pWJ68

价格:1000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: pwtCas9-bacteria
质粒类型: CRISPR/Cas9载体;细菌基因敲除载体
高拷贝/低拷贝: 低拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: --
载体大小: 9622 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: rpoZ (C ter)
载体抗性: Chloramphenicol
克隆菌株: Top10
宿主细胞(系): 细菌,如大肠杆菌
备注:  
产品目录号: #46571
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

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载体质粒图谱和多克隆位点信息

pWJ68载体图谱



载体简介

载体序列

LOCUS       pWJ68	9622 bp 	DNA	SYN
DEFINITION  pWJ68
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9622
                     /organism="pWJ68"
                     /mol_type="other DNA"
     gene            complement(4..219)
                     /label="CAT/CamR (659 - 444)"
                     /gene="CAT/CamR (659 - 444)"
     rep_origin      581..1493
                     /label="p15A_origin"
     CDS             2245..6627
                     /label="ORF frame 1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRRAADKKYSIGLA
                     IGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTA
                     RRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEV
                     AYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKL
                     FIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI
                     ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAI
                     LLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNG
                     YAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHL
                     GELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETIT
                     PWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTE
                     GMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNAS
                     LGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVM
                     KQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKE
                     DIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMA
                     RENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDM
                     YVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMK
                     NYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSR
                     MNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTA
                     LIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLAN
                     GEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILP
                     KRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELAL
                     PSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADAN
                     LDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD*"
     gene            7066..8148
                     /label="tet (variant)"
                     /gene="tet (variant)"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRRAADKKYSIGLA
                     IGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTA
                     RRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEV
                     AYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKL
                     FIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI
                     ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAI
                     LLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNG
                     YAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHL
                     GELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETIT
                     PWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTE
                     GMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNAS
                     LGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVM
                     KQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKE
                     DIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMA
                     RENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDM
                     YVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMK
                     NYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSR
                     MNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTA
                     LIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLAN
                     GEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILP
                     KRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELAL
                     PSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADAN
                     LDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD*"
     CDS             7120..8148
                     /label="ORF frame 1"
                     /translation="MQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWI
                     LYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGA
                     ISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAA
                     LMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATK
                     RFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDD
                     DHQGQLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRG
                     AWSRATST*"
     misc_feature    complement(7310..7329)
                     /label="pBRrevBam_primer"
                     /translation="MQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWI
                     LYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGA
                     ISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAA
                     LMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATK
                     RFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDD
                     DHQGQLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRG
                     AWSRATST*"
     CDS             complement(7495..7953)
                     /label="ORF frame 2"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
     gene            complement(9182..219)
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
     CDS             complement(9182..219)
                     /label="ORF frame 2"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
ORIGIN
    1 GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT
   61 GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT
  121 ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA
  181 TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA
  241 AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT
  301 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC AGGGCTTCCC
  361 GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG TGATCTTCCG TCACAGGTAT
  421 TTATTCGGCG CAAAGTGCGT CGGGTGATGC TGCCAACTTA CTGATTTAGT GTATGATGGT
  481 GTTTTTGAGG TGCTCCAGTG GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG
  541 ACGGGGTGGT GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT
  601 GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG CAGGAGAAAA
  661 AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA TATATTCCGC TTCCTCGCTC
  721 ACTGACTCGC TACGCTCGGT CGTTCGACTG CGGCGAGCGG AAATGGCTTA CGAACGGGGC
  781 GGAGATTTCC TGGAAGATGC CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA
  841 AGCCGTTTTT CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC
  901 AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGCGGCTCCC
  961 TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT TTACCGGTGT CATTCCGCTG TTATGGCCGC
 1021 GTTTGTCTCA TTCCACGCCT GACACTCAGT TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC
 1081 TGTATGCACG AACCCCCCGT TCAGTCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
 1141 GAGTCCAACC CGGAAAGACA TGCAAAAGCA CCACTGGCAG CAGCCACTGG TAATTGATTT
 1201 AGAGGAGTTA GTCTTGAAGT CATGCGCCGG TTAAGGCTAA ACTGAAAGGA CAAGTTTTGG
 1261 TGACTGCGCT CCTCCAAGCC AGTTACCTCG GTTCAAAGAG TTGGTAGCTC AGAGAACCTT
 1321 CGAAAAACCG CCCTGCAAGG CGGTTTTTTC GTTTTCAGAG CAAGAGATTA CGCGCAGACC
 1381 AAAACGATCT CAAGAAGATC ATCTTATTAA TCAGATAAAA TATTTCTAGA TTTCAGTGCA
 1441 ATTTATCTCT TCAAATGTAG CACCTGAAGT CAGCCCCATA CGATATAAGT TGTAATTCTC
 1501 ATGTTTGACA GCTTATCATC GATAAGCTTT AATGCGGTAG TTTATCACAG TTAAATTGCT
 1561 AACGCAGTCA GGCACCGTGT ATGAAATCTA ACAATGCGCT CATCGTCATC CTCGGCACCG
 1621 TCACCCTGGA TGCTGTAGGC ATAGGCTTGG TTATGCCGGT ACTGCCGGGC CTCTTGCGGG
 1681 ATTACGAAAT CATCCTGTGG AGCTTAGTAG GTTTAGCAAG ATGGCAGCGC CTAAATGTAG
 1741 AATGATAAAA GGATTAAGAG ATTAATTTCC CTAAAAATGA TAAAACAAGC GTTTTGAAAG
 1801 CGCTTGTTTT TTTGGTTTGC AGTCAGAGTA GAATAGAAGT ATCAAAAAAA GCACCGACTC
 1861 GGTGCCACTT TTTCAAGTTG ATAACGGACT AGCCTTATTT TAACTTGCTA TGCTGTTTTG
 1921 AATGGTTCCA ACAAGATTAT TTTATAACTT TTATAACAAA TAATCAAGGA GAAATTCAAA
 1981 GAAATTTATC AGCCATAAAA CAATACTTAA TACTATAGAA TGATAACAAA ATAAACTACT
 2041 TTTTAAAAGA ATTTTGTGTT ATAATCTATT TATTATTAAG TATTGGGTAA TATTTTTTGA
 2101 AGAGATATTT TGAAAAAGAA AAATTAAAGC ATATTAAACT AATTTCGGAG GTCATTAAAA
 2161 CTATTATTGA ACAAATTGTT GGCAGACTGA ACCTGATTTC AGTATCATGC CCAGTCATTT
 2221 CTTCACCTGT GGAGCTTTTT AAGTATGGCA CGCGTAACTG TTCAGGACGC TGTAGAGAAA
 2281 ATTGGTAACC GTTTTGACCT GGTACTGGTC GCCGCGCGTC GCGCTCGTCA GATGCAGGTA
 2341 GGCGGAAAGG ATCCGCTGGT ACCGGAAGAA AACGATAAAA CCACTGTAAT CGCGCTGCGC
 2401 GAAATCGAAG AAGGTCTGAT CAACAACCAG ATCCTCGACG TTCGCGAACG CCAGGAACAG
 2461 CAAGAGCAGG AAGCCGCTGA ATTACAAGCC GTTACCGCTA TTGCTGAAGG TCGTCGTGCT
 2521 GCAGATAAGA AATACTCAAT AGGCTTAGCT ATCGGCACAA ATAGCGTCGG ATGGGCGGTG
 2581 ATCACTGATG AATATAAGGT TCCGTCTAAA AAGTTCAAGG TTCTGGGAAA TACAGACCGC
 2641 CACAGTATCA AAAAAAATCT TATAGGGGCT CTTTTATTTG ACAGTGGAGA GACAGCGGAA
 2701 GCGACTCGTC TCAAACGGAC AGCTCGTAGA AGGTATACAC GTCGGAAGAA TCGTATTTGT
 2761 TATCTACAGG AGATTTTTTC AAATGAGATG GCGAAAGTAG ATGATAGTTT CTTTCATCGA
 2821 CTTGAAGAGT CTTTTTTGGT GGAAGAAGAC AAGAAGCATG AACGTCATCC TATTTTTGGA
 2881 AATATAGTAG ATGAAGTTGC TTATCATGAG AAATATCCAA CTATCTATCA TCTGCGAAAA
 2941 AAATTGGTAG ATTCTACTGA TAAAGCGGAT TTGCGCTTAA TCTATTTGGC CTTAGCGCAT
 3001 ATGATTAAGT TTCGTGGTCA TTTTTTGATT GAGGGAGATT TAAATCCTGA TAATAGTGAT
 3061 GTGGACAAAC TATTTATCCA GTTGGTACAA ACCTACAATC AATTATTTGA AGAAAACCCT
 3121 ATTAACGCAA GTGGAGTAGA TGCTAAAGCG ATTCTTTCTG CACGATTGAG TAAATCAAGA
 3181 CGATTAGAAA ATCTCATTGC TCAGCTCCCC GGTGAGAAGA AAAATGGCTT ATTTGGGAAT
 3241 CTCATTGCTT TGTCATTGGG TTTGACCCCT AATTTTAAAT CAAATTTTGA TTTGGCAGAA
 3301 GATGCTAAAT TACAGCTTTC AAAAGATACT TACGATGATG ATTTAGATAA TTTATTGGCG
 3361 CAAATTGGAG ATCAATATGC TGATTTGTTT TTGGCAGCTA AGAATTTATC AGATGCTATT
 3421 TTACTTTCAG ATATCCTAAG AGTAAATACT GAAATAACTA AGGCTCCCCT ATCAGCTTCA
 3481 ATGATTAAAC GCTACGATGA ACATCATCAA GACTTGACTC TTTTAAAAGC TTTAGTTCGA
 3541 CAACAACTTC CAGAAAAGTA TAAAGAAATC TTTTTTGATC AATCAAAAAA CGGATATGCA
 3601 GGTTATATTG ATGGGGGAGC TAGCCAAGAA GAATTTTATA AATTTATCAA ACCAATTTTA
 3661 GAAAAAATGG ATGGTACTGA GGAATTATTG GTGAAACTAA ATCGTGAAGA TTTGCTGCGC
 3721 AAGCAACGGA CCTTTGACAA CGGCTCTATT CCCCATCAAA TTCACTTGGG TGAGCTGCAT
 3781 GCTATTTTGA GAAGACAAGA AGACTTTTAT CCATTTTTAA AAGACAATCG TGAGAAGATT
 3841 GAAAAAATCT TGACTTTTCG AATTCCTTAT TATGTTGGTC CATTGGCGCG TGGCAATAGT
 3901 CGTTTTGCAT GGATGACTCG GAAGTCTGAA GAAACAATTA CCCCATGGAA TTTTGAAGAA
 3961 GTTGTCGATA AAGGTGCTTC AGCTCAATCA TTTATTGAAC GCATGACAAA CTTTGATAAA
 4021 AATCTTCCAA ATGAAAAAGT ACTACCAAAA CATAGTTTGC TTTATGAGTA TTTTACGGTT
 4081 TATAACGAAT TGACAAAGGT CAAATATGTT ACTGAAGGAA TGCGAAAACC AGCATTTCTT
 4141 TCAGGTGAAC AGAAGAAAGC CATTGTTGAT TTACTCTTCA AAACAAATCG AAAAGTAACC
 4201 GTTAAGCAAT TAAAAGAAGA TTATTTCAAA AAAATAGAAT GTTTTGATAG TGTTGAAATT
 4261 TCAGGAGTTG AAGATAGATT TAATGCTTCA TTAGGTACCT ACCATGATTT GCTAAAAATT
 4321 ATTAAAGATA AAGATTTTTT GGATAATGAA GAAAATGAAG ATATCTTAGA GGATATTGTT
 4381 TTAACATTGA CCTTATTTGA AGATAGGGAG ATGATTGAGG AAAGACTTAA AACATATGCT
 4441 CACCTCTTTG ATGATAAGGT GATGAAACAG CTTAAACGTC GCCGTTATAC TGGTTGGGGA
 4501 CGTTTGTCTC GAAAATTGAT TAATGGTATT AGGGATAAGC AATCTGGCAA AACAATATTA
 4561 GATTTTTTGA AATCAGATGG TTTTGCCAAT CGCAATTTTA TGCAGCTGAT CCATGATGAT
 4621 AGTTTGACAT TTAAAGAAGA CATTCAAAAA GCACAAGTGT CTGGACAAGG CGATAGTTTA
 4681 CATGAACATA TTGCAAATTT AGCTGGTAGC CCTGCTATTA AAAAAGGTAT TTTACAGACT
 4741 GTAAAAGTTG TTGATGAATT GGTCAAAGTA ATGGGGCGGC ATAAGCCAGA AAATATCGTT
 4801 ATTGAAATGG CACGTGAAAA TCAGACAACT CAAAAGGGCC AGAAAAATTC GCGAGAGCGT
 4861 ATGAAACGAA TCGAAGAAGG TATCAAAGAA TTAGGAAGTC AGATTCTTAA AGAGCATCCT
 4921 GTTGAAAATA CTCAATTGCA AAATGAAAAG CTCTATCTCT ATTATCTCCA AAATGGAAGA
 4981 GACATGTATG TGGACCAAGA ATTAGATATT AATCGTTTAA GTGATTATGA TGTCGATGCC
 5041 ATTGTTCCAC AAAGTTTCCT TAAAGACGAT TCAATAGACA ATAAGGTCTT AACGCGTTCT
 5101 GATAAAAATC GTGGTAAATC GGATAACGTT CCAAGTGAAG AAGTAGTCAA AAAGATGAAA
 5161 AACTATTGGA GACAACTTCT AAACGCCAAG TTAATCACTC AACGTAAGTT TGATAATTTA
 5221 ACGAAAGCTG AACGTGGAGG TTTGAGTGAA CTTGATAAAG CTGGTTTTAT CAAACGCCAA
 5281 TTGGTTGAAA CTCGCCAAAT CACTAAGCAT GTGGCACAAA TTTTGGATAG TCGCATGAAT
 5341 ACTAAATACG ATGAAAATGA TAAACTTATT CGAGAGGTTA AAGTGATTAC CTTAAAATCT
 5401 AAATTAGTTT CTGACTTCCG AAAAGATTTC CAATTCTATA AAGTACGTGA GATTAACAAT
 5461 TACCATCATG CCCATGATGC GTATCTAAAT GCCGTCGTTG GAACTGCTTT GATTAAGAAA
 5521 TATCCAAAAC TTGAATCGGA GTTTGTCTAT GGTGATTATA AAGTTTATGA TGTTCGTAAA
 5581 ATGATTGCTA AGTCTGAGCA AGAAATAGGC AAAGCAACCG CAAAATATTT CTTTTACTCT
 5641 AATATCATGA ACTTCTTCAA AACAGAAATT ACACTTGCAA ATGGAGAGAT TCGCAAACGC
 5701 CCTCTAATCG AAACTAATGG GGAAACTGGA GAAATTGTCT GGGATAAAGG GCGAGATTTT
 5761 GCCACAGTGC GCAAAGTATT GTCCATGCCC CAAGTCAATA TTGTCAAGAA AACAGAAGTA
 5821 CAGACAGGCG GATTCTCCAA GGAGTCAATT TTACCAAAAA GAAATTCGGA CAAGCTTATT
 5881 GCTCGTAAAA AAGACTGGGA TCCAAAAAAA TATGGTGGTT TTGATAGTCC AACGGTAGCT
 5941 TATTCAGTCC TAGTGGTTGC TAAGGTGGAA AAAGGGAAAT CGAAGAAGTT AAAATCCGTT
 6001 AAAGAGTTAC TAGGGATCAC AATTATGGAA AGAAGTTCCT TTGAAAAAAA TCCGATTGAC
 6061 TTTTTAGAAG CTAAAGGATA TAAGGAAGTT AAAAAAGACT TAATCATTAA ACTACCTAAA
 6121 TATAGTCTTT TTGAGTTAGA AAACGGTCGT AAACGGATGC TGGCTAGTGC CGGAGAATTA
 6181 CAAAAAGGAA ATGAGCTGGC TCTGCCAAGC AAATATGTGA ATTTTTTATA TTTAGCTAGT
 6241 CATTATGAAA AGTTGAAGGG TAGTCCAGAA GATAACGAAC AAAAACAATT GTTTGTGGAG
 6301 CAGCATAAGC ATTATTTAGA TGAGATTATT GAGCAAATCA GTGAATTTTC TAAGCGTGTT
 6361 ATTTTAGCAG ATGCCAATTT AGATAAAGTT CTTAGTGCAT ATAACAAACA TAGAGACAAA
 6421 CCAATACGTG AACAAGCAGA AAATATTATT CATTTATTTA CGTTGACGAA TCTTGGAGCT
 6481 CCCGCTGCTT TTAAATATTT TGATACAACA ATTGATCGTA AACGATATAC GTCTACAAAA
 6541 GAAGTTTTAG ATGCCACTCT TATCCATCAA TCCATCACTG GTCTTTATGA AACACGCATT
 6601 GATTTGAGTC AGCTAGGAGG TGACTGAAGT ATATTTTAGA TGAAGATTAT TTCTTAATAA
 6661 CTAAAAATAT GGTATAATAC TCTTAATAAA TGCAGTAATA CAGGGGCTTT TCAAGACTGA
 6721 AGTCTAGCTG AGACAAATAG TGCGATTACG AAATTTTTTA GACAAAAATA GTCTACGAGG
 6781 TTTTAGAGCT ATGCTGTTTT GAATGGTCCC AAAACTGAGA CCAGTCTCGG AAGCTCAAAG
 6841 GTCTCGTTTT AGAGCTATGC TGTTTTGAAT GGTCCCAAAA CTTCAGCACA CTGAGACTTG
 6901 TTGAGTTCCA TGTTTTAGAG CTATGCTGTT TTGAATGGAC TCCATTCAAC ATTGCCGATG
 6961 ATAACTTGAG AAAGAGGGTT AATACCAGCA GTCGGATACC TTCCTATTCT TTCTGTTAAA
 7021 GCGTTTTCAT GTTATAATAG GCAAAAGAAG AGTAGTGTGA TCGTCCATTC CGACAGCATC
 7081 GCCAGTCACT ATGGCGTGCT GCTAGCGCTA TATGCGTTGA TGCAATTTCT ATGCGCACCC
 7141 GTTCTCGGAG CACTGTCCGA CCGCTTTGGC CGCCGCCCAG TCCTGCTCGC TTCGCTACTT
 7201 GGAGCCACTA TCGACTACGC GATCATGGCG ACCACACCCG TCCTGTGGAT CCTCTACGCC
 7261 GGACGCATCG TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC
 7321 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC
 7381 GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA
 7441 CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG
 7501 CAGGAGTCGC ATAAGGGAGA GCGTCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC
 7561 TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC
 7621 ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTGGGTCA TTTTCGGCGA GGACCGCTTT
 7681 CGCTGGAGCG CGACGATGAT CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC
 7741 GCTCAAGCCT TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC
 7801 GCCGGCATGG CGGCCGACGC GCTGGGCTAC GTCTTGCTGG CGTTCGCGAC GCGAGGCTGG
 7861 ATGGCCTTCC CCATTATGAT TCTTCTCGCT TCCGGCGGCA TCGGGATGCC CGCGTTGCAG
 7921 GCCATGCTGT CCAGGCAGGT AGATGACGAC CATCAGGGAC AGCTTCAAGG ATCGCTCGCG
 7981 GCTCTTACCA GCCTAACTTC GATCATTGGA CCGCTGATCG TCACGGCGAT TTATGCCGCC
 8041 TCGGCGAGCA CATGGAACGG GTTGGCATGG ATTGTAGGCG CCGCCCTATA CCTTGTCTGC
 8101 CTCCCCGCGT TGCGTCGCGG TGCATGGAGC CGGGCCACCT CGACCTGAAT GGAAGCCGGC
 8161 GGCACCTCGC TAACGGATTC ACCACTCCAA GAATTGGAGC CAATCAATTC TTGCGGAGAA
 8221 CTGTGAATGC GCAAACCAAC CCTTGGCAGA ACATATCCAT CGCGTCCGCC ATCTCCAGCA
 8281 GCCGCACGCG GCGCATCTCG GGCAGCGTTG GGTCCTGGCC ACGGGTGCGC ATGATCGTGC
 8341 TCCTGTCGTT GAGGACCCGG CTAGGCTGGC GGGGTTGCCT TACTGGTTAG CAGAATGAAT
 8401 CACCGATACG CGAGCGAACG TGAAGCGACT GCTGCTGCAA AACGTCTGCG ACCTGAGCAA
 8461 CAACATGAAT GGTCTTCGGT TTCCGTGTTT CGTAAAGTCT GGAAACGCGG AAGTCCCCTA
 8521 CGTGCTGCTG AAGTTGCCCG CAACAGAGAG TGGAACCAAC CGGTGATACC ACGATACTAT
 8581 GACTGAGAGT CAACGCCATG AGCGGCCTCA TTTCTTATTC TGAGTTACAA CAGTCCGCAC
 8641 CGCTGTCCGG TAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC GCACTTATGA
 8701 CTGTCTTCTT TATCATGCAA CTCGTAGGAC AGGTGCCGGC AGCGCCCAAC AGTCCCCCGG
 8761 CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCC CTGCACCATT ATGTTCCGGA
 8821 TCTGCATCGC AGGATGCTGC TGGCTACCCT GTGGAACACC TACATCTGTA TTAACGAAGC
 8881 GCTAACCGTT TTTATCAGGC TCTGGGAGGC AGAATAAATG ATCATATCGT CAATTATTAC
 8941 CTCCACGGGG AGAGCCTGAG CAAACTGGCC TCAGGCATTT GAGAAGCACA CGGTCACACT
 9001 GCTTCCGGTA GTCAATAAAC CGGTAAACCA GCAATAGACA TAAGCGGCTA TTTAACGACC
 9061 CTGCCCTGAA CCGACGACCG GGTCGAATTT GCTTTCGAAT TTCTGCCATT CATCCGCTTA
 9121 TTATCACTTA TTCAGGCGTA GCACCAGGCG TTTAAGGGCA CCAATAACTG CCTTAAAAAA
 9181 ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA
 9241 CATGGAAGCC ATCACAGACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT
 9301 CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG
 9361 CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT
 9421 TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG
 9481 AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG
 9541 TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT
 9601 CACCGTCTTT CATTGCCATA CG
//

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