| 质粒名称: | Desmoplakin II no tension control (F40-based) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 118716 |
| 存储实验室: | Carsten Grashoff |
|
载体骨架基本信息 |
|
| 载体名称: | pLPCX |
| 载体出品公司: | Clontech |
| 空载体大小: | 6265bp |
| 完整质粒大小: | 11830 bp |
| 载体类型: | 哺乳细胞表达、Retroviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | DPII-ctrl / DPII-ctrl (YF40C) / human Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry] / Desmoplakin II no force control (F40-based) |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 5589 bp |
| 基因突变: | truncation after aa1353 |
| GenBank编号 | NM_001008844.1 |
| 启动子: | CMV |
| 标签/融合蛋白: | YPet(short)-F40-mCherry (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | ECoRI (not destroyed) |
| 3’克隆位点: | ClaI (not destroyed) |
| 5’测序引物 | AGCTcGTTTAGTGAACCGTCAGATC |
| 3’测序引物 | ACCTACAGGTGGGGTCTTTCATTCCC |
|
文献引用方法 |
|
| 材料和方法部分: | Desmoplakin II no force control (F40-based) was a gift from Carsten Grashoff (Addgene plasmid # 118716 ; http://n2t.net/addgene:118716 ; RRID:Addgene_118716) |
| 参考文献部分: | Mechanical loading of desmosomes depends on the magnitude and orientation of external stress. Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C. Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0. 10.1038/s41467-018-07523-0 PubMed 30538252 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS Desmoplakin II no tension control (F40-based) 11830 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION The no force control for the F40-based human desmoplakin II tension
sensor serves to detect changes in FRET between YPet(short) and
mCherry that are tension-independent..
ACCESSION .
VERSION .
KEYWORDS Desmoplakin II no force control (F40-based)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11830)
AUTHORS Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C
TITLE Mechanical loading of desmosomes depends on the magnitude and
orientation of external stress.
JOURNAL Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0.
PUBMED 30538252
REFERENCE 2 (bases 1 to 11830)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11830
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 414..589
/label=5' LTR (truncated)
/note="truncated long terminal repeat from Moloney murine
sarcoma virus"
misc_feature 652..851
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1052..1468
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1398..1420
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1436..1452
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 1565..2164
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1565..1584)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2061..2081
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
enhancer 2339..2642
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2643..2846
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2797..2817
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind complement(7289..7308)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
CDS 7799..8509
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(7944..7962)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 8212..8231
/label=mCherry-F
/note="mCherry, forward primer"
LTR 8569..9162
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(9265..9287)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
protein_bind complement(9415..9448)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind complement(9508..9541)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind 9561..9580
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(9661..9678)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9832..10420)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9912..9931)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10591..11451)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11214..11233
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11452..11556)
/gene="bla"
/label=AmpR promoter
primer_bind 11624..11642
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
1441 tccagccctc actccttctc taggcgccgg aatgatcccc cgggctgcag gtcggccgcc
1501 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc
1561 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
1621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
1681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
1741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
1801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
1861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
1921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
1981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
2041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
2101 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
2161 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc
2221 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac
2281 tctagtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
2881 ggactcagat ctgggcccat aaagcttata cgaattcatg agctgcaacg gaggctccca
2941 cccgcggatc aacactctgg gccgcatgat ccgcgccgag tctggcccgg acctgcgcta
3001 cgaggtgacc agcggcggcg ggggcaccag caggatgtac tattctcggc gcggcgtgat
3061 caccgaccag aactcggacg gctactgtca aaccggcacg atgtccaggc accagaacca
3121 gaacaccatc caggagctgc tgcagaactg ctccgactgc ttgatgcgag cagagctcat
3181 cgtgcagcct gaattgaagt atggagatgg aatacaactg actcggagtc gagaattgga
3241 tgagtgtttt gcccaggcca atgaccaaat ggaaatcctc gacagcttga tcagagagat
3301 gcggcagatg ggccagccct gtgatgctta ccagaaaagg cttcttcagc tccaagagca
3361 aatgcgagcc ctttataaag ccatcagtgt cccacgagtc cgcagggcca gctccaaggg
3421 tggtggaggc tacacttgtc agagtggctc tggctgggat gagttcacca aacatgtcac
3481 cagtgaatgt ttggggtgga tgaggcagca aagggcggag atggacatgg tggcctgggg
3541 tgtggacctg gcctcagtgg agcagcacat taacagccac cggggcatcc acaactccat
3601 cggcgactat cgctggcagc tggacaaaat caaagccgac ctgcgcgaga aatctgcgat
3661 ctaccagttg gaggaggagt atgaaaacct gctgaaagcg tcctttgaga ggatggatca
3721 cctgcgacag ctgcagaaca tcattcaggc cacgtccagg gagatcatgt ggatcaatga
3781 ctgcgaggag gaggagctgc tgtacgactg gagcgacaag aacaccaaca tcgctcagaa
3841 acaggaggcc ttctccatac gcatgagtca actggaagtt aaagaaaaag agctcaataa
3901 gctgaaacaa gaaagtgacc aacttgtcct caatcagcat ccagcttcag acaaaattga
3961 ggcctatatg gacactctgc agacgcagtg gagttggatt cttcagatca ccaagtgcat
4021 tgatgttcat ctgaaagaaa atgctgccta ctttcagttt tttgaagagg cgcagtctac
4081 tgaagcatac ctgaaggggc tccaggactc catcaggaag aagtacccct gcgacaagaa
4141 catgcccctg cagcacctgc tggaacagat caaggagctg gagaaagaac gagagaaaat
4201 ccttgaatac aagcgtcagg tgcagaactt ggtaaacaag tctaagaaga ttgtacagct
4261 gaagcctcgt aacccagact acagaagcaa taaacccatt attctcagag ctctctgtga
4321 ctacaaacaa gatcagaaaa tcgtgcataa gggggatgag tgtatcctga aggacaacaa
4381 cgagcgcagc aagtggtacg tgacgggccc gggaggcgtt gacatgcttg ttccctctgt
4441 ggggctgatc atccctcctc cgaacccact ggccgtggac ctctcttgca agattgagca
4501 gtactacgaa gccatcttgg ctctgtggaa ccagctctac atcaacatga agagcctggt
4561 gtcctggcac tactgcatga ttgacataga gaagatcagg gccatgacaa tcgccaagct
4621 gaaaacaatg cggcaggaag attacatgaa gacgatagcc gaccttgagt tacattacca
4681 agagttcatc agaaatagcc aaggctcaga gatgtttgga gatgatgaca agcggaaaat
4741 acagtctcag ttcaccgatg cccagaagca ttaccagacc ctggtcattc agctccctgg
4801 ctatccccag caccagacag tgaccacaac tgaaatcact catcatggaa cctgccaaga
4861 tgtcaaccat aataaagtaa ttgaaaccaa cagagaaaat gacaagcaag aaacatggat
4921 gctgatggag ctgcagaaga ttcgcaggca gatagagcac tgcgagggca ggatgactct
4981 caaaaacctc cctctagcag accaggggtc ttctcaccac atcacagtga aaattaacga
5041 gcttaagagt gtgcagaatg attcacaagc aattgctgag gttctcaacc agcttaaaga
5101 tatgcttgcc aacttcagag gttctgaaaa gtactgctat ttacagaatg aagtatttgg
5161 actatttcag aaactggaaa atatcaatgg tgttacagat ggctacttaa atagcttatg
5221 cacagtaagg gcactgctcc aggctattct ccaaacagaa gacatgttaa aggtttatga
5281 agccaggctc actgaggagg aaactgtctg cctggacctg gataaagtgg aagcttaccg
5341 ctgtggactg aagaaaataa aaaatgactt gaacttgaag aagtcgttgt tggccactat
5401 gaagacagaa ctacagaaag cccagcagat ccactctcag acttcacagc agtatccact
5461 ttatgatctg gacttgggca agttcggtga aaaagtcaca cagctgacag accgctggca
5521 aaggatagat aaacagatcg actttagatt atgggacctg gagaaacaaa tcaagcaatt
5581 gaggaattat cgtgataact atcaggcttt ctgcaagtgg ctctatgatg ctaaacgccg
5641 ccaggattcc ttagaatcca tgaaatttgg agattccaac acagtcatgc ggtttttgaa
5701 tgagcagaag aacttgcaca gtgaaatatc tggcaaacga gacaaatcag aggaagtaca
5761 aaaaattgct gaactttgcg ccaattcaat taaggattat gagctccagc tggcctcata
5821 cacctcagga ctggaaactc tgctgaacat acctatcaag aggaccatga ttcagtcccc
5881 ttctggggtg attctgcaag aggctgcaga tgttcatgct cggtacattg aactacttac
5941 aagatctgga gactattaca ggttcttaag tgagatgctg aagagtttgg aagatctgaa
6001 gctgaaaaat accaagatcg aagttttgga agaggagctc agactggccc gagatgccaa
6061 ctcggaaaac tgtaataaga acaaattcct ggatcagaac ctgcagaaat accaggcaga
6121 gtgttcccag ttcaaagcga agcttgcgag cctggaggag ctgaagagac aggctgagct
6181 ggatgggaag tcggctaagc aaaatctaga caagtgctac ggccaaataa aagaactcaa
6241 tgagaagatc acccgactga cttatgagat tgaagatgaa aagagaagaa gaaaatctgt
6301 ggaagacaga tttgaccaac agaagaatga ctatgaccaa ctgcagaaag caaggcaatg
6361 tgaaaaggag aaccttggtt ggcagaaatt agagtctgag aaagccatca aggagaagga
6421 gtacgagatt gaaaggttga gggttctact gcaggaagaa ggcacccgga agagagaata
6481 tgaaaatgag ctggcaaagg catctaatag gattcaggaa tcaaagaatc agtgtactca
6541 ggtggtacag gaaagagaga gccttctggt gaaaatcaaa gtcctggagc aagacaaggc
6601 aaggctgcag aggctggagg atgagctgaa tcgtgcaaaa tcaactctag aggcagaaac
6661 cagggtgaaa cagcgcctgg agtgtgagaa acagcaaatt cagaatgacc tgaatcagtg
6721 gaagactcaa tattcccgca aggaggaggc tattaggaag atagaatcgg aaagagaaaa
6781 gagtgagaga gagaagaaca gtcttaggag tgagatcgaa agactccaag cagagatcaa
6841 gagaattgaa gagaggtgca ggcgtaagct ggaggattct accagggaga cacagtcaca
6901 gttagaaaca gaacgctccc gatatcagag ggagattgat aaactcagac agcgcccata
6961 tgggtcccat cgagaggtcg agatggtgag caaaggcgaa gagctgttca ccggcgtggt
7021 gcccatcctg gtggagctgg acggcgacgt gaacggccac aagttcagcg tgagcggcga
7081 gggcgagggc gacgccacct acggcaagct gaccctgaag ctgctgtgca ccaccggcaa
7141 gctgcccgtg ccctggccca ccctggtgac caccctgggc tacggcgtgc agtgcttcgc
7201 ccggtacccc gaccacatga agcagcacga cttcttcaag agcgccatgc ccgagggcta
7261 cgtgcaggag cggaccatct tcttcaagga cgacggcaac tacaagaccc gggccgaggt
7321 gaagttcgag ggcgacaccc tggtgaaccg gatcgagctg aagggcatcg acttcaagga
7381 ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca acgtgtacat
7441 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccggc acaacatcga
7501 ggacggcggc gtgcagctgg ccgaccacta ccagcagaac acccccatcg gcgacggccc
7561 cgtgctgctg cccgacaacc actacctgag ctaccagagc gccctgttca aggaccccaa
7621 cgagaagcgg gaccacatgg tgctgctgga gttcctgacc gccgccggat ccgggccagg
7681 tggagcaggg ccaggtggtg cagggccagg tggtgcaggg ccaggtggtg cagggcccgg
7741 tggtgcaggt ccaggtggtg caggtccagg tggtgctggt ccaggtggtg ctggatccat
7801 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
7861 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
7921 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
7981 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
8041 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
8101 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
8161 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
8221 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
8281 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
8341 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
8401 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
8461 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgccta
8521 tacatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc
8581 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac
8641 ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg
8701 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
8761 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
8821 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca
8881 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
8941 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
9001 acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
9061 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
9121 tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat
9181 cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg gctgcctcgc
9241 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
9301 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
9361 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt actgataact
9421 tcgtataatg tatgctatac gaagttatta ggtctgaaga ggagtttacg tccagccaag
9481 ctagggccgc gatccggaac ccttaatata acttcgtata atgtatgcta tacgaagtta
9541 tcagtactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
9601 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
9661 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
9721 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
9781 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
9841 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
9901 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
9961 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
10021 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
10081 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
10141 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
10201 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
10261 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
10321 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
10381 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
10441 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
10501 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
10561 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
10621 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
10681 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
10741 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
10801 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
10861 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
10921 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
10981 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
11041 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
11101 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
11161 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
11221 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
11281 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
11341 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
11401 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
11461 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
11521 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
11581 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
11641 gccctttcgt cttcaagaat tgctagcaat tgctagcaat tgctagcaat tcataccaga
11701 tcaccgaaaa ctgtcctcca aatgtgtccc cctcacactc ccaaattcgc gggcttctgc
11761 ctcttagacc actctaccct attccccaca ctcaccggag ccaaagccgc ggcccttccg
11821 tttctttgct
//
