pLZ285

LOCUS       pLZ285                7209 bp DNA     circular SYN 10-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    191..198
                     /label=CRE (RIP)
     misc_feature    206..213
                     /label=CRE (RIP)
     misc_feature    221..228
                     /label=CRE (RIP)
     misc_feature    236..243
                     /label=CRE (RIP)
     promoter        274..305
                     /label=P_min
     regulatory      340..349
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    346..408
                     /label=Igk secretion signal
     CDS             414
                     /codon_start=1
                     /label=linker
                     /translation=""
     CDS             415..1113
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     misc_feature    1120..1863
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     misc_feature    1393..1395
                     /label=F93A
                     /note="F93A"
     misc_feature    1847..1863
                     /label=EpoR-D1_D2_TM
     CDS             1873..2701
                     /codon_start=1
                     /label=IL6ST
                     /translation="NKRDLIKKHIWPNVPDPSKSHIAQWSPHTPPRHNFNSKDQMYSDG
                     NFTDVSVVEIEANDKKPFPEDLKSLDLFKKEKINTEGHSSGIGGSSCMSSSRPSISSSD
                     ENESSQNTSSTVQASTVVHSGYRHQVPSVQVFSRSESTQPLLDSEERPEDLQLVDHVDG
                     GDGIFPRQQYFKQNCSQHESSPDISHFERSKQVSSVNEEDFVRLKQQISDHISQSCGSG
                     QMKMFQEVSAADAFGPGTEGQVERFETVGMEAATDEGMPKSYLPQTVRQGGYMP"
     misc_feature    1900..1926
                     /label=box1
     misc_feature    2224..2226
                     /label=Y759A
     polyA_signal    2806..3030
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3076..3504
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3518..3847
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3914..4708
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4882..5003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5048..5068)
                     /label=M13-rev
     primer_bind     complement(5052..5068)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(5074..5096)
                     /label=LacO
     protein_bind    5076..5092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5100..5130)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(5101..5130)
                     /label=lac
     protein_bind    5145..5166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5272..6212
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(6213..7073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7074..7178)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(7115..7143)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattggagc tcttacgcgt
      181 gctagcagcc tgacgtccga gagcctgacg tccgagagcc tgacgtccga gagcctgacg
      241 tccgagatct ctcgaggtcg acagcggaga ctctagaggg tatataatgg aagctcgact
      301 tccagcttgg caatccggta ctgttggtaa agaaagcttg ccaccatgga gaccgatacc
      361 ttattactgt gggtgcttct tctgtgggtt cccggtagca ccggagacgg atccgacatc
      421 gagaagctgt gcaagaaggc ggaagaggag gccaaggagg cccaggagaa ggccgacgag
      481 ctgagacaac gacaccccga ttcgcaggcg gcggaagacg cggaggatct cgcaaatctg
      541 gctgtcgccg ccgtgctgac cgcttgtctg ctggcccagg agcatcccaa cgccgatatc
      601 gctaagctgt gcatcaaggc cgcgagcgaa gctgcggagg ccgcgtcaaa agccgccgag
      661 ctggcccaac ggcacccaga cagtcaagca gccagagatg ctatcaaact cgcctctcaa
      721 gccgcccgcg ccgtgatcct ggccatcatg ctcgccgcag agaatcccaa cgctgatatt
      781 gcaaagctat gtattaaggc ggccagcgaa gccgcagagg ctgcctcgaa agctgcggaa
      841 cttgcacagc ggcaccccga ctcccaggcc gcccgagatg caattaaact ggccagtcag
      901 gccgctgagg cggtggagag agccatctgg ctggccgccg agaaccccaa tgccgatatc
      961 gccaaaaaat gcatcaaagc ggcctccgaa gcagccgagg aggcctctaa agctgccgaa
     1021 gaggctcaaa gacacccaga cagccagaag gcacgcgacg agatcaagga ggcctctcag
     1081 aaggccgagg aggtgaagga gagatgcaag agcgaattcg caccttcacc cagcctcccg
     1141 gaccccaagt ttgagagcaa agcggccctg ctggcatccc ggggctccga agaacttctg
     1201 tgcttcaccc aacgcttgga agacttggtg tgtttctggg aggaagcggc gagctccggg
     1261 atggacttca actacagctt ctcataccag ctcgagggtg agtcacgaaa gtcatgtagc
     1321 ctgcaccagg ctcccaccgt ccgcggctcc gtgcgtttct ggtgttcact gccaacagcg
     1381 gacacatcga gtgctgtgcc gctggagctg caggtgacgg aggcgtccgg ttctcctcgc
     1441 tatcaccgca tcatccatat caatgaagta gtgctcctgg acgcccccgc ggggctgctg
     1501 gcgcgccggg cagaagaggg cagccacgtg gtgctgcgct ggctgccacc tcctggagca
     1561 cctatgacca cccacatccg atatgaagtg gacgtgtcgg caggcaaccg ggcaggaggg
     1621 acacaaaggg tggaggtcct ggaaggccgc actgagtgtg ttctgagcaa cctgcggggc
     1681 gggacgcgct acaccttcgc tgttcgagcg cgcatggccg agccgagctt cagcggattc
     1741 tggagtgcct ggtctgagcc cgcgtcacta ctgaccgcta gcgacctgga ccctctcatc
     1801 ttgacgctgt ctctcattct ggtcctcatc tcgttgttgc tgacggttct ggccctgctg
     1861 tccgcagcag caaataagcg agacctaatt aaaaaacaca tctggcctaa tgttccagat
     1921 ccttcaaaga gtcatattgc ccagtggtca cctcacactc ctccaaggca caattttaat
     1981 tcaaaagatc aaatgtattc agatggcaat ttcactgatg taagtgttgt ggaaatagaa
     2041 gcaaatgaca aaaagccttt tccagaagat ctgaaatcat tggacctgtt caaaaaggaa
     2101 aaaattaata ctgaaggaca cagcagtggt attggggggt cttcatgcat gtcatcttct
     2161 aggccaagca tttctagcag tgatgaaaat gaatcttcac aaaacacttc gagcactgtc
     2221 caggcttcta ccgtggtaca cagtggctac agacaccaag ttccgtcagt ccaagtcttc
     2281 tcaagatccg agtctaccca gcccttgtta gattcagagg agcggccaga agatctacaa
     2341 ttagtagatc atgtagatgg cggtgatggt atttttccca ggcaacagta cttcaaacag
     2401 aactgcagtc agcatgaatc cagtccagat atttcacatt ttgaaaggtc aaagcaagtt
     2461 tcatcagtca atgaggaaga ttttgttaga cttaaacagc agatttcaga tcatatttca
     2521 caatcctgtg gatctgggca aatgaaaatg tttcaggaag tttctgcagc agatgctttt
     2581 ggtccaggta ctgagggaca agtagaaaga tttgaaacag ttggcatgga ggctgcgact
     2641 gatgaaggca tgcctaaaag ttacttacca cagactgtac ggcaaggcgg ctacatgcct
     2701 cagtaaggat ccactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc
     2761 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     2821 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     2881 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     2941 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3001 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     3061 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3121 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3181 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3241 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     3301 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3361 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     3421 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3481 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     3541 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3601 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3661 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3721 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3781 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3841 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     3901 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3961 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4021 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4081 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4141 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4201 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4261 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4321 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4381 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4441 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4501 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4561 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4621 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4681 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     4741 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4801 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     4861 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     4921 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4981 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     5041 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5101 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5161 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5221 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5281 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5341 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5401 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5461 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5521 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5581 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5641 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5701 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5761 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5821 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5881 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5941 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6001 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6061 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7201 cctgacgtc
//