pAAV-AscI-CAG-dMito2-Nelle-L3-mCherry

LOCUS       pAAV-AscI-CAG-dMito2-Nelle-L3-mCherry               7534 bp DNA     circular SYN 15-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-AscI-CAG-dMito2-NeIle-L3-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Vax
  TITLE     Direct Submission
  JOURNAL   Exported Jul 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(941..1801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1932..2387)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    2661..2691
                     /label=AscI-OxE insert
                     /label=AscI-OxE\insert
                     /note="/vntifkey=21"
     misc_feature    2692..2935
                     /label=CMV enhancer
                     /label=CMV\enhancer
                     /note="/vntifkey=21"
     promoter        2942..3219
                     /label=chicken beta-actin promoter
     CDS             3646..3720
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3748..3819
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3847..5631
                     /codon_start=1
                     /label=NeIle
                     /note="/vntifkey=21"
                     /translation="MEDIKVAVVGAMSAGADWRISKEACPNGKTLISTLKWSYTTGDGK
                     RYRSTARTTFTFAKPMAANYLKNQPMYVFRRTELKHSKTEMDFKEWQKAFTDVVGMDEL
                     YKVDGGSGGTGMVSEGEVDNMASLPATHELHIFGSINGVGFDMVGQGSGNPNVGYEELN
                     LKSTKGELQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTVRFEDGAS
                     LTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAFHIGPVTQYGDQEFTGAEQAV
                     ADINARGGIKGNKLQIVKYDDACDPKQAVAVANRVVNDGIKYVIGHLCSSSAQPASDIY
                     EDAGILMITPAATAPELTARGYQLILRTTGLDSDQGLTAAKYILEKVKPQRIAIVHDKQ
                     QYGEGLARAVQGGLKKGNANVVFFDGITAGEKDFSTLVARLKKENIDFVYYGGYHPEMG
                     QILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYDQVPANKHIVDAVK
                     AKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDTVMGPLTWDEKGDLK
                     GFEFGVFDWHANGTATDAK"
     CDS             5695..6405
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    6436..7024
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     polyA_signal    7067..7188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(7534,1..940)
                     /label=ColE1
                     /note="ColE1"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
     2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
     3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
     3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
     3241 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     3301 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     3361 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     3421 ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct gccttcgggg
     3481 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     3541 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     3601 gctgtctcat cattttggca aagaattgga tccaccggtg acaccatgtc cgtcctgacg
     3661 ccgctgctgc tgcggggctt gacaggctcg gcccggcggc tcccagtgcc gcgcgccaag
     3721 atccattcgt tggggcctgc aagatccagt gtattaacac ccctgctgct gcggggcttg
     3781 acaggctcgg cccggcggct cccagtgccg cgcgccaaga ttcacagtct tggtccagct
     3841 agatctatgg aagatattaa agtcgcggtc gtgggcgcaa tgtccgctgg tgctgactgg
     3901 cgtatatcca aggaggcttg ccccaatggt aagactctta tttccacact taagtggtct
     3961 tataccaccg gcgacggcaa acgctaccgg tctactgctc gcaccacatt cacattcgcc
     4021 aaaccaatgg ccgcaaatta cctcaaaaat cagccgatgt atgtcttccg gagaacagag
     4081 ctgaaacatt ctaagaccga aatggatttt aaagagtggc aaaaggcctt tacggacgtg
     4141 gtgggcatgg acgagctgta caaggtggat ggcggcagcg gtggcaccgg catggtctca
     4201 gagggagagg tggacaacat ggctagtctg cctgctaccc acgaactgca tatttttggc
     4261 tccataaatg gcgtcggttt cgacatggta ggccaaggct ccggtaatcc aaacgtcggt
     4321 tatgaggagc tgaacctgaa gtccaccaag ggcgaactgc agttcagtcc ctggatcctg
     4381 gtgccgcaca ttgggtatgg gtttcatcag tatcttccct acccggacgg gatgtcgcca
     4441 tttcaggctg caatggtcga cgggtccgga tatcaggtgc accgcactgt gcggtttgag
     4501 gacggtgctt cactgaccgt gaattacaga tacacctacg aaggatcaca tatcaaaggg
     4561 gaagctcagg tgaagggcac tgggttcccg gcagacggcc ctgtgatgac aaattccctg
     4621 acagcctttc atattggtcc ggtgacccag tacggtgacc aggagtttac cggcgcagag
     4681 caggcggttg cggatatcaa cgctagaggc ggcattaagg gcaacaaact gcaaatcgta
     4741 aaatatgacg atgcctgtga cccgaaacag gctgttgcgg tggcgaacag agtcgttaac
     4801 gacggcatta aatatgtgat tggtcacctc tgttcttcat cagcgcagcc tgcgtctgac
     4861 atctacgaag acgcaggcat tttaatgatc accccagcgg caaccgcgcc ggagctgacc
     4921 gcccgtggct atcagctgat cctgcgcacc accggcctgg actccgacca ggggctgacg
     4981 gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta ttgctatcgt tcacgacaaa
     5041 cagcagtacg gcgaaggtct ggcgcgagcg gtgcagggtg gcctgaagaa aggcaatgca
     5101 aacgtggtgt tctttgatgg catcaccgcc ggggaaaaag atttctcaac gctggtggcg
     5161 cgtctgaaaa aagagaatat cgacttcgtt tactacggcg gttatcaccc ggaaatgggg
     5221 caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc agtttatggg gccggagggt
     5281 gtggctaacg tttcgctgtc taacattgcg ggcgaatcag cggaagggct gctggtgacc
     5341 aagccgaaga actacgatca ggttccggcg aacaaacaca ttgttgacgc agtcaaagcg
     5401 aaaaaacagg acccaagtgg cgcattcgtt tggaccacct acgccgcgct gcaatctttg
     5461 caggcgggcc tgaatcagtc tgacgatccg gctgaaatcg ccaaatacct gaaagcgaac
     5521 tccgtggata ccgtaatggg accgctgacc tgggatgaga aaggcgatct gaaaggcttt
     5581 gagttcggcg tatttgactg gcacgccaac ggcacggcga ccgatgcgaa ggaattcggt
     5641 ggttctggtt ctggagggag tggcagcaag cttggtggtt ctggttctgg taccatggtg
     5701 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     5761 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     5821 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     5881 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     5941 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     6001 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     6061 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     6121 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     6181 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     6241 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     6301 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     6361 gagggccgcc actccaccgg cggcatggac gagctgtata agtaaactag tgaattcgat
     6421 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6481 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6541 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6601 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6661 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6721 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6781 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6841 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6901 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6961 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7021 ccgcatcgat accgtcgacc cgggcggccg cttcgagcag acatgataag atacattgat
     7081 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     7141 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     7201 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
     7261 aacctctaca aatgtggtaa aatcgataag gatcttccta gagcatggct acgtagataa
     7321 gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc
     7381 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     7441 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcattaatg aatcggccaa
     7501 cgcgcgggga gaggcggttt gcgtattggg cgct
//