| 质粒名称: | pLYL63 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000593 |
| 产品用途: | Mammalian VanR-specific 3xGNCR-NSP3 expression vector (VanO5-PhCMVmin-(GNCR1)3-NSP3-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | VanO5-PhCMVmin |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | (GNCR1)3-NSP3 |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLYL63
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLYL63载体质粒基本信息
pLYL63质粒图谱载体图谱和pLYL63载体序列质粒序列多克隆位点信息
pLYL63质粒载体简介
pLYL63质粒序列载体序列
LOCUS pLYL63 8246 bp DNA circular SYN 13-JAN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8246)
AUTHORS iGEM CPU-CHINA 2020
TITLE Direct Submission
JOURNAL Exported Jul 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8246
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 228
/label=E2F-1
misc_feature 232
/label=CMV
misc_feature 234..245
/label=VanO
/label=nonstandard type: operator
misc_feature 248..259
/label=VanO
/label=nonstandard type: operator
misc_feature 262..273
/label=VanO
/label=nonstandard type: operator
misc_feature 276..287
/label=VanO
/label=nonstandard type: operator
misc_feature 290..301
/label=VanO
/label=nonstandard type: operator
misc_feature 302..306
/label=E2F-1
misc_feature 307..455
/label=hcmvmin
primer_bind 436..456
/label=181-WKG-593-F
misc_feature 478..501
/label=New Feature
misc_feature 508
/label=CMV
primer_bind 552..571
/label=T7
CDS 598..1296
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
misc_feature 1313..1331
/label=linker
CDS 1333..2031
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
misc_feature 2048..2066
/label=linker
CDS 2068..2766
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
misc_feature 2783..2802
/label=linker
misc_feature 2803..3744
/label=NSP3
primer_bind complement(2814..2833)
/label=179-WKG-592-R
polyA_signal 3843..4067
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4113..4541
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4555..4884
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 4951..5745
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5919..6040
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6085..6105)
/label=M13-rev
misc_binding complement(6111..6133)
/label=LacO
promoter complement(6137..6167)
/label=lac promoter
/note="promoter for the E. coli lac operon"
misc_feature 6309..7249
/label=ColE1
/note="ColE1"
CDS complement(7250..8110)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(8111..8215)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtattggat
241 ccaatgcatt ggatccaatg gattggatcc aatcgattgg atccaattga ttggatccaa
301 tctagctcga gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag
361 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
421 ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattcgagc tcgcccgggg
481 atcctctaga gtcagcttct gcgagctctc tggctaacta gagaacccac tgcttactgg
541 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctaggc caccatggac
601 atcgagaagc tgtgcaagaa ggcggaagag gaggccaagg aggcccagga gaaggccgac
661 gagctgagac aacgacaccc cgattcgcag gcggcggaag acgcggagga tctcgcaaat
721 ctggctgtcg ccgccgtgct gaccgcttgt ctgctggccc aggagcatcc caacgccgat
781 atcgctaagc tgtgcatcaa ggccgcgagc gaagctgcgg aggccgcgtc aaaagccgcc
841 gagctggccc aacggcaccc agacagtcaa gcagccagag atgctatcaa actcgcctct
901 caagccgccc gcgccgtgat cctggccatc atgctcgccg cagagaatcc caacgctgat
961 attgcaaagc tatgtattaa ggcggccagc gaagccgcag aggctgcctc gaaagctgcg
1021 gaacttgcac agcggcaccc cgactcccag gccgcccgag atgcaattaa actggccagt
1081 caggccgctg aggcggtgga gagagccatc tggctggccg ccgagaaccc caatgccgat
1141 atcgccaaaa aatgcatcaa agcggcctcc gaagcagccg aggaggcctc taaagctgcc
1201 gaagaggctc aaagacaccc agacagccag aaggcacgcg acgagatcaa ggaggcctct
1261 cagaaggccg aggaggtgaa ggagagatgc aagagcgcca gcggaagtgg cggaggaggc
1321 gacgtcgcta gtgacatcga gaagctgtgc aagaaggcgg aagaggaggc caaggaggcc
1381 caggagaagg ccgacgagct gagacaacga caccccgatt cgcaggcggc ggaagacgcg
1441 gaggatctcg caaatctggc tgtcgccgcc gtgctgaccg cttgtctgct ggcccaggag
1501 catcccaacg ccgatatcgc taagctgtgc atcaaggccg cgagcgaagc tgcggaggcc
1561 gcgtcaaaag ccgccgagct ggcccaacgg cacccagaca gtcaagcagc cagagatgct
1621 atcaaactcg cctctcaagc cgcccgcgcc gtgatcctgg ccatcatgct cgccgcagag
1681 aatcccaacg ctgatattgc aaagctatgt attaaggcgg ccagcgaagc cgcagaggct
1741 gcctcgaaag ctgcggaact tgcacagcgg caccccgact cccaggccgc ccgagatgca
1801 attaaactgg ccagtcaggc cgctgaggcg gtggagagag ccatctggct ggccgccgag
1861 aaccccaatg ccgatatcgc caaaaaatgc atcaaagcgg cctccgaagc agccgaggag
1921 gcctctaaag ctgccgaaga ggctcaaaga cacccagaca gccagaaggc acgcgacgag
1981 atcaaggagg cctctcagaa ggccgaggag gtgaaggaga gatgcaagag cgccagcgga
2041 agtggcggag gaggcgacgt cgctagtgac atcgagaagc tgtgcaagaa ggcggaagag
2101 gaggccaagg aggcccagga gaaggccgac gagctgagac aacgacaccc cgattcgcag
2161 gcggcggaag acgcggagga tctcgcaaat ctggctgtcg ccgccgtgct gaccgcttgt
2221 ctgctggccc aggagcatcc caacgccgat atcgctaagc tgtgcatcaa ggccgcgagc
2281 gaagctgcgg aggccgcgtc aaaagccgcc gagctggccc aacggcaccc agacagtcaa
2341 gcagccagag atgctatcaa actcgcctct caagccgccc gcgccgtgat cctggccatc
2401 atgctcgccg cagagaatcc caacgctgat attgcaaagc tatgtattaa ggcggccagc
2461 gaagccgcag aggctgcctc gaaagctgcg gaacttgcac agcggcaccc cgactcccag
2521 gccgcccgag atgcaattaa actggccagt caggccgctg aggcggtgga gagagccatc
2581 tggctggccg ccgagaaccc caatgccgat atcgccaaaa aatgcatcaa agcggcctcc
2641 gaagcagccg aggaggcctc taaagctgcc gaagaggctc aaagacaccc agacagccag
2701 aaggcacgcg acgagatcaa ggaggcctct cagaaggccg aggaggtgaa ggagagatgc
2761 aagagcgcca gcggaagtgg cggaggaggc gacgtcgcta gcatgctcaa gatggagtct
2821 acacagcaga tggctagttc gattattaac acttcttttg aagccgcagt cgttgctgcc
2881 acttcaactc ttgaacttat gggcattcaa tatgattata atgaagttta tactagagtt
2941 aagagcaaat ttgattacgt aatggatgat tcaggtgtta aaaacaatct gttgggtaaa
3001 gcggcaacta ttgatcaggc actgaatgga aaatttggtt cagcagcaag aaatagaaat
3061 tggatggctg atactagaac tacagctaga ctagatgaag atgtgaacaa acttagaatg
3121 atgctatcat ctaaaggaat tgatcaaaaa atgagagtac ttaatgcatg tttcaatgtg
3181 aagcgagttc caggtaaatc atcatcaata attaaatgta ctagattaat gagagataag
3241 attgaacgtg gtgaagtcga agtggatgat tcgtttgttg aagaaaaaat ggaagttgac
3301 acaattgatt ggaaatctag atatgaacaa ttggaaaaac gatttgaatc gctgaaacaa
3361 agggtgaatg aaaaatatac aagttgggtg caaaaagcaa aaaaggtaaa tgaaaatatg
3421 tactctctcc aaaacgttat atcacaacag cagagccaga ttgctgattt acaaaattac
3481 tgtaataaat tggaagttga tctacaaaat aaaattagtt cacttgtgtc ttcagtcgaa
3541 tggtacctga aatcaatgga attgcctgat gaaattaaga cagatattga acaacaacta
3601 aattccattg atgtaatcaa tccaattaat gctatagatg attttgaatc actgatcaga
3661 aatattatac tcgactatga cagaatattt ttaatgttta aaggattaat gaggcaatgc
3721 aactatgaat atacgtatga ataggatcca ctagtccagt gtggtggaat tctgcagata
3781 tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
3841 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
3901 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
3961 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
4021 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
4081 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
4141 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
4201 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
4261 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
4321 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
4381 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
4441 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
4501 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
4561 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
4621 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
4681 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
4741 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
4801 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
4861 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
4921 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
4981 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
5041 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
5101 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
5161 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
5221 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
5281 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
5341 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
5401 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
5461 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
5521 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
5581 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
5641 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
5701 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
5761 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
5821 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
5881 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
5941 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
6001 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
6061 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
6121 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
6181 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
6241 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
6301 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
6361 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
6421 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
6481 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
6541 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
6601 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
6661 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
6721 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
6781 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
6841 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
6901 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
6961 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
7021 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
7081 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
7141 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
7201 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
7261 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
7321 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
7381 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
7441 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
7501 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
7561 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
7621 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
7681 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
7741 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
7801 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
7861 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
7921 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
7981 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
8041 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
8101 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
8161 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
8221 ttccccgaaa agtgccacct gacgtc
//
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