pGL4.70[hRluc]

价格:1500元

联系方式:I47-825O-882O

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pGL4.70载体基本信息

出品公司: Promega
载体名称: pGL4.70 ; pGL4.70[hRluc]
质粒类型: 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 3522 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: hRluc
载体抗性: 氨苄青霉素
筛选标记: 潮霉素(Hygromycin)
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.70[hRluc]载体是信号通路报告载体;无启动子;含海肾 荧光素酶报告基因hRluc。
产品目录号: E6881
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.70载体质粒图谱和多克隆位点信息

pGL4.70载体图谱



pGL4.70 载体特征

pGL4.70载体简介

The pGL4.70[hRluc] Vector encodes the hRluc gene (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.70[hRluc] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows for cloning of a promoter of choice.

pGL4.70载体序列

LOCUS       AY738226                3522 bp    DNA     circular SYN 08-NOV-2004
DEFINITION  Renilla luciferase reporter vector pGL4.70[hRluc], complete
            sequence.
ACCESSION   AY738226
VERSION     AY738226.1  GI:55535630
KEYWORDS    .
SOURCE      Renilla luciferase reporter vector pGL4.70[hRluc]
  ORGANISM  Renilla luciferase reporter vector pGL4.70[hRluc]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Almond,B., Chauvin,F. and Browning,J.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
            Corporation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Almond,B., Chauvin,F. and Browning,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2004) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Renilla luciferase reporter vector
                     pGL4.70[hRluc]"
                     /mol_type="other DNA"
                     /db_xref="taxon:296663"
     misc_feature    1..70
                     /note="multiple cloning site region"
     CDS             100..1035
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase hRluc2"
                     /protein_id="AAV52877.1"
                     /db_xref="GI:55535631"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1067..1288
                     /note="SV40 late poly(A) region"
     primer_bind     1356..1375
                     /note="reporter vector primer 4 (RVPrimer4)"
     rep_origin      1613
                     /note="ColE1-derived"
     gene            complement(2404..3264)
                     /gene="ampR"
     CDS             complement(2404..3264)
                     /gene="ampR"
                     /note="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="AAV52878.1"
                     /db_xref="GI:55535632"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      3369..3522
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic; transcriptional pause site"
     primer_bind     complement(3471..3490)
                     /note="reporter vector primer 3 (RVPrimer3)"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     1081 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1141 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1201 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     1261 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt
     1321 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     1381 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
     1441 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1501 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1561 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1621 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1681 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1741 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1801 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1861 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1921 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1981 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2041 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2101 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2161 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2221 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2281 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2341 tctaaagtat atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag
     2401 tggttaccag tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc
     2461 catagtggcc tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg
     2521 ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat
     2581 gaaccagcca gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat
     2641 ccagtctatg agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg
     2701 aagagttgtg gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc
     2761 gttcaactct ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa
     2821 tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc
     2881 gctcatggta atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt
     2941 ttccgtgacc ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag
     3001 ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt
     3061 gctcatcatc gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag
     3121 atccagttcg atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac
     3181 cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc
     3241 gacacgaaaa tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca
     3301 gggttactag tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga
     3361 caggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     3421 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
     3481 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ct
//

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