pLZ531

LOCUS       pLZ531               8340 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    895..900
                     /label=NheI
     misc_feature    911..915
                     /label=New Feature
     misc_feature    911..915
                     /label=New Feature
     misc_feature    924..1162
                     /label=GLP1-Fc
     CDS             1065..1157
                     /codon_start=1
                     /label=GLP-1
                     /translation="HGEGTFTSDVSSYLEGQAAKEFIAWLVKGRG"
     misc_feature    1068..1070
                     /label=A-G
     misc_feature    1110..1112
                     /label=G-E
     misc_feature    1152..1154
                     /label=R-G
     CDS             1158..1871
                     /codon_start=1
                     /label=Fc
                     /translation="ISAMVRSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPE
                     VTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGK
                     EYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSD
                     IAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNH
                     YTQKSLSLSPGKASGS"
     primer_bind     1158..1181
                     /label=Hwtx-F
     primer_bind     1534..1555
                     /label=203-WKG-633-F1
     CDS             1875..1931
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    1932..2000
                     /label=Secretory (IgK)
                     /label=nonstandard type:  signal
     CDS             2019..2534
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    2052..2054
                     /label=cyt-STOP (TGG->TGA)
     primer_bind     2327..2356
                     /label=201-WKG-631-F2
     misc_feature    2560..2563
                     /label=linker
     misc_feature    2580..2592
                     /label=linker
     misc_feature    2598..2616
                     /label=MS2 box
     misc_feature    2622..2631
                     /label=linker
     misc_feature    2637..2655
                     /label=MS2 box
     misc_feature    2661..2670
                     /label=linker
     misc_feature    2661..2670
                     /label=linker
     misc_feature    2669..2699
                     /label=hairpin
     misc_feature    2675..2693
                     /label=MS2 box
     misc_feature    2698..2710
                     /label=linker
     misc_feature    2709..2739
                     /label=hairpin
     misc_feature    2715..2733
                     /label=MS2 box
     misc_feature    2743..2748
                     /label=linker
     misc_feature    2757..2772
                     /label=linker
     misc_feature    2778..2781
                     /label=linker
     misc_feature    2798..2810
                     /label=linker
     misc_feature    2816..2834
                     /label=MS2 box
     misc_feature    2840..2849
                     /label=linker
     misc_feature    2855..2873
                     /label=MS2 box
     misc_feature    2879..2888
                     /label=linker
     misc_feature    2879..2888
                     /label=linker
     misc_feature    2887..2917
                     /label=hairpin
     misc_feature    2893..2911
                     /label=MS2 box
     misc_feature    2916..2928
                     /label=linker
     misc_feature    2927..2957
                     /label=hairpin
     misc_feature    2933..2951
                     /label=MS2 box
     misc_feature    2961..2966
                     /label=linker
     misc_feature    2975..2990
                     /label=linker
     misc_feature    2996..2999
                     /label=linker
     misc_feature    3016..3028
                     /label=linker
     misc_feature    3034..3052
                     /label=MS2 box
     misc_feature    3058..3067
                     /label=linker
     misc_feature    3073..3091
                     /label=MS2 box
     misc_feature    3097..3106
                     /label=linker
     misc_feature    3097..3106
                     /label=linker
     misc_feature    3105..3135
                     /label=hairpin
     misc_feature    3111..3129
                     /label=MS2 box
     misc_feature    3134..3146
                     /label=linker
     misc_feature    3145..3175
                     /label=hairpin
     misc_feature    3151..3169
                     /label=MS2 box
     misc_feature    3179..3184
                     /label=linker
     misc_feature    3193..3208
                     /label=linker
     misc_feature    3235..3247
                     /label=linker
     misc_feature    3253..3271
                     /label=MS2 box
     misc_feature    3277..3286
                     /label=linker
     misc_feature    3292..3310
                     /label=MS2 box
     misc_feature    3316..3325
                     /label=linker
     misc_feature    3316..3325
                     /label=linker
     misc_feature    3324..3354
                     /label=hairpin
     misc_feature    3330..3348
                     /label=MS2 box
     misc_feature    3353..3365
                     /label=linker
     misc_feature    3364..3394
                     /label=hairpin
     misc_feature    3370..3388
                     /label=MS2 box
     misc_feature    3398..3403
                     /label=linker
     misc_feature    3412..3427
                     /label=linker
     misc_feature    3433..3445
                     /label=linker
     misc_feature    3451..3469
                     /label=MS2 box
     misc_feature    3475..3484
                     /label=linker
     misc_feature    3490..3508
                     /label=MS2 box
     misc_feature    3514..3523
                     /label=linker
     misc_feature    3514..3523
                     /label=linker
     misc_feature    3522..3552
                     /label=hairpin
     misc_feature    3528..3546
                     /label=MS2 box
     misc_feature    3551..3563
                     /label=linker
     misc_feature    3562..3592
                     /label=hairpin
     misc_feature    3568..3586
                     /label=MS2 box
     misc_feature    3596..3601
                     /label=linker
     misc_feature    3610..3625
                     /label=linker
     misc_feature    3631..3643
                     /label=linker
     misc_feature    3649..3667
                     /label=MS2 box
     misc_feature    3673..3682
                     /label=linker
     misc_feature    3688..3706
                     /label=MS2 box
     misc_feature    3712..3721
                     /label=linker
     misc_feature    3712..3721
                     /label=linker
     misc_feature    3720..3750
                     /label=hairpin
     misc_feature    3726..3744
                     /label=MS2 box
     misc_feature    3749..3761
                     /label=linker
     misc_feature    3760..3790
                     /label=hairpin
     misc_feature    3766..3784
                     /label=MS2 box
     misc_feature    3794..3799
                     /label=linker
     misc_feature    3808..3823
                     /label=linker
     misc_feature    3830..3905
                     /label=HHR Env140_nutR_H6
     misc_feature    complement(3838..3846)
                     /label=Stem III
     misc_feature    3849..3864
                     /label=Stem I
     terminator      3873..3887
                     /label=nutR-boxB
     misc_feature    3891..3899
                     /label=Stem III
     polyA_signal    3937..4161
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4207..4635
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4221..4245)
                     /label=184-WKG-599-R
     promoter        4649..4978
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5045..5839
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5089..5116)
                     /label=188-WKG-610-R
     polyA_signal    6013..6134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6179..6199)
                     /label=M13-rev
     primer_bind     complement(6183..6199)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(6205..6227)
                     /label=LacO
     protein_bind    6207..6223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6231..6261)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(6232..6261)
                     /label=lac
     protein_bind    6276..6297
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6403..7343
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(7344..8204)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8205..8309)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttcgcc accatgaaga tcatcctgtg gctgtgtgtg ttcggcctgt
      961 tcctggccac cctgttcccc atcagctggc agatgcccgt ggagtccggc ctgtcctccg
     1021 aggactccgc cagctccgag agcttcgcca agcgcatcaa gcgccacggc gagggcacct
     1081 tcaccagcga cgtgagcagc tacctggagg gccaggccgc caaggagttc atcgcctggc
     1141 tggtgaaggg ccgcggcata tcggccatgg ttagatctga caaaactcac acatgcccac
     1201 cgtgcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca
     1261 aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc
     1321 acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca
     1381 agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg
     1441 tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc aacaaagccc
     1501 tcccagcccc catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg
     1561 tgtacaccct gcccccatcc cgggaggaga tgaccaagaa ccaggtcagc ctgacctgcc
     1621 tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg
     1681 agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctaca
     1741 gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga
     1801 tgcacgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaag
     1861 ccagcggaag tggagcaacc aacttttccc tgctgaagca ggcaggcgac gtggaggaga
     1921 atcctggacc catgactagt gagacagaca cactcctgct atgggtactg ctgctctggg
     1981 ttccaggttc cactggtgac gctagtggtg gttctggtat ggtcttcaca ctcgaagatt
     2041 tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt gaacagggag
     2101 gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa aggattgtcc
     2161 tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat gaaggtctga
     2221 gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc
     2281 atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt acgccgaaca
     2341 tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca
     2401 ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg atcaaccccg
     2461 acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg ctgtgcgaac
     2521 gcattctggc gtgagcggcc gctcaatctc gagggatcca acttgaaccg gtaagaattg
     2581 caacaaacaa atcaccaccg tgaggatcac ccacggtggt gaaagcctta gcctagccgt
     2641 gaggatcacc cacggtaggc acaagaaatt ctacccgtga ggatcaccca cgggtagaat
     2701 cacaaagact acagccgtga ggatcaccca cggctgtaga ctcaaactta aaaaggatat
     2761 cttcagcaaa ctgatccaac ttgaaccggt aagaattgca acaaacaaat caccaccgtg
     2821 aggatcaccc acggtggtga aagccttagc ctagccgtga ggatcaccca cggtaggcac
     2881 aagaaattct acccgtgagg atcacccacg ggtagaatca caaagactac agccgtgagg
     2941 atcacccacg gctgtagact caaacttaaa aaggatatct tcagcaaact gatccaactt
     3001 gaaccggtaa gaattgcaac aaacaaatca ccaccgtgag gatcacccac ggtggtgaaa
     3061 gccttagcct agccgtgagg atcacccacg gtaggcacaa gaaattctac ccgtgaggat
     3121 cacccacggg tagaatcaca aagactacag ccgtgaggat cacccacggc tgtagactca
     3181 aacttaaaaa ggatatcttc agcaaactga tcggagacga gtccgtctca aattgcaaca
     3241 aacaaatcac caccgtgagg atcacccacg gtggtgaaag ccttagccta gccgtgagga
     3301 tcacccacgg taggcacaag aaattctacc cgtgaggatc acccacgggt agaatcacaa
     3361 agactacagc cgtgaggatc acccacggct gtagactcaa acttaaaaag gatatcttca
     3421 gcaaactgaa ttgcaacaaa caaatcacca ccgtgaggat cacccacggt ggtgaaagcc
     3481 ttagcctagc cgtgaggatc acccacggta ggcacaagaa attctacccg tgaggatcac
     3541 ccacgggtag aatcacaaag actacagccg tgaggatcac ccacggctgt agactcaaac
     3601 ttaaaaagga tatcttcagc aaactgaatt gcaacaaaca aatcaccacc gtgaggatca
     3661 cccacggtgg tgaaagcctt agcctagccg tgaggatcac ccacggtagg cacaagaaat
     3721 tctacccgtg aggatcaccc acgggtagaa tcacaaagac tacagccgtg aggatcaccc
     3781 acggctgtag actcaaactt aaaaaggata tcttcagcaa actgaattcg tttaaaccct
     3841 cctcgtccgg ggctgtgtcg ccccgctgac gagccctgaa aaagggcgaa acgaggagga
     3901 ctagtgggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     3961 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     4021 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     4081 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4141 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     4201 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4261 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4321 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4381 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4441 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4501 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4561 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4621 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4681 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4741 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4801 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4861 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     4921 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     4981 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     5041 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     5101 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     5161 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     5221 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     5281 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5341 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5401 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5461 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5521 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     5581 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5641 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5701 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5761 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5821 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     5881 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     5941 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6001 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6061 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6121 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6181 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6241 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6301 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6361 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6421 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6481 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6541 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6601 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6661 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6721 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6781 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6841 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6901 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6961 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7021 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7081 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7141 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7201 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7261 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7321 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7381 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7441 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7501 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7561 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7621 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7681 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7741 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7801 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7861 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7921 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7981 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8041 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8101 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8161 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8221 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8281 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//