pGL3 basic

价格:800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Promega
载体名称: pGL3 basic, pGL3basic, pGL3-Basic
质粒类型: 荧光素酶报告载体
高拷贝/低拷贝: --
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4818bp
5' 测序引物及序列: RVprimer3: CTAGCAAAATAGGCTGTCCC
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
筛选标记: --
备注: --
产品目录号: E1751
稳定性: --
组成型: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pGL3 basic载体图谱

载体简介

载体序列

LOCUS       pGL3-Basic	4818 bp 	DNA   circular	 SYN
DEFINITION  pGL3-Basic
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="pGL3-Basic"
                     /mol_type="other DNA"
     gene            88..1740
                     /label="firefly_Luciferase"
                     /gene="firefly_Luciferase"
     CDS             88..1740
                     /label="ORF frame 1"
     misc_feature    complement(154..172)
                     /label="LucNrev_primer"
     misc_feature    complement(1792..1811)
                     /label="EBV_rev_primer"
     rep_origin      complement(2306..2925)
                     /label="pBR322_origin"
     gene            complement(3080..3940)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(3080..3940)
                     /label="ORF frame 3"
     promoter        complement(3982..4010)
                     /label="AmpR_promoter"
     rep_origin      4089..4395
                     /label="f1_origin"
     misc_feature    4658..4811
                     /label="TB"
     terminator      4658..4706
                     /label="synth_PA_terminator"
     terminator      4658..4811
                     /label="up_PA_terminator"
     misc_feature    4760..4779
                     /label="RVprimer3_primer"
ORIGIN
    1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg
   61 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg
  121 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag
  181 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc
  241 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
  301 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
  361 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
  421 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag
  481 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc
  541 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
  601 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca
  661 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct
  721 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc
  781 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
  841 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
  901 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta
  961 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
 1021 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc
 1081 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt
 1141 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
 1201 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt
 1261 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
 1321 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
 1381 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc
 1441 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt
 1501 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
 1561 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
 1621 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
 1681 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa
 1741 ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt
 1801 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
 1861 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
 1921 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
 1981 tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag ccttcaaccc
 2041 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
 2101 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg
 2161 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
 2221 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
 2281 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
 2341 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
 2401 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
 2461 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
 2521 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
 2581 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
 2641 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
 2701 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
 2761 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
 2821 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
 2881 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
 2941 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
 3001 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
 3061 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
 3121 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
 3181 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
 3241 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
 3301 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
 3361 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
 3421 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
 3481 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
 3541 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
 3601 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
 3661 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
 3721 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
 3781 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
 3841 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
 3901 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
 3961 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
 4021 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
 4081 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
 4141 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
 4201 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
 4261 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
 4321 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
 4381 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
 4441 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
 4501 ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc aactgttggg
 4561 aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa
 4621 gtaatattaa ggtacgggag gtacttggag cggccgcaat aaaatatctt tattttcatt
 4681 acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa
 4741 caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag
 4801 aacatttctc tatcgata
//

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