| 质粒名称: | pLZ535 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000634 |
| 产品用途: | Mammalian cAMP-responsive expression vector for SEAP and mINS (PCRE-SEAP-P2A-mINS-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
pCRE
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: |
SEAP, Insulin
|
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLZ535
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLZ535载体质粒基本信息
pLZ535质粒图谱载体图谱和pLZ535载体序列质粒序列多克隆位点信息
pLZ535质粒载体简介
pLZ535质粒序列载体序列
LOCUS pLZ535 5294 bp DNA circular SYN 12-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5294)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Jul 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5294
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 9..162
/label=Transcription blocker
/label=Transcription\blocker
primer_bind 111..130
/label=RVP3
misc_feature 196..282
/label=CRE reg_Regulatory_Seq
misc_feature 207..214
/label=CRE
misc_feature 243..250
/label=CRE*
misc_feature 265..272
/label=CRE
promoter 289..435
/label=P_TAL
primer_bind 424..464
/label=oLH481 F
primer_bind 444..458
/label=Primer 1
CDS 457..1953
/codon_start=1
/product="secreted alkaline phosphatase from human
placenta"
/label=SEAP
/note="The natural enzyme has a C-terminal transmembrane
sequence that is replaced by a GPI anchor."
/translation="MLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPAQ
TAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHV
PDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTT
TRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYM
FRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLM
GLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAY
RALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARD
RKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARG
PQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
primer_bind 458..473
/label=Primer 7
primer_bind complement(1988..2007)
/label=Primer 8
CDS 2011..2067
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 2068..2397
/label=mINS
primer_bind 2068..2087
/label=9
primer_bind complement(2376..2423)
/label=oLH482
primer_bind complement(2381..2402)
/label=Primer 2
primer_bind complement(2381..2401)
/label=Primer 9
primer_bind complement(2382..2400)
/label=mINS-R
primer_bind complement(2382..2398)
/label=Primer 2
primer_bind complement(2403..2422)
/label=Primer 8
misc_feature 2407..2425
/label=SEAP
polyA_signal 2417..2608
/label=SV40 late polyA
polyA_signal 2426..2547
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2429..2448)
/label=PCMV-R
primer_bind complement(2437..2456)
/label=EBV-R
primer_bind complement(2445..2464)
/label=PCMV-myc-R-bd
primer_bind complement(2447..2466)
/label=pCI-neo-R
primer_bind 2450..2470
/label=PSG5-R(SV40-R)
primer_bind 2514..2535
/label=PFastBac1-R
primer_bind complement(2522..2543)
/label=CMV(-1063)
primer_bind complement(2529..2547)
/label=SV40-PolyA
primer_bind complement(2706..2725)
/label=RVP4
primer_bind complement(2727..2746)
/label=XbaI.R
misc_feature 2784..3724
/label=ColE1
/note="ColE1"
primer_bind complement(2795..2812)
/label=L4440
primer_bind complement(2844..2867)
/label=PTRE-R（A3254）
primer_bind complement(3046..3065)
/label=PBR322ori-F
primer_bind 3046..3065
/label=PBR322ori-R
primer_bind 3604..3627
/label=BlaRV
CDS complement(3725..4585)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3809..3832)
/label=BlaM1
primer_bind 3953..3972
/label=anbian-R
primer_bind complement(4145..4164)
/label=anbian-F
promoter complement(4586..4690)
/label=AmpR promoter
primer_bind 4621..4642
/label=163-F
rep_origin 4717..5172
/label=M13 origin
primer_bind complement(4804..4823)
/label=F1ori-R
primer_bind 5014..5035
/label=F1ori-F
CDS complement(5174..5242)
/label=LacZ alpha
primer_bind 5183..5204
/label=PMD-F
primer_bind 5222..5240
/label=1300-R
ORIGIN
1 gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
61 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
121 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctatcgatag gtaccgagct
181 cttacgcgtg ctagcgcacc agacagtgac gtcagctgcc agatcccatg gccgtcatac
241 tgtgacgtct ttcagacacc ccattgacgt caatgggaga acagatctgc cgccccgact
301 gcatctgcgt gttcgaattc gccaatgaca agacgctggg cggggtttgt gtcatcatag
361 aactaaagac atgcaaatat atttcttccg gggacaccgc cagcaaacgc gagcaacggg
421 ccacggggat gaagcagaag cttagaattc gccaccatgc tgctgctgct gctgggcctg
481 aggctacagc tctccctggg catcatccca gttgaggagg agaacccgga cttctggaac
541 cgcgaggcag ccgaggccct gggtgccgcc aagaagctgc agcctgcaca gacagccgcc
601 aagaacctca tcatcttcct gggcgatggg atgggggtgt ctacggtgac agctgccagg
661 atcctaaaag ggcagaagaa ggacaaactg gggcctgaga tacccctggc catggaccgc
721 ttcccatatg tggctctgtc caagacatac aatgtagaca aacatgtgcc agacagtgga
781 gccacagcca cggcctacct gtgcggggtc aagggcaact tccagaccat tggcttgagt
841 gcagccgccc gctttaacca gtgcaacacg acacgcggca acgaggtcat ctccgtgatg
901 aatcgggcca agaaagcagg gaagtcagtg ggagtggtaa ccaccacacg agtgcagcac
961 gcctcgccag ccggcaccta cgcccacacg gtgaaccgca actggtactc ggacgccgac
1021 gtgcctgcct cggcccgcca ggaggggtgc caggacatcg ctacgcagct catctccaac
1081 atggacattg acgtgatcct aggtggaggc cgaaagtaca tgtttcgcat gggaacccca
1141 gaccctgagt acccagatga ctacagccaa ggtgggacca ggctggacgg gaagaatctg
1201 gtgcaggaat ggctggcgaa gcgccagggt gcccggtatg tgtggaaccg cactgagctc
1261 atgcaggctt ccctggaccc gtctgtgacc catctcatgg gtctctttga gcctggagac
1321 atgaaatacg agatccaccg agactccaca ctggacccct ccctgatgga gatgacagag
1381 gctgccctgc gcctgctgag caggaacccc cgcggcttct tcctcttcgt ggagggtggt
1441 cgcatcgacc atggtcatca tgaaagcagg gcttaccggg cactgactga gacgatcatg
1501 ttcgacgacg ccattgagag ggcgggccag ctcaccagcg aggaggacac gctgagcctc
1561 gtcactgccg accactccca cgtcttctcc ttcggaggct accccctgcg agggagctcc
1621 atcttcgggc tggcccctgg caaggcccgg gacaggaagg cctacacggt cctcctatac
1681 ggaaacggtc caggctatgt gctcaaggac ggcgcccggc cggatgttac cgagagcgag
1741 agcgggagcc ccgagtatcg gcagcagtca gcagtgcccc tggacgaaga gacccacgca
1801 ggcgaggacg tggcggtgtt cgcgcgcggc ccgcaggcgc acctggttca cggcgtgcag
1861 gagcagacct tcatagcgca cgtcatggcc ttcgccgcct gcctggagcc ctacaccgcc
1921 tgcgacctgg cgccccccgc cggcaccacc gacgccgcgc acccgggtta ctctagagtc
1981 ggggcggccg gccgcttcga gcagacagga gcaaccaact tttccctgct gaagcaggca
2041 ggcgacgtgg aggagaatcc tggacccatg gccctgtgga tgcgcttcct gcccctgctg
2101 gccctgctcg tcctctggga gcccaagcct gcccaggctt ttgtcaaaca gcacctttgt
2161 ggtcctcacc tggtggaggc tctgtacctg gtgtgtgggg aacgtggttt cttctacaca
2221 cccaagtccc gtcgtaaaag ggaggacccg caagtgccac aactggagct gggtggaggc
2281 ccggaggccg gggatcttca gaccttggca ctggaggttg cccggcagaa gcgtggcatt
2341 gtggatcagt gctgcaccag catctgctcc ctctaccaac tggagaacta ctgcaactga
2401 ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
2461 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
2521 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
2581 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
2641 atcgataagg atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
2701 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
2761 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
2821 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
2881 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
2941 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
3001 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
3061 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
3121 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
3181 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
3241 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
3301 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
3361 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
3421 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
3481 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
3541 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
3601 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
3661 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
3721 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
3781 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
3841 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
3901 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
3961 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
4021 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
4081 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
4141 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
4201 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
4261 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
4321 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
4381 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
4441 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
4501 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
4561 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
4621 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
4681 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
4741 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
4801 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
4861 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
4921 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
4981 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
5041 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
5101 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
5161 cgtttacaat ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
5221 gggcctcttc gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac
5281 gggaggtact tgga
//
