pENTR1A

价格:800元

联系方式:I47-825O-882O

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pENTR1A载体基本信息

出品公司: Invitrogen
载体名称: pENTR1A
质粒类型: Gateway载体;入门载体
高拷贝/低拷贝: 高拷贝
启动子:
克隆方法: 限制性内切酶;多克隆位点
载体大小: 2717 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签:
载体抗性: Kanamycin.html' target='_blank'>卡那霉素
真核筛选标记:
克隆菌株: DH5aTOP10感受态细胞
备注: --
产品目录号: 11813-011
病毒/非病毒: 非病毒

pENTR1A载体质粒图谱和多克隆位点信息

pENTR 1A载体图谱



pENTR 1A多克隆位点

pENTR1A载体简介

pENTR1A载体序列

LOCUS       pENTR1A	2717 bp 	DNA	SYN
DEFINITION  pENTR1A
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="pENTR1A"
                     /mol_type="other DNA"
     terminator      106..149
                     /label="rrnB_T1_terminator"
     terminator      281..308
                     /label="rrnB_T2_terminator"
     misc_feature    complement(358..441)
                     /label="attL2"
     misc_feature    complement(358..457)
                     /label="attL1"
     misc_feature    612..911
                     /label="ccdB"
     misc_feature    946..1045
                     /label="attL2"
     gene            1168..1977
                     /label="KanR2 (variant)"
                     /gene="KanR2 (variant)"
     CDS             1168..1977
                     /label="ORF frame 1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     rep_origin      2051..2670
                     /label="pBR322_origin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
ORIGIN
    1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG
   61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
  121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
  181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG
  241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT
  301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA
  361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC
  421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTTTA AAGGAACCAA TTCAGTCGAC
  481 TGGATCCGGT ACCGAATTCG CTTACTAAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT
  541 GATTTTTGCG GTATAAGAAT ATATACTGAT ATGTATACCC GAAGTATGTC AAAAAGAGGT
  601 GTGCTTCTAG AATGCAGTTT AAGGTTTACA CCTATAAAAG AGAGAGCCGT TATCGTCTGT
  661 TTGTGGATGT ACAGAGTGAT ATTATTGACA CGCCCGGGCG ACGGATAGTG ATCCCCCTGG
  721 CCAGTGCACG TCTGCTGTCA GATAAAGTCT CCCGTGAACT TTACCCGGTG GTGCATATCG
  781 GGGATGAAAG CTGGCGCATG ATGACCACCG ATATGGCCAG TGTGCCGGTC TCCGTTATCG
  841 GGGAAGAAGT GGCTGATCTC AGCCACCGCG AAAATGACAT CAAAAACGCC ATTAACCTGA
  901 TGTTCTGGGG AATATAGAAT TCGCGGCCGC ACTCGAGATA TCTAGACCCA GCTTTCTTGT
  961 ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA
 1021 TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGCAGC TCTGGCCCGT GTCTCAAAAT
 1081 CTCTGATGTT ACATTGCACA AGATAAAAAT ATATCATCAT GAACAATAAA ACTGTCTGCT
 1141 TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG
 1201 CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC
 1261 GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT
 1321 CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC
 1381 TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT
 1441 GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT
 1501 CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT CCCTGCGCCG GTTGCATTCG
 1561 ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA
 1621 TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG
 1681 CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC
 1741 GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT
 1801 TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG
 1861 AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT
 1921 GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA
 1981 GAATTGGTTA ATTGGTTGTA ACATTATTCA GATTGGGCCC CGTTCCACTG AGCGTCAGAC
 2041 CCGGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC
 2101 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA
 2161 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA
 2221 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT
 2281 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG
 2341 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC
 2401 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA
 2461 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG
 2521 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT
 2581 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG
 2641 CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG
 2701 CCTTTTGCTC ACATGTT
//

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