pBI-CMV2

价格:2200元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pBI-CMV2
质粒类型: 哺乳细胞表达载体 ; 双向表达载体;荧光报告载体
高拷贝/低拷贝: 低拷贝
启动子: 弱启动子minCMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3822 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillin )
筛选标记: --
备注: pBI-CMV2表达绿色荧光蛋白AcGFP1
产品目录号: #631631
稳定性: --
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pBI-CMV2载体图谱



pBI-CMV2多克隆位点

载体简介

双向启动子载体pBI-CMV2表达绿色荧光蛋白AcGFP1 
The pBI bidirectional vectors are designed to constitutively express a protein of interest and a reporter protein (or two proteins of interest). Expression is driven by constitutively active bidirectional human cytomegalovirus promoters (PminCMV). The gene(s) of interest must contain an initiation codon and a stop codon. 
eatures Constitutively express a protein of interest and a reporter protein (or two proteins of interest) 
pBI-CMV2 allows you to express a protein of interest and AcGFP1, a monomeric green fluorescent protein derived from Aequorea coerulescens.

载体序列

LOCUS       pBI-CMV2	3822 bp 	DNA	SYN
DEFINITION  pBI-CMV2
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="pBI-CMV2"
                     /mol_type="other DNA"
     promoter        4..568
                     /label="CMV_immearly_promoter"
     misc_feature    71..358
                     /label="CAG_enhancer"
     misc_feature    525..545
                     /label="CMV_fwd_primer"
     promoter        526..595
                     /label="CMV_promoter"
     gene            complement(1799..2659)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(1799..2659)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(2701..2729)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             2969..3520
                     /label="ORF frame 2"
                     /translation="MADYDPLDCSLRRSGPSLVQLIHAVGDGGGGHEAEVDHVIALLV
                     GVLGQGALGGQVVVIGQQHRAIADGGILLVVVGQLHAAILDVVADLEVHLDAILGLVG
                     HDVHIVGVVVVLHLIAQDVAILLEIGAGQLDAIHQGIALELHLGARLVVAVILEEDGA
                     LLDVALRHGALEEVVLLHVIGVA*"
     CDS             complement(3017..3736)
                     /label="ORF frame 3"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                     DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MIYFGFVTAAAITHGMDELYK*"
     gene            complement(3020..3733)
                     /label="AcGFP1"
                     /gene="AcGFP1"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                     DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MIYFGFVTAAAITHGMDELYK*"
     misc_feature    complement(3756..3780)
                     /label="LNCX_primer"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                     DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MIYFGFVTAAAITHGMDELYK*"
     misc_feature    complement(3763..3782)
                     /label="pCEP_fwd_primer"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                     DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MIYFGFVTAAAITHGMDELYK*"
ORIGIN
    1 ctcgagtagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
   61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
  121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
  181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
  241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
  301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
  361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
  421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
  481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
  541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
  601 gggatcctct agtcagctga cgcgtgctag cgcggccgca tcgataagct tgtcgacgat
  661 atctccagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
  721 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
  781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
  841 aagcattttt ttcactgccc cgagcttcct cgctcactga ctcgctgcgc tcggtcgttc
  901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
  961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
 1021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
 1081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
 1141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
 1201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
 1261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
 1321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
 1381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
 1441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
 1501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
 1561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
 1621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
 1681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
 1741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
 1801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
 1861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
 1921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
 1981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
 2041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
 2101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
 2161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
 2221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
 2281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
 2341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
 2401 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
 2461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
 2521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
 2581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
 2641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
 2701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
 2761 cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag tgaaaaaaat gctttatttg
 2821 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
 2881 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
 2941 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag actgcagcct
 3001 caggagatct gggccctcac ttgtacagct catccatgcc gtgggtgatg gcggcggcgg
 3061 tcacgaagcc gaagtagatc atgtgatcgc gcttctcgtt ggggtccttg gacagggcgc
 3121 tctgggtgga caggtagtgg ttatcgggca gcagcacagg gccatcgccg atgggggtat
 3181 tctgctggta gtggtcggcc agctgcacgc tgccatcctc gatgttgtgg cggatcttga
 3241 agttcacctt gatgccattc ttggccttgt cggtcatgat gtacacattg tgggcgttgt
 3301 agttgtactc catcttattg cccaggatgt tgccatcctc cttgaaatcg gtgccggtca
 3361 gctcgatgcg attcaccagg gtatcgccct cgaacttcac ctcggcgcgc gacttgtagt
 3421 tgccgtcatc ctcgaagaag atggtgcgct cctggatgta gccctcaggc atggcgctct
 3481 tgaagaagtc gtgctgcttc atgtgatcgg ggtagcgtga gaagcactgc acgccgtagc
 3541 tcagggtggt caccagggtg ggccagggca caggcagctt gccggtggtg cagatgaact
 3601 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccgctcacg ctgaacttgt
 3661 ggccattcac atcgccattc agctcgatca ggatgggcac gatgccggtg aacagctcgg
 3721 cgcccttgct caccatggtg gcgaattctc caggcgatct gacggttcac taaacgagct
 3781 ctgcttatat aggcctccca ccgtacacgc cacctcgaca ta
//


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