pLZ527

LOCUS       pLZ527              8681 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    895..900
                     /label=NheI
     misc_feature    911..915
                     /label=New Feature
     misc_feature    911..915
                     /label=New Feature
     CDS             926..2428
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
                     AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
                     HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
                     TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
                     YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
                     LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
                     AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
                     RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
                     RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     polyA_signal    2477..2485
                     /label=SV40 late polyA
     CDS             2486..2542
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2543..2872
                     /label=mINS
     primer_bind     2832..2852
                     /label=202-WKG-632-F
     misc_feature    2901..2904
                     /label=linker
     misc_feature    2921..2933
                     /label=linker
     misc_feature    2939..2957
                     /label=MS2 box
     misc_feature    2963..2972
                     /label=linker
     misc_feature    2978..2996
                     /label=MS2 box
     misc_feature    3002..3011
                     /label=linker
     misc_feature    3002..3011
                     /label=linker
     misc_feature    3010..3040
                     /label=hairpin
     misc_feature    3016..3034
                     /label=MS2 box
     misc_feature    3039..3051
                     /label=linker
     misc_feature    3050..3080
                     /label=hairpin
     misc_feature    3056..3074
                     /label=MS2 box
     misc_feature    3084..3089
                     /label=linker
     misc_feature    3098..3113
                     /label=linker
     misc_feature    3119..3122
                     /label=linker
     misc_feature    3139..3151
                     /label=linker
     misc_feature    3157..3175
                     /label=MS2 box
     misc_feature    3181..3190
                     /label=linker
     misc_feature    3196..3214
                     /label=MS2 box
     misc_feature    3220..3229
                     /label=linker
     misc_feature    3220..3229
                     /label=linker
     misc_feature    3228..3258
                     /label=hairpin
     misc_feature    3234..3252
                     /label=MS2 box
     misc_feature    3257..3269
                     /label=linker
     misc_feature    3268..3298
                     /label=hairpin
     misc_feature    3274..3292
                     /label=MS2 box
     misc_feature    3302..3307
                     /label=linker
     misc_feature    3316..3331
                     /label=linker
     misc_feature    3337..3340
                     /label=linker
     misc_feature    3357..3369
                     /label=linker
     misc_feature    3375..3393
                     /label=MS2 box
     misc_feature    3399..3408
                     /label=linker
     misc_feature    3414..3432
                     /label=MS2 box
     misc_feature    3438..3447
                     /label=linker
     misc_feature    3438..3447
                     /label=linker
     misc_feature    3446..3476
                     /label=hairpin
     misc_feature    3452..3470
                     /label=MS2 box
     misc_feature    3475..3487
                     /label=linker
     misc_feature    3486..3516
                     /label=hairpin
     misc_feature    3492..3510
                     /label=MS2 box
     misc_feature    3520..3525
                     /label=linker
     misc_feature    3534..3549
                     /label=linker
     misc_feature    3576..3588
                     /label=linker
     misc_feature    3594..3612
                     /label=MS2 box
     misc_feature    3618..3627
                     /label=linker
     misc_feature    3633..3651
                     /label=MS2 box
     misc_feature    3657..3666
                     /label=linker
     misc_feature    3657..3666
                     /label=linker
     misc_feature    3665..3695
                     /label=hairpin
     misc_feature    3671..3689
                     /label=MS2 box
     misc_feature    3694..3706
                     /label=linker
     misc_feature    3705..3735
                     /label=hairpin
     misc_feature    3711..3729
                     /label=MS2 box
     misc_feature    3739..3744
                     /label=linker
     misc_feature    3753..3768
                     /label=linker
     misc_feature    3774..3786
                     /label=linker
     misc_feature    3792..3810
                     /label=MS2 box
     misc_feature    3816..3825
                     /label=linker
     misc_feature    3831..3849
                     /label=MS2 box
     misc_feature    3855..3864
                     /label=linker
     misc_feature    3855..3864
                     /label=linker
     misc_feature    3863..3893
                     /label=hairpin
     misc_feature    3869..3887
                     /label=MS2 box
     misc_feature    3892..3904
                     /label=linker
     misc_feature    3903..3933
                     /label=hairpin
     misc_feature    3909..3927
                     /label=MS2 box
     misc_feature    3937..3942
                     /label=linker
     misc_feature    3951..3966
                     /label=linker
     misc_feature    3972..3984
                     /label=linker
     misc_feature    3990..4008
                     /label=MS2 box
     misc_feature    4014..4023
                     /label=linker
     misc_feature    4029..4047
                     /label=MS2 box
     misc_feature    4053..4062
                     /label=linker
     misc_feature    4053..4062
                     /label=linker
     misc_feature    4061..4091
                     /label=hairpin
     misc_feature    4067..4085
                     /label=MS2 box
     misc_feature    4090..4102
                     /label=linker
     misc_feature    4101..4131
                     /label=hairpin
     misc_feature    4107..4125
                     /label=MS2 box
     misc_feature    4135..4140
                     /label=linker
     misc_feature    4149..4164
                     /label=linker
     misc_feature    4171..4246
                     /label=HHR Env140_nutR_H6
     misc_feature    complement(4179..4187)
                     /label=Stem III
     misc_feature    4190..4205
                     /label=Stem I
     terminator      4214..4228
                     /label=nutR-boxB
     misc_feature    4232..4240
                     /label=Stem III
     polyA_signal    4278..4502
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4548..4976
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4562..4586)
                     /label=184-WKG-599-R
     primer_bind     4847..4866
                     /label=189-WKG-612-F
     promoter        4990..5319
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5386..6180
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6354..6475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6520..6540)
                     /label=M13-rev
     primer_bind     complement(6524..6540)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(6546..6568)
                     /label=LacO
     protein_bind    6548..6564
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6572..6602)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(6573..6602)
                     /label=lac
     protein_bind    6617..6638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6744..7684
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(7685..8545)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8546..8650)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttagcg ccaccatgct gctgctgctg ctgctgctgg gcctgaggct
      961 acagctctcc ctgggcatca tcccagttga ggaggagaac ccggacttct ggaaccgcga
     1021 ggcagccgag gccctgggtg ccgccaagaa gctgcagcct gcacagacag ccgccaagaa
     1081 cctcatcatc ttcctgggcg atgggatggg ggtgtctacg gtgacagctg ccaggatcct
     1141 aaaagggcag aagaaggaca aactggggcc tgagataccc ctggccatgg accgcttccc
     1201 atatgtggct ctgtccaaga catacaatgt agacaaacat gtgccagaca gtggagccac
     1261 agccacggcc tacctgtgcg gggtcaaggg caacttccag accattggct tgagtgcagc
     1321 cgcccgcttt aaccagtgca acacgacacg cggcaacgag gtcatctccg tgatgaatcg
     1381 ggccaagaaa gcagggaagt cagtgggagt ggtaaccacc acacgagtgc agcacgcctc
     1441 gccagccggc acctacgccc acacggtgaa ccgcaactgg tactcggacg ccgacgtgcc
     1501 tgcctcggcc cgccaggagg ggtgccagga catcgctacg cagctcatct ccaacatgga
     1561 cattgacgtg atcctaggtg gaggccgaaa gtacatgttt cgcatgggaa ccccagaccc
     1621 tgagtaccca gatgactaca gccaaggtgg gaccaggctg gacgggaaga atctggtgca
     1681 ggaatggctg gcgaagcgcc agggtgcccg gtatgtgtgg aaccgcactg agctcatgca
     1741 ggcttccctg gacccgtctg tgacccatct catgggtctc tttgagcctg gagacatgaa
     1801 atacgagatc caccgagact ccacactgga cccctccctg atggagatga cagaggctgc
     1861 cctgcgcctg ctgagcagga acccccgcgg cttcttcctc ttcgtggagg gtggtcgcat
     1921 cgaccatggt catcatgaaa gcagggctta ccgggcactg actgagacga tcatgttcga
     1981 cgacgccatt gagagggcgg gccagctcac cagcgaggag gacacgctga gcctcgtcac
     2041 tgccgaccac tcccacgtct tctccttcgg aggctacccc ctgcgaggga gctccatctt
     2101 cgggctggcc cctggcaagg cccgggacag gaaggcctac acggtcctcc tatacggaaa
     2161 cggtccaggc tatgtgctca aggacggcgc ccggccggat gttaccgaga gcgagagcgg
     2221 gagccccgag tatcggcagc agtcagcagt gcccctggac gaagagaccc acgcaggcga
     2281 ggacgtggcg gtgttcgcgc gcggcccgca ggcgcacctg gttcacggcg tgcaggagca
     2341 gaccttcata gcgcacgtca tggccttcgc cgcctgcctg gagccctaca ccgcctgcga
     2401 cctggcgccc cccgccggca ccaccgacgc cgcgcacccg ggttactcta gagtcggggc
     2461 ggccggccgc ttcgagcaga caggagcaac caacttttcc ctgctgaagc aggcaggcga
     2521 cgtggaggag aatcctggac ccatggccct gtggatgcgc ttcctgcccc tgctggccct
     2581 gctcgtcctc tgggagccca agcctgccca ggcttttgtc aaacagcacc tttgtggtcc
     2641 tcacctggtg gaggctctgt acctggtgtg tggggaacgt ggtttcttct acacacccaa
     2701 gtcccgtcgt aaaagggagg acccgcaagt gccacaactg gagctgggtg gaggcccgga
     2761 ggccggggat cttcagacct tggcactgga ggttgcccgg cagaagcgtg gcattgtgga
     2821 tcagtgctgc accagcatct gctccctcta ccaactggag aactactgca actgagcggc
     2881 cgctcaatct cgagggatcc aacttgaacc ggtaagaatt gcaacaaaca aatcaccacc
     2941 gtgaggatca cccacggtgg tgaaagcctt agcctagccg tgaggatcac ccacggtagg
     3001 cacaagaaat tctacccgtg aggatcaccc acgggtagaa tcacaaagac tacagccgtg
     3061 aggatcaccc acggctgtag actcaaactt aaaaaggata tcttcagcaa actgatccaa
     3121 cttgaaccgg taagaattgc aacaaacaaa tcaccaccgt gaggatcacc cacggtggtg
     3181 aaagccttag cctagccgtg aggatcaccc acggtaggca caagaaattc tacccgtgag
     3241 gatcacccac gggtagaatc acaaagacta cagccgtgag gatcacccac ggctgtagac
     3301 tcaaacttaa aaaggatatc ttcagcaaac tgatccaact tgaaccggta agaattgcaa
     3361 caaacaaatc accaccgtga ggatcaccca cggtggtgaa agccttagcc tagccgtgag
     3421 gatcacccac ggtaggcaca agaaattcta cccgtgagga tcacccacgg gtagaatcac
     3481 aaagactaca gccgtgagga tcacccacgg ctgtagactc aaacttaaaa aggatatctt
     3541 cagcaaactg atcggagacg agtccgtctc aaattgcaac aaacaaatca ccaccgtgag
     3601 gatcacccac ggtggtgaaa gccttagcct agccgtgagg atcacccacg gtaggcacaa
     3661 gaaattctac ccgtgaggat cacccacggg tagaatcaca aagactacag ccgtgaggat
     3721 cacccacggc tgtagactca aacttaaaaa ggatatcttc agcaaactga attgcaacaa
     3781 acaaatcacc accgtgagga tcacccacgg tggtgaaagc cttagcctag ccgtgaggat
     3841 cacccacggt aggcacaaga aattctaccc gtgaggatca cccacgggta gaatcacaaa
     3901 gactacagcc gtgaggatca cccacggctg tagactcaaa cttaaaaagg atatcttcag
     3961 caaactgaat tgcaacaaac aaatcaccac cgtgaggatc acccacggtg gtgaaagcct
     4021 tagcctagcc gtgaggatca cccacggtag gcacaagaaa ttctacccgt gaggatcacc
     4081 cacgggtaga atcacaaaga ctacagccgt gaggatcacc cacggctgta gactcaaact
     4141 taaaaaggat atcttcagca aactgaattc gtttaaaccc tcctcgtccg gggctgtgtc
     4201 gccccgctga cgagccctga aaaagggcga aacgaggagg actagtgggc ccgtttaaac
     4261 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     4321 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4381 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4441 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4501 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     4561 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4621 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4681 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4741 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4801 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4861 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4921 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     4981 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     5041 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     5101 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     5161 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5221 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     5281 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     5341 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     5401 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     5461 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     5521 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     5581 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     5641 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5701 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     5761 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     5821 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5881 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     5941 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6001 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6061 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6121 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     6181 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     6241 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     6301 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     6361 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6421 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6481 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6541 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6601 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6661 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6721 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6781 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6841 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6901 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6961 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7021 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7081 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7141 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7201 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7261 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7321 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7381 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7441 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7501 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7561 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7621 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7681 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7741 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7801 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7861 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7921 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7981 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8041 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8101 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8161 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8221 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8281 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8341 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8401 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8461 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8521 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8581 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8641 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//