PA-GFPmt

LOCUS       PA-GFPmt     7956 bp DNA     circular SYN 20-NOV-2024
DEFINITION  TSN20240521-0571-00019_571-1_20240522-BAN43_E02_E02_i38.3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 16, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    483..600
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      985..994
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             991..1707
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1737..2325
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2208..2219)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2463..2481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2505..2523
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2547..2565
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2589..2607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     gap             2620..2717
                     /estimated_length=98
     LTR             2746..2926
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2951..2969)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        3757..3861
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3862..4722
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    4723..5663
                     /label=ColE1
                     /note="ColE1"
     rep_origin      4893..5481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        5727..6056
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5907..6042
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7190..7255
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7385..7405
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7830..7951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag
       61 ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca
      121 aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc
      181 gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg
      241 ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg
      301 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
      361 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
      421 tgtgctgtct catcattttg gcaaagaatt aaatttaatt aatctcgacg gtatcggtta
      481 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
      541 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
      601 atcgatcacg agactagcct cgaggtttaa actacgggat cccccggggc caccatgcgc
      661 gccccttctg ctagggcgct actgctgatt ccgcgtcggg gccctgccgt gcgagcgtgg
      721 gccccggccg tctcctctcg gatatggctg gcttctgaat ggaccccgct cgtacgcgcg
      781 tggacctctc tgatccacaa gccgggttcg ggcctccgct ttcccgcgcc cctatccggg
      841 ctgcctggcg gcgtggggca gtgggccacc tcctcggggg cccgcaggtg ctgggtgctg
      901 gcaggacccc gcgccgcaca tcccctgttc gccaggctcc agggtgcagc tgccaccggt
      961 gtgcgagacc gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     1021 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1081 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1141 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcagcta cggcgtgcag
     1201 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1261 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1321 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1381 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1441 gtctatatca tggccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
     1501 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1561 gacggccccg tgctgctgcc cgacaaccac tacctgagcc accagtccgc cctgagcaaa
     1621 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1681 actctcggca tggacgagct gtacaagtaa gaattcgatg ggactagtca tatgataatc
     1741 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     1801 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     1861 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     1921 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     1981 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     2041 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2101 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     2161 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     2221 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     2281 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcgg tacgtatggc
     2341 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     2401 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct cgagtttacc
     2461 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2521 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2581 tttaccactc cctatcagtg atagagaaaa gtgaaagtcn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnccg gatcgatctg ctttttgctt gtactgggtc tctctggtta
     2761 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2821 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2881 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa
     2941 ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
     3001 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
     3061 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
     3121 ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca
     3181 gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
     3241 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
     3301 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
     3361 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
     3421 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg
     3481 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     3541 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3601 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     3661 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     3721 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3781 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3841 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3901 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3961 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4021 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4081 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4141 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4201 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4261 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4321 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4381 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4441 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4501 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4561 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4621 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4681 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4741 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4801 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4861 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4921 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4981 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5041 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5101 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5161 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5221 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5281 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5341 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5401 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5461 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5521 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5581 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5641 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     5701 ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     5761 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     5821 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5881 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     5941 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6001 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6061 ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
     6121 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
     6181 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
     6241 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
     6301 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
     6361 agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
     6421 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     6481 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     6541 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     6601 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg
     6661 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     6721 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca
     6781 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
     6841 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
     6901 ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac
     6961 cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt
     7021 attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac
     7081 cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg
     7141 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     7201 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     7261 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     7321 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     7381 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
     7441 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
     7501 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
     7561 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
     7621 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
     7681 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
     7741 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     7801 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     7861 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7921 gtggtttgtc caaactcatc aatgtatctt atcatg
//