High yield protein secretion in Aspergillus nidulans.
ACCESSION .
VERSION .
KEYWORDS pEXPYR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6705)
AUTHORS Segato F, Damasio AR, Goncalves TA, de Lucas RC, Squina FM, Decker
SR, Prade RA
TITLE High-yield secretion of multiple client proteins in Aspergillus.
JOURNAL Enzyme Microb Technol. 2012 Jul 15;51(2):100-6. doi:
10.1016/j.enzmictec.2012.04.008. Epub 2012 May 2.
PUBMED 22664194
REFERENCE 2 (bases 1 to 6705)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6705
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 3359..3733
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
terminator 3818..4065
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(3834..3852)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
primer_bind complement(4084..4100)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4084..4100)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4097..4119)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4108..4124
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4132..4162)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4177..4198
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4315..4332)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4486..5074)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4566..4585)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5542..5921)
/label=ISS
/note="immunostimulatory sequence from the AmpR gene;
contains unmethylated CpG dinucleotides in the context of
5'-AACGTT-3' (Sato et al., 1996)"
primer_bind 5868..5887
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6106..6210)
/gene="bla"
/label=AmpR promoter
primer_bind 6278..6296
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(6334..6356)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 6456..6475
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6669..6691
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6683..6700
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6684..6700
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 aattcgagct ctcccctgac cttccgaact ggtcgtacct ggcgacctat gactatggca
61 ccccagttct ggggaccttc cacggaagtg acctgctgca ggtgttctat gggatcaagc
121 caaactatgc agctagttct agccacacgt actatctgag ctttgtgtat acgctggatc
181 cgaactccaa ccggggggag tacattgagt ggccgcagtg gaaggaatcg cggcagttga
241 tgaatttcgg agcgaacgac gccaggtctc cttacggatg attaccgcaa cgggacatat
301 gagttcatcc tgcagaatac cgcggcgttc cacatctgat gccattggcg gaggggtccg
361 gacggtcagg aacttagcct tatgagatga atgatggacg tgtctggcct cggaaaagga
421 tatatgggga tcatgatagt actagccata ttaatgaagg gcatatacca cgcgttggac
481 ctgcgttata gcttcccgtt agttatagta ccatcgttat accagccaat caagtcacca
541 cgcacgaccg gggacggcga atccccggga attgaaagaa attgcatccc aggccagtga
601 ggccagcgat tggccacctc tccaaggcac agggccattc tgcagcgctg gtggattcat
661 cgcaatttcc cccggcccgg cccgacaccg ctataggctg gttctcccac accatcggag
721 attcgtcgcc taatgtctcg tccgttcaca agctgaagag cttgaagtgg cgagatgtct
781 ctgcaggaat taattcaagc tagatgctaa gcgatattgc atggcaatat gtgttgatgc
841 atgtgcttct tccttcagct tcccctcgtg cagatgaggt ttggctataa attgaagtgg
901 ttggtcgggg ttccgtgagg ggctgaagtg cttcctccct tttagacgca actgagagcc
961 tgagcttcac ccccagcatc attacacctc agcaatgtcg ttccgatctc tactcgccct
1021 gagcggcctc gtctgcacag ggttggcaaa tgtgatttcc aagcgcggcc gctctagaga
1081 tatctagtga tttaatagct ccatgtcaac aagaataaaa cgcgtttcgg gtttacctct
1141 tccagataca gctcatctgc aatgcattaa tgcattggac ctcgcaaccc tagtacgccc
1201 ttcaggctcc ggcgaagcag aagaatagct tagcagagtc tattttcatt ttcgggagac
1261 gagatcaagc agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc
1321 tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg
1381 atagcttgac tatgaaaatt ccgtcaccag cccctgggtt cgcaaagata attgcactgt
1441 ttcttccttg aactctcaag cctacaggac acacattcat cgtaggtata aacctcgaaa
1501 atcattccta ctaagatggg tatacaatag taaccatgca tggttgccta gtgaatgctc
1561 cgtaacaccc aatacgccgg ccgaaacttt tttacaactc tcctatgagt cgtttaccca
1621 gaatgcacag gtacactgca gggatccccc gggctgcagg aattcgatat caagcttgca
1681 tgcctgcagc aattcgacga aatccgcgac gtcttctcca agtgtggtgt tatcgccgag
1741 gagatcgata gttcttgtcc ttcaagtgct tgaccaatat gtttctgtta acgcgccgca
1801 gacaatgctc tctatcctgg tggcaggcgt caagtaccca gaggcagcag cgggcttagg
1861 agcggcctgg gttgttctcc gcaccctcta catgctgggc tatatttata gcgacaagcc
1921 gaacggcacc ggcaggtaca atggttcgct gtacttgctt gcgcaagcgg gtctttgggg
1981 attgagcgca tttggtgttg caaaggattt gatgtaaatg tagtcgacat cttagcacag
2041 aggggagagt tgataaaatg tggtctgttt gaatgatagt cgggttcgtg acctatattc
2101 gtgatagtgg agataggtct gcgcctatct tatcgggccg gagcaaaaat tccaccgcag
2161 cggggtgagt tttcgttata cagccatccc acttccagct tcaaattgtc agtttaatcc
2221 agcccaattc aatcattgga gaaccgccat catgtcttcg aagtcccacc tcccctacgc
2281 aattcgcgca accaaccatc ccaacccttt aacatctaaa ctcttctcca tcgccgagga
2341 gaagaaaacc aacgtcaccg tctccgcaga cgttactact tccgccgagc tcctcgatct
2401 tgctgaccgt acatcctgca ccaatgcccc tccaggataa caaatagctg atgcgtagtg
2461 agtacaggcc taggccccta tatcgcagtt ctgaaaaccc acatcgacat cctcaccgat
2521 ctcaccccgt cgaccctttc ctcgctccaa tccctcgcga caaagcacaa cttcctcatc
2581 tttgaggacc gcaagttcat cgacatcggc aacaccgtgc aaaagcagta ccacggtggc
2641 gctctccgca tctccgaatg ggcacacatc atcaactgcg ccatcctgcc gggcgaaggg
2701 atcgtcgagg ccctcgcaca gacaaccaag tctcctgact ttaaagacgc gaatcaacga
2761 ggtctcctga ttcttgccga gatgacgagt aagggatctc ttgcgacagg ggagtcacag
2821 gcacgctcgg ttgagtacgc gcggaagtat aaggggtttg tgatgggatt cgtgagtaca
2881 agggcgttga gtgaggtgct gcccgaacag aaagaggaga gcgaggattt tgtcgtcttt
2941 acgactgggg tgaatctgtc ggataagggg gataagctgg ggcagcagta tcagacacct
3001 gggtcggcgg ttgggcgagg tgcggacttt atcattgcgg gtaggggcat ctataaggcg
3061 gacgatccag tcgaggcggt tcagaggtac cgggaggaag gctggaaagc ttacgagaaa
3121 agagttggac tttgagtgtg agtggaaatg tgtaacggta ttgactaaaa ggggagttct
3181 tgtccttcaa gtgcttgacc aatatgtttc tgttctcgac tgtctttccc cagtctcctc
3241 ccaacaactt cccgcccatc tactccctcc ggcctcgccg tctctcatac cccttcgacc
3301 cttttcaata ccttcaaccc cttcaatcta cccggcagaa ttctctagta gaggatccat
3361 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
3421 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
3481 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
3541 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
3601 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
3661 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
3721 ggagcaggac tgaccgacgc cgaccaacac cgccggtccg acgcggcccg acgggtccga
3781 ggcctcggag atccgtcccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc
3841 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
3901 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata
3961 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa
4021 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcaagct tggcgtaatc
4081 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
4141 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
4201 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
4261 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
4321 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
4381 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
4441 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
4501 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
4561 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
4621 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
4681 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
4741 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
4801 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
4861 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
4921 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
4981 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
5041 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
5101 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
5161 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
5221 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
5281 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
5341 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
5401 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
5461 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
5521 ttcgccagtt aatagttgcg caacgttgtt gccatttgct acaggcatcg tggtgtcacg
5581 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
5641 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
5701 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
5761 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
5821 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
5881 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
5941 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
6001 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
6061 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
6121 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
6181 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
6241 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
6301 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
6361 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
6421 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
6481 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
6541 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
6601 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
6661 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaatt
//
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